M S Pavelka

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. pmc Comparison of the construction of unmarked deletion mutations in Mycobacterium smegmatis, Mycobacterium bovis bacillus Calmette-Guérin, and Mycobacterium tuberculosis H37Rv by allelic exchange
    M S Pavelka
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Bacteriol 181:4780-9. 1999
  2. ncbi request reprint Another brick in the wall
    Martin S Pavelka
    Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Ave, Box 672, Rochester, NY 14642, USA
    Trends Microbiol 15:147-9. 2007
  3. pmc Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis
    M S Pavelka
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Bacteriol 179:2777-82. 1997
  4. ncbi request reprint Characterization of KpsT, the ATP-binding component of the ABC-transporter involved with the export of capsular polysialic acid in Escherichia coli K1
    M S Pavelka
    Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642
    J Biol Chem 269:20149-58. 1994
  5. ncbi request reprint Extragenic suppression of the requirement for diaminopimelate in diaminopimelate auxotrophs of Mycobacterium smegmatis
    Sandra A Consaul
    Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    FEMS Microbiol Lett 225:131-5. 2003
  6. pmc An unusual mutation results in the replacement of diaminopimelate with lanthionine in the peptidoglycan of a mutant strain of Mycobacterium smegmatis
    Sandra A Consaul
    University of Rochester Medical Center, Department of Microbiology and Immunology, 601 Elmwood Ave, Box 672, Rochester, NY 14642, USA
    J Bacteriol 187:1612-20. 2005
  7. pmc Identification of two genes, kpsM and kpsT, in region 3 of the polysialic acid gene cluster of Escherichia coli K1
    M S Pavelka
    Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642
    J Bacteriol 173:4603-10. 1991
  8. pmc Characterization of novel Mycobacterium tuberculosis and Mycobacterium smegmatis mutants hypersusceptible to beta-lactam antibiotics
    Anthony R Flores
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Bacteriol 187:1892-900. 2005
  9. ncbi request reprint Identification of the namH gene, encoding the hydroxylase responsible for the N-glycolylation of the mycobacterial peptidoglycan
    Jon B Raymond
    Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 280:326-33. 2005
  10. ncbi request reprint A method for the enzymatic synthesis and HPLC purification of the peptidoglycan precursor UDP-N-acetylmuramic acid
    Jon B Raymond
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Ave, Box 672, Rochester, NY 14642, USA
    FEMS Microbiol Lett 229:83-9. 2003

Research Grants

Collaborators

  • Neil P Price
  • Sandra A Consaul
  • Jon B Raymond
  • Sebabrata Mahapatra
  • Dean C Crick
  • Maria Magdalena Patru
  • Eleanor Z Kincaid
  • Anthony R Flores
  • Ludovic Desvignes
  • Andrea J Wolf
  • Patrick J Brennan
  • Joel D Ernst
  • Lori F Wright
  • Linda M Parsons
  • William R Jacobs

Detail Information

Publications12

  1. pmc Comparison of the construction of unmarked deletion mutations in Mycobacterium smegmatis, Mycobacterium bovis bacillus Calmette-Guérin, and Mycobacterium tuberculosis H37Rv by allelic exchange
    M S Pavelka
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Bacteriol 181:4780-9. 1999
    ..The mutants described in this work are potential vaccine candidates and can also be used for studies of cell wall biosynthesis and amino acid metabolism...
  2. ncbi request reprint Another brick in the wall
    Martin S Pavelka
    Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Ave, Box 672, Rochester, NY 14642, USA
    Trends Microbiol 15:147-9. 2007
    ..A recent study has revealed that a functional pathway for meso-diaminopimelate, one of the missing bricks for the wall, exists in chlamydiae. Here, I review the chlamydial cell wall paradox and discuss the importance of this new finding...
  3. pmc Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis
    M S Pavelka
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Bacteriol 179:2777-82. 1997
    ..Analyses of the DapB proteins from different bacterial species suggest that two different classes of DHPR enzymes may exist in bacteria...
  4. ncbi request reprint Characterization of KpsT, the ATP-binding component of the ABC-transporter involved with the export of capsular polysialic acid in Escherichia coli K1
    M S Pavelka
    Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642
    J Biol Chem 269:20149-58. 1994
    ..The results obtained from chemical mutagenesis of kpsT are consistent with the model and revealed characteristics particular to capsule transporters...
  5. ncbi request reprint Extragenic suppression of the requirement for diaminopimelate in diaminopimelate auxotrophs of Mycobacterium smegmatis
    Sandra A Consaul
    Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    FEMS Microbiol Lett 225:131-5. 2003
    ..Here we report the isolation and characterization of seven classes of spontaneous M. smegmatis mutants with extragenic mutations that can suppress the DAP requirement of DAP auxotrophs...
  6. pmc An unusual mutation results in the replacement of diaminopimelate with lanthionine in the peptidoglycan of a mutant strain of Mycobacterium smegmatis
    Sandra A Consaul
    University of Rochester Medical Center, Department of Microbiology and Immunology, 601 Elmwood Ave, Box 672, Rochester, NY 14642, USA
    J Bacteriol 187:1612-20. 2005
    ....
  7. pmc Identification of two genes, kpsM and kpsT, in region 3 of the polysialic acid gene cluster of Escherichia coli K1
    M S Pavelka
    Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642
    J Bacteriol 173:4603-10. 1991
    ..We propose that KpsM and KpsT constitute a system for transport of polysialic acid across the cytoplasmic membrane...
  8. pmc Characterization of novel Mycobacterium tuberculosis and Mycobacterium smegmatis mutants hypersusceptible to beta-lactam antibiotics
    Anthony R Flores
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Bacteriol 187:1892-900. 2005
    ..Two of the peptidoglycan-biosynthetic genes (ponA2 and pbpX) may encode beta-lactam antibiotic-resistant enzymes proposed to be involved with the synthesis of the unusual diaminopimelyl linkages within the mycobacterial peptidoglycan...
  9. ncbi request reprint Identification of the namH gene, encoding the hydroxylase responsible for the N-glycolylation of the mycobacterial peptidoglycan
    Jon B Raymond
    Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 280:326-33. 2005
    ..Our studies suggest that the N-glycolylation of mycobacterial peptidoglycan may play a role in lysozyme resistance or may contribute to the structural stability of the cell wall architecture...
  10. ncbi request reprint A method for the enzymatic synthesis and HPLC purification of the peptidoglycan precursor UDP-N-acetylmuramic acid
    Jon B Raymond
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Ave, Box 672, Rochester, NY 14642, USA
    FEMS Microbiol Lett 229:83-9. 2003
    ..The identity of the UDP-MurNAc synthesized by our method was confirmed by electrospray ionization mass spectrometry. Furthermore, we show that the UDP-MurNAc can support a UDP-MurNAc-L-alanine ligase reaction...
  11. pmc A role for the class A penicillin-binding protein PonA2 in the survival of Mycobacterium smegmatis under conditions of nonreplication
    Maria Magdalena Patru
    University of Rochester Medical Center, Department of Microbiology and Immunology, 601 Elmwood Ave, Rochester, NY 14642, USA
    J Bacteriol 192:3043-54. 2010
    ..However, the regulation of PonA3 is likely different, suggesting that its importance could be related to stresses encountered in the environmental niches occupied by M. smegmatis and other soil-dwelling mycobacteria...
  12. ncbi request reprint Codominance of TLR2-dependent and TLR2-independent modulation of MHC class II in Mycobacterium tuberculosis infection in vivo
    Eleanor Z Kincaid
    Division of Infectious Diseases, Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    J Immunol 179:3187-95. 2007
    ..tuberculosis uses multiple pathways to abrogate the action of an important effector of adaptive immunity. This work was supported by National Institutes of Health Grants AI 065357-AI 020010...

Research Grants13

  1. Identification of secreted proteins important for tularemia pathogenesis
    Martin Pavelka; Fiscal Year: 2007
    ..Because of F. tularensis is a select agent and could be used a biological weapon, this research could potentially have a significant impact on public health in the future. ..
  2. Genetic screens to identify mycobacterial porins
    Martin Pavelka; Fiscal Year: 2007
    ..This would be an important contribution to reduce the public health burden of mycobacterial disease in the United States. ..
  3. Development of genetic tools for Francisella tularensis
    Martin Pavelka; Fiscal Year: 2004
    ..The further development of Francisella genetics is timely, as the sequencing of the genome of the F. tularensis subsp. tularensis, strain Schu 4 is almost complete. ..
  4. BIOSYNTHESIS OF THE MYCOBACTERIAL PEPTIDOGLYCAN
    Martin Pavelka; Fiscal Year: 2004
    ..For the aims of this proposal, the Pi will study M. tuberculosis and M. smegmatis as a model organism using the techniques of classical bacterial genetics, molecular biology and biochemistry. ..
  5. Assembly of the mycobacterial cell wall
    Martin S Pavelka; Fiscal Year: 2010
    ..Understanding the biology of these cross-links could lead to the development of new antibiotics for the management of latent tuberculosis, which would have a significant impact upon public health. ..