Sri Rama Prasanna Pavani

Summary

Affiliation: University of Colorado
Country: USA

Publications

  1. pmc Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function
    Sri Rama Prasanna Pavani
    Department of Electrical and Computer Engineering, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 106:2995-9. 2009
  2. ncbi request reprint Quantitative structured-illumination phase microscopy
    Sri Rama Prasanna Pavani
    Micro Optical Imaging Systems Laboratory, Department of Electrical and Computer Engineering, University of Colorado, Campus Box 425, Boulder, Colorado 80309, USA
    Appl Opt 47:15-24. 2008
  3. ncbi request reprint High-efficiency rotating point spread functions
    Sri Rama Prasanna Pavani
    Department of Electrical and Computer Engineering, University of Colorado, Boulder, CO 80309, USA
    Opt Express 16:3484-9. 2008
  4. ncbi request reprint Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system
    Sri Rama Prasanna Pavani
    Department of Electrical and Computer Engineering, University of Colorado, Boulder, CO 80309, USA
    Opt Express 16:22048-57. 2008
  5. doi request reprint Polarization sensitive, three-dimensional, single-molecule imaging of cells with a double-helix system
    Sri Rama Prasanna Pavani
    Department of Electrical and Computer Engineering, University of Colorado, Boulder, CO 80309, USA
    Opt Express 17:19644-55. 2009
  6. doi request reprint Three-dimensional parallel particle manipulation and tracking by integrating holographic optical tweezers and engineered point spread functions
    Donald B Conkey
    Department of Electrical, Computer and Energy Engineering, University of Colorado, Boulder, CO 80309, USA
    Opt Express 19:3835-42. 2011
  7. doi request reprint Performance limits on three-dimensional particle localization in photon-limited microscopy
    Ginni Grover
    Department of Electrical, Computer and Energy Engineering, University of Colorado, Boulder, Colorado 80309, USA
    Opt Lett 35:3306-8. 2010
  8. pmc Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions
    Sean Quirin
    Department of Electrical, Computer, and Energy Engineering, University of Colorado at Boulder, 425 UCB, Boulder, CO 80309 0425
    Proc Natl Acad Sci U S A 109:675-9. 2012

Detail Information

Publications8

  1. pmc Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function
    Sri Rama Prasanna Pavani
    Department of Electrical and Computer Engineering, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 106:2995-9. 2009
    ..The combination of optical PSF design and digital postprocessing with photoactivatable fluorophores opens up avenues for improving 3D imaging resolution beyond the Rayleigh diffraction limit...
  2. ncbi request reprint Quantitative structured-illumination phase microscopy
    Sri Rama Prasanna Pavani
    Micro Optical Imaging Systems Laboratory, Department of Electrical and Computer Engineering, University of Colorado, Campus Box 425, Boulder, Colorado 80309, USA
    Appl Opt 47:15-24. 2008
    ..A digital processing algorithm then estimates the object's quantitative OPL profile based on a closed form analytical solution, which is derived using a ray optics model for objects with small OPL gradients...
  3. ncbi request reprint High-efficiency rotating point spread functions
    Sri Rama Prasanna Pavani
    Department of Electrical and Computer Engineering, University of Colorado, Boulder, CO 80309, USA
    Opt Express 16:3484-9. 2008
    ..We observed over thirty times improvement in transfer function efficiency. Experiments with a phase-only spatial light modulator demonstrate the potential of high-efficiency rotating PSFs...
  4. ncbi request reprint Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system
    Sri Rama Prasanna Pavani
    Department of Electrical and Computer Engineering, University of Colorado, Boulder, CO 80309, USA
    Opt Express 16:22048-57. 2008
    ..The system determines the 3D position of multiple particles with a single image and tracks them over time while providing their velocities...
  5. doi request reprint Polarization sensitive, three-dimensional, single-molecule imaging of cells with a double-helix system
    Sri Rama Prasanna Pavani
    Department of Electrical and Computer Engineering, University of Colorado, Boulder, CO 80309, USA
    Opt Express 17:19644-55. 2009
    ..Further, we show that the 3D information from the two channels can be optimally combined to yield up to 30% improvement in localization precision relative to a single polarization channel system...
  6. doi request reprint Three-dimensional parallel particle manipulation and tracking by integrating holographic optical tweezers and engineered point spread functions
    Donald B Conkey
    Department of Electrical, Computer and Energy Engineering, University of Colorado, Boulder, CO 80309, USA
    Opt Express 19:3835-42. 2011
    ..The system is thus capable of unveiling complex 3D force landscapes that make it suitable for quantitative studies of interactions in colloidal systems, biological materials, and a variety of soft matter systems...
  7. doi request reprint Performance limits on three-dimensional particle localization in photon-limited microscopy
    Ginni Grover
    Department of Electrical, Computer and Energy Engineering, University of Colorado, Boulder, Colorado 80309, USA
    Opt Lett 35:3306-8. 2010
    ..The fundamental advantage of engineered point-spread function systems, like the double-helix, stems from the additional degrees of freedom available to control diffraction in three dimensions over variable regions of interest...
  8. pmc Optimal 3D single-molecule localization for superresolution microscopy with aberrations and engineered point spread functions
    Sean Quirin
    Department of Electrical, Computer, and Energy Engineering, University of Colorado at Boulder, 425 UCB, Boulder, CO 80309 0425
    Proc Natl Acad Sci U S A 109:675-9. 2012
    ..Experimental application of the phase-retrieval enabled maximum-likelihood estimator using a particular engineered PSF microscope demonstrates unmatched low-photon-count 3D wide-field single-molecule localization performance...