John S Parkinson

Summary

Affiliation: University of Utah
Country: USA

Publications

  1. ncbi request reprint Collaborative signaling by bacterial chemoreceptors
    John S Parkinson
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Curr Opin Microbiol 8:116-21. 2005
  2. pmc Ancient chemoreceptors retain their flexibility
    John S Parkinson
    Department of Biology, 257 South 1400 East, University of Utah, Salt Lake City, UT 84112 0840, USA
    Proc Natl Acad Sci U S A 104:2559-60. 2007
  3. doi request reprint Signaling mechanisms of HAMP domains in chemoreceptors and sensor kinases
    John S Parkinson
    Biology Department, University of Utah, Salt Lake City, Utah 84112, USA
    Annu Rev Microbiol 64:101-22. 2010
  4. pmc Bacterial chemotaxis: a new player in response regulator dephosphorylation
    John S Parkinson
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    J Bacteriol 185:1492-4. 2003
  5. ncbi request reprint Phenotypic suppression methods for analyzing intra- and inter-molecular signaling interactions of chemoreceptors
    Peter Ames
    Department of Biology, University of Utah, Salt Lake City, UT, USA
    Methods Enzymol 423:436-57. 2007
  6. pmc Signaling interactions between the aerotaxis transducer Aer and heterologous chemoreceptors in Escherichia coli
    Khoosheh K Gosink
    Biology Department, University of Utah, Salt Lake City, 84112, USA
    J Bacteriol 188:3487-93. 2006
  7. pmc Conformational suppression of inter-receptor signaling defects
    Peter Ames
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 103:9292-7. 2006
  8. pmc Cysteine-scanning analysis of the chemoreceptor-coupling domain of the Escherichia coli chemotaxis signaling kinase CheA
    Jinshi Zhao
    Biology Department, 257 South 1400 East, University of Utah, Salt Lake City, UT 84112, USA
    J Bacteriol 188:4321-30. 2006
  9. pmc Mutational analysis of the chemoreceptor-coupling domain of the Escherichia coli chemotaxis signaling kinase CheA
    Jinshi Zhao
    Biology Department, 257 South 1400 East, University of Utah, Salt Lake City, Utah 84112, USA
    J Bacteriol 188:3299-307. 2006
  10. pmc Different signaling roles of two conserved residues in the cytoplasmic hairpin tip of Tsr, the Escherichia coli serine chemoreceptor
    Patricia Mowery
    Biology Department, University of Utah, Salt Lake City, UT 84112, USA
    J Bacteriol 190:8065-74. 2008

Collaborators

  • Knut Jahreis
  • GERALD HAZELBAUER
  • JOSEPH FALKE
  • Shahid Khan
  • Peter Ames
  • Qin Zhou
  • Khoosheh K Gosink
  • Claudia A Studdert
  • Jinshi Zhao
  • So ichiro Nishiyama
  • Maria del Carmen Burón-Barral
  • Xue Sheng Han
  • Smiljka Kitanovic
  • Hai The Pham
  • Patricia Mowery
  • Stuart Wright
  • Sergei I Bibikov
  • Andrés Garzón
  • Yimin Zhao
  • Jeffery B Ostler
  • Bharat Walia
  • Andrew C Miller
  • Rebecca H Reiser

Detail Information

Publications29

  1. ncbi request reprint Collaborative signaling by bacterial chemoreceptors
    John S Parkinson
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Curr Opin Microbiol 8:116-21. 2005
    ..The signaling-teams within a receptor cluster may be built from trimers of receptor dimers that communicate through shared connections to their partner signaling proteins...
  2. pmc Ancient chemoreceptors retain their flexibility
    John S Parkinson
    Department of Biology, 257 South 1400 East, University of Utah, Salt Lake City, UT 84112 0840, USA
    Proc Natl Acad Sci U S A 104:2559-60. 2007
  3. doi request reprint Signaling mechanisms of HAMP domains in chemoreceptors and sensor kinases
    John S Parkinson
    Biology Department, University of Utah, Salt Lake City, Utah 84112, USA
    Annu Rev Microbiol 64:101-22. 2010
    ..A three-state, dynamic bundle signaling model best accounts for the signaling properties of chemoreceptor mutants and may apply to other transducers as well...
  4. pmc Bacterial chemotaxis: a new player in response regulator dephosphorylation
    John S Parkinson
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    J Bacteriol 185:1492-4. 2003
  5. ncbi request reprint Phenotypic suppression methods for analyzing intra- and inter-molecular signaling interactions of chemoreceptors
    Peter Ames
    Department of Biology, University of Utah, Salt Lake City, UT, USA
    Methods Enzymol 423:436-57. 2007
    ..The suppression patterns exhibit allele-specificity with respect to the compensatory residue positions and amino acid side chains, a hallmark of stereospecific protein-protein interactions...
  6. pmc Signaling interactions between the aerotaxis transducer Aer and heterologous chemoreceptors in Escherichia coli
    Khoosheh K Gosink
    Biology Department, University of Utah, Salt Lake City, 84112, USA
    J Bacteriol 188:3487-93. 2006
    ..Aer evidently evolved to operate collaboratively with high-abundance receptors but can also function without MCP assistance, provided that it can establish a suitable prestimulus swimming pattern...
  7. pmc Conformational suppression of inter-receptor signaling defects
    Peter Ames
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 103:9292-7. 2006
    ..These collaborative teams may be responsible for the high-gain signaling properties of bacterial chemoreceptors...
  8. pmc Cysteine-scanning analysis of the chemoreceptor-coupling domain of the Escherichia coli chemotaxis signaling kinase CheA
    Jinshi Zhao
    Biology Department, 257 South 1400 East, University of Utah, Salt Lake City, UT 84112, USA
    J Bacteriol 188:4321-30. 2006
    ..In addition, relative movements of the CheA domains may be involved in CheW binding, in ternary complex assembly, and in subsequent stimulus-induced conformational changes in receptor signaling complexes...
  9. pmc Mutational analysis of the chemoreceptor-coupling domain of the Escherichia coli chemotaxis signaling kinase CheA
    Jinshi Zhao
    Biology Department, 257 South 1400 East, University of Utah, Salt Lake City, Utah 84112, USA
    J Bacteriol 188:3299-307. 2006
    ..We conclude that P5 subdomain 1 is important for CheW binding and for receptor coupling control and that these processes may require substantial motions of the P5 domain relative to the neighboring P3 and P4 domains of CheA...
  10. pmc Different signaling roles of two conserved residues in the cytoplasmic hairpin tip of Tsr, the Escherichia coli serine chemoreceptor
    Patricia Mowery
    Biology Department, University of Utah, Salt Lake City, UT 84112, USA
    J Bacteriol 190:8065-74. 2008
    ..These findings demonstrate that the receptor determinants for CheA and CheW binding probably lie outside the hairpin tip of the receptor signaling domain...
  11. pmc Mutational analyses of HAMP helices suggest a dynamic bundle model of input-output signalling in chemoreceptors
    Qin Zhou
    Biology Department, University of Utah, Salt Lake City, UT 84112, USA
    Mol Microbiol 73:801-14. 2009
    ..This study shows how transmembrane signalling and HAMP input-output control could occur without the helix rotations central to the gearbox model...
  12. pmc Mutational analysis of the connector segment in the HAMP domain of Tsr, the Escherichia coli serine chemoreceptor
    Peter Ames
    Biology Department, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112, USA
    J Bacteriol 190:6676-85. 2008
    ..In contrast, lesions at L237 resulted in a variety of aberrant output patterns, suggesting a role in toggling output between signaling states...
  13. pmc Biphasic control logic of HAMP domain signalling in the Escherichia coli serine chemoreceptor
    Qin Zhou
    Biology Department, University of Utah, Salt Lake City, Utah 84112, USA
    Mol Microbiol 80:596-611. 2011
    ..The findings of this study provide strong support for a three-state dynamic bundle model of HAMP signalling in Tsr, and possibly in other bacterial transducers as well...
  14. pmc Mutational analysis of the P1 phosphorylation domain in Escherichia coli CheA, the signaling kinase for chemotaxis
    So ichiro Nishiyama
    Department of Biology, University of Utah, Salt Lake City, Utah, USA
    J Bacteriol 196:257-64. 2014
    ..However, once phosphorylated, the mutant P1 domains transferred phosphate to CheY at the wild-type rate. Thus, these P1 mutants appear to define interaction determinants for P1-P4 docking during the CheA autophosphorylation reaction. ..
  15. pmc Crosslinking snapshots of bacterial chemoreceptor squads
    Claudia A Studdert
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 101:2117-22. 2004
    ..Crosslinking in receptor trimer contact mutants correlated with their signaling behaviors, suggesting that trimers of dimers are both structural and functional precursors of chemoreceptor signaling teams in bacteria...
  16. pmc Phenol sensing by Escherichia coli chemoreceptors: a nonclassical mechanism
    Hai The Pham
    Biology Department, University of Utah, Salt Lake City, UT 84112, USA
    J Bacteriol 193:6597-604. 2011
    ..The structurally sensitive target for phenol is probably the TM bundle, but other behaviors could target other receptor elements...
  17. pmc Mutational analysis of N381, a key trimer contact residue in Tsr, the Escherichia coli serine chemoreceptor
    Khoosheh K Gosink
    Biology Department, University of Utah, Salt Lake City, UT 84112, USA
    J Bacteriol 193:6452-60. 2011
    ..These findings indicate that trimer dynamics probably are important for ternary complex assembly and that N381 may not be a direct binding determinant for CheW/CheA at the trimer periphery...
  18. pmc Mutational analysis of the control cable that mediates transmembrane signaling in the Escherichia coli serine chemoreceptor
    Smiljka Kitanovic
    Biology Department, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112, USA
    J Bacteriol 193:5062-72. 2011
    ..These results suggest a helix extension mechanism of Tsr transmembrane signaling in which TM2 piston motions influence HAMP stability by modulating the helicity of the control cable segment...
  19. pmc Collaborative signaling by mixed chemoreceptor teams in Escherichia coli
    Peter Ames
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 99:7060-5. 2002
    ..These findings indicate that bacterial chemoreceptor clusters are comprised of signaling teams, seemingly based on trimers of dimers, that can contain different receptor types acting collaboratively...
  20. pmc Insights into the organization and dynamics of bacterial chemoreceptor clusters through in vivo crosslinking studies
    Claudia A Studdert
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 102:15623-8. 2005
    ..Moreover, once complete signaling teams have formed, they no longer undergo dynamic exchange of receptor members...
  21. pmc Loss- and gain-of-function mutations in the F1-HAMP region of the Escherichia coli aerotaxis transducer Aer
    Maria del Carmen Burón-Barral
    Biology Department, University of Utah, Salt Lake City, 84112, USA
    J Bacteriol 188:3477-86. 2006
    ..Most aerotaxis-defective null mutations in these regions seemed to affect maturation only, indicating that these two interactions involve structurally distinct determinants...
  22. pmc Chemotactic signaling by an Escherichia coli CheA mutant that lacks the binding domain for phosphoacceptor partners
    Knut Jahreis
    Biology Department, University of Utah, Salt Lake City, UT 84112, USA
    J Bacteriol 186:2664-72. 2004
    ..These findings suggest that P2 is not crucial for CheA signaling specificity and that the principal determinants that favor appropriate phosphoacceptor partners, or exclude inappropriate ones, most likely reside in the P1 domain...
  23. pmc An Unorthodox Sensory Adaptation Site in the Escherichia coli Serine Chemoreceptor
    Xue Sheng Han
    Biology Department, University of Utah, Salt Lake City, Utah, USA
    J Bacteriol 196:641-9. 2014
    ..These findings are consistent with control of input-output signaling in Tsr through dynamic interplay of the structural stabilities of the HAMP and methylation bundles. ..
  24. pmc Methylation-independent aerotaxis mediated by the Escherichia coli Aer protein
    Sergei I Bibikov
    Biology Department, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112, USA
    J Bacteriol 186:3730-7. 2004
    ....
  25. ncbi request reprint A "bucket of light" for viewing bacterial colonies in soft agar
    John S Parkinson
    Department of Biology, University of Utah, Salt Lake City, UT, USA
    Methods Enzymol 423:432-5. 2007
    ..This brief chapter describes a simple and relatively inexpensive illumination device for viewing and photographing bacterial colonies in soft agar...
  26. ncbi request reprint HAMP domain structural determinants for signalling and sensory adaptation in Tsr, the Escherichia coli serine chemoreceptor
    Peter Ames
    Biology Department, University of Utah, Salt Lake City, UT, 84112, USA
    Mol Microbiol 91:875-86. 2014
    ....
  27. pmc Bacterial chemoreceptors: high-performance signaling in networked arrays
    Gerald L Hazelbauer
    Department of Biochemistry, University of Missouri Columbia, Columbia, MO 65211, USA
    Trends Biochem Sci 33:9-19. 2008
    ..These new data suggest multiple levels of molecular interactions, each of which contribute specific functional features and together create a sophisticated signaling device...
  28. ncbi request reprint In vivo crosslinking methods for analyzing the assembly and architecture of chemoreceptor arrays
    Claudia A Studdert
    Instituto de Investigaciones Biologicas, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina
    Methods Enzymol 423:414-31. 2007
    ..However, the general logic of our in vivo crosslinking approaches is readily applicable to other protein-protein interactions and other organisms, even when high-resolution structural information is not available...
  29. pmc Differential activation of Escherichia coli chemoreceptors by blue-light stimuli
    Stuart Wright
    Molecular Biology Consortium, 2201 W Campbell Park Drive, Chicago, IL 60612, USA
    J Bacteriol 188:3962-71. 2006
    ..However, time-resolved motion analysis revealed subtle kinetic differences that reflect the role of receptor cluster interactions in kinase activation-deactivation dynamics...

Research Grants27

  1. INTRACELLULAR SIGNALING BY BACTERIAL CHEMORECEPTORS
    John S Parkinson; Fiscal Year: 2010
    ....
  2. Aerotactic Signaling by the Escherichia coli Aer Protein
    John Parkinson; Fiscal Year: 2002
    ..Moreover, the existence of Aer homologs in pathogenic bacteria suggests that aerotactic behavior might play a role in virulence. Thus, a molecular understanding of Aer signal transduction could lead to new anti-infection agents. ..
  3. INTRACELLULAR SIGNALING BY BACTERIAL CHEMORECEPTORS
    John Parkinson; Fiscal Year: 2003
    ..These studies will provide important new perspectives on the issue of receptor clustering, a hallmark of biological signaling systems at every complexity level. ..
  4. Aerotactic Signaling by the Escherichia coli Aer Protein
    John Parkinson; Fiscal Year: 2003
    ..Moreover, the existence of Aer homologs in pathogenic bacteria suggests that aerotactic behavior might play a role in virulence. Thus, a molecular understanding of Aer signal transduction could lead to new anti-infection agents. ..
  5. INTRACELLULAR SIGNALING BY BACTERIAL CHEMORECEPTORS
    John Parkinson; Fiscal Year: 2004
    ..These studies will provide important new perspectives on the issue of receptor clustering, a hallmark of biological signaling systems at every complexity level. ..
  6. Aerotactic Signaling by the Escherichia coli Aer Protein
    John Parkinson; Fiscal Year: 2004
    ..Moreover, the existence of Aer homologs in pathogenic bacteria suggests that aerotactic behavior might play a role in virulence. Thus, a molecular understanding of Aer signal transduction could lead to new anti-infection agents. ..
  7. INTRACELLULAR SIGNALING BY BACTERIAL CHEMORECEPTORS
    John Parkinson; Fiscal Year: 2005
    ..These studies will provide important new perspectives on the issue of receptor clustering, a hallmark of biological signaling systems at every complexity level. ..
  8. INTRACELLULAR SIGNALING BY BACTERIAL CHEMORECEPTORS
    John Parkinson; Fiscal Year: 2006
    ..These studies will provide important new perspectives on the issue of receptor clustering, a hallmark of biological signaling systems at every complexity level. ..
  9. INTRACELLULAR SIGNALING BY BACTERIAL CHEMORECEPTORS
    John Parkinson; Fiscal Year: 2007
    ....
  10. INTRACELLULAR SIGNALING BY BACTERIAL CHEMORECEPTORS
    John Parkinson; Fiscal Year: 2009
    ....
  11. Aerotactic Signaling by the Escherichia coli Aer Protein
    John Parkinson; Fiscal Year: 2001
    ..Moreover, the existence of Aer homologs in pathogenic bacteria suggests that aerotactic behavior might play a role in virulence. Thus, a molecular understanding of Aer signal transduction could lead to new anti-infection agents. ..
  12. GENETICS OF CHEMOTACTIC BEHAVIOR IN ESCHERICHIA COLI
    John Parkinson; Fiscal Year: 1990
    ..The DNA sequence changes in selected mutants will be determined in order to delineate functional domains within the proteins. From these studies, structurefunction models will be devised that can be tested by sitespecific mutagenesis...
  13. GENETICS OF CHEMOTACTIC BEHAVIOR IN ESCHERICHIA COLI
    John Parkinson; Fiscal Year: 1991
    ..The DNA sequence changes in selected mutants will be determined in order to delineate functional domains within the proteins. From these studies, structurefunction models will be devised that can be tested by sitespecific mutagenesis...
  14. GENETICS OF CHEMOTACTIC BEHAVIOR IN ESCHERICHIA COLI
    John Parkinson; Fiscal Year: 1980
    ..The control of the methylation-demethylation reactions is being investigated by analyzing gene product interactions through reversion studies and by isolating deletion or insertion mutants totally devoid of enzymatic activity...
  15. INTRACELLULAR SIGNALING BY BACTERIAL CHEMORECEPTORS
    John Parkinson; Fiscal Year: 2009
    ....