Klearchos K Papas

Summary

Affiliation: University of Arizona
Country: USA

Publications

  1. pmc Persufflation (gaseous oxygen perfusion) as a method of heart preservation
    Thomas M Suszynski
    Division of Transplantation, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    J Cardiothorac Surg 8:105. 2013
  2. pmc Factors affecting transplant outcomes in diabetic nude mice receiving human, porcine, and nonhuman primate islets: analysis of 335 transplantations
    Gopalakrishnan Loganathan
    Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    Transplantation 95:1439-47. 2013
  3. pmc A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products
    A N Balamurugan
    Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Transplantation 93:693-702. 2012
  4. doi request reprint Prediction of pancreatic tissue densities by an analytical test gradient system before purification maximizes human islet recovery for islet autotransplantation/allotransplantation
    Takayuki Anazawa
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, 420 Delaware Street S E, Minneapolis, MN 55455, USA
    Transplantation 91:508-14. 2011
  5. doi request reprint Differences in glucose-stimulated insulin secretion in vitro of islets from human, nonhuman primate, and porcine origin
    Kate R Mueller
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN, USA
    Xenotransplantation 20:75-81. 2013
  6. doi request reprint Supplements in human islet culture: human serum albumin is inferior to fetal bovine serum
    Efstathios S Avgoustiniatos
    Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    Cell Transplant 21:2805-14. 2012
  7. doi request reprint Real-time assessment of encapsulated neonatal porcine islets prior to clinical xenotransplantation
    Jennifer P Kitzmann
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN, USA
    Xenotransplantation 19:333-6. 2012
  8. pmc Persufflation (or gaseous oxygen perfusion) as a method of organ preservation
    Thomas M Suszynski
    Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    Cryobiology 64:125-43. 2012
  9. pmc Insulin degradation by acinar cell proteases creates a dysfunctional environment for human islets before/after transplantation: benefits of α-1 antitrypsin treatment
    Gopalakrishnan Loganathan
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Transplantation 92:1222-30. 2011
  10. doi request reprint Successful human islet isolation and transplantation indicating the importance of class 1 collagenase and collagen degradation activity assay
    A N Balamurugan
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN, USA
    Transplantation 89:954-61. 2010

Collaborators

  • Henk Jan Schuurman
  • A N Balamurugan
  • Melanie L Graham
  • Gary W Cline
  • Andrew S Friberg
  • Theodore Karatzas
  • Michael P Murtaugh
  • Takeshi Yuasa
  • Ranjit John
  • Thierry Berney
  • Zhiguang Guo
  • Peter M Eckman
  • Robert B Elliott
  • Thomas Minor
  • Thomas M Suszynski
  • Bernhard J Hering
  • Gopalakrishnan Loganathan
  • David E R Sutherland
  • Kate R Mueller
  • Jennifer P Kitzmann
  • William E Scott
  • Michael D Rizzari
  • Takayuki Anazawa
  • Sajjad M Soltani
  • Efstathios S Avgoustiniatos
  • LINDA A TEMPELMAN
  • Michael J Taylor
  • Rebecca L Pongratz
  • Juan E Abrahante
  • Bradley P Weegman
  • Nicole A Atchison
  • Joana Ferrer
  • James D Fonger
  • Nicolas Chronos
  • David M Radosevich
  • Mukesh Tiwari
  • Rebecca L Hooper
  • David Er Sutherland
  • Daniel W Fraga
  • Tomohiro Tanaka
  • Lee Law
  • Connor A Lyons
  • Gina M Wildey
  • Rebecca A Nelson
  • Avik Shome
  • Marija Muzina
  • Phillip R Rozak
  • Jeffrey D Ansite
  • Kumaravel Velayutham
  • David J Kennedy
  • Shuichiro Matsumoto
  • Yukihide Yonekawa
  • Rajinder K Dawra
  • Ashok K Saluja
  • Joshua J Wilhelm
  • Christopher S Chapman
  • Louis S Kidder
  • Subbiah Pugazhenthi
  • TIMOTHY D O'BRIEN
  • Kyra Martins
  • Alexander Wiseman
  • Mark A Sanders
  • Paul W Todd
  • Kate Mueller
  • Efrosini Kokkoli
  • Wei Fan
  • Michael Tsapatsis

Detail Information

Publications17

  1. pmc Persufflation (gaseous oxygen perfusion) as a method of heart preservation
    Thomas M Suszynski
    Division of Transplantation, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    J Cardiothorac Surg 8:105. 2013
    ..Additionally, the key issues that need to be addressed before PSF were to become a widely utilized preservation strategy prior to clinical heart transplantation are summarized and discussed...
  2. pmc Factors affecting transplant outcomes in diabetic nude mice receiving human, porcine, and nonhuman primate islets: analysis of 335 transplantations
    Gopalakrishnan Loganathan
    Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    Transplantation 95:1439-47. 2013
    ..In the absence of a reliable islet potency assay, nude mice (NM) transplantation is the criterion standard to assess islet quality for clinical transplantation. There are factors other than islet quality that affect the transplant outcome...
  3. pmc A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products
    A N Balamurugan
    Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Transplantation 93:693-702. 2012
    ..The ECs consisted of purified, intact or truncated class 1 (C1) and class 2 (C2) collagenases from Clostridium histolyticum (Ch), and neutral protease (NP) from Bacillus thermoproteolyticus rokko (thermolysin) or Ch (ChNP)...
  4. doi request reprint Prediction of pancreatic tissue densities by an analytical test gradient system before purification maximizes human islet recovery for islet autotransplantation/allotransplantation
    Takayuki Anazawa
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, 420 Delaware Street S E, Minneapolis, MN 55455, USA
    Transplantation 91:508-14. 2011
    ..We developed an analytical test gradient system (ATGS) for predicting pancreatic tissue densities before COBE purification to minimize islet loss during purification...
  5. doi request reprint Differences in glucose-stimulated insulin secretion in vitro of islets from human, nonhuman primate, and porcine origin
    Kate R Mueller
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN, USA
    Xenotransplantation 20:75-81. 2013
    ..Porcine islet xenotransplantation to humans may require significantly higher dosing given the lower GSIS of AP islets compared to human islets...
  6. doi request reprint Supplements in human islet culture: human serum albumin is inferior to fetal bovine serum
    Efstathios S Avgoustiniatos
    Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    Cell Transplant 21:2805-14. 2012
    ..05). We conclude that the quality of clinical islet preparations can be improved when culture is performed in media supplemented with serum instead of albumin...
  7. doi request reprint Real-time assessment of encapsulated neonatal porcine islets prior to clinical xenotransplantation
    Jennifer P Kitzmann
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN, USA
    Xenotransplantation 19:333-6. 2012
    ..We applied the validated assay for oxygen consumption rate normalized for DNA content (OCR/DNA) to characterize ENPI viability...
  8. pmc Persufflation (or gaseous oxygen perfusion) as a method of organ preservation
    Thomas M Suszynski
    Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    Cryobiology 64:125-43. 2012
    ..Finally, key issues that need to be addressed before PSF becomes a more widely utilized preservation strategy are summarized and discussed...
  9. pmc Insulin degradation by acinar cell proteases creates a dysfunctional environment for human islets before/after transplantation: benefits of α-1 antitrypsin treatment
    Gopalakrishnan Loganathan
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Transplantation 92:1222-30. 2011
    ....
  10. doi request reprint Successful human islet isolation and transplantation indicating the importance of class 1 collagenase and collagen degradation activity assay
    A N Balamurugan
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN, USA
    Transplantation 89:954-61. 2010
    ..Here, we show the differences between the C1 collagenase found in purified collagenase products manufactured by three suppliers and the impact of differences in C1 between two suppliers on human islet yield...
  11. doi request reprint Species incompatibilities in the pig-to-macaque islet xenotransplant model affect transplant outcome: a comparison with allotransplantation
    Melanie L Graham
    Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    Xenotransplantation 18:328-42. 2011
    ..We evaluated data from our group regarding human and non-human primate (NHP) allotransplantation and pig-to-NHP xenotransplantation to identify incompatibilities in metabolic factors and their consequences for transplant outcomes...
  12. doi request reprint Microbiological safety of porcine islets: comparison with source pig
    Juan E Abrahante
    Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN 55108, USA
    Xenotransplantation 18:88-93. 2011
    ..As an islet product is isolated from the pancreas and then subjected to culture before implantation, the question is raised whether islets could be negative even if the animal itself is positive for a distinct pathogen...
  13. pmc Binding of the fibronectin-mimetic peptide, PR_b, to alpha5beta1 on pig islet cells increases fibronectin production and facilitates internalization of PR_b functionalized liposomes
    Nicole A Atchison
    Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Langmuir 26:14081-8. 2010
    ..In addition, PR_b peptide-amphiphile functionalized liposomes may be used for targeted delivery of different agents to pig islet cells...
  14. doi request reprint Paramagnetic microparticles do not elicit islet cytotoxicity with co-culture or host immune reactivity after implantation
    Thomas M Suszynski
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN 55401, USA
    Xenotransplantation 18:239-44. 2011
    ..We assessed whether MPs affect islet function or induce an adverse effect following implantation...
  15. pmc Islet assessment for transplantation
    Klearchos K Papas
    Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, 55455, USA
    Curr Opin Organ Transplant 14:674-82. 2009
    ..Development, testing, and validation of such assays have been the subject of intense investigation for the last decade. These efforts are reviewed, highlighting the most recent results while focusing on the most promising assays...
  16. doi request reprint International workshop: islet transplantation without borders enabling islet transplantation in Greece with international collaboration and innovative technology
    Klearchos K Papas
    Department of Surgery, Institute for Cellular Transplantation, University of Arizona, Tucson, AZ, USA
    Clin Transplant 27:E116-25. 2013
    ..This manuscript presents a summary of the workshop...
  17. pmc Pig pancreas anatomy: implications for pancreas procurement, preservation, and islet isolation
    Joana Ferrer
    Department of Surgery, Diabetes Institute for Immunology and Transplantation, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Transplantation 86:1503-10. 2008
    ..The primary aim of this study was to describe the vascular and ductal anatomy of the porcine pancreas to guide and improve organ preservation and enzyme perfusion...