Research Topics
Species | Sheng PanSummaryAffiliation: University of Washington Country: USA Publications
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Detail Information
Publications
Mass spectrometry based glycoproteomics--from a proteomics perspectiveSheng Pan
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Mol Cell Proteomics 10:R110.003251. 2011..This report provides an overview of the recent advances in mass spectrometry based glycoproteomic methods and technology, in the context of biomarker discovery and clinical application...- Mass spectrometry based targeted protein quantification: methods and applicationsSheng Pan
Department of Pathology, University of Washington, Seattle, Washington 98195, USA
J Proteome Res 8:787-97. 2009..This report reviews recently developed platform technologies for emerging applications of clinical proteomics and biomarker development... 
Application of targeted quantitative proteomics analysis in human cerebrospinal fluid using a liquid chromatography matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometer (LC MALDI TOF/TOF) platformSheng Pan
Department of Pathology, University of Washington, Seattle, Washington 98195, USA
J Proteome Res 7:720-30. 2008....- Multiplex targeted proteomic assay for biomarker detection in plasma: a pancreatic cancer biomarker case studySheng Pan
Department of Medicine, University of Washington, Seattle, Washington 98195, United States
J Proteome Res 11:1937-48. 2012..This information addresses important technical issues that could aid in the adoption of the targeted proteomics platform for practical clinical utility... - Protein alterations associated with pancreatic cancer and chronic pancreatitis found in human plasma using global quantitative proteomics profilingSheng Pan
Department of Medicine, University of Washington, Seattle, Washington 98195, United States
J Proteome Res 10:2359-76. 2011..The protein candidates identified in this study provide a biomarker candidate pool for future investigations... - Proteomics portrait of archival lesions of chronic pancreatitisSheng Pan
Department of Medicine, University of Washington, Seattle, Washington, United States of America
PLoS ONE 6:e27574. 2011.... 
Quantitative proteomics analysis reveals that proteins differentially expressed in chronic pancreatitis are also frequently involved in pancreatic cancerRu Chen
Division of Gastroenterology, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
Mol Cell Proteomics 6:1331-42. 2007..Altogether our study provides some insights into the molecular events in chronic pancreatitis that may lead to diverse strategies for diagnosis and treatment of these diseases...- Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasiaRu Chen
GI Division Department of Medicine, University of Washington, Seattle, WA 98195 USA
Mol Cancer 9:149. 2010..We applied quantitative proteomics to identify aberrantly elevated proteins in pancreatic juice samples derived from patients with PanIN3... - A combined dataset of human cerebrospinal fluid proteins identified by multi-dimensional chromatography and tandem mass spectrometrySheng Pan
Department of Pathology, University of Washington, Seattle, WA 98104, USA
Proteomics 7:469-73. 2007..In total, 2594 proteins were identified in well-characterized pooled human CSF samples using stringent proteomics criteria. This report summarizes our efforts to comprehensively characterize the human CSF proteome to date... - Comparison of pancreas juice proteins from cancer versus pancreatitis using quantitative proteomic analysisRu Chen
Division of Gastroenterology Department of Medicine, University of Washington, Seattle, WA, USA
Pancreas 34:70-9. 2007..Biomarkers present in pancreatic cancer frequently occur in the setting of pancreatitis. The efforts to develop diagnostic biomarkers for pancreatic cancer have thus been complicated by the false-positive involvement of pancreatitis... - Investigating neoplastic progression of ulcerative colitis with label-free comparative proteomicsDamon May
Fred Hutchinson Cancer Research Center, Molecular Diagnostics Program, Seattle, Washington 98109, USA
J Proteome Res 10:200-9. 2011.... - Quantitative proteomic profiling of pancreatic cancer juiceRu Chen
GI Division/Department of Medicine, University of Washington, Seattle, WA 98195, USA
Proteomics 6:3871-9. 2006..One of the identified proteins, insulin-like growth factor binding protein-2 was further validated by Western blotting to be elevated in pancreatic cancer juice and overexpressed in pancreatic cancer tissue... - Quantitative proteomics investigation of pancreatic intraepithelial neoplasiaSheng Pan
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Electrophoresis 30:1132-44. 2009..The dysregulated proteins identified in this study may assist in the selection of candidates for future development of biomarkers for detecting early and curable pancreatic neoplasia... - Proteomics identification of proteins in human cortex using multidimensional separations and MALDI tandem mass spectrometerSheng Pan
Department of Pathology, University of Washington School of Medicine, Seattle, Washington 98104, USA
Mol Cell Proteomics 6:1818-23. 2007..Finally 140 proteins found in the cortical proteome were present in the proteome of cerebrospinal fluid, providing tissue-specific candidates for biomarker discovery in body fluid... - Identification of glycoproteins in human cerebrospinal fluid with a complementary proteomic approachSheng Pan
Department of Pathology, University of Washington, Seattle, Washington 98104, USA
J Proteome Res 5:2769-79. 2006..This investigation, for the first time, not only categorized many glycoproteins in human CSF but also expanded the existing overall CSF protein database... - Pancreatic cancer proteome: the proteins that underlie invasion, metastasis, and immunologic escapeRu Chen
Department of Medicine, University of Washington, Seattle, 98195, USA
Gastroenterology 129:1187-97. 2005..CONCLUSIONS: Our study is the first application of isotope-coded affinity tag technology for proteomic analysis of human cancer tissue and has shown the value of this technology in identifying differentially expressed proteins in cancer... - Glycoproteomics in neurodegenerative diseasesHyejin Hwang
Department of Pathology, University of Washington, Seattle, WA 98104, USA
Mass Spectrom Rev 29:79-125. 2010.... - Stromal galectin-1 expression is associated with long-term survival in resectable pancreatic ductal adenocarcinomaRu Chen
Department of Medicine, University of Washington, Seattle, WA, USA
Cancer Biol Ther 13:899-907. 2012.... - Arousal of cancer-associated stroma: overexpression of palladin activates fibroblasts to promote tumor invasionTeresa A Brentnall
GI Division, Department of Medicine, University of Washington, Seattle, Washington, United States of America
PLoS ONE 7:e30219. 2012..Using a pancreatic cancer model, we investigated the functional consequence of overexpression of exogenous palladin in normal fibroblasts in vitro and its effect on the early stages of tumor invasion... - High throughput proteome screening for biomarker detectionSheng Pan
Institute for Systems Biology, Seattle, WA 98103, USA
Mol Cell Proteomics 4:182-90. 2005.... - Pilot study of blood biomarker candidates for detection of pancreatic cancerRu Chen
GI Division, Department of Medicine, University of Washington, Seattle, WA 98195, USA
Pancreas 39:981-8. 2010..Biomarkers that detect pancreatic cancer at earlier stages could improve the outcome of this deadly disease... - ProbIDtree: an automated software program capable of identifying multiple peptides from a single collision-induced dissociation spectrum collected by a tandem mass spectrometerNing Zhang
The Institute for Systems Biology, Seattle, WA 98103 8904, USA
Proteomics 5:4096-106. 2005..The results using MALDI-TOF-TOF MS/MS data demonstrate that multiple peptides can be effectively identified simultaneously with high confidence using ProbIDtree... - Large-scale quantitative glycoproteomics analysis of site-specific glycosylation occupancySheng Pan
Department of Medicine, University of Washington, Seattle, WA 98195, USA
Mol Biosyst 8:2850-6. 2012..We further demonstrated this approach by quantitatively characterizing the N-glycoproteome of human pancreas... - Identification of proteins in human substantia nigraEfstathia Kitsou
Department of Pathology, University of Washington School of Medicine Seattle, Seattle WA, USA
Proteomics Clin Appl 2:776-82. 2008..Further characterization of the SNpc proteome will significantly facilitate our understanding of the function and expression of proteins involved in PD, as well as provide potential proteins that may be utilized as biomarkers... - Quantitative proteomics analysis integrated with microarray data reveals that extracellular matrix proteins, catenins, and p53 binding protein 1 are important for chemotherapy response in ovarian cancersSheng Pan
Department of Pathology, University of Washington, Seattle, Washington, USA
OMICS 13:345-54. 2009..Our results suggest that chemotherapy response may be determined by multiple and complex system properties involving extracellular-matrix, cell adhesion and junction proteins... - Proteomic profiling of pancreatic cancer for biomarker discoveryRu Chen
GI Division/Department of Medicine, University of Washington, Seattle, WA 98195, USA
Mol Cell Proteomics 4:523-33. 2005..In this article, we discuss recent progress and challenges for applying quantitative proteomics technologies for biomarker discovery in pancreatic cancer... - Using 'omics' to define pathogenesis and biomarkers of Parkinson's diseaseW Michael Caudle
University of Washington, Seattle, WA, USA
Expert Rev Neurother 10:925-42. 2010..It is hoped that further integration of these techniques will yield a more comprehensive understanding of Parkinson's disease etiology and the biological pathways that mediate neurodegeneration... - Proteomic identification of proteins in the human brain: Towards a more comprehensive understanding of neurodegenerative diseaseW Michael Caudle
Department of Pathology, University of Washington School of Medicine, Seattle, WA, USA
Proteomics Clin Appl 2:1484-97. 2008.... - Quantitative proteomics by stable isotope labeling and mass spectrometrySheng Pan
Institute for System Biology, Seattle, WA, USA
Methods Mol Biol 367:209-18. 2007....