Sheng Pan

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. doi request reprint Application of targeted quantitative proteomics analysis in human cerebrospinal fluid using a liquid chromatography matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometer (LC MALDI TOF/TOF) platform
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    J Proteome Res 7:720-30. 2008
  2. pmc Mass spectrometry based glycoproteomics--from a proteomics perspective
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, WA 98195, USA
    Mol Cell Proteomics 10:R110.003251. 2011
  3. pmc Mass spectrometry based targeted protein quantification: methods and applications
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    J Proteome Res 8:787-97. 2009
  4. pmc Multiplex targeted proteomic assay for biomarker detection in plasma: a pancreatic cancer biomarker case study
    Sheng Pan
    Department of Medicine, University of Washington, Seattle, Washington 98195, United States
    J Proteome Res 11:1937-48. 2012
  5. pmc Protein alterations associated with pancreatic cancer and chronic pancreatitis found in human plasma using global quantitative proteomics profiling
    Sheng Pan
    Department of Medicine, University of Washington, Seattle, Washington 98195, United States
    J Proteome Res 10:2359-76. 2011
  6. ncbi request reprint Quantitative proteomics analysis reveals that proteins differentially expressed in chronic pancreatitis are also frequently involved in pancreatic cancer
    Ru Chen
    Division of Gastroenterology, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Proteomics 6:1331-42. 2007
  7. pmc Proteomics portrait of archival lesions of chronic pancreatitis
    Sheng Pan
    Department of Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 6:e27574. 2011
  8. pmc Large-scale quantitative glycoproteomics analysis of site-specific glycosylation occupancy
    Sheng Pan
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Mol Biosyst 8:2850-6. 2012
  9. pmc Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia
    Ru Chen
    GI Division Department of Medicine, University of Washington, Seattle, WA 98195 USA
    Mol Cancer 9:149. 2010
  10. ncbi request reprint A combined dataset of human cerebrospinal fluid proteins identified by multi-dimensional chromatography and tandem mass spectrometry
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, WA 98104, USA
    Proteomics 7:469-73. 2007

Collaborators

Detail Information

Publications31

  1. doi request reprint Application of targeted quantitative proteomics analysis in human cerebrospinal fluid using a liquid chromatography matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometer (LC MALDI TOF/TOF) platform
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    J Proteome Res 7:720-30. 2008
    ....
  2. pmc Mass spectrometry based glycoproteomics--from a proteomics perspective
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, WA 98195, USA
    Mol Cell Proteomics 10:R110.003251. 2011
    ..This report provides an overview of the recent advances in mass spectrometry based glycoproteomic methods and technology, in the context of biomarker discovery and clinical application...
  3. pmc Mass spectrometry based targeted protein quantification: methods and applications
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    J Proteome Res 8:787-97. 2009
    ..This report reviews recently developed platform technologies for emerging applications of clinical proteomics and biomarker development...
  4. pmc Multiplex targeted proteomic assay for biomarker detection in plasma: a pancreatic cancer biomarker case study
    Sheng Pan
    Department of Medicine, University of Washington, Seattle, Washington 98195, United States
    J Proteome Res 11:1937-48. 2012
    ..This information addresses important technical issues that could aid in the adoption of the targeted proteomics platform for practical clinical utility...
  5. pmc Protein alterations associated with pancreatic cancer and chronic pancreatitis found in human plasma using global quantitative proteomics profiling
    Sheng Pan
    Department of Medicine, University of Washington, Seattle, Washington 98195, United States
    J Proteome Res 10:2359-76. 2011
    ..The protein candidates identified in this study provide a biomarker candidate pool for future investigations...
  6. ncbi request reprint Quantitative proteomics analysis reveals that proteins differentially expressed in chronic pancreatitis are also frequently involved in pancreatic cancer
    Ru Chen
    Division of Gastroenterology, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Proteomics 6:1331-42. 2007
    ..Altogether our study provides some insights into the molecular events in chronic pancreatitis that may lead to diverse strategies for diagnosis and treatment of these diseases...
  7. pmc Proteomics portrait of archival lesions of chronic pancreatitis
    Sheng Pan
    Department of Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 6:e27574. 2011
    ....
  8. pmc Large-scale quantitative glycoproteomics analysis of site-specific glycosylation occupancy
    Sheng Pan
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Mol Biosyst 8:2850-6. 2012
    ..We further demonstrated this approach by quantitatively characterizing the N-glycoproteome of human pancreas...
  9. pmc Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia
    Ru Chen
    GI Division Department of Medicine, University of Washington, Seattle, WA 98195 USA
    Mol Cancer 9:149. 2010
    ..We applied quantitative proteomics to identify aberrantly elevated proteins in pancreatic juice samples derived from patients with PanIN3...
  10. ncbi request reprint A combined dataset of human cerebrospinal fluid proteins identified by multi-dimensional chromatography and tandem mass spectrometry
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, WA 98104, USA
    Proteomics 7:469-73. 2007
    ..In total, 2594 proteins were identified in well-characterized pooled human CSF samples using stringent proteomics criteria. This report summarizes our efforts to comprehensively characterize the human CSF proteome to date...
  11. pmc Comparison of pancreas juice proteins from cancer versus pancreatitis using quantitative proteomic analysis
    Ru Chen
    Division of Gastroenterology Department of Medicine, University of Washington, Seattle, WA, USA
    Pancreas 34:70-9. 2007
    ..Biomarkers present in pancreatic cancer frequently occur in the setting of pancreatitis. The efforts to develop diagnostic biomarkers for pancreatic cancer have thus been complicated by the false-positive involvement of pancreatitis...
  12. pmc Investigating neoplastic progression of ulcerative colitis with label-free comparative proteomics
    Damon May
    Fred Hutchinson Cancer Research Center, Molecular Diagnostics Program, Seattle, Washington 98109, USA
    J Proteome Res 10:200-9. 2011
    ....
  13. ncbi request reprint Quantitative proteomic profiling of pancreatic cancer juice
    Ru Chen
    GI Division Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Proteomics 6:3871-9. 2006
    ..One of the identified proteins, insulin-like growth factor binding protein-2 was further validated by Western blotting to be elevated in pancreatic cancer juice and overexpressed in pancreatic cancer tissue...
  14. pmc Quantitative proteomics investigation of pancreatic intraepithelial neoplasia
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, WA 98195, USA
    Electrophoresis 30:1132-44. 2009
    ..The dysregulated proteins identified in this study may assist in the selection of candidates for future development of biomarkers for detecting early and curable pancreatic neoplasia...
  15. ncbi request reprint Proteomics identification of proteins in human cortex using multidimensional separations and MALDI tandem mass spectrometer
    Sheng Pan
    Department of Pathology, University of Washington School of Medicine, Seattle, Washington 98104, USA
    Mol Cell Proteomics 6:1818-23. 2007
    ..Finally 140 proteins found in the cortical proteome were present in the proteome of cerebrospinal fluid, providing tissue-specific candidates for biomarker discovery in body fluid...
  16. pmc Quantitative glycoproteomics analysis reveals changes in N-glycosylation level associated with pancreatic ductal adenocarcinoma
    Sheng Pan
    The Division of Gastroenterology, Department of Medicine, University of Washington, 1959 North East Pacific Street, Seattle, Washington 98195, United States
    J Proteome Res 13:1293-306. 2014
    ....
  17. pmc Glycoproteomics in neurodegenerative diseases
    Hyejin Hwang
    Department of Pathology, University of Washington, Seattle, WA 98104, USA
    Mass Spectrom Rev 29:79-125. 2010
    ....
  18. ncbi request reprint Identification of glycoproteins in human cerebrospinal fluid with a complementary proteomic approach
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington 98104, USA
    J Proteome Res 5:2769-79. 2006
    ..This investigation, for the first time, not only categorized many glycoproteins in human CSF but also expanded the existing overall CSF protein database...
  19. pmc Stromal galectin-1 expression is associated with long-term survival in resectable pancreatic ductal adenocarcinoma
    Ru Chen
    Department of Medicine, University of Washington, Seattle, WA, USA
    Cancer Biol Ther 13:899-907. 2012
    ....
  20. pmc Arousal of cancer-associated stroma: overexpression of palladin activates fibroblasts to promote tumor invasion
    Teresa A Brentnall
    GI Division, Department of Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 7:e30219. 2012
    ..Using a pancreatic cancer model, we investigated the functional consequence of overexpression of exogenous palladin in normal fibroblasts in vitro and its effect on the early stages of tumor invasion...
  21. pmc Pilot study of blood biomarker candidates for detection of pancreatic cancer
    Ru Chen
    GI Division, Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Pancreas 39:981-8. 2010
    ..Biomarkers that detect pancreatic cancer at earlier stages could improve the outcome of this deadly disease...
  22. ncbi request reprint High throughput proteome screening for biomarker detection
    Sheng Pan
    Institute for Systems Biology, Seattle, WA 98103, USA
    Mol Cell Proteomics 4:182-90. 2005
    ....
  23. ncbi request reprint ProbIDtree: an automated software program capable of identifying multiple peptides from a single collision-induced dissociation spectrum collected by a tandem mass spectrometer
    Ning Zhang
    The Institute for Systems Biology, Seattle, WA 98103 8904, USA
    Proteomics 5:4096-106. 2005
    ..The results using MALDI-TOF-TOF MS/MS data demonstrate that multiple peptides can be effectively identified simultaneously with high confidence using ProbIDtree...
  24. ncbi request reprint Pancreatic cancer proteome: the proteins that underlie invasion, metastasis, and immunologic escape
    Ru Chen
    Department of Medicine, University of Washington, Seattle, 98195, USA
    Gastroenterology 129:1187-97. 2005
    ....
  25. pmc Tissue proteomics in pancreatic cancer study: discovery, emerging technologies, and challenges
    Sheng Pan
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Proteomics 13:710-21. 2013
    ..Here, we provide an overview on the tissue proteomics studies of pancreatic cancer reported in the past few years in light of discovery and technology development...
  26. doi request reprint Identification of proteins in human substantia nigra
    Efstathia Kitsou
    Department of Pathology, University of Washington School of Medicine Seattle, Seattle WA, USA
    Proteomics Clin Appl 2:776-82. 2008
    ..Further characterization of the SNpc proteome will significantly facilitate our understanding of the function and expression of proteins involved in PD, as well as provide potential proteins that may be utilized as biomarkers...
  27. pmc Using 'omics' to define pathogenesis and biomarkers of Parkinson's disease
    W Michael Caudle
    University of Washington, Seattle, WA, USA
    Expert Rev Neurother 10:925-42. 2010
    ..It is hoped that further integration of these techniques will yield a more comprehensive understanding of Parkinson's disease etiology and the biological pathways that mediate neurodegeneration...
  28. ncbi request reprint Proteomic profiling of pancreatic cancer for biomarker discovery
    Ru Chen
    GI Division Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Mol Cell Proteomics 4:523-33. 2005
    ..In this article, we discuss recent progress and challenges for applying quantitative proteomics technologies for biomarker discovery in pancreatic cancer...
  29. doi request reprint Quantitative proteomics analysis integrated with microarray data reveals that extracellular matrix proteins, catenins, and p53 binding protein 1 are important for chemotherapy response in ovarian cancers
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington, USA
    OMICS 13:345-54. 2009
    ..Our results suggest that chemotherapy response may be determined by multiple and complex system properties involving extracellular-matrix, cell adhesion and junction proteins...
  30. pmc Proteomic identification of proteins in the human brain: Towards a more comprehensive understanding of neurodegenerative disease
    W Michael Caudle
    Department of Pathology, University of Washington School of Medicine, Seattle, WA, USA
    Proteomics Clin Appl 2:1484-97. 2008
    ....
  31. ncbi request reprint Quantitative proteomics by stable isotope labeling and mass spectrometry
    Sheng Pan
    Institute for System Biology, Seattle, WA, USA
    Methods Mol Biol 367:209-18. 2007
    ....