Genomes and Genes
Affiliation: University of Rochester
- Primitive erythropoiesis in the mammalian embryoJames Palis
University of Rochester Medical Center, Department of Pediatrics, Center for Pediatric Biomedical Research, New York 14642, USA
Int J Dev Biol 54:1011-8. 2010..This surprising finding indicates that the primitive erythroid lineage is indeed mammalian, rather than non-mammalian, in character...
- Erythropoietin critically regulates the terminal maturation of murine and human primitive erythroblastsJeffrey Malik
Haematologica 98:1778-87. 2013..We conclude that erythropoietin plays an evolutionarily conserved role in promoting the proliferation, survival, and appropriate timing of terminal maturation of primitive erythroid precursors. ..
- Stat and interferon genes identified by network analysis differentially regulate primitive and definitive erythropoiesisEmily Greenfest-Allen
Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA stoec
BMC Syst Biol 7:38. 2013..Inferred networks are strongly connected and do not fit a scale-free model, making it difficult to identify essential regulators using the hub-essentiality standard...
- Yolk-sac hematopoiesis: the first blood cells of mouse and manJ Palis
University of Rochester, Department of Pediatrics and Cancer Center, Rochester, NY 14642, USA
Exp Hematol 29:927-36. 2001..To review the process of blood-cell formation in the murine and human yolk sac...
- Ontogeny of erythropoiesisJames Palis
Department of Pediatrics, University of Rochester Medical Center, Rochester, New York 14642, USA
Curr Opin Hematol 15:155-61. 2008....
- Erythropoietin-receptor expression and function during the initiation of murine yolk sac erythropoiesisJ K McGann
Department of Pediatrics, University of Rochester Medical Center, NY 14642, USA
Exp Hematol 25:1149-57. 1997..These results also suggest that other growth factor signaling cascades are active during the onset of mammalian erythropoiesis...
- Development of erythroid and myeloid progenitors in the yolk sac and embryo proper of the mouseJ Palis
University of Rochester Medical Center, Department of Pediatrics and Cancer Center, Box 777, Rochester, NY 14642, USA rochester edu
Development 126:5073-84. 1999....
- Initiation of murine embryonic erythropoiesis: a spatial analysisL Silver
University of Rochester Medical Center, Department of Pediatrics and Cancer Center, NY 14642, USA
Blood 89:1154-64. 1997..During the stages analyzed, tal-1 and rbtn2 expression was also present in posterior embryonic mesoderm, while GATA-1 and GATA-2 expression was evident in extraembryonic tissues of ectodermal origin...
- Embryonic expression and function of the chemokine SDF-1 and its receptor, CXCR4K E McGrath
Department of Pediatrics and Cancer Center, University of Rochester Medical Center, Rochester, New York, 14642, USA
Dev Biol 213:442-56. 1999..Migration occurred with an optimum dose similar to that found for adult hematopoietic cells and was dependent on the presence of SDF-1 in a gradient. This work suggests roles for chemokine signaling in multiple embryogenic events...
- Subtractive hybridization reveals tissue-specific expression of ahnak during embryonic developmentP D Kingsley
University of Rochester Medical Center, Department of Pediatrics and Cancer Center, 601 Elmwood Avenue, Rochester, New York 14642, USA
Dev Growth Differ 43:133-43. 2001..The potential functions of ahnak are discussed in light of these results...
- The relationship of CD5+ B lymphocytes to macrophages: insights from normal biphenotypic B/macrophage cellsM A Borrello
Vaccinex, Laboratory of Cellular Immunology, Rochester, NY, USA
Int Rev Immunol 20:137-55. 2001..This review considers normal B/macrophage cell function in an evolutionary context where a primitive, flexible cell type could perform dual roles in adaptive and innate immunity...
- Expression of homeobox genes, including an insulin promoting factor, in the murine yolk sac at the time of hematopoietic initiationK E McGrath
Department of Pediatrics, University of Rochester Medical Center, NY 14642, USA
Mol Reprod Dev 48:145-53. 1997..We hypothesize the early expression of insulin in the YS is required for the expansion of insulin responsive cells including primitive erythroblasts...
- "Maturational" globin switching in primary primitive erythroid cellsPaul D Kingsley
Department of Pediatrics, University of Rochester Medical Center, Center for Pediatric Biomedical Research, Box 703, 601 Elmwood Ave, Rochester, NY 14642, USA
Blood 107:1665-72. 2006..Globin switching during ontogeny is due not only to the sequential appearance of primitive and definitive lineages but also to changes in globin expression as primitive erythroblasts mature in the bloodstream...
- Yolk sac-derived primitive erythroblasts enucleate during mammalian embryogenesisPaul D Kingsley
Department of Pediatrics, Center for Human Genetics and Molecular Pediatric Disease, University of Rochester Medical Center, NY 14642, USA
Blood 104:19-25. 2004..Our studies establish a new paradigm in the understanding of primitive erythropoiesis and support the concept that primitive erythropoiesis in mice shares many similarities with definitive erythropoiesis of mammals...
- Spatial and temporal emergence of high proliferative potential hematopoietic precursors during murine embryogenesisJ Palis
University of Rochester Medical Center, Department of Pediatrics and Cancer Center, Rochester, NY 14642, USA
Proc Natl Acad Sci U S A 98:4528-33. 2001..Our findings support the hypothesis that definitive but not primitive hematopoietic progenitors originate from yolk sac-derived HPP-CFC during late gastrulation...
- Developmental expression of extracellular glutathione peroxidase suggests antioxidant roles in deciduum, visceral yolk sac, and skinP D Kingsley
Department of Pediatrics and Cancer Center, University of Rochester Medical Center, New York 14642, USA
Mol Reprod Dev 49:343-55. 1998..These findings suggest that EGPx may serve a wider antioxidant role than previously recognized in the interstitium of multiple localized tissues, particularly those associated with the active transport of lipids...
- Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetusKathleen E McGrath
University of Rochester Medical Center, Department of Pediatrics, Center for Pediatric Biomedical Research, Rochester, NY 14642, USA
Blood 111:2409-17. 2008..Continued studies comparing primitive and definitive erythropoiesis will lead to an improved understanding of terminal erythroid maturation...
- Multispectral imaging of hematopoietic cells: where flow meets morphologyKathleen E McGrath
University of Rochester Medical Center, Department of Pediatrics, Center for Pediatric Biomedical Research, Box 703, 601 Elmwood Ave, Rochester, NY 14642, United States
J Immunol Methods 336:91-7. 2008....
- Histone hyperacetylation within the beta-globin locus is context-dependent and precedes high-level gene expressionGeorge Fromm
Departments of Pediatrics, Center for Pediatric Biomedical Research, University of Rochester Medical Center, Box 703, 601 Elmwood Ave, Rochester, NY14642, USA
Blood 114:3479-88. 2009..These data narrow the range of possible mechanisms by which hyperacetylated domains form...
- Circulation is established in a stepwise pattern in the mammalian embryoKathleen E McGrath
Center for Human Genetics and Molecular Pediatric Diseases, Department of Pediatrics, University of Rochester, NY, USA
Blood 101:1669-76. 2003..We conclude that a functional vascular system develops gradually and that specialized vascular-hematopoietic environments exist after circulation becomes fully established...
- All primitive and definitive hematopoietic progenitor cells emerging before E10 in the mouse embryo are products of the yolk sacChristopher T Lux
Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202 5254, USA
Blood 111:3435-8. 2008..Thus, primitive erythroblasts and essentially all definitive HPCs destined to initially seed the fetal liver after E9.5 are generated in the YS between E7.0-E9.5 and are redistributed into the EP via the systemic circulation...
- Sox6 directly silences epsilon globin expression in definitive erythropoiesisZanhua Yi
Department of Pediatrics, University of Arizona, College of Medicine, Tucson, Arizona, USA
PLoS Genet 2:e14. 2006..Thus, Sox6 regulation of epsilony globin might provide a novel therapeutical target in the treatment of hemoglobinopathies such as sickle cell anemia and thalassemia...
- Differential gene expression during early murine yolk sac developmentJ Palis
University of Rochester, Department of Pediatrics, New York, USA
Mol Reprod Dev 42:19-27. 1995..These results are consistent with the hypothesis that at E7.5, the yolk sac endoderm provides differentiated liver-like functions, while the newly developing extraembryonic mesoderm is still a largely undifferentiated tissue...
- "Liver function tests" are not always tests of liver functionD N Korones
Department of Pediatrics, University of Rochester, School of Medicine and Dentistry, Children s Hospital at Strong, New York 14642, USA
Am J Hematol 66:46-8. 2001..Hematologists/oncologists should consider obtaining a serum CK to rule out muscle disease in patients with unexplained elevations of AST, ALT, and LDH...
- Hematopoiesis in the yolk sac: more than meets the eyeKathleen E McGrath
Department of Pediatrics and the Center for Pediatric Biomedical Research, University of Rochester Medical Center, Rochester, NY 14642, USA
Exp Hematol 33:1021-8. 2005....
- Primitive erythropoiesis and megakaryopoiesis in the yolk sac are independent of c-mybJoanna Tober
Center for Pediatric Biomedical Research, Department of Pediatrics, University of Rochester Medical Center, Rochester, NY 14642, USA
Blood 111:2636-9. 2008..5 yolk sac and E11.5 liver. Despite this impaired megakaryocyte potential, c-myb-null fetuses had normal platelet numbers at E12.5 but became thrombocytopenic by E15.5, suggesting that c-myb is required for sustained thrombopoiesis...
- The megakaryocyte lineage originates from hemangioblast precursors and is an integral component both of primitive and of definitive hematopoiesisJoanna Tober
Center for Pediatric Biomedical Research, Department of Pediatrics, University of Rochester Medical Center, NY 14642, USA
Blood 109:1433-41. 2007....
- Analysis of hematopoietic progenitors in the mouse embryoJames Palis
Department of Pediatrics, Center for Genetics and Molecular Pediatric Disease, University of Rochester Medical Center, Rochester, NY, USA
Methods Mol Med 105:289-302. 2005..This chapter describes the methodologies for the culture and identification of unipotential and multipotential hematopoietic progenitors in the mouse embryo...
- Ontogeny of erythropoiesis in the mammalian embryoKathleen McGrath
Department of Pediatrics, Center for Pediatric Biomedical Research, University of Rochester, Rochester, New York 14642, USA
Curr Top Dev Biol 82:1-22. 2008..Continued comparative studies of primitive and definitive erythropoiesis in mammalian and nonmammalian embryos will lead to an improved understanding of terminal erythroid maturation and globin gene regulation...
- Reticulocyte and micronucleated reticulocyte responses to gamma irradiation: dose-response and time-course profiles measured by flow cytometryStephen D Dertinger
Litron Laboratories, Rochester, NY 14623, USA
Mutat Res 634:119-25. 2007....
- Transient glutathione depletion determines terminal differentiation in HL-60 cellsSuzanne M Krance
University of Rochester School of Medicine, Rochester, New York, USA
Oxid Med Cell Longev 3:53-60. 2010..These findings indicate that intracellular GSH levels are regulated in a complex fashion during HL-60 cell differentiation, and that transient GSH depletion using low doses of CDNB and DEM enhances the differentiation process...
- Pig-a mutation: kinetics in rat erythrocytes following exposure to five prototypical mutagensSouk Phonethepswath
Litron Laboratories, Rochester, New York 14623, USA
Toxicol Sci 114:59-70. 2010..The kinetics and ease of scoring this blood-based endpoint suggest that integration into routine toxicology studies will be feasible...
- Foxp3 regulates megakaryopoiesis and platelet functionJamie J Bernard
Department of Environmental Medicine, University of Rochester, Rochester, NY 14642, USA
Arterioscler Thromb Vasc Biol 29:1874-82. 2009..Scurfy mice and some humans with IPEX are also thrombocytopenic. The purpose of this study was to determine whether the absence of functional Foxp3 leads to defects in megakaryocytes and platelets...
- Haemangioblast commitment is initiated in the primitive streak of the mouse embryoTara L Huber
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York City, New York 10029, USA
Nature 432:625-30. 2004....
- A heme export protein is required for red blood cell differentiation and iron homeostasisSiobán B Keel
Division of Hematology, University of Washington, Seattle, WA 98195, USA
Science 319:825-8. 2008..Thus, the trafficking of heme, and not just elemental iron, facilitates erythropoiesis and systemic iron balance...
- Developmental biology: no red cell is an islandJames Palis
Nature 432:964-5. 2004
- Onset of cardiac function during early mouse embryogenesis coincides with entry of primitive erythroblasts into the embryo properRui Ping Ji
Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY, USA
Circ Res 92:133-5. 2003..In situ hybridization revealed an onset of the heartbeat coincident with the appearance of yolk sac-derived erythroblasts in the embryo proper at 5 S. Early maturation of the circulation follows a tightly coordinated program...
- Lineage analysis of the hemangioblast as defined by FLK1 and SCL expressionYun Shin Chung
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA
Development 129:5511-20. 2002..Replating studies demonstrate that hematopoietic progenitors are enriched within FLK1(+)CD4(+) and FLK1(-)CD4(+) cells, while endothelial cells develop from FLK1(+)CD4(+) and FLK1(+)CD4(-) cell populations...
- Runx1 is essential for hematopoietic commitment at the hemangioblast stage of development in vitroGeorges Lacaud
Carl C Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY, USA
Blood 100:458-66. 2002....
- The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesisDavid G Ransom
Division of Hematology Oncology, Children s Hospital and Harvard Medical School, Boston, Massachusetts, USA
PLoS Biol 2:E237. 2004..Our results establish a major role for this transcriptional intermediary factor in the differentiation of hematopoietic cells in vertebrates...
- Deficiency of glutaredoxin 5 reveals Fe-S clusters are required for vertebrate haem synthesisRebecca A Wingert
Stem Cell Program and Division Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 436:1035-39. 2005..These findings uncover a connection between haem biosynthesis and Fe-S clusters, indicating that haemoglobin production in the differentiating red cell is regulated through Fe-S cluster assembly...
- cdx4 mutants fail to specify blood progenitors and can be rescued by multiple hox genesAlan J Davidson
Department of Medicine, Division of Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
Nature 425:300-6. 2003..Taken together, these findings demonstrate that cdx4 regulates hox genes and is necessary for the specification of haematopoietic cell fate during vertebrate embryogenesis...
- Embryonic hematopoietic stem cell origin and ontogenyJames Palis; Fiscal Year: 2004..A better understanding of the initiation of mammalian hematopoiesis will provide insights into the ontogeny, regulation and expansion of HSCs, as well as the origin of leukemias and bone marrow failure syndromes. ..
- Initiation of Mammalian Yolk Sac HematopoiesisJames Palis; Fiscal Year: 2006..This will ultimately lead to improvements in bone marrow transplantation for the curative treatment of congenital anemias, genetic diseases and several forms of childhood and adult cancers. ..
- Ontogeny of erythroid lineage differentiationJames Palis; Fiscal Year: 2010..These studies will improve our understanding of the causes of anemia and will ultimately lead to curative treatments for children and adults with sickle cell disease and thalassemia syndromes. ..