Norman PaceSummaryAffiliation: University of Colorado Country: USA Publications
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Publications
RNase P: interface of the RNA and protein worldsDonald Evans
Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Campus Box 347, Boulder, CO 80309-0347, USA
Trends Biochem Sci 31:333-41. 2006..In vitro reconstitution of activity is providing insight into the role of proteins in the RNase P holoenzyme. Together, these findings are beginning to impart an understanding of the coevolution of the RNA and protein worlds...- Gastrointestinal microbiology enters the metagenomics eraDaniel N Frank
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
Curr Opin Gastroenterol 24:4-10. 2008..This review summarizes the molecular methods of culture-independent microbiology ('metagenomics') and their recent application to studies of the human gastrointestinal tract in both health and disease... - Q and ANorman Pace
Department of Molecular, Cellular and Development Biology, University of Colarado 80309-0347, USA
Curr Biol 16:R5-R6. 2006 
Mapping the tree of life: progress and prospectsNorman R Pace
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Microbiol Mol Biol Rev 73:565-76. 2009..Obstacles to accurate inference of deep phylogenetic relationships are both systematic, in molecular phylogenetic calculations, and practical, due to a paucity of sequence representation for many groups of organisms...- Phylogenetic composition of Rocky Mountain endolithic microbial ecosystemsJeffrey J Walker
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Appl Environ Microbiol 73:3497-504. 2007..Collectively, results of this study support the idea that patterns of microbial diversity found in endolithic communities are governed by principles similar to those observed in macroecological systems... - Endolithic microbial ecosystemsJeffrey J Walker
University of Colorado at Boulder, Department of Molecular, Cellular and Developmental Biology, Boulder, Colorado 80309 0347, USA
Annu Rev Microbiol 61:331-47. 2007..Recent results also explore geobiological processes that shape the current biosphere and potentially provide clues to life's history on Earth and where to seek life elsewhere in the Solar System... 
Disease phenotype and genotype are associated with shifts in intestinal-associated microbiota in inflammatory bowel diseasesDaniel N Frank
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80045, USA
Inflamm Bowel Dis 17:179-84. 2011..We hypothesize that NOD2 and ATG16L1 risk alleles may be associated with these alterations...- Hydrogen and bioenergetics in the Yellowstone geothermal ecosystemJohn R Spear
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
Proc Natl Acad Sci U S A 102:2555-60. 2005.... - Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western AustraliaDominic Papineau
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
Appl Environ Microbiol 71:4822-32. 2005..These results provide a framework for understanding the kinds of organisms that build contemporary stromatolites, their ecology, and their relevance to stromatolites preserved in the geological record... - Unexpected diversity and complexity of the Guerrero Negro hypersaline microbial matRuth E Ley
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
Appl Environ Microbiol 72:3685-95. 2006..The sequences determined in this study have been submitted to the GenBank database and assigned accession numbers DQ 329539 to DQ 331020, and DQ 397339 to DQ 397511... - Phylogenetic diversity and ecology of environmental ArchaeaCharles E Robertson
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
Curr Opin Microbiol 8:638-42. 2005..Although it is apparent that Archaea can be found in all environments, the chemistry of their ecological context is mostly unknown... - Diversity and stratification of archaea in a hypersaline microbial matCharles E Robertson
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Appl Environ Microbiol 75:1801-10. 2009..Some clades did not segregate by depth in the mat, indicating broad metabolic repertoires, undersampling, or both... - Time for a changeNorman R Pace
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347, USA
Nature 441:289. 2006 - Complexity in natural microbial ecosystems: the Guerrero Negro experienceJohn R Spear
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, 347 UCB, Boulder, Colorado 80309, USA
Biol Bull 204:168-73. 2003..To date, analyses of these two environments indicate the ubiquitous dominance of uncultured organisms of phylogenetic kinds not generally thought to be associated with hypersaline environments... - Bacterial RNase P: a new view of an ancient enzymeAlexei V Kazantsev
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
Nat Rev Microbiol 4:729-40. 2006..The goal of the present review is to summarize what is known about the bacterial RNase P, and to bring together the recent structural results with extensive earlier biochemical and phylogenetic findings... - Geobiology of a microbial endolithic community in the Yellowstone geothermal environmentJeffrey J Walker
Department of Molecular, Cellular and Developmental Biology and the Center for Astrobiology, University of Colorado, Boulder, Colorado 80309-0347, USA
Nature 434:1011-4. 2005..Remnants of these communities could serve as biosignatures and provide important clues about ancient life associated with geothermal environments on the Earth or elsewhere in the Solar System... - New perspective on uncultured bacterial phylogenetic division OP11J Kirk Harris
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
Appl Environ Microbiol 70:845-9. 2004.... - Culture-independent microbiological analysis of foley urinary catheter biofilmsDaniel N Frank
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO, USA
PLoS ONE 4:e7811. 2009..Prevention of catheter-associated urinary tract infection (CAUTI), a leading cause of nosocomial disease, is complicated by the propensity of bacteria to form biofilms on indwelling medical devices [1,2,3,4,5]... - Opportunistic pathogens enriched in showerhead biofilmsLeah M Feazel
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Proc Natl Acad Sci U S A 106:16393-9. 2009..The health risk associated with showerhead microbiota needs investigation in persons with compromised immune or pulmonary systems... - Culture-independent molecular analysis of microbial constituents of the healthy human outer earDaniel N Frank
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
J Clin Microbiol 41:295-303. 2003..Alternatively, culture analyses of diseases of the middle ear may have been confounded by these contaminating commensal organisms... - Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseasesDaniel N Frank
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Proc Natl Acad Sci U S A 104:13780-5. 2007..Patient stratification by GI microbiota provides further evidence that CD represents a spectrum of disease states and suggests that treatment of some forms of IBD may be facilitated by redress of the detected microbiological imbalances... - High-resolution structure of RNase P protein from Thermotoga maritimaAlexei V Kazantsev
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Proc Natl Acad Sci U S A 100:7497-502. 2003..The protein is found to dimerize through an extensive, well-ordered interface. This dimerization may reflect a mechanism of thermal stability of the protein before assembly with the RNA moiety of the holoenzyme... - Eucaryotic diversity in a hypersaline microbial matLeah M Feazel
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 90309 0347, USA
Appl Environ Microbiol 74:329-32. 2008..Six eucaryotic kingdoms were represented, as well as a novel cluster of sequences. Nematode sequences dominated the clone libraries... - Molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosisJ Kirk Harris
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
Proc Natl Acad Sci U S A 104:20529-33. 2007..These results show that molecular techniques provide a broader perspective on airway bacteria than do routine clinical cultures and thus can identify targets for further clinical evaluation... - The human nasal microbiota and Staphylococcus aureus carriageDaniel N Frank
Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, Colorado, United States of America
PLoS ONE 5:e10598. 2010..aureus colonization we determined the composition and dynamics of the nasal microbiota and correlated specific microorganisms with S. aureus colonization... - Use of specific rRNA oligonucleotide probes for microscopic detection of Mycobacterium avium complex organisms in tissueAllison L St Amand
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
J Clin Microbiol 43:1505-14. 2005..intracellulare, the two M. avium subspecies associated with human disease, or all members of MAC. The results call into question the validity of ISH results derived by the use of other gene loci, such as IS900... - Culture-independent analysis of indomethacin-induced alterations in the rat gastrointestinal microbiotaAndrew B Dalby
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
Appl Environ Microbiol 72:6707-15. 2006..These data suggest that treatment of NSAID-induced ulceration may be facilitated by addressing the microbiological imbalances... - Microbial diversity in chronic open woundsDaniel N Frank
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, USA
Wound Repair Regen 17:163-72. 2009.... - Structural implications of novel diversity in eucaryal RNase P RNASteven M Marquez
Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Room A3B40, Boulder, CO 80309-0347, USA
RNA 11:739-51. 2005..The eucaryal RNA has been remodeled through evolution as a simplified version of the structure found in bacterial and archaeal RNase P RNAs... - Use of specific rRNA oligonucleotide probes for microscopic detection of Mycobacterium tuberculosis in culture and tissue specimensAllison L St Amand
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
J Clin Microbiol 43:5369-71. 2005..We report a rapid and reliable in situ hybridization technique using rRNA oligonucleotide probes for the identification of M. tuberculosis in tissues and cultures... - Structural perspective on the activation of RNAse P RNA by proteinAmy H Buck
Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA
Nat Struct Mol Biol 12:958-64. 2005..The data are consistent with a role of the protein in substrate recognition and support a new model of the holoenzyme that is based on a recently solved crystal structure of RNase P RNA... - Microbial composition of near-boiling silica-depositing thermal springs throughout Yellowstone National ParkCarrine E Blank
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA
Appl Environ Microbiol 68:5123-35. 2002..Therefore, species- or subspecies-level divergences are present but not detectable in highly conserved SSU sequences... - Comparative molecular analysis of endoevaporitic microbial communitiesJason W Sahl
Department of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401, USA
Appl Environ Microbiol 74:6444-6. 2008..Many sequences were shared between evaporites, which suggests that similar environments select for specific microbial lineages from a global metacommunity... - Microbial diversity of cryptoendolithic communities from the McMurdo Dry Valleys, AntarcticaJosé R de la Torre
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720 3102, USA
Appl Environ Microbiol 69:3858-67. 2003.... - Microbial community biofabrics in a geothermal mine aditJohn R Spear
Division of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401, USA
Appl Environ Microbiol 73:6172-80. 2007..These two novel types of biofabrics are examples of the complex roles that microbes can play in mineralization, weathering, and deposition processes in karst environments... - Molecular analysis of shower curtain biofilm microbesScott T Kelley
Department of Biology, San Diego State University, California 92182, USA
Appl Environ Microbiol 70:4187-92. 2004..Frequent cleaning or disposal of shower curtains is indicated, particularly in households with immune-compromised individuals... - Polyphosphate kinase from activated sludge performing enhanced biological phosphorus removalKatherine D McMahon
Department of Civil Engineering, University of California at Berkeley, Berkeley, California 94720-1460, USA
Appl Environ Microbiol 68:4971-8. 2002..The purified PPK has a specific activity comparable to that of other PPKs, has a requirement for Mg(2+), and does not appear to operate in reverse. PPK activity was found mainly in the particulate fraction of lysed sludge microorganisms... - Novel kingdom-level eukaryotic diversity in anoxic environmentsScott C Dawson
Department of Molecular and Cell Biology, 345 LSA Building, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 99:8324-9. 2002..Phylogenetic analyses with the new sequences enhance our understanding of the diversity and pattern of eukaryotic evolution... - An open letter to Elias ZerhouniSidney Altman
Science 307:1409-10. 2005 - Molecular identification of potential pathogens in water and air of a hospital therapy poolLargus T Angenent
Department of Chemical Engineering and Environmental Engineering Science Program, Washington University, St Louis, MO 63130, USA
Proc Natl Acad Sci U S A 102:4860-5. 2005..The results also show that culture protocols currently used by public health facilities and agencies are seriously inadequate for the detection and enumeration of potential pathogens... - The genetic core of the universal ancestorJ Kirk Harris
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
Genome Res 13:407-12. 2003..The analysis also identified several genes of unknown function with phylogenies that track with the ribosomal RNA genes. The products of these genes are likely to play fundamental roles in cellular processes... - Metagenomic approaches for defining the pathogenesis of inflammatory bowel diseasesDaniel A Peterson
Center for Genome Sciences, Washington University School of Medicine, St Louis, MO 63108, USA
Cell Host Microbe 3:417-27. 2008.... - Community and cultivation analysis of arsenite oxidizing biofilms at Hot CreekTina M Salmassi
Environmental Science and Engineering, California Institute of Technology, Pasadena, 91125, USA
Environ Microbiol 8:50-9. 2006..This estimate represents the first normalization of arsenite oxidation rates to MPN cell densities for a microbial community in a field incubation experiment...
Research Grants
- Microbiological Characterization and Mitigation of Bioaerosols in CAFOsNorman Pace; Fiscal Year: 2009..4. Determine the impact on total and viable microbial loads in CAFOs following mitigation with U.V. irradiation and electrical discharge methods of bio-aerosol disinfection. ..
- Microbiological Characterization and Mitigation of Bioaerosols in CAFOsNorman Pace; Fiscal Year: 2007..4. Determine the impact on total and viable microbial loads in CAFOs following mitigation with U.V. irradiation and electrical discharge methods of bio-aerosol disinfection. ..
- FUNCTION AND METABOLISM OF RNANorman Pace; Fiscal Year: 2007..The hyperthermophilic character of this archaeal RNaseP may render it more amenable than the eukaryotic enzymes to procedures such as reconstitution from isolated components and crystallization for structure analysis. ..
- Molecular Analysis of Microbes in Chronic Bowel DiseaseNorman Pace; Fiscal Year: 2004..abstract_text> ..
- FUNCTION AND METABOLISM OF RNANorman R Pace; Fiscal Year: 2010..RNase P is universally distributed, required for all cellular growth and development, and potentially is a target for organism-specific antibiotic therapies. ..