Norman Pace

Summary

Affiliation: University of Colorado
Country: USA

Publications

  1. ncbi request reprint RNase P: interface of the RNA and protein worlds
    Donald Evans
    Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Campus Box 347, Boulder, CO 80309 0347, USA
    Trends Biochem Sci 31:333-41. 2006
  2. ncbi request reprint Gastrointestinal microbiology enters the metagenomics era
    Daniel N Frank
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Curr Opin Gastroenterol 24:4-10. 2008
  3. ncbi request reprint Q and A
    Norman Pace
    Department of Molecular, Cellular and Development Biology, University of Colarado 80309 0347, USA
    Curr Biol 16:R5-R6. 2006
  4. pmc Mapping the tree of life: progress and prospects
    Norman R Pace
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Microbiol Mol Biol Rev 73:565-76. 2009
  5. pmc Phylogenetic composition of Rocky Mountain endolithic microbial ecosystems
    Jeffrey J Walker
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Appl Environ Microbiol 73:3497-504. 2007
  6. ncbi request reprint Endolithic microbial ecosystems
    Jeffrey J Walker
    University of Colorado at Boulder, Department of Molecular, Cellular and Developmental Biology, Boulder, Colorado 80309 0347, USA
    Annu Rev Microbiol 61:331-47. 2007
  7. doi request reprint Disease phenotype and genotype are associated with shifts in intestinal-associated microbiota in inflammatory bowel diseases
    Daniel N Frank
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80045, USA
    Inflamm Bowel Dis 17:179-84. 2011
  8. pmc Hydrogen and bioenergetics in the Yellowstone geothermal ecosystem
    John R Spear
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 102:2555-60. 2005
  9. pmc Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia
    Dominic Papineau
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Appl Environ Microbiol 71:4822-32. 2005
  10. pmc Unexpected diversity and complexity of the Guerrero Negro hypersaline microbial mat
    Ruth E Ley
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Appl Environ Microbiol 72:3685-95. 2006

Collaborators

Detail Information

Publications43

  1. ncbi request reprint RNase P: interface of the RNA and protein worlds
    Donald Evans
    Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Campus Box 347, Boulder, CO 80309 0347, USA
    Trends Biochem Sci 31:333-41. 2006
    ..In vitro reconstitution of activity is providing insight into the role of proteins in the RNase P holoenzyme. Together, these findings are beginning to impart an understanding of the coevolution of the RNA and protein worlds...
  2. ncbi request reprint Gastrointestinal microbiology enters the metagenomics era
    Daniel N Frank
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Curr Opin Gastroenterol 24:4-10. 2008
    ..This review summarizes the molecular methods of culture-independent microbiology ('metagenomics') and their recent application to studies of the human gastrointestinal tract in both health and disease...
  3. ncbi request reprint Q and A
    Norman Pace
    Department of Molecular, Cellular and Development Biology, University of Colarado 80309 0347, USA
    Curr Biol 16:R5-R6. 2006
  4. pmc Mapping the tree of life: progress and prospects
    Norman R Pace
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Microbiol Mol Biol Rev 73:565-76. 2009
    ..Obstacles to accurate inference of deep phylogenetic relationships are both systematic, in molecular phylogenetic calculations, and practical, due to a paucity of sequence representation for many groups of organisms...
  5. pmc Phylogenetic composition of Rocky Mountain endolithic microbial ecosystems
    Jeffrey J Walker
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Appl Environ Microbiol 73:3497-504. 2007
    ..Collectively, results of this study support the idea that patterns of microbial diversity found in endolithic communities are governed by principles similar to those observed in macroecological systems...
  6. ncbi request reprint Endolithic microbial ecosystems
    Jeffrey J Walker
    University of Colorado at Boulder, Department of Molecular, Cellular and Developmental Biology, Boulder, Colorado 80309 0347, USA
    Annu Rev Microbiol 61:331-47. 2007
    ..Recent results also explore geobiological processes that shape the current biosphere and potentially provide clues to life's history on Earth and where to seek life elsewhere in the Solar System...
  7. doi request reprint Disease phenotype and genotype are associated with shifts in intestinal-associated microbiota in inflammatory bowel diseases
    Daniel N Frank
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80045, USA
    Inflamm Bowel Dis 17:179-84. 2011
    ..We hypothesize that NOD2 and ATG16L1 risk alleles may be associated with these alterations...
  8. pmc Hydrogen and bioenergetics in the Yellowstone geothermal ecosystem
    John R Spear
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 102:2555-60. 2005
    ....
  9. pmc Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia
    Dominic Papineau
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Appl Environ Microbiol 71:4822-32. 2005
    ..These results provide a framework for understanding the kinds of organisms that build contemporary stromatolites, their ecology, and their relevance to stromatolites preserved in the geological record...
  10. pmc Unexpected diversity and complexity of the Guerrero Negro hypersaline microbial mat
    Ruth E Ley
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Appl Environ Microbiol 72:3685-95. 2006
    ..The sequences determined in this study have been submitted to the GenBank database and assigned accession numbers DQ 329539 to DQ 331020, and DQ 397339 to DQ 397511...
  11. ncbi request reprint Phylogenetic diversity and ecology of environmental Archaea
    Charles E Robertson
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Curr Opin Microbiol 8:638-42. 2005
    ..Although it is apparent that Archaea can be found in all environments, the chemistry of their ecological context is mostly unknown...
  12. pmc Diversity and stratification of archaea in a hypersaline microbial mat
    Charles E Robertson
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Appl Environ Microbiol 75:1801-10. 2009
    ..Some clades did not segregate by depth in the mat, indicating broad metabolic repertoires, undersampling, or both...
  13. ncbi request reprint Time for a change
    Norman R Pace
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309 0347, USA
    Nature 441:289. 2006
  14. ncbi request reprint Complexity in natural microbial ecosystems: the Guerrero Negro experience
    John R Spear
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, 347 UCB, Boulder, Colorado 80309, USA
    Biol Bull 204:168-73. 2003
    ..To date, analyses of these two environments indicate the ubiquitous dominance of uncultured organisms of phylogenetic kinds not generally thought to be associated with hypersaline environments...
  15. ncbi request reprint Bacterial RNase P: a new view of an ancient enzyme
    Alexei V Kazantsev
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Nat Rev Microbiol 4:729-40. 2006
    ..The goal of the present review is to summarize what is known about the bacterial RNase P, and to bring together the recent structural results with extensive earlier biochemical and phylogenetic findings...
  16. ncbi request reprint Geobiology of a microbial endolithic community in the Yellowstone geothermal environment
    Jeffrey J Walker
    Department of Molecular, Cellular and Developmental Biology and the Center for Astrobiology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Nature 434:1011-4. 2005
    ..Remnants of these communities could serve as biosignatures and provide important clues about ancient life associated with geothermal environments on the Earth or elsewhere in the Solar System...
  17. pmc New perspective on uncultured bacterial phylogenetic division OP11
    J Kirk Harris
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Appl Environ Microbiol 70:845-9. 2004
    ....
  18. pmc Culture-independent microbiological analysis of foley urinary catheter biofilms
    Daniel N Frank
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO, USA
    PLoS ONE 4:e7811. 2009
    ..Prevention of catheter-associated urinary tract infection (CAUTI), a leading cause of nosocomial disease, is complicated by the propensity of bacteria to form biofilms on indwelling medical devices [1,2,3,4,5]...
  19. pmc Opportunistic pathogens enriched in showerhead biofilms
    Leah M Feazel
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Proc Natl Acad Sci U S A 106:16393-9. 2009
    ..The health risk associated with showerhead microbiota needs investigation in persons with compromised immune or pulmonary systems...
  20. pmc Culture-independent molecular analysis of microbial constituents of the healthy human outer ear
    Daniel N Frank
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    J Clin Microbiol 41:295-303. 2003
    ..Alternatively, culture analyses of diseases of the middle ear may have been confounded by these contaminating commensal organisms...
  21. pmc Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases
    Daniel N Frank
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Proc Natl Acad Sci U S A 104:13780-5. 2007
    ..Patient stratification by GI microbiota provides further evidence that CD represents a spectrum of disease states and suggests that treatment of some forms of IBD may be facilitated by redress of the detected microbiological imbalances...
  22. pmc High-resolution structure of RNase P protein from Thermotoga maritima
    Alexei V Kazantsev
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Proc Natl Acad Sci U S A 100:7497-502. 2003
    ..The protein is found to dimerize through an extensive, well-ordered interface. This dimerization may reflect a mechanism of thermal stability of the protein before assembly with the RNA moiety of the holoenzyme...
  23. pmc Eucaryotic diversity in a hypersaline microbial mat
    Leah M Feazel
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 90309 0347, USA
    Appl Environ Microbiol 74:329-32. 2008
    ..Six eucaryotic kingdoms were represented, as well as a novel cluster of sequences. Nematode sequences dominated the clone libraries...
  24. pmc Molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosis
    J Kirk Harris
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 104:20529-33. 2007
    ..These results show that molecular techniques provide a broader perspective on airway bacteria than do routine clinical cultures and thus can identify targets for further clinical evaluation...
  25. pmc The human nasal microbiota and Staphylococcus aureus carriage
    Daniel N Frank
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, Colorado, United States of America
    PLoS ONE 5:e10598. 2010
    ..aureus colonization we determined the composition and dynamics of the nasal microbiota and correlated specific microorganisms with S. aureus colonization...
  26. pmc Use of specific rRNA oligonucleotide probes for microscopic detection of Mycobacterium avium complex organisms in tissue
    Allison L St Amand
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    J Clin Microbiol 43:1505-14. 2005
    ..intracellulare, the two M. avium subspecies associated with human disease, or all members of MAC. The results call into question the validity of ISH results derived by the use of other gene loci, such as IS900...
  27. pmc Culture-independent analysis of indomethacin-induced alterations in the rat gastrointestinal microbiota
    Andrew B Dalby
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Appl Environ Microbiol 72:6707-15. 2006
    ..These data suggest that treatment of NSAID-induced ulceration may be facilitated by addressing the microbiological imbalances...
  28. doi request reprint Microbial diversity in chronic open wounds
    Daniel N Frank
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, USA
    Wound Repair Regen 17:163-72. 2009
    ....
  29. pmc Structural implications of novel diversity in eucaryal RNase P RNA
    Steven M Marquez
    Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Room A3B40, Boulder, CO 80309 0347, USA
    RNA 11:739-51. 2005
    ..The eucaryal RNA has been remodeled through evolution as a simplified version of the structure found in bacterial and archaeal RNase P RNAs...
  30. pmc Use of specific rRNA oligonucleotide probes for microscopic detection of Mycobacterium tuberculosis in culture and tissue specimens
    Allison L St Amand
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    J Clin Microbiol 43:5369-71. 2005
    ..We report a rapid and reliable in situ hybridization technique using rRNA oligonucleotide probes for the identification of M. tuberculosis in tissues and cultures...
  31. ncbi request reprint Structural perspective on the activation of RNAse P RNA by protein
    Amy H Buck
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA
    Nat Struct Mol Biol 12:958-64. 2005
    ..The data are consistent with a role of the protein in substrate recognition and support a new model of the holoenzyme that is based on a recently solved crystal structure of RNase P RNA...
  32. pmc Microbial composition of near-boiling silica-depositing thermal springs throughout Yellowstone National Park
    Carrine E Blank
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA
    Appl Environ Microbiol 68:5123-35. 2002
    ..Therefore, species- or subspecies-level divergences are present but not detectable in highly conserved SSU sequences...
  33. pmc Comparative molecular analysis of endoevaporitic microbial communities
    Jason W Sahl
    Department of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401, USA
    Appl Environ Microbiol 74:6444-6. 2008
    ..Many sequences were shared between evaporites, which suggests that similar environments select for specific microbial lineages from a global metacommunity...
  34. pmc Microbial diversity of cryptoendolithic communities from the McMurdo Dry Valleys, Antarctica
    José R de la Torre
    Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720 3102, USA
    Appl Environ Microbiol 69:3858-67. 2003
    ....
  35. pmc Microbial community biofabrics in a geothermal mine adit
    John R Spear
    Division of Environmental Science and Engineering, Colorado School of Mines, Golden, Colorado 80401, USA
    Appl Environ Microbiol 73:6172-80. 2007
    ..These two novel types of biofabrics are examples of the complex roles that microbes can play in mineralization, weathering, and deposition processes in karst environments...
  36. pmc Molecular analysis of shower curtain biofilm microbes
    Scott T Kelley
    Department of Biology, San Diego State University, California 92182, USA
    Appl Environ Microbiol 70:4187-92. 2004
    ..Frequent cleaning or disposal of shower curtains is indicated, particularly in households with immune-compromised individuals...
  37. pmc Polyphosphate kinase from activated sludge performing enhanced biological phosphorus removal
    Katherine D McMahon
    Department of Civil Engineering, University of California at Berkeley, Berkeley, California 94720 1460, USA
    Appl Environ Microbiol 68:4971-8. 2002
    ..The purified PPK has a specific activity comparable to that of other PPKs, has a requirement for Mg(2+), and does not appear to operate in reverse. PPK activity was found mainly in the particulate fraction of lysed sludge microorganisms...
  38. pmc Novel kingdom-level eukaryotic diversity in anoxic environments
    Scott C Dawson
    Department of Molecular and Cell Biology, 345 LSA Building, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 99:8324-9. 2002
    ..Phylogenetic analyses with the new sequences enhance our understanding of the diversity and pattern of eukaryotic evolution...
  39. ncbi request reprint An open letter to Elias Zerhouni
    Sidney Altman
    Science 307:1409-10. 2005
  40. pmc Molecular identification of potential pathogens in water and air of a hospital therapy pool
    Largus T Angenent
    Department of Chemical Engineering and Environmental Engineering Science Program, Washington University, St Louis, MO 63130, USA
    Proc Natl Acad Sci U S A 102:4860-5. 2005
    ..The results also show that culture protocols currently used by public health facilities and agencies are seriously inadequate for the detection and enumeration of potential pathogens...
  41. pmc The genetic core of the universal ancestor
    J Kirk Harris
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Genome Res 13:407-12. 2003
    ..The analysis also identified several genes of unknown function with phylogenies that track with the ribosomal RNA genes. The products of these genes are likely to play fundamental roles in cellular processes...
  42. pmc Metagenomic approaches for defining the pathogenesis of inflammatory bowel diseases
    Daniel A Peterson
    Center for Genome Sciences, Washington University School of Medicine, St Louis, MO 63108, USA
    Cell Host Microbe 3:417-27. 2008
    ....
  43. ncbi request reprint Community and cultivation analysis of arsenite oxidizing biofilms at Hot Creek
    Tina M Salmassi
    Environmental Science and Engineering, California Institute of Technology, Pasadena, 91125, USA
    Environ Microbiol 8:50-9. 2006
    ..This estimate represents the first normalization of arsenite oxidation rates to MPN cell densities for a microbial community in a field incubation experiment...

Research Grants13

  1. Microbiological Characterization and Mitigation of Bioaerosols in CAFOs
    Norman Pace; Fiscal Year: 2009
    ..4. Determine the impact on total and viable microbial loads in CAFOs following mitigation with U.V. irradiation and electrical discharge methods of bio-aerosol disinfection. ..
  2. Microbiological Characterization and Mitigation of Bioaerosols in CAFOs
    Norman Pace; Fiscal Year: 2007
    ..4. Determine the impact on total and viable microbial loads in CAFOs following mitigation with U.V. irradiation and electrical discharge methods of bio-aerosol disinfection. ..
  3. FUNCTION AND METABOLISM OF RNA
    Norman Pace; Fiscal Year: 2007
    ..The hyperthermophilic character of this archaeal RNaseP may render it more amenable than the eukaryotic enzymes to procedures such as reconstitution from isolated components and crystallization for structure analysis. ..
  4. Molecular Analysis of Microbes in Chronic Bowel Disease
    Norman Pace; Fiscal Year: 2004
    ..abstract_text> ..
  5. FUNCTION AND METABOLISM OF RNA
    Norman R Pace; Fiscal Year: 2010
    ..RNase P is universally distributed, required for all cellular growth and development, and potentially is a target for organism-specific antibiotic therapies. ..