Andre J Ouellette

Summary

Affiliation: University of Southern California
Country: USA

Publications

  1. ncbi request reprint Paneth cell α-defensins in enteric innate immunity
    André Joseph Ouellette
    Department of Pathology and Laboratory Medicine, Keck School of Medicine of the University of Southern California, USC Norris Cancer Center, Los Angeles, CA 90089 9601, USA
    Cell Mol Life Sci 68:2215-29. 2011
  2. doi request reprint Paneth cells and innate mucosal immunity
    Andre J Ouellette
    Department of Pathology and Laboratory Medicine, Keck School of Medicine of the University of Southern California, Los Angeles, California 90089 9601, USA
    Curr Opin Gastroenterol 26:547-53. 2010
  3. pmc Arginine in α-defensins: differential effects on bactericidal activity correspond to geometry of membrane curvature generation and peptide-lipid phase behavior
    Nathan W Schmidt
    Department of Bioengineering, UCLA, Los Angeles, California 90095 1600, USA
    J Biol Chem 287:21866-72. 2012
  4. pmc High fidelity processing and activation of the human α-defensin HNP1 precursor by neutrophil elastase and proteinase 3
    Prasad Tongaonkar
    Department of Pathology and Laboratory Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 7:e32469. 2012
  5. ncbi request reprint Hydrophobic determinants of α-defensin bactericidal activity
    Kenneth P Tai
    Department of Pathology and Laboratory Medicine, University of Southern California Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California
    Infect Immun 82:2195-202. 2014
  6. pmc Rhesus macaque theta defensins suppress inflammatory cytokines and enhance survival in mouse models of bacteremic sepsis
    Justin B Schaal
    Department of Pathology and Laboratory Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 7:e51337. 2012
  7. pmc Strain-specific polymorphisms in Paneth cell α-defensins of C57BL/6 mice and evidence of vestigial myeloid α-defensin pseudogenes
    Michael T Shanahan
    Department of Pathology and Laboratory Medicine, Keck School of Medicine of the University of Southern California, USC Norris Cancer Center, NRT 7514, 1450 Biggy Street, Los Angeles, CA 90089 9601, USA
    Infect Immun 79:459-73. 2011
  8. pmc Inhibition of bactericidal activity is maintained in a mouse alpha-defensin precursor with proregion truncations
    Sharel M Figueredo
    Department of Pathology and Laboratory Medicine, School of Medicine, College of Health Sciences, University of California, Irvine, CA 92697 4800, USA
    Peptides 31:9-15. 2010
  9. pmc Electropositive charge in alpha-defensin bactericidal activity: functional effects of Lys-for-Arg substitutions vary with the peptide primary structure
    R Alan Llenado
    Department of Pathology and Laboratory Medicine, School of Medicine, University of California, Irvine, CA 92697 4800, USA
    Infect Immun 77:5035-43. 2009
  10. pmc Rhesus macaque θ-defensin isoforms: expression, antimicrobial activities, and demonstration of a prominent role in neutrophil granule microbicidal activities
    Prasad Tongaonkar
    Department of Pathology and Laboratory Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA
    J Leukoc Biol 89:283-90. 2011

Research Grants

  1. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 2009
  2. STUDIES OF ALPHA-DEFENSINS IN PRIMATE INNATE IMMUNITY
    Andre J Ouellette; Fiscal Year: 2010
  3. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre J Ouellette; Fiscal Year: 2010
  4. STUDIES OF ALPHA-DEFENSINS IN PRIMATE INNATE IMMUNITY
    Andre Ouellette; Fiscal Year: 2009
  5. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 2007
  6. STUDIES OF ALPHA-DEFENSINS IN PRIMATE INNATE IMMUNITY
    Andre Ouellette; Fiscal Year: 2006
  7. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 2006
  8. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 1999
  9. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 2009

Collaborators

  • Michael E Selsted
  • Prasad Tongaonkar
  • Hiroki Tanabe
  • Julio A Camarero
  • Raz Jelinek
  • Kenneth P Tai
  • Jennifer R Mastroianni
  • Sharel M Figueredo
  • Colby S Weeks
  • Nathan W Schmidt
  • Abhijit Mishra
  • Karishma Kamdar
  • Justin B Schaal
  • Gerard C L Wong
  • Michael T Shanahan
  • Angie E Garcia
  • Sharel Figueredo
  • R Alan Llenado
  • Steven K Young
  • Melanie J Cocco
  • Valerie V Le
  • Katie Trinh
  • Dat Tran
  • Nita H Salzman
  • Kathleen M Brasky
  • Kevin D Roberts
  • Jessica K Costales
  • Jennifer Zaksheske
  • George Osapay
  • Patti Tran
  • Kun Zhao
  • Ghee Hwee Lai
  • Shadakshara S Puttamadappa
  • Alexander Shekhtman
  • Atsuo Maemoto
  • Xiaoqing Qu
  • Tanya Sheynis
  • Jason E Cummings
  • T Kyle Vanderlick
  • Steve P Crampton

Detail Information

Publications19

  1. ncbi request reprint Paneth cell α-defensins in enteric innate immunity
    André Joseph Ouellette
    Department of Pathology and Laboratory Medicine, Keck School of Medicine of the University of Southern California, USC Norris Cancer Center, Los Angeles, CA 90089 9601, USA
    Cell Mol Life Sci 68:2215-29. 2011
    ..Disruption of Paneth cell homeostasis, with subsequent induction of endoplasmic reticulum stress, autophagy, or apoptosis, contributes to inflammation in diverse genetic and experimental mouse models...
  2. doi request reprint Paneth cells and innate mucosal immunity
    Andre J Ouellette
    Department of Pathology and Laboratory Medicine, Keck School of Medicine of the University of Southern California, Los Angeles, California 90089 9601, USA
    Curr Opin Gastroenterol 26:547-53. 2010
    ..Recent evidence shows that disruption of Paneth cell homeostasis by induction of endoplasmic reticulum stress or autophagy, with consequent apoptosis, contributes to inflammation and morbidity in a variety of experimental mouse models...
  3. pmc Arginine in α-defensins: differential effects on bactericidal activity correspond to geometry of membrane curvature generation and peptide-lipid phase behavior
    Nathan W Schmidt
    Department of Bioengineering, UCLA, Los Angeles, California 90095 1600, USA
    J Biol Chem 287:21866-72. 2012
    ....
  4. pmc High fidelity processing and activation of the human α-defensin HNP1 precursor by neutrophil elastase and proteinase 3
    Prasad Tongaonkar
    Department of Pathology and Laboratory Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 7:e32469. 2012
    ..Thus, NE and PR3 possess proHNP1 convertase activity that requires the presence of the native HNP1 disulfide motif for high fidelity activation of the precursor in vitro...
  5. ncbi request reprint Hydrophobic determinants of α-defensin bactericidal activity
    Kenneth P Tai
    Department of Pathology and Laboratory Medicine, University of Southern California Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California
    Infect Immun 82:2195-202. 2014
    ....
  6. pmc Rhesus macaque theta defensins suppress inflammatory cytokines and enhance survival in mouse models of bacteremic sepsis
    Justin B Schaal
    Department of Pathology and Laboratory Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 7:e51337. 2012
    ..Collectively, these results indicate that the anti-inflammatory properties of θ-defensins in vitro and in vivo are mediated by the suppression of numerous proinflammatory cytokines and blockade of TNF release may be a primary effect...
  7. pmc Strain-specific polymorphisms in Paneth cell α-defensins of C57BL/6 mice and evidence of vestigial myeloid α-defensin pseudogenes
    Michael T Shanahan
    Department of Pathology and Laboratory Medicine, Keck School of Medicine of the University of Southern California, USC Norris Cancer Center, NRT 7514, 1450 Biggy Street, Los Angeles, CA 90089 9601, USA
    Infect Immun 79:459-73. 2011
    ..Given the role of α-defensins in shaping the composition of the enteric microflora, such polymorphisms may influence outcomes in mouse models of disease or infection...
  8. pmc Inhibition of bactericidal activity is maintained in a mouse alpha-defensin precursor with proregion truncations
    Sharel M Figueredo
    Department of Pathology and Laboratory Medicine, School of Medicine, College of Health Sciences, University of California, Irvine, CA 92697 4800, USA
    Peptides 31:9-15. 2010
    ..Therefore, the conserved distance that separates inhibitory acidic proregion residues from the Crp4 peptide is not critical to maintaining proCrp4((20-92)) in an inactive state...
  9. pmc Electropositive charge in alpha-defensin bactericidal activity: functional effects of Lys-for-Arg substitutions vary with the peptide primary structure
    R Alan Llenado
    Department of Pathology and Laboratory Medicine, School of Medicine, University of California, Irvine, CA 92697 4800, USA
    Infect Immun 77:5035-43. 2009
    ....
  10. pmc Rhesus macaque θ-defensin isoforms: expression, antimicrobial activities, and demonstration of a prominent role in neutrophil granule microbicidal activities
    Prasad Tongaonkar
    Department of Pathology and Laboratory Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA
    J Leukoc Biol 89:283-90. 2011
    ..Supplementation of human granule extracts with RTD-1 markedly increased the microbicidal activity of these preparations, further demonstrating a prominent microbicidal role for θ-defensins...
  11. doi request reprint Expression and purification of recombinant alpha-defensins and alpha-defensin precursors in Escherichia coli
    Sharel Figueredo
    Department of Pathology and Laboratory Medicine, School of Medicine, College of Health Sciences, University of California, Irvine, CA, USA
    Methods Mol Biol 618:47-60. 2010
    ..alpha-Defensins prepared in this manner are biochemically equivalent to the natural molecules...
  12. pmc In vitro activation of the rhesus macaque myeloid alpha-defensin precursor proRMAD-4 by neutrophil serine proteinases
    Karishma Kamdar
    Department of Pathology and Laboratory Medicine, School of Medicine, College of Health Sciences, University of California, Irvine, California 92697 4800, USA
    J Biol Chem 283:32361-8. 2008
    ..These in vitro analyses support the conclusion that rhesus macaque myeloid pro-alpha-defensins are converted to active forms by serine proteinases that co-localize in azurophil granules...
  13. pmc Alternative luminal activation mechanisms for paneth cell α-defensins
    Jennifer R Mastroianni
    Department of Pathology and Laboratory Medicine and the USC Norris Cancer Center Keck School of Medicine of The University of Southern California, Los Angeles, California 90089 9601, USA
    J Biol Chem 287:11205-12. 2012
    ..MMP7(-/-) mice only lack processed α-defensins in the small intestine, and the model is not appropriate for studying effects of α-defensin deficiency in cecal or colonic infection or disease...
  14. pmc Biosynthesis and antimicrobial evaluation of backbone-cyclized α-defensins
    Angie E Garcia
    Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, Calofornia 90033, USA
    Biochemistry 50:10508-19. 2011
    ..Altogether, these results indicate the potential for backbone-cyclized defensins in the development of novel peptide-based antimicrobial compounds...
  15. pmc Anionic amino acids near the pro-alpha-defensin N terminus mediate inhibition of bactericidal activity in mouse pro-cryptdin-4
    Sharel M Figueredo
    Department of Pathology and Laboratory Medicine, University of California, Irvine, California 92697 4800, USA
    J Biol Chem 284:6826-31. 2009
    ..These findings support the conclusion that the most distal N-terminal anionic residues of pro-Crp4-(20-92) are primarily responsible for blocking Crp4-mediated membrane disruption in the precursor...
  16. pmc Alpha-defensins in enteric innate immunity: functional Paneth cell alpha-defensins in mouse colonic lumen
    Jennifer R Mastroianni
    Department of Pathology and Laboratory Medicine, University of California, Irvine, California 92697 4800, USA
    J Biol Chem 284:27848-56. 2009
    ....
  17. doi request reprint Small-angle X-ray scattering studies of peptide-lipid interactions using the mouse paneth cell α-defensin cryptdin-4
    Abhijit Mishra
    Department of Bioengineering, University of California, Los Angeles, California, USA
    Methods Enzymol 492:127-49. 2011
    ....
  18. ncbi request reprint Matrix metalloproteinase-7 activation of mouse paneth cell pro-alpha-defensins: SER43 down arrow ILE44 proteolysis enables membrane-disruptive activity
    Colby S Weeks
    Department of Pathology and Laboratory Medicine, Molecular Biology and Biochemistry, and Microbiology and Molecular Genetics, and Center for Immunology, University of California, Irvine, California 92697 4800, USA
    J Biol Chem 281:28932-42. 2006
    ....
  19. ncbi request reprint Mammalian defensins in the antimicrobial immune response
    Michael E Selsted
    Department of Pathology and Laboratory Medicine, and Center for Immunology, School of Medicine, University of California, Irvine, California 92697, USA
    Nat Immunol 6:551-7. 2005
    ..This review focuses on the biological functions of three structural subgroups of mammalian defensins and the evidence for their involvement as effectors of antimicrobial innate immunity...

Research Grants17

  1. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 2009
    ..Knowledge of such mechanisms is essential to augment innate defenses and to insure compatibility of new therapeutics with endogenous effectors of host defense. ..
  2. STUDIES OF ALPHA-DEFENSINS IN PRIMATE INNATE IMMUNITY
    Andre J Ouellette; Fiscal Year: 2010
    ..Potential lineage-specific gene regulatory regions will be assayed by functional analyses of RED and RMAD promoter constructs and chimeric minigenes in cultured monocytes. ..
  3. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre J Ouellette; Fiscal Year: 2010
    ..Knowledge of such mechanisms is essential to augment innate defenses and to insure compatibility of new therapeutics with endogenous effectors of host defense. ..
  4. STUDIES OF ALPHA-DEFENSINS IN PRIMATE INNATE IMMUNITY
    Andre Ouellette; Fiscal Year: 2009
    ..Potential lineage-specific gene regulatory regions will be assayed by functional analyses of RED and RMAD promoter constructs and chimeric minigenes in cultured monocytes. ..
  5. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 2007
    ..Knowledge of such mechanisms is essential to augment innate defenses and to insure compatibility of new therapeutics with endogenous effectors of host defense. ..
  6. STUDIES OF ALPHA-DEFENSINS IN PRIMATE INNATE IMMUNITY
    Andre Ouellette; Fiscal Year: 2006
    ..Potential lineage-specific gene regulatory regions will be assayed by functional analyses of RED and RMAD promoter constructs and chimeric minigenes in cultured monocytes. ..
  7. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 2006
    ..Knowledge of such mechanisms is essential to augment innate defenses and to insure compatibility of new therapeutics with endogenous effectors of host defense. ..
  8. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 1999
    ....
  9. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre Ouellette; Fiscal Year: 2009
    ..Knowledge of such mechanisms is essential to augment innate defenses and to insure compatibility of new therapeutics with endogenous effectors of host defense. ..