T S Otis

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc GABAA receptors increase excitability and conduction velocity of cerebellar parallel fiber axons
    Shlomo S Dellal
    Dept of Neurobiology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA
    J Neurophysiol 107:2958-70. 2012
  2. ncbi request reprint Anion currents and predicted glutamate flux through a neuronal glutamate transporter
    T S Otis
    Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Neurosci 18:7099-110. 1998
  3. ncbi request reprint Interactions between glutamate transporters and metabotropic glutamate receptors at excitatory synapses in the cerebellar cortex
    Thomas S Otis
    Department of Neurobiology and Brain Research Institute, School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Neurochem Int 45:537-44. 2004
  4. ncbi request reprint Isolation of current components and partial reaction cycles in the glial glutamate transporter EAAT2
    T S Otis
    Department of Neurobiology, University of California, Los Angeles Medical Center, Los Angeles, California 90095 1763, USA
    J Neurosci 20:2749-57. 2000
  5. ncbi request reprint Neuronal glutamate transporters control activation of postsynaptic metabotropic glutamate receptors and influence cerebellar long-term depression
    G Brasnjo
    Department of Neurobiology and Brain Research Institute, School of Medicine, University of California, Los Angeles 90095, USA
    Neuron 31:607-16. 2001
  6. ncbi request reprint Climbing fiber activation of metabotropic glutamate receptors on cerebellar purkinje neurons
    Jeffrey A Dzubay
    Department of Neurobiology and the Brain Research Institute, School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Neuron 36:1159-67. 2002
  7. ncbi request reprint Expression of GFP-tagged neuronal glutamate transporters in cerebellar Purkinje neurons
    Pratap Meera
    Department of Neurobiology and Brain Research Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Neuropharmacology 49:883-9. 2005
  8. ncbi request reprint Whither withered Golgi? A retrospective evaluation of reticularist and synaptic constructs
    Lawrence Kruger
    Department of Neurobiology, UCLA David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Brain Res Bull 72:201-7. 2007
  9. ncbi request reprint An ultra small array of electrodes for stimulating multiple inputs into a single neuron
    Spencer L Smith
    Neuroscience and Neuroengineering Programs, 1320 Gonda Center, 695 Young Drive South, University of California, Los Angeles, CA 90095, USA
    J Neurosci Methods 133:109-14. 2004
  10. pmc Isolation of glutamate transport-coupled charge flux and estimation of glutamate uptake at the climbing fiber-Purkinje cell synapse
    Gabor Brasnjo
    Department of Neurobiology, School of Medicine, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 101:6273-8. 2004

Collaborators

Detail Information

Publications20

  1. pmc GABAA receptors increase excitability and conduction velocity of cerebellar parallel fiber axons
    Shlomo S Dellal
    Dept of Neurobiology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA
    J Neurophysiol 107:2958-70. 2012
    ....
  2. ncbi request reprint Anion currents and predicted glutamate flux through a neuronal glutamate transporter
    T S Otis
    Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Neurosci 18:7099-110. 1998
    ..Finally, a fixed proportion of anion and glutamate flux is expected over a wide range of circumstances, providing theoretical support for using net charge flux to estimate the amount and time course of glutamate transport...
  3. ncbi request reprint Interactions between glutamate transporters and metabotropic glutamate receptors at excitatory synapses in the cerebellar cortex
    Thomas S Otis
    Department of Neurobiology and Brain Research Institute, School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Neurochem Int 45:537-44. 2004
    ..g. selective serotonin reuptake inhibitors) and drugs of abuse (amphetamine, cocaine), little is known about how they operate at a molecular level or contribute to synaptic transmission...
  4. ncbi request reprint Isolation of current components and partial reaction cycles in the glial glutamate transporter EAAT2
    T S Otis
    Department of Neurobiology, University of California, Los Angeles Medical Center, Los Angeles, California 90095 1763, USA
    J Neurosci 20:2749-57. 2000
    ..The kinetic properties of EAAT2, the dominant glutamate transporter in brain astrocytes, distinguish it as an efficient sink for synaptically released glutamate...
  5. ncbi request reprint Neuronal glutamate transporters control activation of postsynaptic metabotropic glutamate receptors and influence cerebellar long-term depression
    G Brasnjo
    Department of Neurobiology and Brain Research Institute, School of Medicine, University of California, Los Angeles 90095, USA
    Neuron 31:607-16. 2001
    ..Postsynaptic glutamate uptake may serve as a general mechanism for regulating mGluR-initiated synaptic depression...
  6. ncbi request reprint Climbing fiber activation of metabotropic glutamate receptors on cerebellar purkinje neurons
    Jeffrey A Dzubay
    Department of Neurobiology and the Brain Research Institute, School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Neuron 36:1159-67. 2002
    ..This additional target for glutamate broadens the signaling capabilities of CF synapses and raises the possibility that changes in CF strength are homosynaptically triggered...
  7. ncbi request reprint Expression of GFP-tagged neuronal glutamate transporters in cerebellar Purkinje neurons
    Pratap Meera
    Department of Neurobiology and Brain Research Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Neuropharmacology 49:883-9. 2005
    ..These findings indicate that functional EAAT fusion proteins can be synthesized and appropriately trafficked to postsynaptic compartments. Furthermore, they validate a powerful system for looking at EAAT function in situ...
  8. ncbi request reprint Whither withered Golgi? A retrospective evaluation of reticularist and synaptic constructs
    Lawrence Kruger
    Department of Neurobiology, UCLA David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Brain Res Bull 72:201-7. 2007
    ..Both laureates espoused operative principles that have survived in different modes and distinctive temporal domains. Together, they reflect the roots of our contemporary understanding of neural interaction...
  9. ncbi request reprint An ultra small array of electrodes for stimulating multiple inputs into a single neuron
    Spencer L Smith
    Neuroscience and Neuroengineering Programs, 1320 Gonda Center, 695 Young Drive South, University of California, Los Angeles, CA 90095, USA
    J Neurosci Methods 133:109-14. 2004
    ..This device will be useful for exploring spatiotemporal synaptic integration in single neurons. Potential applications in experiments on cerebellar LTD are also discussed...
  10. pmc Isolation of glutamate transport-coupled charge flux and estimation of glutamate uptake at the climbing fiber-Purkinje cell synapse
    Gabor Brasnjo
    Department of Neurobiology, School of Medicine, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 101:6273-8. 2004
    ..Analysis of such synaptic EAAT currents suggests that, on average, postsynaptic EAATs take up approximately 1,300,000 glutamate molecules in response to a single climbing fiber action potential...
  11. pmc Alcohol-induced motor impairment caused by increased extrasynaptic GABA(A) receptor activity
    H Jacob Hanchar
    Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    Nat Neurosci 8:339-45. 2005
    ..These findings identify extrasynaptic GABARs as critical targets underlying low-dose alcohol intoxication and demonstrate that subtle changes in tonic inhibition in one class of neurons can alter behavior...
  12. ncbi request reprint Persistent changes in spontaneous firing of Purkinje neurons triggered by the nitric oxide signaling cascade
    Spencer L Smith
    Department of Neurobiology and Brain Research Institute, University of California at Los Angeles School of Medicine, Los Angeles, California 90095, USA
    J Neurosci 23:367-72. 2003
    ..This mechanism could permit long-term adjustments in the baseline firing rate of endogenously active neurons in response to changes in afferent activity...
  13. pmc Contributions of the GABAA receptor alpha6 subunit to phasic and tonic inhibition revealed by a naturally occurring polymorphism in the alpha6 gene
    Vijayalakshmi Santhakumar
    Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    J Neurosci 26:3357-64. 2006
    ....
  14. pmc Excitation of cerebellar interneurons by group I metabotropic glutamate receptors
    Movses H Karakossian
    Dept of Neurobiology, UCLA Medical Center, 650 Charles Young Drive, Box 951763, Los Angeles, CA 90095 1763, USA
    J Neurophysiol 92:1558-65. 2004
    ..The results also raise the possibility that mGluR1alpha-dependent forms of synaptic plasticity may occur at excitatory inputs to BSNs...
  15. pmc Molecular basis for the high THIP/gaboxadol sensitivity of extrasynaptic GABA(A) receptors
    Pratap Meera
    Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, California, USA
    J Neurophysiol 106:2057-64. 2011
    ..We conclude that δ subunit incorporation into GABARs leads to a dramatic increase in THIP sensitivity, a defining feature that accounts for the unique behavioral and neurophysiological properties of THIP...
  16. pmc Ethanol acts directly on extrasynaptic subtypes of GABAA receptors to increase tonic inhibition
    Vijayalakshmi Santhakumar
    Department of Neurology, David Geffen School of Medicine, University of California, Box 951763, 63 314 CHS, Los Angeles, CA 90095 1763, USA
    Alcohol 41:211-21. 2007
    ..This review evaluates published data for and against a central role of delta subunit-containing GABARs in ethanol actions and suggests future directions that might help settle points of controversy...
  17. pmc Etomidate, propofol and the neurosteroid THDOC increase the GABA efficacy of recombinant alpha4beta3delta and alpha4beta3 GABA A receptors expressed in HEK cells
    Pratap Meera
    Department of Neurobiology, Geffen School of Medicine, University of California, Los Angeles, USA
    Neuropharmacology 56:155-60. 2009
    ..Increasing GABA efficacy on alpha4/6beta3delta and alpha4beta3 receptors is likely to make an important contribution to the anesthetic effects of etomidate, propofol and the neurosteroid THDOC...
  18. ncbi request reprint Comments on "Ethanol sensitivity of GABAergic currents in cerebellar granule neurons is not increased by a single amino acid change (R100Q) in the alpha6 GABA(A) receptor subunit"
    Thomas S Otis
    J Pharmacol Exp Ther 324:399-400; author reply 401-3. 2008
  19. pmc Photochemical control of endogenous ion channels and cellular excitability
    Doris L Fortin
    Department of Molecular and Cell Biology, University of California Berkeley, 121 Life Sciences Addition, Berkeley, California 94720, USA
    Nat Methods 5:331-8. 2008
    ....
  20. ncbi request reprint Blessed are the pacemakers
    Thomas S Otis
    Nat Neurosci 9:297-8. 2006

Research Grants8

  1. EXCITATORY SYNAPTIC SIGNALING BY GLUTAMATE TRANSPORTERS
    Thomas Otis; Fiscal Year: 2004
    ..The findings should also lead to a better understanding of glutamate synapses and provide insight into diseases associated with malfunctions in excitatory transmission such as epilepsy and Amyotrophic Lateral Sclerosis. ..
  2. Protein-based sensors of excitatory synaptic activity
    Thomas Otis; Fiscal Year: 2006
    ..Once developed, such indicators are expected to have an important impact on the study of information processing within neural circuits, allowing minimally invasive measurement of subthreshold synaptic signals. ..
  3. A method for diffraction-limited spot measurements of membrane potential in situ
    Thomas Otis; Fiscal Year: 2009
    ..This proposal presents a noninvasive optical method for measuring neural circuit activity; this novel strategy should enable advancements in our understanding of many of the underlying mechanisms of neurological diseases. ..
  4. A method for diffraction-limited spot measurements of membrane potential in situ
    Thomas S Otis; Fiscal Year: 2010
    ..This proposal presents a noninvasive optical method for measuring neural circuit activity;this novel strategy should enable advancements in our understanding of many of the underlying mechanisms of neurological diseases. ..