Marisa S Otegui
Affiliation: University of Wisconsin
Ding X, Zhang X, Otegui M. Plant autophagy: new flavors on the menu. Curr Opin Plant Biol. 2018;46:113-121 pubmed publisher
..Vacuolar microautophagy, a process by which the vacuole directly engulf cytoplasmic material, also occurs in plants but its underlying molecular mechanisms are yet to be elucidated. ..
Chanoca A, Burkel B, Grotewold E, Eliceiri K, Otegui M. Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM). Methods Mol Biol. 2018;1789:131-141 pubmed publisher
..We discuss here the use of FLIM as a tool for analyzing the subcellular distribution of anthocyanins and estimating variation in vacuolar pH in intact cells. ..
Chanoca A, Kovinich N, Burkel B, Stecha S, Bohorquez Restrepo A, Ueda T, et al
. Anthocyanin Vacuolar Inclusions Form by a Microautophagy Mechanism. Plant Cell. 2015;27:2545-59 pubmed publisher
..We hypothesize that this novel microautophagy mechanism also mediates the transport of other flavonoid aggregates into the vacuole. ..
Otegui M. ESCRT-mediated sorting and intralumenal vesicle concatenation in plants. Biochem Soc Trans. 2018;46:537-545 pubmed publisher
Spitzer C, Li F, Buono R, Roschzttardtz H, Chung T, Zhang M, et al
. The endosomal protein CHARGED MULTIVESICULAR BODY PROTEIN1 regulates the autophagic turnover of plastids in Arabidopsis. Plant Cell. 2015;27:391-402 pubmed publisher
..Consistent with the compromised degradation of plastid proteins, chmp1 plastids show severe morphological defects and aberrant division. We propose that CHMP1 plays a direct role in the autophagic turnover of plastid constituents. ..
Buono R, Paez Valencia J, Miller N, Goodman K, Spitzer C, Spalding E, et al
. Role of SKD1 Regulators LIP5 and IST1-LIKE1 in Endosomal Sorting and Plant Development. Plant Physiol. 2016;171:251-64 pubmed publisher
Buono R, Leier A, Paez Valencia J, Pennington J, Goodman K, Miller N, et al
. ESCRT-mediated vesicle concatenation in plant endosomes. J Cell Biol. 2017;216:2167-2177 pubmed publisher
..Consistent with a potential role as a diffusion barrier, we find that the ESCRT-III protein SNF7 remains associated with ILVs and is delivered to the vacuole for degradation. ..
Otegui M, Noh Y, Martínez D, Vila Petroff M, Staehelin L, Amasino R, et al
. Senescence-associated vacuoles with intense proteolytic activity develop in leaves of Arabidopsis and soybean. Plant J. 2005;41:831-44 pubmed
..The presence of two types of vacuoles in senescing leaves could provide different lytic compartments for the dismantling of specific cellular components. The possible origin and functions of SAVs during leaf senescence are discussed. ..
Otegui M, Herder R, Schulze J, Jung R, Staehelin L. The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies. Plant Cell. 2006;18:2567-81 pubmed
..We hypothesize that the compartmentalized processing of storage proteins in the MVBs may allow for the sequential activation of processing proteases as the MVB lumen gradually acidifies. ..