LAWRENCE OSTROWSKI

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. ncbi request reprint Interferon γ stimulates accumulation of gas phase nitric oxide in differentiated cultures of normal and cystic fibrosis airway epithelial cells
    Lawrence E Ostrowski
    Department of Cell and Developmental Biology, Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, The University of North Carolina, CB 7248, 6123A Thurston Bowles Bldg, Chapel Hill, NC 27599 7248, USA
    Lung 190:563-71. 2012
  2. ncbi request reprint A proteomic analysis of human cilia: identification of novel components
    Lawrence E Ostrowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina, School of Medicine, Chapel Hill, North Carolina 27599 7248, USA
    Mol Cell Proteomics 1:451-65. 2002
  3. ncbi request reprint Targeting expression of a transgene to the airway surface epithelium using a ciliated cell-specific promoter
    Lawrence E Ostrowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Ther 8:637-45. 2003
  4. pmc Cilia and models for studying structure and function
    Lawrence E Ostrowski
    Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA
    Proc Am Thorac Soc 8:423-9. 2011
  5. pmc Conditional deletion of dnaic1 in a murine model of primary ciliary dyskinesia causes chronic rhinosinusitis
    Lawrence E Ostrowski
    The University of North Carolina at Chapel Hill School of Medicine, Cystic Fibrosis Pulmonary Research and Treatment Center, CB 7248, 6123A Thurston Bowles Bldg, Chapel Hill, NC 27599 7248, USA
    Am J Respir Cell Mol Biol 43:55-63. 2010
  6. ncbi request reprint Expression of CFTR from a ciliated cell-specific promoter is ineffective at correcting nasal potential difference in CF mice
    L E Ostrowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Gene Ther 14:1492-501. 2007
  7. pmc Characterization of an A-kinase anchoring protein in human ciliary axonemes
    Patricia L Kultgen
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Biol Cell 13:4156-66. 2002
  8. pmc Voltage-dependent anion channel-1 (VDAC-1) contributes to ATP release and cell volume regulation in murine cells
    Seiko F Okada
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Gen Physiol 124:513-26. 2004
  9. ncbi request reprint Olfactory epithelia exhibit progressive functional and morphological defects in CF mice
    Barbara R Grubb
    CF Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Cell Physiol 293:C574-83. 2007
  10. ncbi request reprint Identification of dynein heavy chain 7 as an inner arm component of human cilia that is synthesized but not assembled in a case of primary ciliary dyskinesia
    Yan J Zhang
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    J Biol Chem 277:17906-15. 2002

Research Grants

Collaborators

Detail Information

Publications14

  1. ncbi request reprint Interferon γ stimulates accumulation of gas phase nitric oxide in differentiated cultures of normal and cystic fibrosis airway epithelial cells
    Lawrence E Ostrowski
    Department of Cell and Developmental Biology, Cystic Fibrosis Pulmonary Research and Treatment Center, School of Medicine, The University of North Carolina, CB 7248, 6123A Thurston Bowles Bldg, Chapel Hill, NC 27599 7248, USA
    Lung 190:563-71. 2012
    ..The objective of these studies was to determine if the low levels of gas phase NO (gNO) could be reproduced in well-differentiated air-liquid interface (ALI) cultures of normal and CF cells...
  2. ncbi request reprint A proteomic analysis of human cilia: identification of novel components
    Lawrence E Ostrowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina, School of Medicine, Chapel Hill, North Carolina 27599 7248, USA
    Mol Cell Proteomics 1:451-65. 2002
    ..These studies represent the first proteomic analysis of the human ciliary axoneme and have identified many potentially novel components of this complex organelle...
  3. ncbi request reprint Targeting expression of a transgene to the airway surface epithelium using a ciliated cell-specific promoter
    Lawrence E Ostrowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Ther 8:637-45. 2003
    ..Our results demonstrate that elements within the FOXJ1 promoter region are sufficient to target expression of transgenes to ciliated cells and may be useful for gene therapy of CF...
  4. pmc Cilia and models for studying structure and function
    Lawrence E Ostrowski
    Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA
    Proc Am Thorac Soc 8:423-9. 2011
    ..Potential therapeutic approaches, based on fundamental knowledge gained from work in these model systems, are also presented...
  5. pmc Conditional deletion of dnaic1 in a murine model of primary ciliary dyskinesia causes chronic rhinosinusitis
    Lawrence E Ostrowski
    The University of North Carolina at Chapel Hill School of Medicine, Cystic Fibrosis Pulmonary Research and Treatment Center, CB 7248, 6123A Thurston Bowles Bldg, Chapel Hill, NC 27599 7248, USA
    Am J Respir Cell Mol Biol 43:55-63. 2010
    ..This model will be useful for the study of the pathogenesis and treatment of PCD...
  6. ncbi request reprint Expression of CFTR from a ciliated cell-specific promoter is ineffective at correcting nasal potential difference in CF mice
    L E Ostrowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Gene Ther 14:1492-501. 2007
    ..However, expression of human CFTR in ciliated cells of the nasal epithelia failed to significantly change the nasal bioelectrics of the CF mice...
  7. pmc Characterization of an A-kinase anchoring protein in human ciliary axonemes
    Patricia L Kultgen
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Biol Cell 13:4156-66. 2002
    ..These data demonstrate that we have identified the first human axonemal AKAP, a protein that likely plays a role in the signaling necessary for efficient modulation of ciliary beat frequency...
  8. pmc Voltage-dependent anion channel-1 (VDAC-1) contributes to ATP release and cell volume regulation in murine cells
    Seiko F Okada
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Gen Physiol 124:513-26. 2004
    ..However, the observation that VDAC-1 knockout cells released a significant amount of ATP suggests that other molecules also play a role in this function...
  9. ncbi request reprint Olfactory epithelia exhibit progressive functional and morphological defects in CF mice
    Barbara R Grubb
    CF Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Am J Physiol Cell Physiol 293:C574-83. 2007
    ..The CF mouse thus provides a novel model to examine the mechanisms of disease-associated loss of olfactory function...
  10. ncbi request reprint Identification of dynein heavy chain 7 as an inner arm component of human cilia that is synthesized but not assembled in a case of primary ciliary dyskinesia
    Yan J Zhang
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    J Biol Chem 277:17906-15. 2002
    ..In cilia from PCD cells, DNAH7 was undetectable, whereas intracellular DNAH7 was clearly present. These studies identify DNAH7 as an inner arm component of human cilia that is synthesized but not assembled in a case of PCD...
  11. ncbi request reprint Investigation of the possible role of a novel gene, DPCD, in primary ciliary dyskinesia
    Maimoona Zariwala
    University of North Carolina at Chapel Hill School of Medicine, Cystic Fibrosis Pulmonary Research and Treatment Center, Chapel Hill, NC 27599 7248, USA
    Am J Respir Cell Mol Biol 30:428-34. 2004
    ..No disease-causing mutations were confirmed; however, one variant could not be excluded. Therefore, DPCD remains a novel candidate gene for PCD...
  12. ncbi request reprint Primary ciliary dyskinesia and upper airway diseases
    Marcus P Kennedy
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, School of Medicine, 6125 Thurston Bowles Building, Chapel Hill, NC 27599, USA
    Curr Allergy Asthma Rep 6:513-7. 2006
    ..Further studies of this disease will likely lead to earlier diagnosis, better treatment, and improved outcomes...
  13. pmc Lung development and repair: contribution of the ciliated lineage
    Emma L Rawlins
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 104:410-7. 2007
    ..From quantitative analysis and confocal microscopy we conclude that ciliated cells transiently change their morphology in response to lung injury but do not proliferate or transdifferentiate as part of the repair process...
  14. ncbi request reprint Real-time analysis of cAMP-mediated regulation of ciliary motility in single primary human airway epithelial cells
    Andreas Schmid
    Division of Pulmonary and Critical Care Medicine, University of Miami School of Medicine, 1600 NW 10th Avenue, RMSB 7063, Miami, FL 33136, USA
    J Cell Sci 119:4176-86. 2006
    ....

Research Grants9

  1. IDENTIFICATION OF BIOCHEMICAL ABNORMALITIES IN PCD CILIA
    LAWRENCE OSTROWSKI; Fiscal Year: 2002
    ..Further studies of the role of these proteins in the assembly and function of cilia may also result in improved treatment for other air way diseases. ..
  2. A Ciliated Cell-Specific Promoter for Gene Therapy of CF
    LAWRENCE OSTROWSKI; Fiscal Year: 2005
    ..Specific Aim 3: To demonstrate correction of the CF phenotype in both in vitro and in vivo models by targeted expression of the normal CFTR gene in ciliated cells. ..
  3. Conditional Deletion of Dnaic1 as a Model of Primary Ciliary Dyskinesia
    LAWRENCE OSTROWSKI; Fiscal Year: 2007
    ..If successful, this model will also provide a novel system to investigate other questions concerning pulmonary health and disease. ..