Fernando A Osorio

Summary

Affiliation: University of Nebraska
Country: USA

Publications

  1. doi request reprint A virulent strain of porcine reproductive and respiratory syndrome virus does not up-regulate interleukin-10 levels in vitro or in vivo
    Sakthivel Subramaniam
    School of Veterinary Medicine and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, NE 68583, USA
    Virus Res 155:415-22. 2011
  2. doi request reprint Identification of amino acid residues important for anti-IFN activity of porcine reproductive and respiratory syndrome virus non-structural protein 1
    Lalit K Beura
    School of Veterinary Medicine and Biomedical Sciences and the Nebraska Center for Virology, University of Nebraska Lincoln, Lincoln, NE 68583 0900, USA
    Virology 433:431-9. 2012
  3. ncbi request reprint Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a truncated form of GP5 and M protein of porcine reproductive and respiratory syndrome virus
    Reginaldo G Bastos
    Department of Veterinary and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, NE, USA
    Vaccine 22:467-74. 2004
  4. doi request reprint Amino acid residues in the non-structural protein 1 of porcine reproductive and respiratory syndrome virus involved in down-regulation of TNF-α expression in vitro and attenuation in vivo
    Sakthivel Subramaniam
    School of Veterinary Medicine and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska Lincoln, NE 68583, USA
    Virology 432:241-9. 2012
  5. doi request reprint Glycosylation of minor envelope glycoproteins of porcine reproductive and respiratory syndrome virus in infectious virus recovery, receptor interaction, and immune response
    Phani B Das
    School of Veterinary Medicine and Biomedical Sciences, and the Nebraska Center for Virology, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0900, USA
    Virology 410:385-94. 2011
  6. ncbi request reprint Infectious clone-derived viruses from virulent and vaccine strains of porcine reproductive and respiratory syndrome virus mimic biological properties of their parental viruses in a pregnant sow model
    Byungjoon Kwon
    Department of Veterinary and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska Lincoln, E126 Beadle Center, 1901 Vine Street, Lincoln, NE 68588, United States
    Vaccine 24:7071-80. 2006
  7. doi request reprint Porcine reproductive and respiratory syndrome virus non-structural protein 1 suppresses tumor necrosis factor-alpha promoter activation by inhibiting NF-κB and Sp1
    Sakthivel Subramaniam
    School of Veterinary Medicine and Biomedical Sciences, University of Nebraska Lincoln, NE 68583, USA
    Virology 406:270-9. 2010
  8. pmc The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163
    Phani B Das
    Department of Veterinary and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0900, USA
    J Virol 84:1731-40. 2010
  9. doi request reprint Development of a porcine reproductive and respiratory syndrome virus differentiable (DIVA) strain through deletion of specific immunodominant epitopes
    Marcelo de Lima
    Nebraska Center for Virology and Department of Veterinary and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0900, USA
    Vaccine 26:3594-600. 2008
  10. pmc Cellular poly(c) binding proteins 1 and 2 interact with porcine reproductive and respiratory syndrome virus nonstructural protein 1β and support viral replication
    Lalit K Beura
    Morrison Research Center, 4240 Fair Street, East Campus, University of Nebraska Lincoln, Lincoln, NE 68583 0900, USA
    J Virol 85:12939-49. 2011

Collaborators

  • Odir A Dellagostin
  • So Young Kwon
  • Z Lu
  • Kyoung Jin Yoon
  • William W Laegreid
  • Eduardo F Flores
  • Asit K Pattnaik
  • Byungjoon Kwon
  • Sakthivel Subramaniam
  • Lalit K Beura
  • Hiep L X Vu
  • Israrul H Ansari
  • Phani B Das
  • Marcelo de Lima
  • Phat X Dinh
  • Jung Hyang Sur
  • Alan R Doster
  • Reginaldo G Bastos
  • Rajeshwari Parida
  • Ana M Jar
  • Ha M Truong
  • Allan R Doster
  • Eric Nelson
  • Robert W Wills
  • Raul G Barletta
  • Federico A Zuckermann
  • Daniel A Peterson
  • In Soo Choi
  • Jonathan L Cooney
  • Clinton Jones
  • Byung Joon Kwon
  • Chi Ho Yu
  • Charles A Kuszynski
  • Ji Young Yhee
  • Saumendra N Sarkar
  • Osvaldo J Lopez
  • Federico Zuckermann
  • Gerald F Kutish
  • Judith Galeota
  • Judith A Galeota

Detail Information

Publications21

  1. doi request reprint A virulent strain of porcine reproductive and respiratory syndrome virus does not up-regulate interleukin-10 levels in vitro or in vivo
    Sakthivel Subramaniam
    School of Veterinary Medicine and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, NE 68583, USA
    Virus Res 155:415-22. 2011
    ..Hence, based on these results obtained with a well-characterized highly pathogenic PRRSV strain it may be concluded that the induction of IL-10 release is not a part of the PRRSV virulence mechanisms...
  2. doi request reprint Identification of amino acid residues important for anti-IFN activity of porcine reproductive and respiratory syndrome virus non-structural protein 1
    Lalit K Beura
    School of Veterinary Medicine and Biomedical Sciences and the Nebraska Center for Virology, University of Nebraska Lincoln, Lincoln, NE 68583 0900, USA
    Virology 433:431-9. 2012
    ..The results indicate a strong selection pressure towards maintaining the IFN-inhibitory property of the virus for successful propagation in pigs...
  3. ncbi request reprint Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a truncated form of GP5 and M protein of porcine reproductive and respiratory syndrome virus
    Reginaldo G Bastos
    Department of Veterinary and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, NE, USA
    Vaccine 22:467-74. 2004
    ..05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection...
  4. doi request reprint Amino acid residues in the non-structural protein 1 of porcine reproductive and respiratory syndrome virus involved in down-regulation of TNF-α expression in vitro and attenuation in vivo
    Sakthivel Subramaniam
    School of Veterinary Medicine and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska Lincoln, NE 68583, USA
    Virology 432:241-9. 2012
    ..In infected pigs, the Nsp1β mutant virus was attenuated in growth. These studies provide insights into how PRRSV evades the effector mechanisms of innate immunity during infection...
  5. doi request reprint Glycosylation of minor envelope glycoproteins of porcine reproductive and respiratory syndrome virus in infectious virus recovery, receptor interaction, and immune response
    Phani B Das
    School of Veterinary Medicine and Biomedical Sciences, and the Nebraska Center for Virology, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0900, USA
    Virology 410:385-94. 2011
    ..These studies reveal the importance of glycosylation of these minor GPs in the biology of PRRSV...
  6. ncbi request reprint Infectious clone-derived viruses from virulent and vaccine strains of porcine reproductive and respiratory syndrome virus mimic biological properties of their parental viruses in a pregnant sow model
    Byungjoon Kwon
    Department of Veterinary and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska Lincoln, E126 Beadle Center, 1901 Vine Street, Lincoln, NE 68588, United States
    Vaccine 24:7071-80. 2006
    ..The development of an infectious clone derived from a bona fide attenuated PRRSV vaccine strain should significantly facilitate ongoing studies to determine the molecular basis of virulence and attenuation...
  7. doi request reprint Porcine reproductive and respiratory syndrome virus non-structural protein 1 suppresses tumor necrosis factor-alpha promoter activation by inhibiting NF-κB and Sp1
    Sakthivel Subramaniam
    School of Veterinary Medicine and Biomedical Sciences, University of Nebraska Lincoln, NE 68583, USA
    Virology 406:270-9. 2010
    ..These findings reveal one of the important mechanisms underlying the innate immune evasion by PRRSV during infection...
  8. pmc The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163
    Phani B Das
    Department of Veterinary and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0900, USA
    J Virol 84:1731-40. 2010
    ....
  9. doi request reprint Development of a porcine reproductive and respiratory syndrome virus differentiable (DIVA) strain through deletion of specific immunodominant epitopes
    Marcelo de Lima
    Nebraska Center for Virology and Department of Veterinary and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0900, USA
    Vaccine 26:3594-600. 2008
    ..In summary, our results provide proof of concept that DIVA PRRSV vaccines can potentially be developed by deletion of individual "marker" immunodominant epitopes...
  10. pmc Cellular poly(c) binding proteins 1 and 2 interact with porcine reproductive and respiratory syndrome virus nonstructural protein 1β and support viral replication
    Lalit K Beura
    Morrison Research Center, 4240 Fair Street, East Campus, University of Nebraska Lincoln, Lincoln, NE 68583 0900, USA
    J Virol 85:12939-49. 2011
    ..Overall, the results presented here point toward an important role for PCBP1 and PCBP2 in regulating PRRSV RNA synthesis...
  11. pmc Immune evasion of porcine reproductive and respiratory syndrome virus through glycan shielding involves both glycoprotein 5 as well as glycoprotein 3
    Hiep L X Vu
    Nebraska Center for Virology and School of Veterinary Medicine and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, NE, USA
    J Virol 85:5555-64. 2011
    ..Collectively, our data demonstrate the involvement of an N-glycan moiety located in GP3 in glycan shield interference...
  12. doi request reprint Identification of virulence determinants of porcine reproductive and respiratory syndrome virus through construction of chimeric clones
    Byungjoon Kwon
    Department of Veterinary and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska Lincoln, 111 K Morrison Life Sc Res Center, 4240 Fair Street, East Campus, Lincoln, NE 68583 0900, USA
    Virology 380:371-8. 2008
    ..Our results allow to conclude that NSP3-8 and ORF5 are the location of major virulence determinants, while other virulence determinants may also be contained in NSP1-3, NSP10-12 and ORF2...
  13. pmc Porcine reproductive and respiratory syndrome virus nonstructural protein 1beta modulates host innate immune response by antagonizing IRF3 activation
    Lalit K Beura
    Nebraska Center for Virology and School of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska 68583, USA
    J Virol 84:1574-84. 2010
    ..We propose that this NSP1beta-mediated subversion of the host innate immune response plays an important role in PRRSV pathogenesis...
  14. pmc Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies
    Israrul H Ansari
    Department of Veterinary and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska Lincoln, Lincoln, Nebraska 68588, USA
    J Virol 80:3994-4004. 2006
    ..These results should have great significance for development of PRRSV vaccines of enhanced protective efficacy...
  15. pmc Distribution and characterization of IL-10-secreting cells in lymphoid tissues of PCV2-infected pigs
    Alan R Doster
    School of Veterinary Medicine and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, Nebraska 68583, USA
    J Vet Sci 11:177-83. 2010
    ..The increased IL-10 expression observed in PCV2 infection in this study suggests that IL-10-mediated immunosuppression may play an important role in the pathogenesis and maintenance of naturally occurring PCV2 infection...
  16. pmc Duration of infection and proportion of pigs persistently infected with porcine reproductive and respiratory syndrome virus
    Robert W Wills
    Department of Veterinary and Biomedical Science, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0907, USA willscvm msstate edu
    J Clin Microbiol 41:58-62. 2003
    ..i. The study's results indicate that most pigs clear PRRSV within 3 to 4 months, but that some may remain persistently infected for several months...
  17. doi request reprint Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus
    Hiep L X Vu
    Nebraska Center for Virology and School of Veterinary Medicine and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, NE 68583, USA
    Vaccine 31:4330-7. 2013
    ..All together, our results provide an important starting point for the development of a live-attenuated DIVA vaccine against type-II PRRSV. ..
  18. doi request reprint Location of T-cell epitopes in nonstructural proteins 9 and 10 of type-II porcine reproductive and respiratory syndrome virus
    Rajeshwari Parida
    Nebraska Center for Virology, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0900, United States
    Virus Res 169:13-21. 2012
    ..The identified epitopes may be important in the formulation of immunogens to provide broad cross-protection against diverse PRRSV strains...
  19. ncbi request reprint A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus
    Ha M Truong
    Department of Veterinary and Biomedical Sciences and the Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68588, USA
    Virology 325:308-19. 2004
    ....
  20. doi request reprint Mouse x pig chimeric antibodies expressed in Baculovirus retain the same properties of their parent antibodies
    Ana M Jar
    Veterinary and Biomedical Sciences Dept, University of Nebraska Lincoln, Lincoln, NE 68583, USA
    Biotechnol Prog 25:516-23. 2009
    ..Expressing chimeric pig antibodies with protective capabilities offers a new alternative strategy for infectious disease control in domestic pigs...
  21. ncbi request reprint Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus
    Reginaldo G Bastos
    Department of Veterinary and Biomedical Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0905, USA
    Vaccine 21:21-9. 2002
    ..These results indicate that BCG was able to express antigens of PRRSV and elicit an immune response against the viral proteins in mice...