Natalia A Osna

Summary

Affiliation: University of Nebraska Medical Center
Country: USA

Publications

  1. pmc Ethanol metabolism alters major histocompatibility complex class I-restricted antigen presentation in liver cells
    Natalia A Osna
    Liver Study Unit, Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
    Hepatology 49:1308-15. 2009
  2. pmc Ethanol and hepatitis C virus suppress peptide-MHC class I presentation in hepatocytes by altering proteasome function
    Natalia A Osna
    Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, Nebraska 68105, USA
    Alcohol Clin Exp Res 36:2028-35. 2012
  3. pmc Involvement of autophagy in alcoholic liver injury and hepatitis C pathogenesis
    Natalia A Osna
    University of Nebraska Medical Center, VA Medical Center, Omaha, NE 68105, United States
    World J Gastroenterol 17:2507-14. 2011
  4. pmc Alcohol and liver, 2010
    Natalia A Osna
    Liver Study Unit, Research Service 151, VA Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, United States
    World J Gastroenterol 16:1303. 2010
  5. pmc Impaired methylation as a novel mechanism for proteasome suppression in liver cells
    Natalia A Osna
    Liver Study Unit, The Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
    Biochem Biophys Res Commun 391:1291-6. 2010
  6. pmc Hepatitis C virus and ethanol alter antigen presentation in liver cells
    Natalia A Osna
    Department of Internal Medicine, University of Nebraska Medical Center, Liver Study Unit R151, VA Medical Center, 4101 Woolworth Ave, Omaha, NE 68105, United States
    World J Gastroenterol 15:1201-8. 2009
  7. pmc Proteasome activation by hepatitis C core protein is reversed by ethanol-induced oxidative stress
    Natalia A Osna
    Liver Study Unit, Omaha Veterans Affairs Medical Center, Omaha, Nebraska 68105, USA
    Gastroenterology 134:2144-52. 2008
  8. ncbi request reprint Implication of altered proteasome function in alcoholic liver injury
    Natalia A Osna
    Liver Study Unit, Research Service 151, VA Medical Center, 4101 Woolworth Ave, Omaha, NE 68105, USA
    World J Gastroenterol 13:4931-7. 2007
  9. ncbi request reprint Ethanol-induced oxidative stress suppresses generation of peptides for antigen presentation by hepatoma cells
    Natalia A Osna
    Liver Study Unit, The Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
    Hepatology 45:53-61. 2007
  10. ncbi request reprint Peroxynitrite alters the catalytic activity of rodent liver proteasome in vitro and in vivo
    Natalia A Osna
    Liver Study Unit, The Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
    Hepatology 40:574-82. 2004

Research Grants

Detail Information

Publications26

  1. pmc Ethanol metabolism alters major histocompatibility complex class I-restricted antigen presentation in liver cells
    Natalia A Osna
    Liver Study Unit, Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
    Hepatology 49:1308-15. 2009
    ..However, in primary hepatocytes, even in the absence of IFNgamma, we observed a similar decline in proteasome activity and antigen presentation after ethanol exposure...
  2. pmc Ethanol and hepatitis C virus suppress peptide-MHC class I presentation in hepatocytes by altering proteasome function
    Natalia A Osna
    Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, Nebraska 68105, USA
    Alcohol Clin Exp Res 36:2028-35. 2012
    ..Here, we examined the effects of EtOH consumption by HCV core transgenic mice on proteasome activity in hepatocytic lysates and in partially purified 26S proteasome and the impact of these changes on antigen presentation...
  3. pmc Involvement of autophagy in alcoholic liver injury and hepatitis C pathogenesis
    Natalia A Osna
    University of Nebraska Medical Center, VA Medical Center, Omaha, NE 68105, United States
    World J Gastroenterol 17:2507-14. 2011
    ..Because ethanol induces autophagosome expression, enhanced levels of autophagic proteins may enhance HCV infectivity in liver cells of alcoholics and heavy drinkers...
  4. pmc Alcohol and liver, 2010
    Natalia A Osna
    Liver Study Unit, Research Service 151, VA Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, United States
    World J Gastroenterol 16:1303. 2010
    ..These extrahepatic manifestations are regulated via the similar hepatitis mechanisms. In the Topic Highlight series, we provide an update of current knowledge in the field of ALD...
  5. pmc Impaired methylation as a novel mechanism for proteasome suppression in liver cells
    Natalia A Osna
    Liver Study Unit, The Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
    Biochem Biophys Res Commun 391:1291-6. 2010
    ..We conclude that impaired methylation of proteasome subunits suppressed proteasome activity in liver cells indicating an additional, yet novel mechanism of proteasome activity regulation by ethanol...
  6. pmc Hepatitis C virus and ethanol alter antigen presentation in liver cells
    Natalia A Osna
    Department of Internal Medicine, University of Nebraska Medical Center, Liver Study Unit R151, VA Medical Center, 4101 Woolworth Ave, Omaha, NE 68105, United States
    World J Gastroenterol 15:1201-8. 2009
    ..Collectively, ethanol exposure further impairs antigen presentation in HCV-infected liver cells, which may provide a partial explanation for exacerbations and the poor outcome of HCV infection in alcoholics...
  7. pmc Proteasome activation by hepatitis C core protein is reversed by ethanol-induced oxidative stress
    Natalia A Osna
    Liver Study Unit, Omaha Veterans Affairs Medical Center, Omaha, Nebraska 68105, USA
    Gastroenterology 134:2144-52. 2008
    ..The aim was to investigate whether core protein, by its ability to generate oxidants, alters proteasome activity and whether these alterations are further affected by ethanol exposure...
  8. ncbi request reprint Implication of altered proteasome function in alcoholic liver injury
    Natalia A Osna
    Liver Study Unit, Research Service 151, VA Medical Center, 4101 Woolworth Ave, Omaha, NE 68105, USA
    World J Gastroenterol 13:4931-7. 2007
    ..Finally, ethanol-elicited blockade of interferon type 1 and 2 signaling and decreased proteasome function impairs generation of peptides for MHC class I-restricted antigen presentation...
  9. ncbi request reprint Ethanol-induced oxidative stress suppresses generation of peptides for antigen presentation by hepatoma cells
    Natalia A Osna
    Liver Study Unit, The Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
    Hepatology 45:53-61. 2007
    ..Treatment of cells with interferon gamma, which enhances proteasome activity, did not reverse the effects of ethanol. Ethanol exerted similar effects on WIFB cells, indicating that its effects are not unique to one cell type...
  10. ncbi request reprint Peroxynitrite alters the catalytic activity of rodent liver proteasome in vitro and in vivo
    Natalia A Osna
    Liver Study Unit, The Omaha Veterans Affairs VA Medical Center, Omaha, NE 68105, USA
    Hepatology 40:574-82. 2004
    ..In conclusion, PN dose-dependently modulated proteasome activity, regulating protein degradation by the proteasome in liver cells...
  11. ncbi request reprint Ethanol metabolism alters interferon gamma signaling in recombinant HepG2 cells
    Natalia A Osna
    Liver Study Unit, Research Service, The Omaha Veterans Affairs VA Medical Center, University of Nebraska Medical Center, Omaha, NE 68105, USA
    Hepatology 42:1109-17. 2005
    ..In conclusion, under conditions of ethanol-elicited oxidative stress, PN prevents STAT1 phosphorylation by stabilization of SOCS1, and possibly by nitration of tyrosine residues in STAT1 protein...
  12. doi request reprint Chronic ethanol consumption results in atypical liver injury in copper/zinc superoxide dismutase deficient mice
    Tiana V Curry-McCoy
    Liver Study Unit, Omaha Veterans Affairs Medical Center, University of Nebraska Medical Center, Omaha, Nebraska, USA
    Alcohol Clin Exp Res 34:251-61. 2010
    ..Because the absence of SOD would confer less resistance to oxidative stress, we determined whether damage to hepatic proteolytic systems was greater in SOD(-/-) than in SOD(+/+) mice after chronic ethanol feeding...
  13. ncbi request reprint Lysosomal leakage and lack of adaptation of hepatoprotective enzyme contribute to enhanced susceptibility to ethanol-induced liver injury in female rats
    Terrence M Donohue
    Liver Study Unit, VA Nebraska, Western Iowa Health Care Network, Omaha, Nebraska 68105, USA
    Alcohol Clin Exp Res 31:1944-52. 2007
    ..Here, we determined whether differential liver injury occurred in male and female rats after feeding these animals liquid diets containing either ethanol or isocaloric dextrose with fish oil as the sole source of lipid...
  14. pmc Impact of altered methylation in cytokine signaling and proteasome function in alcohol and viral-mediated diseases
    Kusum K Kharbanda
    Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, NE 68105, USA
    Alcohol Clin Exp Res 37:1-7. 2013
    ..Two therapeutic modalities, betaine and S-adenosylmethionine, can correct methylation defects to attenuate many EtOH-induced liver changes, as well as improve IFN signaling pathways, thereby overcoming viral treatment resistance...
  15. ncbi request reprint Recombinant Hep G2 cells that express alcohol dehydrogenase and cytochrome P450 2E1 as a model of ethanol-elicited cytotoxicity
    Terrence M Donohue
    Liver Study Unit, VA Medical Center, Omaha, NE 68105, USA
    Int J Biochem Cell Biol 38:92-101. 2006
    ..Furthermore, the metabolism of ethanol by these cells via ADH and CYP2E1 is sufficient to bring about an inhibition of proteasome activity that may lead to apoptotic cell death...
  16. pmc Early growth response-1 contributes to steatosis development after acute ethanol administration
    Terrence M Donohue
    Liver Study Unit, Nebraska Western Iowa Health Care System, College of Medicine, University of Nebraska Medical Center, Omaha, USA
    Alcohol Clin Exp Res 36:759-67. 2012
    ..Here, we determined the extent to which Egr-1 is involved in fatty liver development in mice subjected to acute EtOH administration...
  17. ncbi request reprint Role of the proteasome in ethanol-induced liver pathology
    Terrence M Donohue
    Liver Study Unit, Omaha VA Medical Center, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA
    Alcohol Clin Exp Res 31:1446-59. 2007
    ....
  18. pmc Cellular steatosis in ethanol oxidizing-HepG2 cells is partially controlled by the transcription factor, early growth response-1
    Paul G Thomes
    VA Nebraska Western Iowa Health Care System, Omaha, NE 68105, USA
    Int J Biochem Cell Biol 45:454-63. 2013
    ..Double knockdown of both Egr-1 and SREBP-1c abolished ethanol-elicited steatosis. Collectively, our findings provide important new insights into the temporal regulation by ethanol oxidation of Egr-1 and cellular steatosis...
  19. ncbi request reprint Interferon gamma enhances proteasome activity in recombinant Hep G2 cells that express cytochrome P4502E1: modulation by ethanol
    Natalia A Osna
    Liver Study Unit, Research Service 151, The Veterans Affairs Medical Center, University of Nebraska Medical Center, 4101 Woolworth Ave, Omaha, NE 68105 8090, USA
    Biochem Pharmacol 66:697-710. 2003
    ..Ethanol metabolism by VL-17A cells suppresses IFNgamma-mediated induction of proteasome activity, in part, by preventing STAT1 phosphorylation...
  20. ncbi request reprint L-Buthionine (S,R) sulfoximine depletes hepatic glutathione but protects against ethanol-induced liver injury
    Terrence M Donohue
    Liver Study Unit, U S Department of Veterans Affairs Medical Center, University of Nebraska Medical Center, Omaha, Nebraska 68105, USA
    Alcohol Clin Exp Res 31:1053-60. 2007
    ..Here we investigated whether treatment with BSO enhanced ethanol-induced liver injury in mice...
  21. doi request reprint Alcohol consumption decreases rat hepatic creatine biosynthesis via altered guanidinoacetate methyltransferase activity
    Kusum K Kharbanda
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska Research Service, Veterans Affairs Nebraska Western Iowa Health Care System, Omaha, Nebraska Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska
    Alcohol Clin Exp Res 38:641-8. 2014
    ..As GAMT is the major utilizer of SAM, the purpose of the study was to examine the effects of ethanol (EtOH) on liver creatine levels and GAMT activity...
  22. doi request reprint Susceptibility to T cell-mediated liver injury is enhanced in asialoglycoprotein receptor-deficient mice
    Benita L McVicker
    Research Service 151, VA Nebraska Western Iowa Health Care System, Omaha, NE 68105, United States
    Int Immunopharmacol 16:17-26. 2013
    ....
  23. pmc Betaine treatment attenuates chronic ethanol-induced hepatic steatosis and alterations to the mitochondrial respiratory chain proteome
    Kusum K Kharbanda
    Research Service 151, Veterans Affairs Nebraska Western Iowa Health Care System, 4101 Woolworth Avenue, Omaha, NE 68105, USA
    Int J Hepatol 2012:962183. 2012
    ..Therefore, preservation of mitochondrial function may be another key molecular mechanism responsible for betaine hepatoprotection...
  24. pmc Modulation of lysozyme function and degradation after nitration with peroxynitrite
    Tiana V Curry-McCoy
    Liver Study Unit, Omaha Veterans Affairs Medical Center, USA
    Biochim Biophys Acta 1790:778-86. 2009
    ..Here, we investigated PN-induced alterations in lysozyme structure and function, and whether they altered the protein's susceptibility to proteasome-catalyzed degradation...
  25. doi request reprint CYP2E1-Catalyzed Alcohol Metabolism: Role of Oxidant Generation in Interferon Signaling, Antigen Presentation and Autophagy
    Natalia A Osna
    Department of Veterans Affairs, VA Nebraska Western Iowa Health Care System, Omaha VA Medical Center, Omaha, NE, USA
    Subcell Biochem 67:177-97. 2013
    ..Here, we describe our own and other published work relevant to the importance of CYP2E1-catalyzed ethanol oxidation and how this catalysis affects the aforementioned cellular processes to produce liver injury...

Research Grants1

  1. Ethanol Effects on Antigen Presentation in Liver Cells
    Natalia Osna; Fiscal Year: 2006
    ..The results derived from this study will clarify the link between the effects of ethanol on antigen presentation by liver cells, altered immune response and the possible mechanisms of alcohol-related progression of viral hepatitis. ..