Research Topics
Genomes and Genes | Akira OnoSummaryAffiliation: University of Michigan Country: USA Publications
Research Grants
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Publications
Association of human immunodeficiency virus type 1 gag with membrane does not require highly basic sequences in the nucleocapsid: use of a novel Gag multimerization assayAkira Ono
Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, Maryland, USA
J Virol 79:14131-40. 2005..This report offers new insights into the association of HIV-1 Gag with membrane and with lipid rafts...
[Subcellular locations at which HIV-1 assembles]Akira Ono
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
Uirusu 57:9-18. 2007....- HIV-1 assembly at the plasma membraneAkira Ono
Department of Microbiology and Immunology, University of Michigan Medical School, 5736 Medical Science Building II, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0620, United States
Vaccine 28:B55-9. 2010..Recent work by our lab and others started to reveal a molecular mechanism by which HIV ensures to make the plasma membrane as a primary site of virus assembly... - Relationships between plasma membrane microdomains and HIV-1 assemblyAkira Ono
Department of Microbiology and Immunology, University of Michigan Medical School, 5736 Medical Science Building II, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0620, USA
Biol Cell 102:335-50. 2010..Potential roles played by microdomains will be discussed with regard to two post-assembly events, i.e., inhibition of virus release by a raft-associated protein BST-2/tetherin and cell-to-cell HIV-1 transmission at virological synapses... - Depletion of cellular cholesterol inhibits membrane binding and higher-order multimerization of human immunodeficiency virus type 1 GagAkira Ono
Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
Virology 360:27-35. 2007..Altogether, these results are consistent with the hypothesis that cholesterol-enriched membrane microdomains promote HIV-1 particle production by facilitating both Gag-membrane binding and Gag multimerization... - Inhibition of human immunodeficiency virus type 1 assembly and release by the cholesterol-binding compound amphotericin B methyl ester: evidence for Vpu dependenceAbdul A Waheed
Virus Cell Interaction Section, HIV Drug Resistance Program, NCI Frederick, Bldg 535, RM 108, Frederick, MD 21702 1201, USA
J Virol 82:9776-81. 2008..We demonstrated that the ability of Vpu to counter the activity of CD317/BST-2/tetherin is markedly reduced by AME. These results indicate that AME interferes with the anti-CD317/BST-2/tetherin function of Vpu... - Quantitative fluorescence resonance energy transfer microscopy analysis of the human immunodeficiency virus type 1 Gag-Gag interaction: relative contributions of the CA and NC domains and membrane bindingIan B Hogue
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
J Virol 83:7322-36. 2009..These results from cell-based experiments suggest a model in which both membrane binding and NC-RNA interactions serve similar scaffolding functions so that one can functionally compensate for a defect in the other... - Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membraneIan B Hogue
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
J Virol 85:9749-66. 2011..Together, these results suggest that different membrane microdomain components are recruited in a stepwise manner during assembly... 
Post-digestion ¹⁸O exchange/labeling for quantitative shotgun proteomics of membrane proteinsXiaoying Ye
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
Methods Mol Biol 893:223-40. 2012..Sample losses are minimized because solubilization, digestion, and stable isotope labeling are carried out in a single tube, avoiding any sample transfer or buffer exchange between these steps...- Real-time visualization of HIV-1 GAG trafficking in infected macrophagesKarine Gousset
Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, Maryland, USA
PLoS Pathog 4:e1000015. 2008..These data indicate that a population of Gag in infected macrophages remains sequestered internally and is presented to uninfected target cells at a virological synapse... - Interaction between the human immunodeficiency virus type 1 Gag matrix domain and phosphatidylinositol-(4,5)-bisphosphate is essential for efficient gag membrane bindingVineela Chukkapalli
Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Dr, Room 5736A, Ann Arbor, MI 48109, USA
J Virol 82:2405-17. 2008..Altogether, these results indicate that HIV-1 Gag binds PI(4,5)P(2) on the membrane and that the MA basic domain mediates this interaction... - Dynamic Association between HIV-1 Gag and Membrane DomainsIan B Hogue
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Mol Biol Int 2012:979765. 2012..Relationships between Gag multimerization and microdomain association will be further discussed in the context of Gag localization to T-cell uropods and virological synapses... - Opposing mechanisms involving RNA and lipids regulate HIV-1 Gag membrane binding through the highly basic region of the matrix domainVineela Chukkapalli
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Proc Natl Acad Sci U S A 107:1600-5. 2010.... - Phosphatidylinositol (4,5) bisphosphate regulates HIV-1 Gag targeting to the plasma membraneAkira Ono
Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702 1201, USA
Proc Natl Acad Sci U S A 101:14889-94. 2004..These results demonstrate that PI(4,5)P2 plays a key role in Gag targeting to the plasma membrane and thus serves as a cellular determinant of HIV-1 particle production... - Nucleocapsid promotes localization of HIV-1 gag to uropods that participate in virological synapses between T cellsG Nicholas Llewellyn
Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
PLoS Pathog 6:e1001167. 2010..Taken together, these results support a model in which NC-dependent Gag accumulation to uropods establishes a preformed platform that later constitutes T-cell-T-cell contacts at which HIV-1 virus transfer occurs... - Assembly and replication of HIV-1 in T cells with low levels of phosphatidylinositol-(4,5)-bisphosphateKazuaki Monde
Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Dr, Ann Arbor, MI 48109, USA
J Virol 85:3584-95. 2011..In T cells with low PI(4,5)P(2) levels, however, the reduced virus particle production can be compensated for by a mutation that enhances virus infectivity... - Human endogenous retrovirus K Gag coassembles with HIV-1 Gag and reduces the release efficiency and infectivity of HIV-1Kazuaki Monde
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
J Virol 86:11194-208. 2012..Altogether, these results indicate that Gag proteins of endogenous retroviruses can coassemble with HIV-1 Gag and modulate the late phase of HIV-1 replication... - Molecular determinants that regulate plasma membrane association of HIV-1 GagVineela Chukkapalli
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
J Mol Biol 410:512-24. 2011.... - Methods for the study of HIV-1 assemblyAbdul A Waheed
Virus Cell Interaction Section, HIV Drug Resistance Program NCI Frederick, National Institutes of Health, Frederick MD, USA
Methods Mol Biol 485:163-84. 2009..Techniques have been developed in many laboratories to study each of the distinct phases of the HIV-1 assembly and release pathway. A number of these techniques are described in detail in this chapter... - Roles played by capsid-dependent induction of membrane curvature and Gag-ESCRT interactions in tetherin recruitment to HIV-1 assembly sitesJonathan R Grover
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
J Virol 87:4650-64. 2013..These results support a model in which both Gag-induced membrane curvature and Gag-ESCRT interactions promote tetherin recruitment, but the recruitment level achieved by the former is sufficient for full restriction... - Gag localization and virus-like particle release mediated by the matrix domain of human T-lymphotropic virus type 1 Gag are less dependent on phosphatidylinositol-(4,5)-bisphosphate than those mediated by the matrix domain of HIV-1 GagJingga Inlora
Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Dr, Ann Arbor, MI 48109, USA
J Virol 85:3802-10. 2011..Altogether, our data suggest that Gag targeting and membrane binding mediated by HTLV-1 MA does not require PI(4,5)P(2) and that distinct mechanisms regulate HIV-1 and HTLV-1 Gag membrane binding... - Role of lipid rafts in virus replicationAkira Ono
Virus-Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, National Institutes of Health, Maryland 21702, USA
Adv Virus Res 64:311-58. 2005 - Human apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) is incorporated into HIV-1 virions through interactions with viral and nonviral RNAsEvguenia S Svarovskaia
HIV Drug Resistance Program and AIDS Vaccine Program, Science Applications International Corporation Frederick, Inc, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
J Biol Chem 279:35822-8. 2004.... - HIV-1 Gag Associates with Specific Uropod-Directed Microdomains in a Manner Dependent on Its MA Highly Basic RegionG Nicholas Llewellyn
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
J Virol 87:6441-54. 2013..Taken totogether, these findings revealed that HIV-1 Gag associates with specific microdomains present in polarized T cells in an MA-dependent manner, which results in modification of the microdomain constituents... - Optimized method for computing (18)O/(16)O ratios of differentially stable-isotope labeled peptides in the context of postdigestion (18)O exchange/labelingXiaoying Ye
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Maryland 21702, USA
Anal Chem 82:5878-86. 2010.... - Dominant negative inhibition of human immunodeficiency virus particle production by the nonmyristoylated form of gagShigeo Kawada
Kitasato Institute for Life Sciences and Graduate School for Infection Control, Kitasato University, Shirokane 5 9 1, Minato ku, Tokyo 108 8641, Japan
J Virol 82:4384-99. 2008..Overall, our data indicate that coassembly with the nonmyristoylated Gag impairs HIV particle release, a phenomenon that may involve NC-mediated Gag-Gag interaction... - Cell-type-dependent targeting of human immunodeficiency virus type 1 assembly to the plasma membrane and the multivesicular bodyAkira Ono
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
J Virol 78:1552-63. 2004..These data are consistent with a model for Gag targeting that postulates two different cellular binding partners for Gag, one on the plasma membrane and the other in the MVB... - Defects in human immunodeficiency virus budding and endosomal sorting induced by TSG101 overexpressionRitu Goila-Gaur
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
J Virol 77:6507-19. 2003..These results highlight the importance of TSG101 and the endosomal sorting pathway in virus budding and suggest that inhibitors can be developed that, like TSG-5', target HIV-1 without disrupting endosomal sorting... - Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain functionDimiter G Demirov
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
Proc Natl Acad Sci U S A 99:955-60. 2002..These data demonstrate a link between the E2-like domain of TSG101 and HIV-1 L domain function, and indicate that TSG101 derivatives can act as potent and specific inhibitors of HIV-1 replication by blocking virus budding... - Sliding movements of molluscan and algal myosin attached to a magnetizable bead under a load controlled by electromagnetTakashi Watari
Department of Biology, Faculty of Science, Kobe University, Kobe, 657-8501 Japan
J Physiol Sci 56:13-20. 2006.... - Pravastatin does not have a consistent antiviral effect in chronically HIV-infected individuals on antiretroviral therapyPeter A Sklar
AIDS 19:1109-11. 2005
Research Grants
- Mechanisms that determine subcellular sites of HIV-1 assemblyAkira Ono; Fiscal Year: 2010..The experiments proposed here will determine the roles played by PI(4,5)P2 in HIV-1 particle production and will contribute to the future development of antiviral drugs that target HIV-1 assembly and release. ..
- Mechanisms that determine subcellular sites of HIV-1 assemblyAkira Ono; Fiscal Year: 2010..The experiments proposed here will determine the roles played by PI(4,5)P2 in HIV-1 particle production and will contribute to the future development of antiviral drugs that target HIV-1 assembly and release. ..