Akira Ono

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. pmc Nucleocapsid promotes localization of HIV-1 gag to uropods that participate in virological synapses between T cells
    G Nicholas Llewellyn
    Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS Pathog 6:e1001167. 2010
  2. pmc Dynamic Association between HIV-1 Gag and Membrane Domains
    Ian B Hogue
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Biol Int 2012:979765. 2012
  3. pmc Depletion of cellular cholesterol inhibits membrane binding and higher-order multimerization of human immunodeficiency virus type 1 Gag
    Akira Ono
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Virology 360:27-35. 2007
  4. pmc Association of human immunodeficiency virus type 1 gag with membrane does not require highly basic sequences in the nucleocapsid: use of a novel Gag multimerization assay
    Akira Ono
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, Maryland, USA
    J Virol 79:14131-40. 2005
  5. ncbi request reprint [Subcellular locations at which HIV-1 assembles]
    Akira Ono
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    Uirusu 57:9-18. 2007
  6. pmc HIV-1 assembly at the plasma membrane
    Akira Ono
    Department of Microbiology and Immunology, University of Michigan Medical School, 5736 Medical Science Building II, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0620, United States
    Vaccine 28:B55-9. 2010
  7. pmc Relationships between plasma membrane microdomains and HIV-1 assembly
    Akira Ono
    Department of Microbiology and Immunology, University of Michigan Medical School, 5736 Medical Science Building II, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0620, USA
    Biol Cell 102:335-50. 2010
  8. pmc Inhibition of human immunodeficiency virus type 1 assembly and release by the cholesterol-binding compound amphotericin B methyl ester: evidence for Vpu dependence
    Abdul A Waheed
    Virus Cell Interaction Section, HIV Drug Resistance Program, NCI Frederick, Bldg 535, RM 108, Frederick, MD 21702 1201, USA
    J Virol 82:9776-81. 2008
  9. pmc Real-time visualization of HIV-1 GAG trafficking in infected macrophages
    Karine Gousset
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, Maryland, USA
    PLoS Pathog 4:e1000015. 2008
  10. pmc Quantitative fluorescence resonance energy transfer microscopy analysis of the human immunodeficiency virus type 1 Gag-Gag interaction: relative contributions of the CA and NC domains and membrane binding
    Ian B Hogue
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Virol 83:7322-36. 2009

Collaborators

Detail Information

Publications33

  1. pmc Nucleocapsid promotes localization of HIV-1 gag to uropods that participate in virological synapses between T cells
    G Nicholas Llewellyn
    Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS Pathog 6:e1001167. 2010
    ..Taken together, these results support a model in which NC-dependent Gag accumulation to uropods establishes a preformed platform that later constitutes T-cell-T-cell contacts at which HIV-1 virus transfer occurs...
  2. pmc Dynamic Association between HIV-1 Gag and Membrane Domains
    Ian B Hogue
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Biol Int 2012:979765. 2012
    ..Relationships between Gag multimerization and microdomain association will be further discussed in the context of Gag localization to T-cell uropods and virological synapses...
  3. pmc Depletion of cellular cholesterol inhibits membrane binding and higher-order multimerization of human immunodeficiency virus type 1 Gag
    Akira Ono
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Virology 360:27-35. 2007
    ..Altogether, these results are consistent with the hypothesis that cholesterol-enriched membrane microdomains promote HIV-1 particle production by facilitating both Gag-membrane binding and Gag multimerization...
  4. pmc Association of human immunodeficiency virus type 1 gag with membrane does not require highly basic sequences in the nucleocapsid: use of a novel Gag multimerization assay
    Akira Ono
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, Maryland, USA
    J Virol 79:14131-40. 2005
    ..This report offers new insights into the association of HIV-1 Gag with membrane and with lipid rafts...
  5. ncbi request reprint [Subcellular locations at which HIV-1 assembles]
    Akira Ono
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    Uirusu 57:9-18. 2007
    ....
  6. pmc HIV-1 assembly at the plasma membrane
    Akira Ono
    Department of Microbiology and Immunology, University of Michigan Medical School, 5736 Medical Science Building II, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0620, United States
    Vaccine 28:B55-9. 2010
    ..Recent work by our lab and others started to reveal a molecular mechanism by which HIV ensures to make the plasma membrane as a primary site of virus assembly...
  7. pmc Relationships between plasma membrane microdomains and HIV-1 assembly
    Akira Ono
    Department of Microbiology and Immunology, University of Michigan Medical School, 5736 Medical Science Building II, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0620, USA
    Biol Cell 102:335-50. 2010
    ..Potential roles played by microdomains will be discussed with regard to two post-assembly events, i.e., inhibition of virus release by a raft-associated protein BST-2/tetherin and cell-to-cell HIV-1 transmission at virological synapses...
  8. pmc Inhibition of human immunodeficiency virus type 1 assembly and release by the cholesterol-binding compound amphotericin B methyl ester: evidence for Vpu dependence
    Abdul A Waheed
    Virus Cell Interaction Section, HIV Drug Resistance Program, NCI Frederick, Bldg 535, RM 108, Frederick, MD 21702 1201, USA
    J Virol 82:9776-81. 2008
    ..We demonstrated that the ability of Vpu to counter the activity of CD317/BST-2/tetherin is markedly reduced by AME. These results indicate that AME interferes with the anti-CD317/BST-2/tetherin function of Vpu...
  9. pmc Real-time visualization of HIV-1 GAG trafficking in infected macrophages
    Karine Gousset
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, Maryland, USA
    PLoS Pathog 4:e1000015. 2008
    ..These data indicate that a population of Gag in infected macrophages remains sequestered internally and is presented to uninfected target cells at a virological synapse...
  10. pmc Quantitative fluorescence resonance energy transfer microscopy analysis of the human immunodeficiency virus type 1 Gag-Gag interaction: relative contributions of the CA and NC domains and membrane binding
    Ian B Hogue
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Virol 83:7322-36. 2009
    ..These results from cell-based experiments suggest a model in which both membrane binding and NC-RNA interactions serve similar scaffolding functions so that one can functionally compensate for a defect in the other...
  11. pmc Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane
    Ian B Hogue
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Virol 85:9749-66. 2011
    ..Together, these results suggest that different membrane microdomain components are recruited in a stepwise manner during assembly...
  12. doi request reprint Post-digestion ¹⁸O exchange/labeling for quantitative shotgun proteomics of membrane proteins
    Xiaoying Ye
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 893:223-40. 2012
    ..Sample losses are minimized because solubilization, digestion, and stable isotope labeling are carried out in a single tube, avoiding any sample transfer or buffer exchange between these steps...
  13. pmc Roles played by capsid-dependent induction of membrane curvature and Gag-ESCRT interactions in tetherin recruitment to HIV-1 assembly sites
    Jonathan R Grover
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    J Virol 87:4650-64. 2013
    ..These results support a model in which both Gag-induced membrane curvature and Gag-ESCRT interactions promote tetherin recruitment, but the recruitment level achieved by the former is sufficient for full restriction...
  14. pmc Interaction between the human immunodeficiency virus type 1 Gag matrix domain and phosphatidylinositol-(4,5)-bisphosphate is essential for efficient gag membrane binding
    Vineela Chukkapalli
    Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Dr, Room 5736A, Ann Arbor, MI 48109, USA
    J Virol 82:2405-17. 2008
    ..Altogether, these results indicate that HIV-1 Gag binds PI(4,5)P(2) on the membrane and that the MA basic domain mediates this interaction...
  15. pmc Phosphatidylinositol (4,5) bisphosphate regulates HIV-1 Gag targeting to the plasma membrane
    Akira Ono
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702 1201, USA
    Proc Natl Acad Sci U S A 101:14889-94. 2004
    ..These results demonstrate that PI(4,5)P2 plays a key role in Gag targeting to the plasma membrane and thus serves as a cellular determinant of HIV-1 particle production...
  16. pmc Opposing mechanisms involving RNA and lipids regulate HIV-1 Gag membrane binding through the highly basic region of the matrix domain
    Vineela Chukkapalli
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 107:1600-5. 2010
    ....
  17. pmc HIV-1 Gag associates with specific uropod-directed microdomains in a manner dependent on its MA highly basic region
    G Nicholas Llewellyn
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    J Virol 87:6441-54. 2013
    ..Taken together, these findings revealed that HIV-1 Gag associates with specific microdomains present in polarized T cells in an MA-dependent manner, which results in modification of the microdomain constituents...
  18. pmc Evidence in support of RNA-mediated inhibition of phosphatidylserine-dependent HIV-1 Gag membrane binding in cells
    Vineela Chukkapalli
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    J Virol 87:7155-9. 2013
    ....
  19. pmc Assembly and replication of HIV-1 in T cells with low levels of phosphatidylinositol-(4,5)-bisphosphate
    Kazuaki Monde
    Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Dr, Ann Arbor, MI 48109, USA
    J Virol 85:3584-95. 2011
    ..In T cells with low PI(4,5)P(2) levels, however, the reduced virus particle production can be compensated for by a mutation that enhances virus infectivity...
  20. pmc Human endogenous retrovirus K Gag coassembles with HIV-1 Gag and reduces the release efficiency and infectivity of HIV-1
    Kazuaki Monde
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    J Virol 86:11194-208. 2012
    ..Altogether, these results indicate that Gag proteins of endogenous retroviruses can coassemble with HIV-1 Gag and modulate the late phase of HIV-1 replication...
  21. pmc Molecular determinants that regulate plasma membrane association of HIV-1 Gag
    Vineela Chukkapalli
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Mol Biol 410:512-24. 2011
    ....
  22. doi request reprint Methods for the study of HIV-1 assembly
    Abdul A Waheed
    Virus Cell Interaction Section, HIV Drug Resistance Program NCI Frederick, National Institutes of Health, Frederick MD, USA
    Methods Mol Biol 485:163-84. 2009
    ..Techniques have been developed in many laboratories to study each of the distinct phases of the HIV-1 assembly and release pathway. A number of these techniques are described in detail in this chapter...
  23. pmc Gag localization and virus-like particle release mediated by the matrix domain of human T-lymphotropic virus type 1 Gag are less dependent on phosphatidylinositol-(4,5)-bisphosphate than those mediated by the matrix domain of HIV-1 Gag
    Jingga Inlora
    Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Dr, Ann Arbor, MI 48109, USA
    J Virol 85:3802-10. 2011
    ..Altogether, our data suggest that Gag targeting and membrane binding mediated by HTLV-1 MA does not require PI(4,5)P(2) and that distinct mechanisms regulate HIV-1 and HTLV-1 Gag membrane binding...
  24. ncbi request reprint Role of lipid rafts in virus replication
    Akira Ono
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, National Institutes of Health, Maryland 21702, USA
    Adv Virus Res 64:311-58. 2005
  25. ncbi request reprint Human apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) is incorporated into HIV-1 virions through interactions with viral and nonviral RNAs
    Evguenia S Svarovskaia
    HIV Drug Resistance Program and AIDS Vaccine Program, Science Applications International Corporation Frederick, Inc, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Biol Chem 279:35822-8. 2004
    ....
  26. pmc Optimized method for computing (18)O/(16)O ratios of differentially stable-isotope labeled peptides in the context of postdigestion (18)O exchange/labeling
    Xiaoying Ye
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Maryland 21702, USA
    Anal Chem 82:5878-86. 2010
    ....
  27. pmc Defects in human immunodeficiency virus budding and endosomal sorting induced by TSG101 overexpression
    Ritu Goila-Gaur
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Virol 77:6507-19. 2003
    ..These results highlight the importance of TSG101 and the endosomal sorting pathway in virus budding and suggest that inhibitors can be developed that, like TSG-5', target HIV-1 without disrupting endosomal sorting...
  28. pmc Cell-type-dependent targeting of human immunodeficiency virus type 1 assembly to the plasma membrane and the multivesicular body
    Akira Ono
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Virol 78:1552-63. 2004
    ..These data are consistent with a model for Gag targeting that postulates two different cellular binding partners for Gag, one on the plasma membrane and the other in the MVB...
  29. pmc Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain function
    Dimiter G Demirov
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
    Proc Natl Acad Sci U S A 99:955-60. 2002
    ..These data demonstrate a link between the E2-like domain of TSG101 and HIV-1 L domain function, and indicate that TSG101 derivatives can act as potent and specific inhibitors of HIV-1 replication by blocking virus budding...
  30. pmc Dominant negative inhibition of human immunodeficiency virus particle production by the nonmyristoylated form of gag
    Shigeo Kawada
    Kitasato Institute for Life Sciences and Graduate School for Infection Control, Kitasato University, Shirokane 5 9 1, Minato ku, Tokyo 108 8641, Japan
    J Virol 82:4384-99. 2008
    ..Overall, our data indicate that coassembly with the nonmyristoylated Gag impairs HIV particle release, a phenomenon that may involve NC-mediated Gag-Gag interaction...
  31. ncbi request reprint Pravastatin does not have a consistent antiviral effect in chronically HIV-infected individuals on antiretroviral therapy
    Peter A Sklar
    AIDS 19:1109-11. 2005
  32. ncbi request reprint Sliding movements of molluscan and algal myosin attached to a magnetizable bead under a load controlled by electromagnet
    Takashi Watari
    Department of Biology, Faculty of Science, Kobe University, Kobe, 657 8501 Japan
    J Physiol Sci 56:13-20. 2006
    ....

Research Grants5

  1. Mechanisms that determine subcellular sites of HIV-1 assembly
    Akira Ono; Fiscal Year: 2007
    ..The experiments proposed here will determine the roles played by PI(4,5)P2 in HIV-1 particle production and will contribute to the future development of antiviral drugs that target HIV-1 assembly and release. ..
  2. Mechanisms that determine subcellular sites of HIV-1 assembly
    Akira Ono; Fiscal Year: 2009
    ..The experiments proposed here will determine the roles played by PI(4,5)P2 in HIV-1 particle production and will contribute to the future development of antiviral drugs that target HIV-1 assembly and release. ..
  3. Mechanisms that determine subcellular sites of HIV-1 assembly
    Akira Ono; Fiscal Year: 2010
    ..The experiments proposed here will determine the roles played by PI(4,5)P2 in HIV-1 particle production and will contribute to the future development of antiviral drugs that target HIV-1 assembly and release. ..
  4. Mechanisms that determine subcellular sites of HIV-1 assembly
    Akira Ono; Fiscal Year: 2010
    ..The experiments proposed here will determine the roles played by PI(4,5)P2 in HIV-1 particle production and will contribute to the future development of antiviral drugs that target HIV-1 assembly and release. ..