Affiliation: University of Missouri-Kansas City
O Connor M, Gregory S. Inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function in wild-type Escherichia coli and Salmonella enterica. J Bacteriol. 2011;193:154-62 pubmed publisher
..coli K-12 strains carrying a partially defective RF2 protein are especially susceptible to perturbation of ribosome-RF interactions, such as that caused by loss of h69 ? modifications. ..
Agarwal D, Kamath D, Gregory S, O Connor M. Modulation of decoding fidelity by ribosomal proteins S4 and S5. J Bacteriol. 2015;197:1017-25 pubmed publisher
..A substantial number of the mutations alter distant locations and conceivably affect tRNA selection through indirect effects on the S4-S5 interface or by altering interactions with adjacent ribosomal proteins and 16S rRNA. ..
Kamath D, Gregory S, O Connor M. The Loop 2 Region of Ribosomal Protein uS5 Influences Spectinomycin Sensitivity, Translational Fidelity, and Ribosome Biogenesis. Antimicrob Agents Chemother. 2017;61: pubmed publisher
..coli and generated 21 unique mutants. A majority of the loop 2 alterations confer resistance to spectinomycin and affect the fidelity of translation. However, only a minority show altered rRNA processing or ribosome biogenesis defects. ..
O Connor M, Gregory S, Dahlberg A. Multiple defects in translation associated with altered ribosomal protein L4. Nucleic Acids Res. 2004;32:5750-6 pubmed
..These results suggest that mutations in the large subunit protein L4 not only alter the structure of the 50S subunit, but upon subunit association, also affect the structure and function of the 30S subunit. ..
O Connor M. Minimal translation of the tmRNA tag-coding region is required for ribosome release. Biochem Biophys Res Commun. 2007;357:276-81 pubmed
..These results suggest that the main function of tmRNA in releasing stalled ribosomes is to supply a stop codon and so facilitate termination and subsequent ribosome recycling. ..
O Connor M. Interactions of release factor RF3 with the translation machinery. Mol Genet Genomics. 2015;290:1335-44 pubmed publisher
VanNice J, Gregory S, Kamath D, O Connor M. Alterations in ribosomal protein L19 that decrease the fidelity of translation. Biochimie. 2016;128-129:122-6 pubmed publisher
..Examination of the E.Â coli 70S ribosome structure indicates that the amino acid substitutions cluster in three distinct regions of L19 and thereby potentially affect its interactions with L14 and 16S rRNA. ..
Kamath D, Allgeyer B, Gregory S, Bielski M, Roelofsz D, Sabapathypillai S, et al
. The C-terminus of ribosomal protein uS4 contributes to small ribosomal subunit biogenesis and the fidelity of translation. Biochimie. 2017;138:194-201 pubmed publisher
..coli mutants and their likely structural effects on uS4-uS5 interactions. ..
O Connor M, Leppik M, Remme J. Pseudouridine-Free Escherichia coli Ribosomes. J Bacteriol. 2018;200: pubmed publisher
..In contrast to the equivalent eukaryotic mutants, the E. coli strain is only modestly affected in growth, decoding, and ribosome biogenesis, indicating a differential requirement for Ψ modifications in these two domains. ..