DAVID H O'CONNOR

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Low-cost ultra-wide genotyping using Roche/454 pyrosequencing for surveillance of HIV drug resistance
    Dawn M Dudley
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 7:e36494. 2012
  2. pmc Discovery and full genome characterization of two highly divergent simian immunodeficiency viruses infecting black-and-white colobus monkeys (Colobus guereza) in Kibale National Park, Uganda
    Michael Lauck
    Wisconsin National Primate Research Center, Madison, WI, USA
    Retrovirology 10:107. 2013
  3. pmc T cell response specificity and magnitude against SIVmac239 are not concordant in major histocompatibility complex-matched animals
    Brian T Cain
    Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin, USA
    Retrovirology 10:116. 2013
  4. ncbi request reprint Cytotoxic T-lymphocyte escape monitoring in simian immunodeficiency virus vaccine challenge studies
    DAVID H O'CONNOR
    University of Wisconsin at Madison, Department of Pathology, Madison, Wisconsin 53709, USA
    DNA Cell Biol 21:659-64. 2002
  5. pmc Immune responses and HIV: a little order from the chaos
    DAVID H O'CONNOR
    University of Wisconsin Madison, Department of Pathology and Laboratory Medicine, Madison, WI 53711, USA
    J Exp Med 203:501-3. 2006
  6. pmc Conditional CD8+ T cell escape during acute simian immunodeficiency virus infection
    Shelby L O'Connor
    Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin, USA
    J Virol 86:605-9. 2012
  7. pmc Consequences of cytotoxic T-lymphocyte escape: common escape mutations in simian immunodeficiency virus are poorly recognized in naive hosts
    Thomas C Friedrich
    Wisconsin National Primate Research Center, 1220 Capitol Court, Madison, WI 53715, USA
    J Virol 78:10064-73. 2004
  8. pmc Mauritian cynomolgus macaques share two exceptionally common major histocompatibility complex class I alleles that restrict simian immunodeficiency virus-specific CD8+ T cells
    Benjamin J Burwitz
    Department of Pathology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 83:6011-9. 2009
  9. pmc Cytotoxic T-lymphocyte escape does not always explain the transient control of simian immunodeficiency virus SIVmac239 viremia in adenovirus-boosted and DNA-primed Mamu-A*01-positive rhesus macaques
    Adrian B McDermott
    Wisconsin National Primate Center, 1220 Capitol Court, Madison, WI 53715, USA
    J Virol 79:15556-66. 2005
  10. pmc Tat-vaccinated macaques do not control simian immunodeficiency virus SIVmac239 replication
    Todd M Allen
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 53715, USA
    J Virol 76:4108-12. 2002

Detail Information

Publications67

  1. pmc Low-cost ultra-wide genotyping using Roche/454 pyrosequencing for surveillance of HIV drug resistance
    Dawn M Dudley
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 7:e36494. 2012
    ..However, traditional HIV genotyping is extremely expensive, providing a cost barrier to wide-scale and frequent HIV drug resistance surveillance...
  2. pmc Discovery and full genome characterization of two highly divergent simian immunodeficiency viruses infecting black-and-white colobus monkeys (Colobus guereza) in Kibale National Park, Uganda
    Michael Lauck
    Wisconsin National Primate Research Center, Madison, WI, USA
    Retrovirology 10:107. 2013
    ....
  3. pmc T cell response specificity and magnitude against SIVmac239 are not concordant in major histocompatibility complex-matched animals
    Brian T Cain
    Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin, USA
    Retrovirology 10:116. 2013
    ..Here we used a genetically restricted population of Mauritian cynomolgus macaques (MCM) to characterize T cell responses within nine pairs of MHC-matched animals...
  4. ncbi request reprint Cytotoxic T-lymphocyte escape monitoring in simian immunodeficiency virus vaccine challenge studies
    DAVID H O'CONNOR
    University of Wisconsin at Madison, Department of Pathology, Madison, Wisconsin 53709, USA
    DNA Cell Biol 21:659-64. 2002
    ..To illustrate the importance of escape analysis, we show that rapid emergence of escape variants postchallenge contributed to the failure of a DNA prime/MVA boost vaccine regimen encoding SIV Tat...
  5. pmc Immune responses and HIV: a little order from the chaos
    DAVID H O'CONNOR
    University of Wisconsin Madison, Department of Pathology and Laboratory Medicine, Madison, WI 53711, USA
    J Exp Med 203:501-3. 2006
    ..We can't expect to predict details of the struggle between virus and immunity, right? Wrong--maybe we can make some predictions, say two new landmark studies with potentially huge consequences for AIDS vaccine design...
  6. pmc Conditional CD8+ T cell escape during acute simian immunodeficiency virus infection
    Shelby L O'Connor
    Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin, USA
    J Virol 86:605-9. 2012
    ..This demonstrates that the entire T cell repertoire, rather than a single T cell population, influences the timing of immune escape, thereby providing the first example of conditional CD8+ T cell escape in HIV/SIV infection...
  7. pmc Consequences of cytotoxic T-lymphocyte escape: common escape mutations in simian immunodeficiency virus are poorly recognized in naive hosts
    Thomas C Friedrich
    Wisconsin National Primate Research Center, 1220 Capitol Court, Madison, WI 53715, USA
    J Virol 78:10064-73. 2004
    ..Moreover, since the mutant epitope sequences did not revert to wild type during the study period, it is possible that low-frequency CTL exerted enough selective pressure to preserve epitope mutations in viruses replicating in vivo...
  8. pmc Mauritian cynomolgus macaques share two exceptionally common major histocompatibility complex class I alleles that restrict simian immunodeficiency virus-specific CD8+ T cells
    Benjamin J Burwitz
    Department of Pathology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 83:6011-9. 2009
    ....
  9. pmc Cytotoxic T-lymphocyte escape does not always explain the transient control of simian immunodeficiency virus SIVmac239 viremia in adenovirus-boosted and DNA-primed Mamu-A*01-positive rhesus macaques
    Adrian B McDermott
    Wisconsin National Primate Center, 1220 Capitol Court, Madison, WI 53715, USA
    J Virol 79:15556-66. 2005
    ..These results suggest that viral "breakthrough" in vaccinated animals and viral escape are not inextricably linked and underscore the need for additional research into the mechanisms of vaccine failure...
  10. pmc Tat-vaccinated macaques do not control simian immunodeficiency virus SIVmac239 replication
    Todd M Allen
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 53715, USA
    J Virol 76:4108-12. 2002
    ..Despite the induction of Tat-specific CTL, there was no significant reduction in either peak or viral set point compared to that of controls...
  11. pmc Whole-genome characterization of human and simian immunodeficiency virus intrahost diversity by ultradeep pyrosequencing
    Benjamin N Bimber
    University of Wisconsin Madison, Madison, WI 53711, USA
    J Virol 84:12087-92. 2010
    ..While this work applies pyrosequencing to immunodeficiency viruses, this approach could be applied to virtually any viral pathogen...
  12. pmc Identification of novel MHC class I sequences in pig-tailed macaques by amplicon pyrosequencing and full-length cDNA cloning and sequencing
    Claire E O'Leary
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53711, USA
    Immunogenetics 61:689-701. 2009
    ....
  13. pmc Specific CD8+ T cell responses correlate with control of simian immunodeficiency virus replication in Mauritian cynomolgus macaques
    Melisa L Budde
    Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, USA
    J Virol 86:7596-604. 2012
    ....
  14. pmc Characterization of 47 MHC class I sequences in Filipino cynomolgus macaques
    Kevin J Campbell
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53706, USA
    Immunogenetics 61:177-87. 2009
    ..These findings have implications for the evolutionary history of Filipino cynomolgus macaques as well as for the use of this model in SIV/SHIV research protocols...
  15. ncbi request reprint Reversion of CTL escape-variant immunodeficiency viruses in vivo
    Thomas C Friedrich
    Wisconsin National Primate Research Center, Madison, Wisconsin 53715, USA
    Nat Med 10:275-81. 2004
    ..In the absence of selective pressure upon transmission to new hosts, these original escape mutations can be lost. This suggests that some HIV CTL epitopes will be maintained in human populations...
  16. ncbi request reprint Differences between T cell epitopes recognized after immunization and after infection
    Thorsten U Vogel
    Wisconsin National Primate Research Center, University of Wisconsin, 1220 Capital Court, Madison, WI 53715, USA
    J Immunol 169:4511-21. 2002
    ..The repertoire of the immune response detected in the peripheral blood after immunization substantially differed from the immune response detected in the peripheral blood after infection...
  17. pmc Adoptive transfer of lymphocytes isolated from simian immunodeficiency virus SIVmac239Δnef-vaccinated macaques does not affect acute-phase viral loads but may reduce chronic-phase viral loads in major histocompatibility complex-matched recipients
    Justin M Greene
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Virol 87:7382-92. 2013
    ..These results may suggest that adoptive transfers influenced the course of infection despite the lack of significant differences in the viral loads among animals that received cells from vaccinated and mock-vaccinated donor animals...
  18. pmc The high-frequency major histocompatibility complex class I allele Mamu-B*17 is associated with control of simian immunodeficiency virus SIVmac239 replication
    Levi J Yant
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53711, USA
    J Virol 80:5074-7. 2006
    ..001). Mamu-B(*)17-positive macaques could, therefore, facilitate our understanding of the correlates of viral control...
  19. pmc Simian immunodeficiency virus SIVmac239 infection of major histocompatibility complex-identical cynomolgus macaques from Mauritius
    Roger W Wiseman
    Wisconsin National Primate Research Center, University of Wisconsin Madison, 555 Science Drive, Madison, WI 53706, USA
    J Virol 81:349-61. 2007
    ..Our identification of relatively abundant SIV-susceptible, MHC-identical macaques will facilitate research into protective cellular immunity...
  20. ncbi request reprint Acute phase cytotoxic T lymphocyte escape is a hallmark of simian immunodeficiency virus infection
    DAVID H O'CONNOR
    Wisconsin Regional Primate Research Center and Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin, USA
    Nat Med 8:493-9. 2002
    ..Our results suggest that acute viral escape from CTLs is a hallmark of SIV infection and that CTLs with high functional avidity can rapidly select for escape variants...
  21. pmc Ex vivo SIV-specific CD8 T cell responses in heterozygous animals are primarily directed against peptides presented by a single MHC haplotype
    Justin M Greene
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 7:e43690. 2012
    ....
  22. pmc Escape in one of two cytotoxic T-lymphocyte epitopes bound by a high-frequency major histocompatibility complex class I molecule, Mamu-A*02: a paradigm for virus evolution and persistence?
    Thorsten U Vogel
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 53715, USA
    J Virol 76:11623-36. 2002
    ..Differential selection by CTL may therefore be a paradigm of immunodeficiency virus infection...
  23. ncbi request reprint The high frequency Indian rhesus macaque MHC class I molecule, Mamu-B*01, does not appear to be involved in CD8+ T lymphocyte responses to SIVmac239
    John T Loffredo
    Wisconsin National Primate Research Center WNPRC, University of Wisconsin, Madison, WI 53715, USA
    J Immunol 175:5986-97. 2005
    ..Our results suggest that the high frequency MHC class I molecule, Mamu-B*01, is not involved in SIV-specific CD8+ T lymphocyte responses...
  24. pmc Extralymphoid CD8+ T cells resident in tissue from simian immunodeficiency virus SIVmac239{Delta}nef-vaccinated macaques suppress SIVmac239 replication ex vivo
    Justin M Greene
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, 585 Science Drive, Madison, WI 53711, USA
    J Virol 84:3362-72. 2010
    ..Moreover, these results underscore the limitations of relying on immunological measurements from peripheral blood lymphocytes in studies of protective immunity to HIV/SIV...
  25. pmc Exceptional simian hemorrhagic fever virus diversity in a wild African primate community
    Michael Lauck
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Virol 87:688-91. 2013
    ..All known SHFV variants are monophyletic and share three open reading frames not present in other arteriviruses. Our data suggest a need to modify the current arterivirus classification...
  26. pmc Extraepitopic compensatory substitutions partially restore fitness to simian immunodeficiency virus variants that escape from an immunodominant cytotoxic-T-lymphocyte response
    Thomas C Friedrich
    Wisconsin National Primate Research Center, Madison, Wisconsin 53715, USA
    J Virol 78:2581-5. 2004
    ..The extraepitopic mutations partially restore in vitro replicative fitness of viruses bearing the escape mutations. Constraints on epitope sequences may therefore play a role in determining the rate of escape from CTL responses in vivo...
  27. pmc A novel hepacivirus with an unusually long and intrinsically disordered NS5A protein in a wild Old World primate
    Michael Lauck
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Virol 87:8971-81. 2013
    ..These findings shed new light on the natural history and evolution of the hepaciviruses and on the extent of structural variation within the Flaviviridae. ..
  28. pmc Escape from CD8(+) T cell responses in Mamu-B*00801(+) macaques differentiates progressors from elite controllers
    Philip A Mudd
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, WI 53711, USA
    J Immunol 188:3364-70. 2012
    ..By contrast, virus in elite controller macaques showed little evidence of variation in epitopes recognized by immunodominant CD8(+) T lymphocytes, implying that these cells play a role in viral control...
  29. pmc GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo
    Lara Vojnov
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 6:e23515. 2011
    ..It is possible that these seven animals failed to control viral replication because of the narrow TCR repertoire expressed by the GagCM9-specific CD8(+) T cell population elicited by vaccination and infection...
  30. pmc MHC heterozygote advantage in simian immunodeficiency virus-infected Mauritian cynomolgus macaques
    Shelby L O'Connor
    Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53706, USA
    Sci Transl Med 2:22ra18. 2010
    ....
  31. pmc Acute phase CD8+ T lymphocytes against alternate reading frame epitopes select for rapid viral escape during SIV infection
    Andrew D Walsh
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 8:e61383. 2013
    ..Our data show that high frequency CTL target cryptic epitopes and exert selective pressure on SIV during the acute phase, underscoring the importance of these unique immune responses...
  32. pmc Characterization of full-length MHC class II sequences in Indonesian and Vietnamese cynomolgus macaques
    Hannah M Creager
    Wisconsin National Primate Research Center, University of Wisconsin Madison, USA
    Immunogenetics 63:611-8. 2011
    ....
  33. pmc MHC class I characterization of Indonesian cynomolgus macaques
    Chad J Pendley
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53706, USA
    Immunogenetics 60:339-51. 2008
    ....
  34. pmc Major histocompatibility complex class I alleles associated with slow simian immunodeficiency virus disease progression bind epitopes recognized by dominant acute-phase cytotoxic-T-lymphocyte responses
    DAVID H O'CONNOR
    Wisconsin Regional Primate Research Center and Department of Pathology and Laboratory Medicine, Madison, Wisconsin, USA
    J Virol 77:9029-40. 2003
    ....
  35. pmc Differential MHC class I expression in distinct leukocyte subsets
    Justin M Greene
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, 53706 Wisconsin, USA
    BMC Immunol 12:39. 2011
    ..It is unclear how cDNA species are expressed in distinct cell subsets in humans and particularly in macaques which transcribe upwards of 20 distinct MHC class I alleles at variable levels...
  36. pmc Infection with "escaped" virus variants impairs control of simian immunodeficiency virus SIVmac239 replication in Mamu-B*08-positive macaques
    Laura E Valentine
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 83:11514-27. 2009
    ..04). Our results suggest that these epitope-specific CD8+ T-cell responses may play a role in establishing the control of viral replication in Mamu-B*08+ macaques...
  37. pmc The role of MHC class I allele Mamu-A*07 during SIV(mac)239 infection
    Jason S Reed
    Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, USA
    Immunogenetics 63:789-807. 2011
    ..Thus, it appears that Mamu-A1*007:01 presents SIV-derived peptides to antigen-specific CD8(+) T cells and is part of the immune response to SIV(mac)239...
  38. pmc Transcriptionally abundant major histocompatibility complex class I alleles are fundamental to nonhuman primate simian immunodeficiency virus-specific CD8+ T cell responses
    Melisa L Budde
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 85:3250-61. 2011
    ..Thus, only a subset of the thousands of known MHC-I alleles in macaques should be prioritized for CD8(+) T cell epitope characterization...
  39. pmc Allogeneic lymphocytes persist and traffic in feral MHC-matched mauritian cynomolgus macaques
    Justin M Greene
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 3:e2384. 2008
    ..Previous adoptive transfers in primates have failed as transferred cells are typically cleared within hours after transfer...
  40. pmc Novel, divergent simian hemorrhagic fever viruses in a wild Ugandan red colobus monkey discovered using direct pyrosequencing
    Michael Lauck
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 6:e19056. 2011
    ..Here, we describe the discovery and genetic characterization by direct pyrosequencing of two novel, divergent SHFV variants co-infecting a single male red colobus monkey from Kibale National Park, Uganda...
  41. pmc Discovery and characterization of distinct simian pegiviruses in three wild African Old World monkey species
    Samuel D Sibley
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 9:e98569. 2014
    ..Our study describes the first primate pegiviruses detected in Old World monkeys, expanding the known genetic diversity and host range of pegiviruses and providing insight into the natural history of this genus. ..
  42. pmc Full-length novel MHC class I allele discovery by next-generation sequencing: two platforms are better than one
    Dawn M Dudley
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, WI, 53705, USA
    Immunogenetics 66:15-24. 2014
    ..We anticipate that this method will facilitate rapid expansion and near completion of sequence libraries of polymorphic loci, such as MHC class I, within a few years...
  43. ncbi request reprint Unusually high frequency MHC class I alleles in Mauritian origin cynomolgus macaques
    Kendall C Krebs
    Wisconsin National Primate Research Center, Madison, WI 53706, USA
    J Immunol 175:5230-9. 2005
    ....
  44. pmc Co-infection and cross-species transmission of divergent Hepatocystis lineages in a wild African primate community
    Mary I Thurber
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, WI 53706, USA
    Int J Parasitol 43:613-9. 2013
    ..Hepatocystis parasites in this primate community are a diverse assemblage of cryptic lineages, some of which co-infect hosts and at least one of which can cross primate species barriers...
  45. pmc A dominant role for CD8+-T-lymphocyte selection in simian immunodeficiency virus sequence variation
    DAVID H O'CONNOR
    Wisconsin Primate Research Center, Department of Pathology, Laboratoty of Medicine, University of Wisconsin, 1300 University Ave, Madison, WI 53706, USA
    J Virol 78:14012-22. 2004
    ..We also found that >60% of viral variation outside of the viral envelope occurs within recognized CD8-TL epitopes. Therefore, we conclude that CD8-TL selection is the dominant cause of SIV diversification outside of the envelope...
  46. pmc Nef gene evolution from a single transmitted strain in acute SIV infection
    Benjamin N Bimber
    Department of Pathology and Laboratory Medicine, Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Retrovirology 6:57. 2009
    ....
  47. pmc Rapid high-resolution MHC class I genotyping of Chinese rhesus macaques by capillary reference strand-mediated conformational analysis
    Alex J Blasky
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI, 53706, USA
    Immunogenetics 60:575-84. 2008
    ..Capillary RSCA genotyping has the potential to enable researchers to rapidly evaluate MHC class I genotypes in rhesus macaques and associate specific MHC sequences with disease susceptibility...
  48. pmc Major histocompatibility complex class I haplotype diversity in Chinese rhesus macaques
    Julie A Karl
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, Wisconsin 53715, USA
    G3 (Bethesda) 3:1195-201. 2013
    ..It may also be possible to extend these studies across multiple species of macaques, as we found evidence of shared ancestral haplotypes between Chinese rhesus and Mauritian cynomolgus macaques. ..
  49. ncbi request reprint Discovery and full genome characterization of a new SIV lineage infecting red-tailed guenons (Cercopithecus ascanius schmidti) in Kibale National Park, Uganda
    Michael Lauck
    Wisconsin National Primate Research Center, 555 Science Dr, 53705 Madison, WI, USA
    Retrovirology 11:55. 2014
    ..In this study, we screened blood specimens from red-tailed guenons (Cercopithecus ascanius schmidti) from Kibale National Park, Uganda, for the presence of novel SIVs using unbiased deep-sequencing...
  50. pmc Comprehensive characterization of MHC class II haplotypes in Mauritian cynomolgus macaques
    Shelby L O'Connor
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, WI 53706, USA
    Immunogenetics 59:449-62. 2007
    ....
  51. pmc Ultra-high resolution HLA genotyping and allele discovery by highly multiplexed cDNA amplicon pyrosequencing
    Simon M Lank
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI, USA
    BMC Genomics 13:378. 2012
    ..Achieving high-resolution typing is also becoming more challenging with traditional methods as the database of known HLA alleles increases...
  52. pmc Cost-effective sequence-based nonhuman primate MHC class I genotyping from RNA
    Julie A Karl
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53706, USA
    Methods 49:11-7. 2009
    ..In addition, full-length cDNA amplicons with novel sequences can be resequenced in their entireties to expand the repertoire of characterized MHC class I sequences for NHPs...
  53. pmc Identification of MHC class I sequences in Chinese-origin rhesus macaques
    Julie A Karl
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, WI 53706, USA
    Immunogenetics 60:37-46. 2008
    ..The discovery of 27 novel MHC class I sequences in this analysis underscores the genetic diversity of Chinese rhesus macaques and contributes reagents that will be valuable for studying cellular immunology in this population...
  54. pmc Integrin alpha4beta7 is downregulated on the surfaces of simian immunodeficiency virus SIVmac239-infected cells
    Melisa L Budde
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 84:6344-51. 2010
    ..Downregulation of integrin alpha4beta7 may have an unappreciated role in the CD4 depletion of the mucosal-associated lymphoid compartments, susceptibility to superinfection, and/or immune evasion...
  55. ncbi request reprint KIRigami: the case for studying NK cell receptors in SIV+ macaques
    Benjamin Bimber
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, WI 53706, USA
    Immunol Res 40:235-43. 2008
    ..This review focuses on the current understanding of the role of KIRs in HIV infection and outstanding questions for future study...
  56. pmc Pyrosequencing reveals restricted patterns of CD8+ T cell escape-associated compensatory mutations in simian immunodeficiency virus
    Benjamin J Burwitz
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin 537061, USA
    J Virol 85:13088-96. 2011
    ..This is the first detailed report of the ontogeny of compensatory mutations that allow CD8+ T cell epitope escape in infected individuals...
  57. pmc Characterization of killer immunoglobulin-like receptor genetics and comprehensive genotyping by pyrosequencing in rhesus macaques
    Anna J Moreland
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    BMC Genomics 12:295. 2011
    ..Rhesus macaques serve as important animal models for many human diseases in which KIRs are implicated; however, the study of KIR activity in this model is hindered by incomplete characterization of KIR genetics...
  58. pmc Simian immunodeficiency virus SIVmac239Deltanef vaccination elicits different Tat28-35SL8-specific CD8+ T-cell clonotypes compared to a DNA prime/adenovirus type 5 boost regimen in rhesus macaques
    Benjamin J Burwitz
    Department of Pathology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Virol 85:3683-9. 2011
    ....
  59. pmc Complete characterization of killer Ig-like receptor (KIR) haplotypes in Mauritian cynomolgus macaques: novel insights into nonhuman primate KIR gene content and organization
    Benjamin N Bimber
    Department of Pathology and Laboratory Medicine, Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53706, USA
    J Immunol 181:6301-8. 2008
    ..The findings from this study fundamentally advance our understanding of KIR genetics in nonhuman primates and establish a foundation from which to study KIR signaling in disease pathogenesis...
  60. pmc Major histocompatibility complex genotyping with massively parallel pyrosequencing
    Roger W Wiseman
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, Wisconsin, USA
    Nat Med 15:1322-6. 2009
    ..The remarkable sensitivity of this approach in macaques demonstrates that pyrosequencing is viable for ultra-high-throughput MHC genotyping of primates, including humans...
  61. pmc Vaccine-induced cellular immune responses reduce plasma viral concentrations after repeated low-dose challenge with pathogenic simian immunodeficiency virus SIVmac239
    Nancy A Wilson
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, WI 53711, USA
    J Virol 80:5875-85. 2006
    ....
  62. ncbi request reprint Pirate primates in uncharted waters: lymphocyte transfers in unrelated, MHC-matched macaques
    Benjamin J Burwitz
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, USA
    Curr HIV Res 7:51-6. 2009
    ..Here we review the current state of SIV adoptive transfer research and present a novel macaque model that allows for allogeneic adoptive transfers...
  63. pmc Analysis of hepatitis C virus intrahost diversity across the coding region by ultradeep pyrosequencing
    Michael Lauck
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Virol 86:3952-60. 2012
    ..The approach described here is broadly applicable to studies of viral diversity and could help to improve the efficacy of direct-acting antiviral agents (DAA) in the treatment of HCV-infected patients...
  64. pmc A novel single cDNA amplicon pyrosequencing method for high-throughput, cost-effective sequence-based HLA class I genotyping
    Simon M Lank
    Wisconsin National Primate Research Center, University of Wisconsin Madison, Madison, Wisconsin, USA
    Hum Immunol 71:1011-7. 2010
    ..Unprecedented typing throughput results from a novel single cDNA-PCR amplicon strategy requiring only 1 PCR amplification per sample. This method dramatically reduces cost for genotyping of large cohorts...
  65. pmc Effects of cytotoxic T lymphocytes (CTL) directed against a single simian immunodeficiency virus (SIV) Gag CTL epitope on the course of SIVmac239 infection
    Todd M Allen
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
    J Virol 76:10507-11. 2002
    ..By themselves, these strong CTL responses failed to control SIVmac239 replication...
  66. pmc Hepatitis C virus NS3/NS4A DNA vaccine induces multiepitope T cell responses in rhesus macaques mimicking human immune responses [corrected]
    Krystle A Lang Kuhs
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Ther 20:669-78. 2012
    ..Taken together our findings suggest that the rhesus macaque vaccination model is a useful tool in the evaluation of immune responses induced by HCV immunogens...
  67. pmc Characterization of Mauritian cynomolgus macaque major histocompatibility complex class I haplotypes by high-resolution pyrosequencing
    Melisa L Budde
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, WI 53705, USA
    Immunogenetics 62:773-80. 2010
    ..Overall, this approach significantly expanded our knowledge of MCM haplotypes and will facilitate future studies on disease pathogenesis and protective cellular immunity...

Research Grants7

  1. SIV-Specific CD8+ T-Cell in Mauritian Cynomolgus Macaques
    DAVID OCONNOR; Fiscal Year: 2007
    ..These studies exploit the unique genetics of Mauritian Cynomolgus macaques to explore fundamental questions about the protective value of CD8+ T-lymphocyte responses in SIV infection. ..
  2. Immunogenetics of Primates Used for Bioterror Research
    DAVID OCONNOR; Fiscal Year: 2007
    ..abstract_text> ..
  3. Adoptive transfer of immunity elicited by attenuated HIV vaccines
    DAVID OCONNOR; Fiscal Year: 2009
    ..For the first time, we will attempt to transfer this immunity between animals in order to understand which arms of the immune response are involved in protection. ..
  4. Adoptive transfer of immunity elicited by attenuated HIV vaccines
    DAVID H OCONNOR; Fiscal Year: 2010
    ..For the first time, we will attempt to transfer this immunity between animals in order to understand which arms of the immune response are involved in protection. ..