MARK R O'BRIAN

Summary

Affiliation: University at Buffalo
Country: USA

Publications

  1. pmc Bradyrhizobium japonicum senses iron through the status of haem to regulate iron homeostasis and metabolism
    Jianhua Yang
    Department of Biochemistry, 140 Farber Hall, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Mol Microbiol 60:427-37. 2006
  2. pmc Identification of a soybean protein that interacts with GAGA element dinucleotide repeat DNA
    Indu Sangwan
    Department of Biochemistry, State University of New York, Buffalo, New York 14214, USA
    Plant Physiol 129:1788-94. 2002
  3. pmc Positive control of ferric siderophore receptor gene expression by the Irr protein in Bradyrhizobium japonicum
    Sandra K Small
    Department of Biochemistry, State University of New York at Buffalo, 14214, USA
    J Bacteriol 191:1361-8. 2009
  4. ncbi request reprint A novel DNA-binding site for the ferric uptake regulator (Fur) protein from Bradyrhizobium japonicum
    Yali E Friedman
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Biol Chem 278:38395-401. 2003
  5. pmc Transcriptional control of the Bradyrhizobium japonicum irr gene requires repression by fur and Antirepression by Irr
    Thomas H Hohle
    Department of Biochemistry, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Biol Chem 285:26074-80. 2010
  6. pmc Bacterial outer membrane channel for divalent metal ion acquisition
    Thomas H Hohle
    Department of Biochemistry, State University of New York, Buffalo, NY 14214, USA
    Proc Natl Acad Sci U S A 108:15390-5. 2011
  7. ncbi request reprint Oxidative stress promotes degradation of the Irr protein to regulate haem biosynthesis in Bradyrhizobium japonicum
    Jianhua Yang
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA
    Mol Microbiol 60:209-18. 2006
  8. ncbi request reprint The ferric uptake regulator (Fur) protein from Bradyrhizobium japonicum is an iron-responsive transcriptional repressor in vitro
    Yali E Friedman
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York, Buffalo, 14214, USA
    J Biol Chem 279:32100-5. 2004
  9. pmc Heme-dependent metalloregulation by the iron response regulator (Irr) protein in Rhizobium and other Alpha-proteobacteria
    Sandra K Small
    Department of Biochemistry and the Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Biometals 22:89-97. 2009
  10. pmc The mntH gene encodes the major Mn(2+) transporter in Bradyrhizobium japonicum and is regulated by manganese via the Fur protein
    Thomas H Hohle
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA
    Mol Microbiol 72:399-409. 2009

Research Grants

  1. Regulation of Bacterial Heme Biosynthesis
    MARK O BRIAN; Fiscal Year: 2004
  2. 2004 Tetrapyrroles Gordon Conference
    MARK O BRIAN; Fiscal Year: 2004
  3. Regulation of Bacterial Heme Biosynthesis
    MARK O BRIAN; Fiscal Year: 2005
  4. Regulation of Bacterial Heme Biosynthesis
    MARK O BRIAN; Fiscal Year: 2006
  5. Regulation of Bacterial Heme Biosynthesis
    MARK O BRIAN; Fiscal Year: 2007
  6. Regulation of Bacterial Heme Metabolism
    MARK O BRIAN; Fiscal Year: 2009
  7. Regulation of Bacterial Heme Metabolism
    MARK R O apos BRIAN; Fiscal Year: 2010
  8. Regulation of Bacterial Heme Metabolism
    MARK R O apos BRIAN; Fiscal Year: 2011

Collaborators

  • Rosalba Escamilla-Hernandez
  • Gary Stacey
  • MARK O BRIAN
  • MARK R O apos BRIAN
  • Thomas H Hohle
  • Sandra K Small
  • Jianhua Yang
  • Indu Sangwan
  • Sumant Puri
  • Tao Gao
  • Yali E Friedman
  • Heather R Panek
  • Zhenhao Qi
  • Heather Panek
  • William L Franck
  • Hans Martin Fischer
  • Hauke Hennecke
  • Felix Hauser
  • Andrea Lindemann
  • Koichiro Ishimori

Detail Information

Publications24

  1. pmc Bradyrhizobium japonicum senses iron through the status of haem to regulate iron homeostasis and metabolism
    Jianhua Yang
    Department of Biochemistry, 140 Farber Hall, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Mol Microbiol 60:427-37. 2006
    ..In addition, the haem mutant had an abnormally high cellular iron content. The findings indicate that B. japonicum senses iron via the status of haem biosynthesis in an Irr-dependent manner to regulate iron homeostasis and metabolism...
  2. pmc Identification of a soybean protein that interacts with GAGA element dinucleotide repeat DNA
    Indu Sangwan
    Department of Biochemistry, State University of New York, Buffalo, New York 14214, USA
    Plant Physiol 129:1788-94. 2002
    ..Homologs of GBP were found in other dicots and in the monocot rice (Oryza sativa), as well. We suggest that interaction between GAGA elements and GBP-like proteins is a regulatory feature in plants...
  3. pmc Positive control of ferric siderophore receptor gene expression by the Irr protein in Bradyrhizobium japonicum
    Sandra K Small
    Department of Biochemistry, State University of New York at Buffalo, 14214, USA
    J Bacteriol 191:1361-8. 2009
    ..Chromatin immunoprecipitation experiments demonstrated that Irr occupies the promoters of the five ferric iron transport genes in vivo. We conclude that Irr is a direct positive regulator of ferric iron transport in B. japonicum...
  4. ncbi request reprint A novel DNA-binding site for the ferric uptake regulator (Fur) protein from Bradyrhizobium japonicum
    Yali E Friedman
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Biol Chem 278:38395-401. 2003
    ..In addition, Fur binding to a target promoter is sufficient to repress transcription in vitro...
  5. pmc Transcriptional control of the Bradyrhizobium japonicum irr gene requires repression by fur and Antirepression by Irr
    Thomas H Hohle
    Department of Biochemistry, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Biol Chem 285:26074-80. 2010
    ..We conclude that Fur mediates manganese-dependent repression of irr transcription and that Irr acts as an antirepressor under iron limitation by preventing Fur binding to the promoter...
  6. pmc Bacterial outer membrane channel for divalent metal ion acquisition
    Thomas H Hohle
    Department of Biochemistry, State University of New York, Buffalo, NY 14214, USA
    Proc Natl Acad Sci U S A 108:15390-5. 2011
    ..We suggest that the outer membrane is a barrier to divalent metal ions that requires a selective channel to meet the nutritional needs of the cell...
  7. ncbi request reprint Oxidative stress promotes degradation of the Irr protein to regulate haem biosynthesis in Bradyrhizobium japonicum
    Jianhua Yang
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA
    Mol Microbiol 60:209-18. 2006
    ..We suggest that Irr oxidation is a signal for its degradation, and that cells sense and respond to oxidative stress through Irr to regulate haem biosynthesis...
  8. ncbi request reprint The ferric uptake regulator (Fur) protein from Bradyrhizobium japonicum is an iron-responsive transcriptional repressor in vitro
    Yali E Friedman
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York, Buffalo, 14214, USA
    J Biol Chem 279:32100-5. 2004
    ..We suggest that the putative functions for metal-binding sites deduced from the structure of P. aeruginosa Fur cannot be extrapolated to bacterial Fur proteins as a whole...
  9. pmc Heme-dependent metalloregulation by the iron response regulator (Irr) protein in Rhizobium and other Alpha-proteobacteria
    Sandra K Small
    Department of Biochemistry and the Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Biometals 22:89-97. 2009
    ..Studies with Rhizobium reveal that the regulation of iron homeostasis in bacteria is more diverse than has been generally assumed...
  10. pmc The mntH gene encodes the major Mn(2+) transporter in Bradyrhizobium japonicum and is regulated by manganese via the Fur protein
    Thomas H Hohle
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, Buffalo, NY 14214, USA
    Mol Microbiol 72:399-409. 2009
    ..japonicum, and show that Fur is a manganese-responsive regulator in that organism. Furthermore, Fe(2+) is neither a substrate for MntH nor a regulator of mntH expression in vivo...
  11. pmc Control of bacterial iron homeostasis by manganese
    Sumant Puri
    Department of Biochemistry, State University of New York, Buffalo, NY 14214, USA
    Proc Natl Acad Sci U S A 107:10691-5. 2010
    ..The findings implicate a mechanism for the control of iron homeostasis by manganese in a bacterium...
  12. pmc HmuP is a coactivator of Irr-dependent expression of heme utilization genes in Bradyrhizobium japonicum
    Rosalba Escamilla-Hernandez
    Department of Biochemistry, State University of New York at Buffalo, Buffalo, New York, USA
    J Bacteriol 194:3137-43. 2012
    ..Thus, both HmuP and Irr occupancy are necessary for hmuR induction. We suggest that HmuP is a coactivator of Irr-dependent expression of hmuR...
  13. pmc The Bradyrhizobium japonicum Irr protein is a transcriptional repressor with high-affinity DNA-binding activity
    Indu Sangwan
    Department of Biochemistry, 140 Farber Hall, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Bacteriol 190:5172-7. 2008
    ..The findings demonstrate that Irr is a transcriptional repressor that binds DNA with high affinity...
  14. ncbi request reprint The Bradyrhizobium japonicum Fur protein is an iron-responsive regulator in vivo
    Jianhua Yang
    Department of Biochemistry, State University of New York at Buffalo, 140 Farber Hall, Buffalo, NY 14214, USA
    Mol Genet Genomics 276:555-64. 2006
    ..The findings show that B. japonicum Fur is involved in iron-dependent gene expression, and support the conclusion that rhizobial Fur proteins have novel functions compared with well studied model systems...
  15. ncbi request reprint Two heme binding sites are involved in the regulated degradation of the bacterial iron response regulator (Irr) protein
    Jianhua Yang
    Department of Biochemistry, State University of New York, Buffalo, New York 14214, USA
    J Biol Chem 280:7671-6. 2005
    ..We conclude that normal iron-dependent Irr degradation involves two heme binding sites and that both redox states of heme are required for rapid turnover...
  16. pmc Control of DegP-dependent degradation of c-type cytochromes by heme and the cytochrome c maturation system in Escherichia coli
    Tao Gao
    Department of Biochemistry, 140 Farber Hall, State University of New York at Buffalo, Buffalo, NY 14214, USA
    J Bacteriol 189:6253-9. 2007
    ..The data show that the cytochrome c heme-binding motif is an instability element and that stabilization by Ccm does not require ligation of the heme moiety to the protein...
  17. ncbi request reprint Interaction between the bacterial iron response regulator and ferrochelatase mediates genetic control of heme biosynthesis
    Zhenhao Qi
    Department of Biochemistry, 140 Farber Hall, The State University of New York at Buffalo, Buffalo, NY 14214, USA
    Mol Cell 9:155-62. 2002
    ..Thus, metabolic control of the heme pathway involves a regulatory function of a biosynthetic enzyme to affect gene expression. Furthermore, heme can serve as a signaling molecule without accumulating freely in cells...
  18. pmc The hmuQ and hmuD genes from Bradyrhizobium japonicum encode heme-degrading enzymes
    Sumant Puri
    Department of Biochemistry, 140 Farber Hall, State University of New York at Buffalo, Buffalo, NY 14214, USA
    J Bacteriol 188:6476-82. 2006
    ..The findings show that hmuQ and hmuD encode heme oxygenases and indicate that the IsdG family of heme-degrading monooxygenases is not restricted to gram-positive pathogenic bacteria...
  19. pmc Iron-dependent cytochrome c1 expression is mediated by the status of heme in Bradyrhizobium japonicum
    Tao Gao
    Department of Biochemistry, 140 Farber Hall, State University of New York at Buffalo, Buffalo, NY 14214, USA
    J Bacteriol 187:5084-9. 2005
    ..We suggest that iron-dependent cytochrome c1 expression is mediated by heme availability for heme protein formation...
  20. pmc Manganese is required for oxidative metabolism in unstressed Bradyrhizobium japonicum cells
    Thomas H Hohle
    Department of Biochemistry, State University of New York at Buffalo, Buffalo, NY 14214, USA
    Mol Microbiol 84:766-77. 2012
    ..The findings implicate roles for manganese in key steps in unstressed oxidative metabolism in B. japonicum...
  21. pmc The Bradyrhizobium japonicum frcB gene encodes a diheme ferric reductase
    Sandra K Small
    Department of Biochemistry, State University of New York at Buffalo, 140 Farber Hall, Buffalo, NY 14214, USA
    J Bacteriol 193:4088-94. 2011
    ..This suggests that membrane-bound, heme-containing ferric reductase proteins are not confined to eukaryotes but may be common in bacteria...
  22. ncbi request reprint A whole genome view of prokaryotic haem biosynthesis
    Heather Panek
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis, State University of New York at Buffalo, Buffalo, NY 14214, USA
    Microbiology 148:2273-82. 2002
  23. pmc KatG is the primary detoxifier of hydrogen peroxide produced by aerobic metabolism in Bradyrhizobium japonicum
    Heather R Panek
    Department of Biochemistry and Witebsky Center for Microbial Pathogenesis and Immunology, State University of New York at Buffalo, NY 14214, USA
    J Bacteriol 186:7874-80. 2004
    ..We conclude that aerobic metabolism produces toxic levels of H(2)O(2) in B. japonicum, which is detoxified primarily by KatG. Furthermore, the katG level sufficient for detoxification does not require OxyR...
  24. ncbi request reprint Biochemistry, regulation and genomics of haem biosynthesis in prokaryotes
    MARK R O'BRIAN
    Department of Biochemistry, State University of New York at Buffalo, Buffalo, NY 14214, USA
    Adv Microb Physiol 46:257-318. 2002
    ..Finally, a whole genome analysis from 63 prokaryotes indicates intriguing exceptions to the universality of the haem biosynthetic pathway and helps define new frontiers for future study...

Research Grants8

  1. Regulation of Bacterial Heme Biosynthesis
    MARK O BRIAN; Fiscal Year: 2004
    ..3. Characterize Irr regulatory function with respect to its DNA-binding properties, functional domains and gene targets. ..
  2. 2004 Tetrapyrroles Gordon Conference
    MARK O BRIAN; Fiscal Year: 2004
    ..Strengths of this Gordon conference are its disciplinary diversity, focus on new unpublished work, time for discussion, informal atmosphere and ban on dissemination of conference proceedings. ..
  3. Regulation of Bacterial Heme Biosynthesis
    MARK O BRIAN; Fiscal Year: 2005
    ..3. Characterize Irr regulatory function with respect to its DNA-binding properties, functional domains and gene targets. ..
  4. Regulation of Bacterial Heme Biosynthesis
    MARK O BRIAN; Fiscal Year: 2006
    ..3. Characterize Irr regulatory function with respect to its DNA-binding properties, functional domains and gene targets. ..
  5. Regulation of Bacterial Heme Biosynthesis
    MARK O BRIAN; Fiscal Year: 2007
    ..3. Characterize Irr regulatory function with respect to its DNA-binding properties, functional domains and gene targets. ..
  6. Regulation of Bacterial Heme Metabolism
    MARK O BRIAN; Fiscal Year: 2009
    ..We are studying novel mechanisms of bacterial heme and iron metabolism in B. japonicum towards the end of understanding molecular strategies that bacteria employ for infection and adaptation. ..
  7. Regulation of Bacterial Heme Metabolism
    MARK R O apos BRIAN; Fiscal Year: 2010
    ..We are studying novel mechanisms of bacterial heme and iron metabolism in B. japonicum towards the end of understanding molecular strategies that bacteria employ for infection and adaptation. ..
  8. Regulation of Bacterial Heme Metabolism
    MARK R O apos BRIAN; Fiscal Year: 2011
    ..We are studying novel mechanisms of bacterial heme and iron metabolism in B. japonicum towards the end of understanding molecular strategies that bacteria employ for infection and adaptation. ..