Research Topics
Genomes and Genes | Giuseppina NuciforaSummaryAffiliation: University of Illinois at Chicago Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Association of 3q21q26 syndrome with different RPN1/EVI1 fusion transcriptsGiovanni Martinelli
Institute of Hematology and Medical Oncology L and A Seragnoli, University of Bologna, Italy
Haematologica 88:1221-8. 2003..Other genes that have been implicated at the rearrangement breakpoint are GR6 and RPN1 (both on 3q21). The aim of this study was to investigate the expression of the EVI1 fusion genes in AML patients with 3q21q26 syndrome...- EVI1 and hematopoietic disorders: history and perspectivesGiuseppina Nucifora
Department of Pathology, University of Illinois at Chicago, 60607, United States
Gene 368:1-11. 2006..In this review, we summarize the biochemical properties of EVI1 and the effects of EVI1 in biological models... - Corepressor CtBP1 interacts with and specifically inhibits CBP activityVitalyi Senyuk
Department of Pathology, The Cancer Center, University of Illinois at Chicago, Chicago, IL, USA
Arch Biochem Biophys 441:168-73. 2005..Based on these results, we propose that CtBP1 mediates repression by blocking histone acetylation by HAT complexes... - P/CAF and GCN5 acetylate the AML1/MDS1/EVI1 fusion oncoproteinVitalyi Senyuk
Department of Pathology, Molecular Biology Research Building, M C 737, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607, USA
Biochem Biophys Res Commun 307:980-6. 2003..AME acetylation has no effect on its interaction with the co-repressor CtBP1. Finally, we demonstrate that the co-expression of AME and either P/CAF or GCN5 abrogates the repression of an AML1-dependent reporter gene... 
EVI1 Impairs myelopoiesis by deregulation of PU.1 functionLeopoldo Laricchia-Robbio
Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA
Cancer Res 69:1633-42. 2009..After mapping the PU.1-EVI1 interaction sites, we show that an EVI1 point mutant, unable to bind PU.1, restores the activation of PU.1-regulated genes and allows a normal differentiation of BM progenitors in vitro...
Significant increase of self-renewal in hematopoietic cells after forced expression of EVI1Leopoldo Laricchia-Robbio
Department of Medicine, University of Illinois at Chicago M C 737, 909 South Wolcott Avenue, Chicago, IL 60612, USA
Blood Cells Mol Dis 40:141-7. 2008....- Combinatorial action of RUNX1 and PU.1 in the regulation of hematopoiesisYogen Saunthararajah
Department of Medicine and Cancer Center, University of Illinois at Chicago, Chicago, IL 60612, USA
Crit Rev Eukaryot Gene Expr 16:183-92. 2006..Here we describe the relationship of RUNX1 with PU.1 as a facet of the combinatorial relationships that determine hematopoietic lineage commitment... - The distal zinc finger domain of AML1/MDS1/EVI1 is an oligomerization domain involved in induction of hematopoietic differentiation defects in primary cells in vitroVitalyi Senyuk
Department of Pathology and The Cancer Center, University of Illinois at Chicago, Chicago, Illinois 60607, USA
Cancer Res 65:7603-11. 2005.... - The oncoprotein EVI1 and the DNA methyltransferase Dnmt3 co-operate in binding and de novo methylation of target DNAVitalyi Senyuk
Department of Medicine, University of Illinois at Chicago, Chicago, Illinois, United States of America
PLoS ONE 6:e20793. 2011.... - The leukemia-associated transcription repressor AML1/MDS1/EVI1 requires CtBP to induce abnormal growth and differentiation of murine hematopoietic cellsVitalyi Senyuk
Department of Pathology, The Cancer Center, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607, USA
Oncogene 21:3232-40. 2002.... - A new translocation that rearranges the AML1 gene in a patient with T-cell acute lymphoblastic leukemiaFady M Mikhail
Department of Clinical Pathology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
Cancer Genet Cytogenet 135:96-100. 2002..By metaphase fluorescence in situ hybridization analysis, the AML1 breakpoint was mapped using recombinant phage clones, and shown to be either immediately upstream or downstream of exon 5... - Repression of RUNX1 activity by EVI1: a new role of EVI1 in leukemogenesisVitalyi Senyuk
Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA
Cancer Res 67:5658-66. 2007.... - RUNX1-RUNX1 homodimerization modulates RUNX1 activity and functionDonglan Li
Department of Medicine, University of Illinois, Chicago, Illinois 60612, USA
J Biol Chem 282:13542-51. 2007.... - Point mutations in two EVI1 Zn fingers abolish EVI1-GATA1 interaction and allow erythroid differentiation of murine bone marrow cellsLeopoldo Laricchia-Robbio
University of Illinois at Chicago (M/C 737, 909 South Wolcott Avenue, Chicago, IL 60612, USA
Mol Cell Biol 26:7658-66. 2006..Point mutations that disrupt the geometry of two zinc fingers of EVI1 abolish the protein-protein interaction, leading to normal erythroid differentiation of normal murine bone marrow in vitro... - Small ubiquitin-like modifier conjugation regulates nuclear export of TEL, a putative tumor suppressorLauren D Wood
Department of Biochemistry and Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
Proc Natl Acad Sci U S A 100:3257-62. 2003..We propose that the ability of TEL to repress transcription and suppress growth is regulated by sumoylation and nuclear export... - Meeting report: Sixth International Workshop on Molecular Aspects of Myeloid Stem Cell Development and Leukemia, Annapolis, May 1-4, 2005Linda Wolff
National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Exp Hematol 33:1436-42. 2005 - SUV39H1 interacts with AML1 and abrogates AML1 transactivity. AML1 is methylated in vivoSoumen Chakraborty
Department of Pathology and The Cancer Center, University of Illinois at Chicago, Chicago, IL 60607, USA
Oncogene 22:5229-37. 2003..Our data suggest that other enzymes are also involved in gene regulation by AML1 activity by modulating the affinity of AML1 for DNA... - Normal and transforming functions of RUNX1: a perspectiveFady M Mikhail
Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA
J Cell Physiol 207:582-93. 2006..Here, we provide an overview of the many roles of RUNX1 in hematopoietic self-renewal and differentiation and summarize the information that is currently available on the many mechanisms of RUNX1 deregulation in human leukemia... - The role of EVI1 in normal and leukemic cellsSilvia Buonamici
Department of Pathology and Cancer Center, University of Illinois at Chicago, Chicago, IL 60607, USA
Blood Cells Mol Dis 31:206-12. 2003.... - EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activityYiqing Chi
Department of Pathology and The Cancer Center, University of Illinois, Chicago, Illinois 60607, USA
J Biol Chem 278:49806-11. 2003..Taken together, these data support the hypothesis that the interaction with BRG1 is important for up-regulation of cell-growth by EVI1... - EVI1 abrogates interferon-alpha response by selectively blocking PML inductionSilvia Buonamici
Department of Pathology and Cancer Center, University of Illinois, Chicago, Illinois 60607, USA
J Biol Chem 280:428-36. 2005..These results point to a novel mechanism utilized by an oncogene to escape normal cell response to growth-controlling cytokines... - EVI1 induces myelodysplastic syndrome in miceSilvia Buonamici
Department of Pathology and Cancer Center, University of Illinois at Chicago, Chicago, Illinois, USA
J Clin Invest 114:713-9. 2004..These defects are not fatal, and the mice survive for about 10 months with compensated hematopoiesis. Over this time, compensation fails, and the mice succumb to fatal peripheral cytopenia... - Arsenic trioxide and thalidomide combination produces multi-lineage hematological responses in myelodysplastic syndromes patients, particularly in those with high pre-therapy EVI1 expressionAzra Raza
Section of Myeloid Diseases and MDS Center, Rush University, Rush Presbyterian St Luke s Medical Center, 2242 West Harrison Street, Suite 108, Chicago, IL 60612 3515, USA
Leuk Res 28:791-803. 2004..Both low/high risk MDS may benefit significantly from therapy with ATO/thalidomide, and those with high pre-therapy EVI1 expression may be uniquely sensitive... - A novel gene, FGA7, is fused to RUNX1/AML1 in a t(4;21)(q28;q22) in a patient with T-cell acute lymphoblastic leukemiaFady M Mikhail
Department of Clinical Pathology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
Genes Chromosomes Cancer 39:110-8. 2004..It is possible that the expression of a constitutively shortened AML1 could compete with full-length AML1 and act as a dominant negative inhibitor of the promoters that the core binding factor activates... - Genomic DNA breakpoints in AML1/RUNX1 and ETO cluster with topoisomerase II DNA cleavage and DNase I hypersensitive sites in t(8;21) leukemiaYanming Zhang
Section of Hematology/Oncology, Department of Medicine, University of Chicago, Chicago, IL 60637, USA
Proc Natl Acad Sci U S A 99:3070-5. 2002..These sites correlated with genomic DNA breakpoints in both AML1 and ETO, thus implicating them in the de novo 8;21 translocation... - Meeting report: Seventh International Workshop on Molecular Aspects of Myeloid Stem Cell Development and Leukemia, Annapolis, MD, May 13-16, 2007Linda Wolff
National Cancer Institute, Bethesda, MD 20892, USA
Exp Hematol 36:523-32. 2008
Research Grants
- TEL, AML1, and TEL/AML1 in HematopoiesisGiuseppina Nucifora; Fiscal Year: 2006....
- Inactivating EVI1 for the Treatment of Myelodysplastic *Giuseppina Nucifora; Fiscal Year: 2007..The third aim will be focused on the role of arsenic trioxide in the treatment of EVI1-positive MDS and on the isolation of small molecules that inhibit EVI1. ..
- Role of EVI1 Modifications in Cell TransformationGiuseppina Nucifora; Fiscal Year: 2009..2. To identify the components and the functions of the nuclear EVI1 multiprotein complexes. 3. To characterize the sumoylation and acetylation of EVI1 in vivo and to define their role in EVI1- induced transformation. ..
- MOLECULAR ANALYSIS OF TEL AND TEL/AML1 IN PREB LEUKEMIAGiuseppina Nucifora; Fiscal Year: 2001..The experiments outlined in this proposal will allow them to begin to dissect the effects of TEL/AML1 in hematopoiesis and its contribution to leukogenesis. ..
- EVI1 Expression is a Prognostic Marker of CMLGiuseppina Nucifora; Fiscal Year: 2004..Real-time RT-PCR analysis and statistical analysis of CML and control samples will be used for this study. ..
- ANALYSIS OF MULTIPLE CHIMERIC TRANSCRIPTS IN THE T(3;21)Giuseppina Nucifora; Fiscal Year: 2004..In addition, our results will provide insight in the study of leukemias in which either AML 1 or MDS1/EVI1 are rearranged. ..
- INTERFERON ALPHA-INDUCED BIOLOGICAL RESPONSES IN CMLGiuseppina Nucifora; Fiscal Year: 2004..The ability to identify CML patients who will not respond to IFNalpha-therapy will be a very valuable clinical tool that could affect their treatment and their overall survival. ..
- A Mouse Model of Myelodysplastic Syndrome ProgressionGiuseppina Nucifora; Fiscal Year: 2009..We plan to use a combination of molecular biology and in vivo systems to dissect the molecular pathways of EVI1 and identify steps that can be used for the development of new treatments. ..