S F Nothwehr

Summary

Affiliation: University of Missouri
Country: USA

Publications

  1. ncbi Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p
    S F Nothwehr
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    J Cell Biol 151:297-310. 2000
  2. ncbi Distinct domains within Vps35p mediate the retrieval of two different cargo proteins from the yeast prevacuolar/endosomal compartment
    S F Nothwehr
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Mol Biol Cell 10:875-90. 1999
  3. ncbi The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi
    S F Nothwehr
    Division of Biological Sciences, University of Missouri, Columbia 65211, USA
    J Cell Sci 110:1063-72. 1997
  4. ncbi A novel mechanism for localizing membrane proteins to yeast trans-Golgi network requires function of synaptojanin-like protein
    S A Ha
    Division of Biological Sciences, University of Missouri, Columbia, 65211, USA
    Mol Biol Cell 12:3175-90. 2001
  5. ncbi The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins
    S F Nothwehr
    Division of Biological Sciences, University of Missouri, Columbia 65211, USA
    Mol Cell Biol 16:2700-7. 1996
  6. ncbi The clathrin adaptor complex 1 directly binds to a sorting signal in Ste13p to reduce the rate of its trafficking to the late endosome of yeast
    Christopher Foote
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    J Cell Biol 173:615-26. 2006
  7. ncbi Golgi-to-late endosome trafficking of the yeast pheromone processing enzyme Ste13p is regulated by a phosphorylation site in its cytosolic domain
    Holly D Johnston
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211
    Mol Biol Cell 16:1456-68. 2005
  8. ncbi Retrograde transport of the mannosyltransferase Och1p to the early Golgi requires a component of the COG transport complex
    Paul Bruinsma
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    J Biol Chem 279:39814-23. 2004
  9. ncbi The synaptojanin-like protein Inp53/Sjl3 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway
    Seon Ah Ha
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Mol Biol Cell 14:1319-33. 2003
  10. ncbi Structural features of vps35p involved in interaction with other subunits of the retromer complex
    Ricardo Restrepo
    Division of Biological Sciences, 401 Tucker Hall, University of Missouri, Columbia, MO 65211, USA
    Traffic 8:1841-53. 2007

Research Grants

  1. ANALYSIS OF GOLGI MEMBRANE PROTEIN LOCALIZATION IN YEAST
    Steven Nothwehr; Fiscal Year: 2003

Collaborators

  • D B DeWald
  • N J Bryant
  • PETER R ARVAN
  • Pietro V De Camilli
  • Christopher Foote
  • Ricardo Restrepo
  • Holly D Johnston
  • Paul Bruinsma
  • Seon Ah Ha
  • S A Ha
  • Harald Peter
  • Xiang Zhao
  • Bao Yan Zhang
  • Bao-yan Zhang
  • Andrea Santeford
  • Robert G Spelbrink
  • Javad Torabinejad
  • Markus R Wenk
  • Louise Lucast
  • Seon-Ah Ha
  • Ruedi Aebersold
  • Richard A Newitt
  • H Hama
  • J T Bunch

Detail Information

Publications11

  1. ncbi Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p
    S F Nothwehr
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    J Cell Biol 151:297-310. 2000
    ..These data strongly support a model in which Vps35p acts as a "receptor" protein for recognition of the retrieval signal domains of cargo proteins during their recruitment into retrograde vesicles...
  2. ncbi Distinct domains within Vps35p mediate the retrieval of two different cargo proteins from the yeast prevacuolar/endosomal compartment
    S F Nothwehr
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Mol Biol Cell 10:875-90. 1999
    ..These data suggest a model in which distinct structural features within Vps35p are required for associating with the cytosolic domains of each cargo protein during the retrieval process...
  3. ncbi The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi
    S F Nothwehr
    Division of Biological Sciences, University of Missouri, Columbia 65211, USA
    J Cell Sci 110:1063-72. 1997
    ..These results support a model in which Vps5p is required for retrieval of membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus...
  4. ncbi A novel mechanism for localizing membrane proteins to yeast trans-Golgi network requires function of synaptojanin-like protein
    S A Ha
    Division of Biological Sciences, University of Missouri, Columbia, 65211, USA
    Mol Biol Cell 12:3175-90. 2001
    ..Cell fractionation experiments suggested that Inp53p associates with Golgi or endosomal membranes. Taken together, these results suggest that a phosphoinositide signaling event regulates TGN-to-PVC transport of select cargo proteins...
  5. ncbi The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins
    S F Nothwehr
    Division of Biological Sciences, University of Missouri, Columbia 65211, USA
    Mol Cell Biol 16:2700-7. 1996
    ..All of the grd mutants exhibited significant mislocalization of A-ALP, as measured by determining the kinetics of A-ALP processing and by analyzing its..
  6. ncbi The clathrin adaptor complex 1 directly binds to a sorting signal in Ste13p to reduce the rate of its trafficking to the late endosome of yeast
    Christopher Foote
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    J Cell Biol 173:615-26. 2006
    ..These results suggest a model in which this novel sorting signal targets A(F-->A)-ALP into clathrin/AP-1 vesicles at the EE for retrieval back to the TGN...
  7. ncbi Golgi-to-late endosome trafficking of the yeast pheromone processing enzyme Ste13p is regulated by a phosphorylation site in its cytosolic domain
    Holly D Johnston
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211
    Mol Biol Cell 16:1456-68. 2005
    ..These results indicate that S13 phosphorylation is required for TGN-to-PVC trafficking of A-ALP and imply that phosphorylation of S13 may regulate recognition of A-ALP by vesicular trafficking machinery...
  8. ncbi Retrograde transport of the mannosyltransferase Och1p to the early Golgi requires a component of the COG transport complex
    Paul Bruinsma
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    J Biol Chem 279:39814-23. 2004
    ..However, Och1p does use a post-Golgi pathway for degradation because a portion of Och1p was degraded in the vacuole. Most surprisingly, Och1p can use either the carboxypeptidase Y or AP-3 pathways to reach the vacuole for degradation...
  9. ncbi The synaptojanin-like protein Inp53/Sjl3 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway
    Seon Ah Ha
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Mol Biol Cell 14:1319-33. 2003
    ..Taken together with other recent studies, our results suggest that Inp53p and AP-1/clathrin act together in a TGN-to-early endosome pathway distinct from the direct TGN-to-PVC pathway mediated by GGA/clathrin...
  10. ncbi Structural features of vps35p involved in interaction with other subunits of the retromer complex
    Ricardo Restrepo
    Division of Biological Sciences, 401 Tucker Hall, University of Missouri, Columbia, MO 65211, USA
    Traffic 8:1841-53. 2007
    ....
  11. ncbi A retention factor keeps death at bay
    Steven F Nothwehr
    Nat Cell Biol 5:281-3. 2003

Research Grants1

  1. ANALYSIS OF GOLGI MEMBRANE PROTEIN LOCALIZATION IN YEAST
    Steven Nothwehr; Fiscal Year: 2003
    ..Finally, several genes have been identified in genetic screens as defective in Golgi retention of proteins, and four of these will be investigated in further detail: grd20, grd21, grd22 and grd23. ..