VICTOR F NIZET

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc NOD2 contributes to cutaneous defense against Staphylococcus aureus through alpha-toxin-dependent innate immune activation
    Petr Hruz
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 106:12873-8. 2009
  2. pmc Staphylococcus aureus golden pigment impairs neutrophil killing and promotes virulence through its antioxidant activity
    George Y Liu
    Department of Pediatrics, University of California San Diego, La Jolla, CA 92093, USA
    J Exp Med 202:209-15. 2005
  3. pmc The surface-anchored NanA protein promotes pneumococcal brain endothelial cell invasion
    Satoshi Uchiyama
    Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA
    J Exp Med 206:1845-52. 2009
  4. pmc Transcription factor binding site analysis identifies FOXO transcription factors as regulators of the cutaneous wound healing process
    Karl Markus Roupé
    Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 9:e89274. 2014
  5. pmc Group B streptococcal beta-hemolysin/cytolysin directly impairs cardiomyocyte viability and function
    Mary E Hensler
    Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 3:e2446. 2008
  6. pmc Glycosaminoglycan binding facilitates entry of a bacterial pathogen into central nervous systems
    Yung Chi Chang
    Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California, United States of America
    PLoS Pathog 7:e1002082. 2011
  7. pmc Influences of chloride and hypochlorite on neutrophil extracellular trap formation
    Kathryn Akong-Moore
    Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 7:e42984. 2012
  8. pmc Innate immunity turned inside-out: antimicrobial defense by phagocyte extracellular traps
    Maren von Köckritz-Blickwede
    Department of Pediatrics, University of California, La Jolla, San Diego, CA 92093 0687, USA
    J Mol Med (Berl) 87:775-83. 2009
  9. pmc M1T1 group A streptococcal pili promote epithelial colonization but diminish systemic virulence through neutrophil extracellular entrapment
    Laura E Crotty Alexander
    Department of Pediatrics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    J Mol Med (Berl) 88:371-81. 2010
  10. pmc Study of the IgG endoglycosidase EndoS in group A streptococcal phagocyte resistance and virulence
    Jonathan Sjögren
    Division of Infection Medicine, Department of Clinical Sciences, Lund University, SE 221 84 Lund, Sweden
    BMC Microbiol 11:120. 2011

Collaborators

Detail Information

Publications72

  1. pmc NOD2 contributes to cutaneous defense against Staphylococcus aureus through alpha-toxin-dependent innate immune activation
    Petr Hruz
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 106:12873-8. 2009
    ....
  2. pmc Staphylococcus aureus golden pigment impairs neutrophil killing and promotes virulence through its antioxidant activity
    George Y Liu
    Department of Pediatrics, University of California San Diego, La Jolla, CA 92093, USA
    J Exp Med 202:209-15. 2005
    ..Blocking S. aureus carotenogenesis increases oxidant sensitivity and decreases whole-blood survival, suggesting a novel target for antibiotic therapy...
  3. pmc The surface-anchored NanA protein promotes pneumococcal brain endothelial cell invasion
    Satoshi Uchiyama
    Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA
    J Exp Med 206:1845-52. 2009
    ..NanA promotes SPN-BBB interaction in a murine infection model, identifying the protein as proximal mediator of CNS entry by the pathogen...
  4. pmc Transcription factor binding site analysis identifies FOXO transcription factors as regulators of the cutaneous wound healing process
    Karl Markus Roupé
    Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 9:e89274. 2014
    ..FOXO3 has not previously been implicated as an important regulator of wound healing and its exact function in this process calls for further investigation. ..
  5. pmc Group B streptococcal beta-hemolysin/cytolysin directly impairs cardiomyocyte viability and function
    Mary E Hensler
    Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 3:e2446. 2008
    ..Here we study the effects of the GBS pore-forming beta-hemolysin/cytolysin (Bh/c) exotoxin on cardiomyocyte viability, contractility, and calcium transients...
  6. pmc Glycosaminoglycan binding facilitates entry of a bacterial pathogen into central nervous systems
    Yung Chi Chang
    Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California, United States of America
    PLoS Pathog 7:e1002082. 2011
    ..Our results also highlight how the simpler yet genetically conserved Drosophila GAG pathways can provide a model organism to screen candidate molecules that can interrupt pathogen-GAG interactions for future therapeutic applications...
  7. pmc Influences of chloride and hypochlorite on neutrophil extracellular trap formation
    Kathryn Akong-Moore
    Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 7:e42984. 2012
    ..To better understand the roles of various ROS in NETosis, we explore the role of MPO, its substrates chloride ion (Cl(-)) and hydrogen peroxide (H(2)O(2)), and its product hypochlorite (HOCl) in NETosis...
  8. pmc Innate immunity turned inside-out: antimicrobial defense by phagocyte extracellular traps
    Maren von Köckritz-Blickwede
    Department of Pediatrics, University of California, La Jolla, San Diego, CA 92093 0687, USA
    J Mol Med (Berl) 87:775-83. 2009
    ..Attention is also given to mechanisms by which certain leading bacterial pathogens have evolved to avoid entrapment and killing in these specialized structures...
  9. pmc M1T1 group A streptococcal pili promote epithelial colonization but diminish systemic virulence through neutrophil extracellular entrapment
    Laura E Crotty Alexander
    Department of Pediatrics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    J Mol Med (Berl) 88:371-81. 2010
    ....
  10. pmc Study of the IgG endoglycosidase EndoS in group A streptococcal phagocyte resistance and virulence
    Jonathan Sjögren
    Division of Infection Medicine, Department of Clinical Sciences, Lund University, SE 221 84 Lund, Sweden
    BMC Microbiol 11:120. 2011
    ..In this study we couple targeted allelic replacement mutagenesis and heterologous expression to elucidate the contribution of EndoS to group A Streptococcus (GAS) phagocyte resistance and pathogenicity in vitro and in vivo...
  11. pmc The GraRS regulatory system controls Staphylococcus aureus susceptibility to antimicrobial host defenses
    Dirk Kraus
    Cellular and Molecular Microbiology Division, Institute for Medical Microbiology and Hygiene, University of Tubingen, Elfriede Aulhorn Str, 6, 72076 Tubingen, Germany
    BMC Microbiol 8:85. 2008
    ..The graRS regulatory genes have recently been implicated in the control of D-alanylation in Staphylococcus aureus...
  12. ncbi request reprint Understanding how leading bacterial pathogens subvert innate immunity to reveal novel therapeutic targets
    Victor Nizet
    Division of Pediatric Pharmacology and Drug Discovery, University of California, San Diego School of Medicine and Skaggs School of Pharmacy and Pharmaceutical Sciences, La Jolla, CA 92093 0687, USA
    J Allergy Clin Immunol 120:13-22. 2007
    ..These future therapies envision alternatives to direct microbial killing, such as blocking disease progression by neutralizing specific virulence factors or boosting key innate immune defenses...
  13. pmc Invasion of brain microvascular endothelial cells by group B streptococci
    V Nizet
    Division of Infectious Diseases, Children s Hospital and Medical Center, Seattle, Washington 98105, USA
    Infect Immun 65:5074-81. 1997
    ..GBS invasion of BMEC may be a primary step in the pathogenesis of meningitis, allowing bacteria access to the CNS by transcytosis or by injury and disruption of the endothelial blood-brain barrier...
  14. ncbi request reprint Interdependence of hypoxic and innate immune responses
    Victor Nizet
    Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nat Rev Immunol 9:609-17. 2009
    ..In turn, HIF-driven innate immune responses have important consequences for both the pathogen and the host, such that the tissue microenvironment fundamentally influences susceptibility to infectious disease...
  15. ncbi request reprint Antimicrobial peptide resistance mechanisms of human bacterial pathogens
    Victor Nizet
    Department of Pediatrics, Division of Infectious Diseases, University of California, San Diego, 9500 Gilman Drive, MC 0687, La Jolla, CA 92093 0687, USA
    Curr Issues Mol Biol 8:11-26. 2006
    ..Knowledge of the molecular basis of bacterial AMP resistance may provide new targets for antimicrobial therapy of human infectious diseases...
  16. ncbi request reprint Cathelicidins and innate defense against invasive bacterial infection
    Victor Nizet
    Department of Pediatrics, Division of Infectious Diseases University of California, San Diego, La Jolla 92093, USA
    Scand J Infect Dis 35:670-6. 2003
    ..This article reviews the latest advances in the knowledge of cathelicidin antimicrobial peptides, with particular emphasis on their role in defense against invasive bacterial infection and associations with human disease conditions...
  17. ncbi request reprint Streptococcal beta-hemolysins: genetics and role in disease pathogenesis
    Victor Nizet
    Division of Pediatric Infectious Diseases, University of California, San Diego, 9500 Gilman Drive, MC 0672, La Jolla 92093, USA
    Trends Microbiol 10:575-80. 2002
    ..The generation of isogenic GAS and GBS beta-hemolysin-deficient mutants and their analysis using in vitro and in vivo model systems has shown that both toxins function as virulence factors in the pathogenesis of invasive infections...
  18. ncbi request reprint Streptolysin S and necrotising infections produced by group G streptococcus
    Deepali Humar
    Department of Medicine, Division of Infectious Diseases, University of California, San Diego 92093, USA
    Lancet 359:124-9. 2002
    ..Due to strong clinical similarities with invasive infections produced by group A streptococcus, we investigated a potential link of shared beta-haemolytic phenotype to disease pathogenesis...
  19. ncbi request reprint Streptococcus iniae beta-hemolysin streptolysin S is a virulence factor in fish infection
    Jeffrey B Locke
    Department of Pediatrics, Division of Pharmacology and Drug Discovery, University of California, San Diego, 9500 Gilman Drive, MC 0687, La Jolla, California 92093 0687, USA
    Dis Aquat Organ 76:17-26. 2007
    ..The neutralization of SLS could represent a novel strategy for control of S. iniae infection in aquaculture...
  20. pmc D-alanylation of teichoic acids promotes group a streptococcus antimicrobial peptide resistance, neutrophil survival, and epithelial cell invasion
    Sascha A Kristian
    Division of Pediatric Infectious Diseases, University of California, San Diego School of Medicine, Cellular and Molecular Medicine East, Room 1066, 9500 Gilman Drive, Mail Code 0687, La Jolla, CA 92093 0687, USA
    J Bacteriol 187:6719-25. 2005
    ..Thus, teichoic acid d-alanylation may contribute in multiple ways to the propensity of invasive GAS to bypass mucosal defenses and produce systemic infection...
  21. pmc The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae
    Carlo J E Milani
    Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, MC 0687, La Jolla, CA 92093 0687, USA
    Microbiology 156:543-54. 2010
    ..iniae. Identification of Pdi and other S. iniae virulence proteins is a necessary initial step towards the development of appropriate preventive and therapeutic measures against diseases and economic losses caused by this pathogen...
  22. pmc Sword and shield: linked group B streptococcal beta-hemolysin/cytolysin and carotenoid pigment function to subvert host phagocyte defense
    George Y Liu
    Department of Pediatrics, The Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 101:14491-6. 2004
    ..Together, these data suggest a mechanism by which the linked cylE-encoded phenotypes, betaH/C (sword) and carotenoid (shield), act in partnership to thwart the immune phagocytic defenses...
  23. pmc The IL-8 protease SpyCEP/ScpC of group A Streptococcus promotes resistance to neutrophil killing
    Annelies S Zinkernagel
    Department of Pediatrics, Division of Pharmacology and Drug Discovery, La Jolla, CA 92093, USA
    Cell Host Microbe 4:170-8. 2008
    ..By inactivating the multifunctional host defense peptide IL-8, the SpyCEP protease impairs neutrophil clearance mechanisms, contributing to the pathogenesis of invasive streptococcal infection...
  24. pmc Recent advances in understanding the molecular basis of group B Streptococcus virulence
    Heather C Maisey
    Department of Pediatrics, University of California San Diego, La Jolla, CA 92093 0687, USA
    Expert Rev Mol Med 10:e27. 2008
    ..An understanding of the complex interplay between Group B Streptococcus and host provides valuable insight into pathogen evolution and highlights molecular targets for therapeutic intervention...
  25. pmc The group B streptococcal serine-rich repeat 1 glycoprotein mediates penetration of the blood-brain barrier
    Nina M van Sorge
    Department of Pediatrics and 2Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, San Diego, CA 92182, USA
    J Infect Dis 199:1479-87. 2009
    ..Because GBS is able to invade, survive, and cross the blood-brain barrier, we sought to identify surface-expressed virulence factors that contribute to blood-brain barrier penetration and the pathogenesis of meningitis...
  26. pmc ClpX contributes to innate defense peptide resistance and virulence phenotypes of Bacillus anthracis
    Shauna M McGillivray
    Department of Pediatrics, University of California San Diego, La Jolla, CA 92093 0687, USA
    J Innate Immun 1:494-506. 2009
    ..We conclude that ClpX is an important factor allowing B. anthracis to subvert host immune clearance mechanisms, and thus represents a novel therapeutic target for prevention or therapy of anthrax, a foremost biodefense concern...
  27. pmc HIF-1alpha expression regulates the bactericidal capacity of phagocytes
    Carole Peyssonnaux
    Division of Biological Sciences, Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA
    J Clin Invest 115:1806-15. 2005
    ..HIF-1alpha control of myeloid cell activity in infected tissues could represent a novel therapeutic target for enhancing host defense...
  28. pmc A group B streptococcal pilus protein promotes phagocyte resistance and systemic virulence
    Heather C Maisey
    Department of Pediatrics, Division of Pharmacology and Drug Discovery, University of California, San Diego, School of Medicine, La Jolla, California, USA
    FASEB J 22:1715-24. 2008
    ..Together these data demonstrate that the pilus backbone subunit, PilB, plays an integral role in GBS virulence and suggests a novel role for gram-positive pili in thwarting the innate defenses of phagocyte killing...
  29. ncbi request reprint Mutational analysis of the group A streptococcal operon encoding streptolysin S and its virulence role in invasive infection
    Vivekanand Datta
    Department of Pediatrics, Division of Infectious Diseases, University of California, San Diego, La Jolla, CA, USA
    Mol Microbiol 56:681-95. 2005
    ..We conclude that all genetic components of the sag operon are required for expression of functional SLS, an important virulence factor in the pathogenesis of invasive M1T1 GAS infection...
  30. pmc CAMP factor is not essential for systemic virulence of Group B Streptococcus
    Mary E Hensler
    Division of Pediatric Pharmacology and Drug Discovery, School of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0687, USA
    Microb Pathog 44:84-8. 2008
    ..Our data suggest that CAMP factor expressed in its native context is not essential for systemic virulence of GBS...
  31. pmc Clostridiolysin S, a post-translationally modified biotoxin from Clostridium botulinum
    David J Gonzalez
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:28220-8. 2010
    ..is responsible for the biogenesis of a novel post-translationally modified hemolytic toxin, clostridiolysin S...
  32. pmc Streptococcus iniae capsule impairs phagocytic clearance and contributes to virulence in fish
    Jeffrey B Locke
    Department of Pediatrics, Division of Pharmacology and Drug Discovery, University of California, San Diego, School of Medicine, La Jolla, CA 92093, USA
    J Bacteriol 189:1279-87. 2007
    ..In summary, we report a genetically defined study on the role of capsule in S. iniae virulence and provide preliminary analysis of S. iniae capsular polysaccharide sugar components...
  33. ncbi request reprint Discovery and characterization of two isoforms of moronecidin, a novel antimicrobial peptide from hybrid striped bass
    Xavier Lauth
    Department of Pediatrics, University of California, San Diego School of Medicine, La Jolla, California 92093 0830, USA
    J Biol Chem 277:5030-9. 2002
    ..Thus, moronecidin is a new alpha-helical, broad spectrum antimicrobial peptide isolated from the skin and gills of hybrid striped bass...
  34. pmc Penicillin-binding protein 1a promotes resistance of group B streptococcus to antimicrobial peptides
    Andrea Hamilton
    Department of Pediatrics, University of Washington, and Children s Hospital and Regional Medical Center, 307 Westlake Ave N, Suite 300, Seattle, WA 98109, USA
    Infect Immun 74:6179-87. 2006
    ..While the peptidoglycan polymerization and cross-linking activity of PBPs are essential for bacterial survival, our study is the first to identify a role for a PBP in resistance to host AMPs...
  35. doi request reprint Pharmacologic augmentation of hypoxia-inducible factor-1alpha with mimosine boosts the bactericidal capacity of phagocytes
    Annelies S Zinkernagel
    1Division of Pediatric Pharmacology and Drug Discovery, School of Medicine, University of California, San Diego, La Jolla 92093 0687, USA
    J Infect Dis 197:214-7. 2008
    ..aureus skin infection. This provides the first proof of principle for a novel approach to the treatment of bacterial infection by pharmacologically augmenting the host phagocytic function...
  36. ncbi request reprint HIF-1alpha is essential for myeloid cell-mediated inflammation
    Thorsten Cramer
    Molecular Biology Section, Division of Biological Sciences, School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 112:645-57. 2003
    ..This role for HIF-1alpha demonstrates its direct regulation of survival and function in the inflammatory microenvironment...
  37. pmc M1 protein allows Group A streptococcal survival in phagocyte extracellular traps through cathelicidin inhibition
    Xavier Lauth
    Department of Pediatrics, University of California San Diego, La Jolla, Calif 92093 0687, USA
    J Innate Immun 1:202-14. 2009
    ..We conclude increased resistance to host cathelicidin and killing within phagocyte extracellular traps contribute to the propensity of M1 GAS strains to produce invasive infections...
  38. doi request reprint Cell death during sepsis: integration of disintegration in the inflammatory response to overwhelming infection
    Fabiano Pinheiro da Silva
    Department of Pediatrics, School of Medicine, University of California San Diego, La Jolla, CA 92093 0687, USA
    Apoptosis 14:509-21. 2009
    ..A greater knowledge of cell death pathways in sepsis informs the potential for future therapies designed to ameliorate immune dysfunction in this syndrome...
  39. ncbi request reprint IKKalpha limits macrophage NF-kappaB activation and contributes to the resolution of inflammation
    Toby Lawrence
    Laboratory of Gene Regulation and Signal Transduction, Department of Pharmacology, University of California San Diego, 9500 Gilman Drive, California 92093, USA
    Nature 434:1138-43. 2005
    ..Inactivation of IKKalpha in mice enhances inflammation and bacterial clearance. Hence, the two IKK catalytic subunits have evolved opposing but complimentary roles needed for the intricate control of inflammation and innate immunity...
  40. pmc Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5
    Aaron F Carlin
    Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA
    J Exp Med 206:1691-9. 2009
    ..We conclude that protein-mediated functional engagement of an inhibitory host lectin receptor promotes bacterial innate immune evasion...
  41. doi request reprint Evaluation of Streptococcus iniae killed bacterin and live attenuated vaccines in hybrid striped bass through injection and bath immersion
    Jeffrey B Locke
    Department of Pediatrics, Division of Pharmacology and Drug Discovery, University of California, San Diego, 9500 Gilman Drive, MC 0687, La Jolla, California 92093 0687, USA
    Dis Aquat Organ 89:117-23. 2010
    ..Our studies demonstrate the efficacy of live attenuated vaccines for prevention of S. iniae infection, and identify immersion delivery of live vaccines as an attractive option for use in commercial aquaculture settings...
  42. ncbi request reprint Cellular activation, phagocytosis, and bactericidal activity against group B streptococcus involve parallel myeloid differentiation factor 88-dependent and independent signaling pathways
    Philipp Henneke
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Immunol 169:3970-7. 2002
    ..We conclude that the inflammatory, bactericidal, and phagocytic responses to GBS occur via parallel but independent processes...
  43. ncbi request reprint Molecular pathogenesis of neonatal group B streptococcal infection: no longer in its infancy
    Kelly S Doran
    Department of Pediatrics, Division of Infectious Diseases, University of California, San Diego School of Medicine, La Jolla, CA 92093, USA
    Mol Microbiol 54:23-31. 2004
    ..Appreciation of the formidable array of GBS virulence factors underscores why this bacterium remains at the forefront of neonatal pathogens...
  44. ncbi request reprint Endogenous production of antimicrobial peptides in innate immunity and human disease
    Richard L Gallo
    Departments of Medicine and Pediatrics, University of California San Diego, San Diego, CA, USA
    Curr Allergy Asthma Rep 3:402-9. 2003
    ..In this article, we review important recent advances in understanding the biology of antimicrobial peptides and their role in normal immunity and human disease...
  45. ncbi request reprint Virulence role of group B Streptococcus beta-hemolysin/cytolysin in a neonatal rabbit model of early-onset pulmonary infection
    Mary E Hensler
    Department of Pediatrics, Division of Infectious Diseases, School of Medicine, University of California at San Diego, La Jolla, California 92093 0687, USA
    J Infect Dis 191:1287-91. 2005
    ..This work, to our knowledge, provides the first in vivo evidence for a critical role of the beta h/c toxin in GBS neonatal pneumonia and in the breakdown of the pulmonary barrier to systemic infection...
  46. pmc Group B streptococcal beta-hemolysin/cytolysin activates neutrophil signaling pathways in brain endothelium and contributes to development of meningitis
    Kelly S Doran
    Department of Pediatrics, University of California, San Diego, School of Medicine, La Jolla, California 92093 0672, USA
    J Clin Invest 112:736-44. 2003
    ..In a murine model of hematogenous meningitis, mice infected with beta-h/c mutants exhibited lower mortality and decreased brain bacterial counts compared with mice infected with the corresponding WT GBS strains...
  47. pmc Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin
    Anna L Cogen
    Division of Dermatology, Department of Medicine, University of California San Diego, VA San Diego Health Care System, San Diego, California, USA
    J Invest Dermatol 130:192-200. 2010
    ..Thus, these data suggest that the production of PSMgamma and PSMdelta by S. epidermidis can benefit cutaneous immune defense by selectively inhibiting the survival of skin pathogens while maintaining the normal skin microbiome...
  48. pmc IKKbeta/NF-kappaB and the miscreant macrophage
    Anjuli M Timmer
    Department of Pediatrics, School of Medicine, and Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, USA
    J Exp Med 205:1255-9. 2008
    ..As cancers and pathogens may have evolved multiple mechanisms to manipulate macrophages for their own survival, is there anything we can do about it?..
  49. ncbi request reprint How group A Streptococcus circumvents host phagocyte defenses
    Laura A Kwinn
    Division of Pediatric Pharmacology and Drug Discovery, University of California, San Diego School of Medicine, La Jolla, CA 92093, USA
    Future Microbiol 2:75-84. 2007
    ..Understanding the molecular basis of GAS phagocyte resistance may reveal novel therapeutic targets for treatment and prevention of invasive human infections...
  50. pmc Blood-brain barrier invasion by group B Streptococcus depends upon proper cell-surface anchoring of lipoteichoic acid
    Kelly S Doran
    Department of Pediatrics, Division of Infectious Diseases, UCSD School of Medicine, La Jolla, California 92093, USA
    J Clin Invest 115:2499-507. 2005
    ..Our data suggest that LTA expression on the GBS surface plays a role in bacterial interaction with BBB endothelium and the pathogenesis of neonatal meningitis...
  51. pmc Genetic characterization and virulence role of the RALP3/LSA locus upstream of the streptolysin s operon in invasive M1T1 Group A Streptococcus
    Laura A Kwinn
    Department of Pediatrics, Division of Pharmacology and Drug Discovery, University of California, San Diego School of Medicine, Cellular and Molecular Medicine East, La Jolla, CA 92093 0687, USA
    J Bacteriol 189:1322-9. 2007
    ..Isogenic M1T1 GAS mutants lacking either RALP3 or LSA were attenuated in a murine model of systemic infection, indicating that this locus plays a role in the virulence potential of the organism...
  52. pmc Brain abscess caused by Streptococcus pyogenes in a previously healthy child
    Walter Dehority
    Division of Pediatric Infectious Diseases, School of Medicine, University of California, San Diego, and Rady Children s Hospital and Health Center, San Diego, 3020 Children s Way, MC 5041, San Diego, CA 92123, USA
    J Clin Microbiol 44:4613-5. 2006
    ..We report the case of a previously healthy boy with brain abscesses caused by M/emm type 12 GAS and review the case in the context of the published literature and recent epidemiological data...
  53. pmc Keratinocyte production of cathelicidin provides direct activity against bacterial skin pathogens
    Marissa H Braff
    Department of Medicine, Department of Pediatrics, University of California, San Diego, USA
    Infect Immun 73:6771-81. 2005
    ....
  54. ncbi request reprint Strain-associated virulence factors of Streptococcus iniae in hybrid-striped bass
    John T Buchanan
    Department of Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA
    Vet Microbiol 131:145-53. 2008
    ..Our results indicate that resistance to phagocytosis, oxidative killing, and associated phagocytic clearance is a significant factor in S. iniae virulence...
  55. ncbi request reprint The mammalian ionic environment dictates microbial susceptibility to antimicrobial defense peptides
    Robert A Dorschner
    Division of Dermatology, Department of Medicine, University of California San Diego and VA San Diego Healthcare Center, San Diego, USA
    FASEB J 20:35-42. 2006
    ....
  56. pmc Streptococcus iniae phosphoglucomutase is a virulence factor and a target for vaccine development
    John T Buchanan
    Department of Pediatrics, University of California, San Diego, School of Medicine, La Jolla, 92093, USA
    Infect Immun 73:6935-44. 2005
    ..An S. iniae DeltaPGM mutant is able to stimulate a protective immune response and may have value as a live attenuated vaccine for aquaculture...
  57. ncbi request reprint Innate immunity gone awry: linking microbial infections to chronic inflammation and cancer
    Michael Karin
    Laboratory of Gene Expression and Signal Transduction, Department of Pharmacology, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Cell 124:823-35. 2006
    ....
  58. ncbi request reprint DNase expression allows the pathogen group A Streptococcus to escape killing in neutrophil extracellular traps
    John T Buchanan
    Department of Pediatrics, University of California, San Diego, La Jolla, California 92093, USA
    Curr Biol 16:396-400. 2006
    ..The results demonstrate a significant role for NETs in neutrophil-mediated innate immunity, and at the same time identify a novel therapeutic target against invasive GAS infection...
  59. pmc Group B streptococcal pilus proteins contribute to adherence to and invasion of brain microvascular endothelial cells
    Heather C Maisey
    Department of Pediatrics, Division of Pharmacology and Drug Discovery, University of California, San Diego School of Medicine, La Jolla, CA 92093, USA
    J Bacteriol 189:1464-7. 2007
    ..We show that two GBS proteins involved in pilus formation, encoded by pilA and pilB, also facilitate the interaction of this important agent of central nervous system infection with endothelial cells of the human blood-brain barrier...
  60. pmc Regulation of iron homeostasis by the hypoxia-inducible transcription factors (HIFs)
    Carole Peyssonnaux
    Molecular Biology Section, Division of Biological Sciences, UCSD School of Medicine, La Jolla, CA 92093 0377, USA
    J Clin Invest 117:1926-32. 2007
    ..Through coordinate downregulation of hepcidin and upregulation of erythropoietin and ferroportin, the VHL-HIF pathway mobilizes iron to support erythrocyte production...
  61. pmc A toll-like receptor 2-responsive lipid effector pathway protects mammals against skin infections with gram-positive bacteria
    Philippe Georgel
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Infect Immun 73:4512-21. 2005
    ..These observations reveal the existence of a regulated, lipid-based antimicrobial effector pathway in mammals and suggest new approaches to the treatment or prevention of infections with gram-positive bacteria...
  62. ncbi request reprint Bass hepcidin synthesis, solution structure, antimicrobial activities and synergism, and in vivo hepatic response to bacterial infections
    Xavier Lauth
    Department of Pediatrics, University of California San Diego, School of Medicine, San Diego, California 92093, USA
    J Biol Chem 280:9272-82. 2005
    ..Our results suggest that hepcidin plays a key role in the antimicrobial defenses of bass and that its functions are potentially conserved between fish and human...
  63. ncbi request reprint Serum opacity factor promotes group A streptococcal epithelial cell invasion and virulence
    Anjuli M Timmer
    Department of Pediatrics, Division of Pharmacology and Drug Discovery, University of California, San Diego, La Jolla, CA, USA
    Mol Microbiol 62:15-25. 2006
    ..Finally, the first in vivo studies to employ a single gene allelic replacement mutant of SOF demonstrate that this protein contributes to GAS virulence in a murine model of necrotizing skin infection...
  64. pmc Streptolysin O promotes group A Streptococcus immune evasion by accelerated macrophage apoptosis
    Anjuli M Timmer
    Department of Pediatrics, Biomedical Sciences Graduate Program, Laboratory of Signal Transduction, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 284:862-71. 2009
    ..We conclude that accelerated, caspase-dependent macrophage apoptosis induced by the pore-forming cytolysin SLO contributes to GAS immune evasion and virulence...
  65. pmc The Ashwell receptor mitigates the lethal coagulopathy of sepsis
    Prabhjit K Grewal
    The Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine University of California, San Diego, La Jolla, California 92093, USA
    Nat Med 14:648-55. 2008
    ..Hemostatic adaptation by the Ashwell receptor moderates the onset and severity of disseminated intravascular coagulation during sepsis and improves the probability of host survival...
  66. pmc Structural and functional dissection of the heterocyclic peptide cytotoxin streptolysin S
    Douglas A Mitchell
    Department of Pharmacology, University of California San Diego, La Jolla, CA 92093, USA
    J Biol Chem 284:13004-12. 2009
    ..This new structural information has ramifications for future antimicrobial therapies...
  67. doi request reprint Critical role of HIF-1alpha in keratinocyte defense against bacterial infection
    Carole Peyssonnaux
    Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0377, USA
    J Invest Dermatol 128:1964-8. 2008
    ..RNA interference studies reveal that HIF-1alpha regulation of keratinocyte cathelicidin production is critical to their antibacterial function...
  68. pmc Discovery of a widely distributed toxin biosynthetic gene cluster
    Shaun W Lee
    Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 105:5879-84. 2008
    ..Given our findings, it is likely that the discovery of similar peptidic toxins will rapidly expand to existing and emerging genomes...
  69. ncbi request reprint Hypoxia inducible factor (HIF) function in innate immunity and infection
    Annelies S Zinkernagel
    Division of Pediatric Pharmacology and Drug Discovery, University of California, San Diego School of Medicine, Cellular and Molecular Medicine East, Room 1066, 9500 Gilman Drive, MC 0687, La Jolla, CA 92093 0687, USA
    J Mol Med (Berl) 85:1339-46. 2007
    ..A better understanding of HIF-1alpha function may provide novel and rational approaches for boosting innate immune function in the therapy of certain complicated infectious disease conditions...
  70. pmc TLR4-dependent hepcidin expression by myeloid cells in response to bacterial pathogens
    Carole Peyssonnaux
    Division of Pediatric Infectious Diseases, Cellular and Molecular Medicine East, Rm 1066, University of California at San Diego School of Medicine, 9500 Gilman Dr, MC 0687, La Jolla, 92093 0687, USA
    Blood 107:3727-32. 2006
    ..Our findings identify endogenous myeloid cell hepcidin production as a previously unrecognized component of the host response to bacterial pathogens...

Research Grants14

  1. SLS: Molecular Basis and Role in Invasive GAS Disease
    Victor Nizet; Fiscal Year: 2002
    ..These hypotheses will be tested by molecular genetic studies, attempts protein purification and antibody development, and the use of targeted SLS mutants in in vitro assays of phagocytic function and our in vivo mouse model of GAS NF. ..
  2. GAS Switch from Colonizing Bacterium to Invasive Pathogen
    Victor Nizet; Fiscal Year: 2010
    ..We are studying the ways in which the GAS bacteria shifts from an innocent member of our normal flora to an invasive pathogen, using molecular genetic techniques, assays of immune function, and mouse models of infection. ..
  3. Sialic Acid O-Acetylation in GBS Pathogenesis & Immunity
    Victor Nizet; Fiscal Year: 2010
    ..Companion analyses will be performed on GBS isolates and human sera from recent prospective seroepidemiologic studies. ..
  4. GAS Switch from Colonizing Bacterium to Invasive Pathogen
    Victor Nizet; Fiscal Year: 2009
    ..We are studying the ways in which the GAS bacteria shifts from an innocent member of our normal flora to an invasive pathogen, using molecular genetic techniques, assays of immune function, and mouse models of infection. ..
  5. Sialic Acid O-Acetylation in GBS Pathogenesis & Immunity
    Victor Nizet; Fiscal Year: 2009
    ..Companion analyses will be performed on GBS isolates and human sera from recent prospective seroepidemiologic studies. ..
  6. Sialic Acid O-Acetylation in GBS Pathogenesis & Immunity
    Victor Nizet; Fiscal Year: 2007
    ..Companion analyses will be performed on GBS isolates and human sera from recent prospective seroepidemiologic studies. ..
  7. SLS: Molecular Basis and Role in Invasive GAS Disease
    Victor Nizet; Fiscal Year: 2006
    ..These hypotheses will be tested by molecular genetic studies, attempts protein purification and antibody development, and the use of targeted SLS mutants in in vitro assays of phagocytic function and our in vivo mouse model of GAS NF. ..
  8. Sialic Acid O-Acetylation in GBS Pathogenesis & Immunity
    Victor Nizet; Fiscal Year: 2006
    ..Companion analyses will be performed on GBS isolates and human sera from recent prospective seroepidemiologic studies. ..
  9. SLS: Molecular Basis and Role in Invasive GAS Disease
    Victor Nizet; Fiscal Year: 2005
    ..These hypotheses will be tested by molecular genetic studies, attempts protein purification and antibody development, and the use of targeted SLS mutants in in vitro assays of phagocytic function and our in vivo mouse model of GAS NF. ..
  10. SLS: Molecular Basis and Role in Invasive GAS Disease
    Victor Nizet; Fiscal Year: 2004
    ..These hypotheses will be tested by molecular genetic studies, attempts protein purification and antibody development, and the use of targeted SLS mutants in in vitro assays of phagocytic function and our in vivo mouse model of GAS NF. ..
  11. SLS: Molecular Basis and Role in Invasive GAS Disease
    Victor Nizet; Fiscal Year: 2003
    ..These hypotheses will be tested by molecular genetic studies, attempts protein purification and antibody development, and the use of targeted SLS mutants in in vitro assays of phagocytic function and our in vivo mouse model of GAS NF. ..
  12. Sialic Acid O-Acetylation in GBS Pathogenesis & Immunity
    Victor Nizet; Fiscal Year: 2009
    ..Companion analyses will be performed on GBS isolates and human sera from recent prospective seroepidemiologic studies. ..