Douglas Nixon

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Rapid progressing allele HLA-B35 Px restricted anti-HIV-1 CD8+ T cells recognize vestigial CTL epitopes
    Christian B Willberg
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 5:e10249. 2010
  2. pmc Influence of HAART on alternative reading frame immune responses over the course of HIV-1 infection
    Stephane Champiat
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 7:e39311. 2012
  3. pmc Decay Kinetics of HIV-1 Specific T Cell Responses in Vertically HIV-1 Exposed Seronegative Infants
    Sara J Holditch
    Division of Experimental Medicine, Department of Medicine, San Francisco General Hospital, University of California San Francisco San Francisco, CA, USA
    Front Immunol 2:94. 2011
  4. pmc Association of differentiation state of CD4+ T cells and disease progression in HIV-1 perinatally infected children
    Elizabeth R Sharp
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 7:e29154. 2012
  5. pmc Immunodominance of HIV-1 specific CD8+ T-cell responses is related to disease progression rate in vertically infected adolescents
    Elizabeth R Sharp
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 6:e21135. 2011
  6. pmc Increased expression of intrinsic antiviral genes in HLA-B*57-positive individuals
    Rui André Saraiva Raposo
    2 Division of Experimental Medicine, 1001 Potrero Ave, Bldg 3, Room 603, Box 1234, San Francisco, CA 94110, USA
    J Leukoc Biol 94:1051-9. 2013
  7. pmc Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques
    Karin J Metzner
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, NY 10016, USA
    Retrovirology 3:97. 2006
  8. pmc The frequency of CD127low expressing CD4+CD25high T regulatory cells is inversely correlated with human T lymphotrophic virus type-1 (HTLV-1) proviral load in HTLV-1-infection and HTLV-1-associated myelopathy/tropical spastic paraparesis
    Jakob Michaelsson
    Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA
    BMC Immunol 9:41. 2008
  9. pmc T cell responses to human endogenous retroviruses in HIV-1 infection
    Keith E Garrison
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS Pathog 3:e165. 2007
  10. ncbi request reprint Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells
    Annika C Karlsson
    Gladstone Institute of Virology and Immunology, University of California San Francisco, P O Box 419100, San Francisco, CA 94141 9100, USA
    J Immunol Methods 283:141-53. 2003

Research Grants

  1. DYNAMICS OF HIV1 SPECIFIC CTL IN HIV1 INFECTION
    Douglas Nixon; Fiscal Year: 2001
  2. Viral Tropism and Immune Control in HIV
    Douglas Nixon; Fiscal Year: 2006
  3. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2005
  4. T Regulatory Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2005
  5. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2004
  6. T Regulatory Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2006
  7. T Regulatory Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2009
  8. DYNAMICS OF HIV1 SPECIFIC CTL IN HIV1 INFECTION
    Douglas Nixon; Fiscal Year: 2003
  9. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2002
  10. HIV VARIATION AND CROSS CLADE CTL RECOGNITION
    Douglas Nixon; Fiscal Year: 2002

Detail Information

Publications73

  1. pmc Rapid progressing allele HLA-B35 Px restricted anti-HIV-1 CD8+ T cells recognize vestigial CTL epitopes
    Christian B Willberg
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 5:e10249. 2010
    ..The HLA-B*35-Px allele has been associated with rapid disease progression in HIV-1 infection, in contrast to the HLA-B*35-Py allele...
  2. pmc Influence of HAART on alternative reading frame immune responses over the course of HIV-1 infection
    Stephane Champiat
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 7:e39311. 2012
    ..Since the mutational pressure exerted on one location of the HIV-1 genome can potentially affect the 3 reading frames, we hypothesized that ARF responses would be affected by this drug pressure in vivo...
  3. pmc Decay Kinetics of HIV-1 Specific T Cell Responses in Vertically HIV-1 Exposed Seronegative Infants
    Sara J Holditch
    Division of Experimental Medicine, Department of Medicine, San Francisco General Hospital, University of California San Francisco San Francisco, CA, USA
    Front Immunol 2:94. 2011
    ..The disparate rate of decay with studies of adults placed on antiretroviral drugs suggests that antigen specific T cell responses are driven by the natural rate of decay of the T cell sub-populations themselves...
  4. pmc Association of differentiation state of CD4+ T cells and disease progression in HIV-1 perinatally infected children
    Elizabeth R Sharp
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 7:e29154. 2012
    ..In the USA, most HIV-1 infected children are on antiretroviral drug regimens, with many individuals surviving through adolescence and into adulthood. The course of HIV-1 infection in these children is variable, and understudied...
  5. pmc Immunodominance of HIV-1 specific CD8+ T-cell responses is related to disease progression rate in vertically infected adolescents
    Elizabeth R Sharp
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 6:e21135. 2011
    ..We hypothesized that differences in the pattern of immunodomination including breadth, magnitude and polyfunctionality of HIV-1 specific CD8+ T cell response could partially explain differences in progression rate...
  6. pmc Increased expression of intrinsic antiviral genes in HLA-B*57-positive individuals
    Rui André Saraiva Raposo
    2 Division of Experimental Medicine, 1001 Potrero Ave, Bldg 3, Room 603, Box 1234, San Francisco, CA 94110, USA
    J Leukoc Biol 94:1051-9. 2013
    ..Our results provide insights into natural protection mechanisms and immunity against HIV-1 that fall outside of classical HLA-mediated effects. ..
  7. pmc Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques
    Karin J Metzner
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, NY 10016, USA
    Retrovirology 3:97. 2006
    ....
  8. pmc The frequency of CD127low expressing CD4+CD25high T regulatory cells is inversely correlated with human T lymphotrophic virus type-1 (HTLV-1) proviral load in HTLV-1-infection and HTLV-1-associated myelopathy/tropical spastic paraparesis
    Jakob Michaelsson
    Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA
    BMC Immunol 9:41. 2008
    ..The purpose of this study was to assess the frequency and phenotype of TReg cells in HTLV-1 asymptomatic carriers and in HTLV-1-associated neurological disease (HAM/TSP) patients, and to correlate with measures of T cell activation...
  9. pmc T cell responses to human endogenous retroviruses in HIV-1 infection
    Keith E Garrison
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS Pathog 3:e165. 2007
    ..HERV-specific immunity is an important new avenue for investigation in HIV-1 pathogenesis and vaccine design...
  10. ncbi request reprint Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells
    Annika C Karlsson
    Gladstone Institute of Virology and Immunology, University of California San Francisco, P O Box 419100, San Francisco, CA 94141 9100, USA
    J Immunol Methods 283:141-53. 2003
    ..In contrast, the CFC has a greater dynamic range and allows for phenotypic discrimination of responding cells, making it the assay of choice for most other applications...
  11. ncbi request reprint FOXP3 expressing CD127lo CD4+ T cells inversely correlate with CD38+ CD8+ T cell activation levels in primary HIV-1 infection
    Lishomwa C Ndhlovu
    Division of Experimental Medicine, San Francisco General Hospital, University of California San Francisco, San Francisco, CA 94110, USA
    J Leukoc Biol 83:254-62. 2008
    ....
  12. pmc R5 human immunodeficiency virus type 1 (HIV-1) replicates more efficiently in primary CD4+ T-cell cultures than X4 HIV-1
    Becky Schweighardt
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141 9100, USA
    J Virol 78:9164-73. 2004
    ..In summary, these data demonstrate that R5 HIV-1 has an increased capacity to replicate in costimulated CD4+ T cells compared to X4 HIV-1...
  13. ncbi request reprint Partial treatment interruption of protease inhibitors augments HIV-specific immune responses in vertically infected pediatric patients
    Fatema A Legrand
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94158 2261, USA
    AIDS 19:1575-85. 2005
    ..Protease inhibitor (PI) based therapy has been hypothesized to depress cell-mediated immune responses by reducing antigen presentation...
  14. ncbi request reprint Higher frequency of HIV-1-specific T cell immune responses in African American children vertically infected with HIV-1
    Elizabeth R Sharp
    Gladstone Institute of Virology and Immunology, San Francisco General Hospital, University of California, 94158, USA
    J Infect Dis 192:1772-80. 2005
    ..01) than Hispanic children; this was significantly driven by robust responses in African American girls near the age of puberty, many of whom carried the human leukocyte antigen class I B*58 allele...
  15. ncbi request reprint Seroreversion in subjects receiving antiretroviral therapy during acute/early HIV infection
    C Bradley Hare
    Positive Health Program, University of California, San Francisco, San Francisco, CA, USA
    Clin Infect Dis 42:700-8. 2006
    ....
  16. pmc Residual viral replication during antiretroviral therapy boosts human immunodeficiency virus type 1-specific CD8+ T-cell responses in subjects treated early after infection
    Gabriel M Ortiz
    Aaron Diamond AIDS Research Center, The Rockefeller University, New York, New York 10016, USA
    J Virol 76:411-5. 2002
    ..HIV-1-specific CD8+ T cells can increase in number after HAART in subjects treated early after infection who have episodes of transient viremia...
  17. pmc Suppression of HIV-1 plasma viral load below detection preserves IL-17 producing T cells in HIV-1 infection
    Lishomwa C Ndhlovu
    Division of Experimental Medicine, San Francisco General Hospital, Department of Medicine, University of California, San Francisco, California 94110, USA
    AIDS 22:990-2. 2008
    ..These results imply viral-mediated destruction or impairment of Th17 cells and argue for complete suppression of viremia for reconstitution of Th17 cells...
  18. ncbi request reprint CD7 is a differentiation marker that identifies multiple CD8 T cell effector subsets
    Einar M Aandahl
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94141, USA
    J Immunol 170:2349-55. 2003
    ..These data provide a model of human CD8 T cell differentiation in which specialized distinct subpopulations can be identified by expression of CD7...
  19. pmc Dual pressure from antiretroviral therapy and cell-mediated immune response on the human immunodeficiency virus type 1 protease gene
    Annika C Karlsson
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141, USA
    J Virol 77:6743-52. 2003
    ..Our results indicate that both HIV type 1-specific CD8(+) T cells and antiretroviral drugs provide complex pressures on the same amino acid sequence of the HIV protease gene and, thus, can influence viral sequence evolution...
  20. ncbi request reprint Inhibition of antigen-specific T cell proliferation and cytokine production by protein kinase A type I
    Einar Martin Aandahl
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94141, USA
    J Immunol 169:802-8. 2002
    ..This supports the notion of PKA type I as a key enzyme in the negative regulation of immune responses and a potential target for inhibiting autoreactive T cells...
  21. ncbi request reprint Dendritic cell amplification of HIV type 1-specific CD8+ T cell responses in exposed, seronegative heterosexual women
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, PO Box 419100, San Francisco, CA 94110 9100, USA
    AIDS Res Hum Retroviruses 18:805-15. 2002
    ..This report also demonstrates the ability of dendritic cells to reveal T cell responses that might be overlooked by other methods...
  22. pmc IL-2 immunotherapy to recently HIV-1 infected adults maintains the numbers of IL-17 expressing CD4+ T (T(H)17) cells in the periphery
    Lishomwa C Ndhlovu
    Division of Experimental Medicine, Department of Medicine, San Francisco General Hospital, University of California, San Francisco, San Francisco, CA, USA
    J Clin Immunol 30:681-92. 2010
    ..However, IL-2-mediated T-reg expansions may selectively reduce responses to certain antigen-specific populations, such as HIV-1 Gag...
  23. doi request reprint Lower cytokine secretion ex vivo by natural killer T cells in HIV-infected individuals is associated with higher CD161 expression
    Jennifer E Snyder-Cappione
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    AIDS 23:1965-70. 2009
    ..This study measured NKT cell cytokine production directly ex vivo and compared these responses with both the disease status and NKT subset distribution of individual patients...
  24. pmc Trafficking of human immunodeficiency virus type 1-specific CD8+ T cells to gut-associated lymphoid tissue during chronic infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141 9100, USA
    J Virol 77:5621-31. 2003
    ..These studies demonstrate the feasibility of using tetramers to assess HIV-specific T cells in GALT and reveal that GALT is the site of an active CD8(+) T-cell response during chronic infection...
  25. pmc A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties
    Lishomwa C Ndhlovu
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, and HIV AIDS Division, San Francisco General Hospital, San Francisco, CA 94110, USA
    Eur J Immunol 40:134-41. 2010
    ..We propose that this novel CD4+ T-cell population counterbalances the suppressive activity of suppressor Treg in peripheral blood and serves as a calibrator of immunoregulation...
  26. pmc Identification of human endogenous retrovirus-specific T cell responses in vertically HIV-1-infected subjects
    Ravi Tandon
    Division of Experimental Medicine, University of California San Francisco, Department of Medicine, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    J Virol 85:11526-31. 2011
    ..These data indicate that HERV-specific T cells may participate in controlling HIV-1 replication and that certain highly conserved HERV-derived proteins may serve as promising therapeutic vaccine targets in HIV-1-infected children...
  27. pmc Human immunodeficiency virus type 1 (HIV-1)-specific CD8+ T(EMRA) cells in early infection are linked to control of HIV-1 viremia and predict the subsequent viral load set point
    John W Northfield
    Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, South Parks Road, Oxford OX1 3SY, United Kingdom
    J Virol 81:5759-65. 2007
    ....
  28. pmc Cutting Edge: KIR3DS1, a gene implicated in resistance to progression to AIDS, encodes a DAP12-associated receptor expressed on NK cells that triggers NK cell activation
    William H Carr
    Department of Microbiology and Immunology, Cancer Research Institute and Biomedical Sciences Graduate Program, University of California San Francisco, CA 94143, USA
    J Immunol 178:647-51. 2007
    ..Soluble KIR3DS1-Ig fusion proteins did not bind to EBV-transformed B lymphoid cell lines transfected with HLA-Bw4 80I or 80T allotypes, suggesting that if KIR3DS1 does recognize HLA-Bw4 ligands, this may be peptide dependent...
  29. ncbi request reprint Natural killer cells in perinatally HIV-1-infected children exhibit less degranulation compared to HIV-1-exposed uninfected children and their expression of KIR2DL3, NKG2C, and NKp46 correlates with disease severity
    Wassim M Ballan
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, USA
    J Immunol 179:3362-70. 2007
    ..This finding suggests that NK cells may have an important role in HIV-1 disease pathogenesis in HIV-1-infected children...
  30. pmc HIV-1/HSV-2 co-infected adults in early HIV-1 infection have elevated CD4+ T cell counts
    Jason D Barbour
    HIV AIDS Division, San Francisco General Hospital, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 2:e1080. 2007
    ..We examined the impact of HSV-2 status at the time of HIV-1 acquisition for its impact on subsequent clinical course, and total CD4+ T cell phenotypes...
  31. doi request reprint Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: Panel design by an empiric approach
    Bridget E McLaughlin
    Division of Infectious Diseases, Internal Medicine, University of California Davis, Davis, CA, USA
    Cytometry A 73:400-10. 2008
    ..Careful reagent titration and testing of multiple candidate panels are necessary to ensure quality results in multiparametric measurements...
  32. ncbi request reprint Changes in CD4+ T-cell differentiation phenotype during structured treatment interruption in patients with chronic HIV-1 infection
    Thomas H Alexander
    Duke University Medical Center, Durham, NC 27705, USA
    J Acquir Immune Defic Syndr 34:475-81. 2003
    ..These results suggest that interruptions should be avoided whenever possible in patients on HAART with high plasma HIV-1 RNA set-points...
  33. pmc Immune reconstitution of CD56(dim) NK cells in individuals with primary HIV-1 infection treated with interleukin-2
    Jakob Michaelsson
    Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Stockholm, Sweden
    J Infect Dis 197:117-25. 2008
    ..Importantly, NK cell receptor expression and IFN-gamma production were maintained over time. This reconstitution of NK cells may be useful in helping contain viremia if patients discontinue therapy or develop drug resistance...
  34. pmc Single-cell, phosphoepitope-specific analysis demonstrates cell type- and pathway-specific dysregulation of Jak/STAT and MAPK signaling associated with in vivo human immunodeficiency virus type 1 infection
    Andrew W Lee
    Division of Experimental Medicine, University of California at San Francisco, 1001 Potrero Ave, Bldg 3, UCSF Box 1234, San Francisco, CA 94143 1234, USA
    J Virol 82:3702-12. 2008
    ..This understanding of altered monocyte signaling responses that contribute to defective antigen presentation during HIV-1 infection could lead to immunotherapeutic approaches that compensate for the deficiency...
  35. pmc Conferral of enhanced natural killer cell function by KIR3DS1 in early human immunodeficiency virus type 1 infection
    Brian R Long
    Division of Experimental Medicine, Department of Medicine, San Francisco General Hospital, University of California, San Francisco, USA
    J Virol 82:4785-92. 2008
    ....
  36. ncbi request reprint Broadly immunogenic HLA class I supertype-restricted elite CTL epitopes recognized in a diverse population infected with different HIV-1 subtypes
    Carina L Pérez
    Department of Microbiology, Cell Biology, and Tumor Biology, Karolinska Institutet, and The Swedish Institute of Infectious Disease Control, Stockholm, Sweden
    J Immunol 180:5092-100. 2008
    ..Our results have important implications for the validation of potent CTL responses and show that the goal for a vaccine candidate in inducing broadly reactive CTL immune responses is attainable...
  37. doi request reprint Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part II: Panel performance across different instrument platforms
    Bridget E McLaughlin
    University of California Davis, Internal Medicine, Infectious Diseases, Davis, CA, USA
    Cytometry A 73:411-20. 2008
    ....
  38. ncbi request reprint HIV-1-specific CD4+ T cell responses in chronically HIV-1 infected blippers on antiretroviral therapy in relation to viral replication following treatment interruption
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Clin Immunol 26:40-54. 2006
    ..04) with no difference between groups by week 24. These data support that blippers have higher anti-HIV lymphoproliferative responses while on ART but experience equal to higher viral rebound as compared to matched non-blippers upon TI...
  39. ncbi request reprint CD8 T cell effector maturation in HIV-1-infected children
    Kimberly A Jordan
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94158, USA
    Virology 347:117-26. 2006
    ..The data also suggest, however, that the perforin-deficient state of HIV-specific CD8 T cells in children may be reversible...
  40. pmc Expansion of CD1d-restricted NKT cells in patients with primary HIV-1 infection treated with interleukin-2
    Markus Moll
    CIM, Department of Medicine, F59, Karolinska Institute, Karolinska University Hospital, Huddinge, 14186 Stockholm, Sweden
    Blood 107:3081-3. 2006
    ..These data indicate that IL-2 treatment in combination with effective ART is beneficial for the restoration of innate NKT cell immunity in patients with primary HIV-1 infection...
  41. pmc Highly effective control of an AIDS virus challenge in macaques by using vesicular stomatitis virus and modified vaccinia virus Ankara vaccine vectors in a single-boost protocol
    Elizabeth Ramsburg
    Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Virol 78:3930-40. 2004
    ..The improved protection in MVA-boosted animals correlates with trends toward stronger prechallenge CD8(+)-T-cell responses to SHIV antigens and stronger postchallenge SHIV-neutralizing antibody production...
  42. pmc Human CD4+ CD25+ regulatory T cells control T-cell responses to human immunodeficiency virus and cytomegalovirus antigens
    Einar M Aandahl
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, California 94110, USA
    J Virol 78:2454-9. 2004
    ..We propose that chronic viral infections lead to induction of suppressive T(R) cells that inhibit the antiviral immune response...
  43. ncbi request reprint Poorly soluble peptides can mimic authentic ELISPOT responses
    R Karl Karlsson
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94141, USA
    J Immunol Methods 285:89-92. 2004
    ..Filtering the peptide prevented non-specific spot formation. These findings underscore the need for appropriate controls and proper peptide preparation in order to reduce the risk of false-positive ELISPOT responses...
  44. ncbi request reprint Optimization of methods to assess human mucosal T-cell responses to HIV infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA, USA
    J Immunol Methods 279:17-31. 2003
    ..Taken together, these methods will be useful for studies of mucosal immunity to HIV-1 and other pathogens during natural infection and following vaccination...
  45. ncbi request reprint Presence of human immunodeficiency virus-1-specific CD4 and CD8 cellular immune responses in children with full or partial virus suppression
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, USA
    J Infect Dis 188:873-82. 2003
    ..Overall, a history of stable CD4(+) T cell counts, as a result of therapy that imparted full or partial virus suppression, was associated with increased levels of anti-HIV CD4(+) T helper responses and decreased T cell activation...
  46. ncbi request reprint HIV-specific CD8+ T cell function in children with vertically acquired HIV-1 infection is critically influenced by age and the state of the CD4+ T cell compartment
    Johan K Sandberg
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94141, USA
    J Immunol 170:4403-10. 2003
    ..The results are discussed in relation to differences between the neonatal and adult immune systems in the ability to respond to HIV infection...
  47. ncbi request reprint Dominant effector memory characteristics, capacity for dynamic adaptive expansion, and sex bias in the innate Valpha24 NKT cell compartment
    Johan K Sandberg
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA, USA
    Eur J Immunol 33:588-96. 2003
    ..Thus, the two NKT cell subsets display different properties and dynamics that will influence their function as innate immunoregulatory and effector cells...
  48. ncbi request reprint Emergence of drug-resistant HIV-1 variants in patients undergoing structured treatment interruptions
    Becky Schweighardt
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA, USA
    AIDS 16:2342-4. 2002
    ..We concluded that the repeated abrupt termination and resumption of certain antiretroviral drug regimens during STI therapy may lead to the development of drug resistance in chronically HIV-infected individuals...
  49. ncbi request reprint Enhanced ELISPOT detection of antigen-specific T cell responses from cryopreserved specimens with addition of both IL-7 and IL-15--the Amplispot assay
    Wim Jennes
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, CA, USA
    J Immunol Methods 270:99-108. 2002
    ..This method will be particularly useful for the assessment of antigen-stimulated cytokine production by T cells in cryopreserved biological specimens...
  50. ncbi request reprint Generation of CD3+CD8low thymocytes in the HIV type 1-infected thymus
    Mary E Keir
    Biomedical Sciences Graduate Program, University of California, San Francisco 94143, USA
    J Immunol 169:2788-96. 2002
    ..We suggest that this HIV-1-initiated selection process may contribute to the generation of dysfunctional CD8(+) T cells in HIV-1-infected patients...
  51. ncbi request reprint T lymphocyte responses against human parvovirus B19: small virus, big response
    Paul Klenerman
    Nuffield Dept of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK
    Pathol Biol (Paris) 50:317-25. 2002
    ....
  52. pmc Selective loss of innate CD4(+) V alpha 24 natural killer T cells in human immunodeficiency virus infection
    Johan K Sandberg
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141, USA
    J Virol 76:7528-34. 2002
    ..These data indicate that the key immunoregulatory NKT-cell compartment is compromised in HIV-1-infected patients...
  53. pmc Correlates of nontransmission in US women at high risk of human immunodeficiency virus type 1 infection through sexual exposure
    Joan H Skurnick
    Department of Preventive Medicine and Community Health, New Jersey Medical School, Newark, NJ 07103 2714, USA
    J Infect Dis 185:428-38. 2002
    ..These observations suggest that the primary protective factor is CD8 cell activity in both the HIV-positive donor and the HIV-negative partner. These findings have substantial implications for vaccine development...
  54. ncbi request reprint Amplification of low-frequency antiviral CD8 T cell responses using autologous dendritic cells
    Marie Larsson
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY, USA
    AIDS 16:171-80. 2002
    ..To utilize the potent antigen-presenting capacity of mature dendritic cells (MDC) in order to develop a rapid, sensitive method for quantifying antigen-specific CD8 T cells present at low frequency in peripheral blood...
  55. ncbi request reprint New observations on CD8 cell responses
    Douglas F Nixon
    Gladstone Institute of Virology and Immunology, PO Box 419100, San Francisco, CA 94141 9100, USA
    AIDS 17:S61-5. 2003
  56. ncbi request reprint Immunologic and virologic evolution during periods of intermittent and persistent low-level viremia
    Annika C Karlsson
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, USA
    AIDS 18:981-9. 2004
    ..The interaction of these factors is not well understood, particularly in the setting of antiretroviral therapy...
  57. ncbi request reprint Multidrug-resistant, dual-tropic HIV-1 and rapid progression
    Douglas F Nixon
    Lancet 365:1924-5. 2005
  58. ncbi request reprint Expansion of CD7(low) and CD7(negative) CD8 T-cell effector subsets in HIV-1 infection: correlation with antigenic load and reversion by antiretroviral treatment
    Einar M Aandahl
    The Biotechnology Centre of Oslo, University of Oslo, Norway
    Blood 104:3672-8. 2004
    ..The results are discussed in relation to the dual roles of CD7 as a receptor of both costimulation and cell death...
  59. ncbi request reprint Relevance of HIV-1-specific CD4+ helper T-cell responses during structured treatment interruptions in patients with CD4+ T-cell nadir above 400/mm3
    Montserrat Plana
    Clinic Institute of Infectious Diseases and Immunology, IDIBAPS, Hospital Clinic, Faculty of Medicine, University of Barcelona, Spain
    J Acquir Immune Defic Syndr 36:791-9. 2004
    ..To analyze the dynamics of both HIV-1-specific CD4 and CD8 T-cell responses during structured treatment interruptions (STIs) in chronically HIV-1-infected (CHI) patients and to correlate them with the viral set point achieved...
  60. ncbi request reprint Evaluation of CD8+ T-cell and antibody responses following transient increased viraemia in rhesus macaques infected with live, attenuated simian immunodeficiency virus
    Karin J Metzner
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, NY 10016, USA
    J Gen Virol 86:3375-84. 2005
    ....
  61. ncbi request reprint Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: implications for immune control of HIV-1 infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology GIVI, Department of Medicine, University of California San Francisco, San Francisco, CA 94141, USA
    J Immunol 173:641-8. 2004
    ..Furthermore, the relative absence of armed effector cells may serve to protect the integrity of rectal mucosa under normal conditions, but might also provide an early advantage to HIV-1 and other sexually transmitted viruses...
  62. ncbi request reprint Increased adhesion molecule and chemokine receptor expression on CD8+ T cells trafficking to cerebrospinal fluid in HIV-1 infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California, USA
    J Infect Dis 189:2202-12. 2004
    ..The central nervous system (CNS) is a recognized target for human immunodeficiency virus type 1 (HIV-1). CD8(+) T cells may mediate viral clearance from the CNS but also may contribute to immune-mediated neuronal damage...
  63. pmc Phenotypic, functional, and kinetic parameters associated with apparent T-cell control of human immunodeficiency virus replication in individuals with and without antiretroviral treatment
    Brinda Emu
    Department of Medicine, San Francisco General Hospital, University of California, 94110, USA
    J Virol 79:14169-78. 2005
    ....
  64. ncbi request reprint Regulation of T cell responses in the developing human fetus
    Jakob Michaelsson
    Gladstone Institute of Virology and Immunology, University of California San Francisco, 1001 Potrero Avenue, San Francisco, CA 94158, USA
    J Immunol 176:5741-8. 2006
    ....
  65. ncbi request reprint A plea for justice for jailed medical workers
    Sunil K Ahuja
    Science 314:924-5. 2006
  66. ncbi request reprint Detection of T lymphocytes specific for human endogenous retrovirus K (HERV-K) in patients with seminoma
    Seth Rakoff-Nahoum
    J D Gladstone Institutes, Gladstone Institute of Virology and Immunology, San Francisco, California 94158, USA
    AIDS Res Hum Retroviruses 22:52-6. 2006
    ..In summary, we detected T cell reactivity to HERV-K peptides in both past seminoma patients and a minority of apparently healthy controls...
  67. ncbi request reprint ELISPOT cell rescue
    Duncan A Meiklejohn
    Gladstone Institute of Virology and Immunology, University of California, PO Box 419100, San Francisco, CA 94141 9100, USA
    J Immunol Methods 288:135-47. 2004
    ..Rescue of cells after an ELISPOT assay will be particularly useful for increasing sample utility and maximizing data collection from T cell assays in vaccine trials...
  68. ncbi request reprint CD4+CD25+ regulatory T cells in HIV infection
    Douglas F Nixon
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco, CA, USA
    Microbes Infect 7:1063-5. 2005
    ..In this article we review the current knowledge and potential role of CD4(+)CD25(+) T(Reg) cells in HIV infection...
  69. ncbi request reprint Individuals with pulmonary tuberculosis have lower levels of circulating CD1d-restricted NKT cells
    Jennifer E Snyder-Cappione
    Division of Experimental Medicine, University of California, San Francisco, CA 94143, USA
    J Infect Dis 195:1361-4. 2007
    ..This apparent loss of NKT cells from the peripheral blood is sustained during the 6 months after the initiation of MTB treatment. These findings indicate that NKT cells may be an important component of antituberculosis immunity...
  70. ncbi request reprint Boosting of SIV-specific T cell responses in rhesus macaques that resist repeated intravaginal challenge with SIVmac251
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, California 94103, USA
    AIDS Res Hum Retroviruses 18:1081-8. 2002
    ..These results support a protective role for SIVmac-specific T cells in repeatedly exposed, persistently seronegative rhesus macaques...
  71. pmc Development of innate CD4+ alpha-chain variable gene segment 24 (Valpha24) natural killer T cells in the early human fetal thymus is regulated by IL-7
    Johan K Sandberg
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94103, USA
    Proc Natl Acad Sci U S A 101:7058-63. 2004
    ..Furthermore, data suggest an intrathymic pathway of CD4+ Valpha24 NK T cell development that is regulated by IL-7...
  72. pmc Elevated frequency of gamma interferon-producing NK cells in healthy adults vaccinated against influenza virus
    Brian R Long
    Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, San Francisco, California 941101, USA
    Clin Vaccine Immunol 15:120-30. 2008
    ..These results may form the basis for further investigations of the role of NK cells in immunity to influenza...

Research Grants32

  1. DYNAMICS OF HIV1 SPECIFIC CTL IN HIV1 INFECTION
    Douglas Nixon; Fiscal Year: 2001
    ..These studies of CTL dynamics will likely contribute considerably to understanding the role of HIV-1 specific CTL and vaccine strategies designed to induce CTL in the context of a prophylactic anti-HIV-1 vaccine. ..
  2. Viral Tropism and Immune Control in HIV
    Douglas Nixon; Fiscal Year: 2006
    ..If X4 virus is preferentially affected by CD8+ T cells, we will then assess whether this preferential control occurs because of the virus itself, or because of the targets infected. ..
  3. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2005
    ..We believe that the proposed studies will contribute considerably to our understanding of NKT cell function in HIV disease, how the virus affects them, and how they may contribute to innate defense against HIV...
  4. T Regulatory Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2005
    ..The goal of this proposal is to generate data to determine the importance of T=g cells in HIV infection. Manipulation of T=g cells could be critical for vaccine strategies and for therapeutic interventions. ..
  5. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2004
    ..We believe that the proposed studies will contribute considerably to our understanding of NKT cell function in HIV disease, how the virus affects them, and how they may contribute to innate defense against HIV...
  6. T Regulatory Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2006
    ..The goal of this proposal is to generate data to determine the importance of Treg cells in HIV infection. Manipulation of Treg cells could be critical for vaccine strategies and for therapeutic interventions. ..
  7. T Regulatory Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2009
    ..The goal of this proposal is to generate data to determine the importance of Treg cells in HIV infection. Manipulation of Treg cells could be critical for vaccine strategies and for therapeutic interventions. ..
  8. DYNAMICS OF HIV1 SPECIFIC CTL IN HIV1 INFECTION
    Douglas Nixon; Fiscal Year: 2003
    ..These studies of CTL dynamics will likely contribute considerably to understanding the role of HIV-1 specific CTL and vaccine strategies designed to induce CTL in the context of a prophylactic anti-HIV-1 vaccine. ..
  9. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2002
    ..We believe that the proposed studies will contribute considerably to our understanding of NKT cell function in HIV disease, how the virus affects them, and how they may contribute to innate defense against HIV...
  10. HIV VARIATION AND CROSS CLADE CTL RECOGNITION
    Douglas Nixon; Fiscal Year: 2002
    ..In the second specific aim, we will ascertain whether infection with HlV~2 stimulates CTL which cross react with HIV-1 or vice versa, and whether some individuals are protected by one clade of HIV-1 against super-infection with another. ..
  11. Human Endogenous Retroelements in HIV Immunopathogenesis
    Douglas Nixon; Fiscal Year: 2009
    ..This information will be used to devise future studies to treat HIV infection and design a novel vaccine against HIV/AIDS. ..
  12. DYNAMICS OF HIV1 SPECIFIC CTL IN HIV1 INFECTION
    Douglas Nixon; Fiscal Year: 2000
    ..These studies of CTL dynamics will likely contribute considerably to understanding the role of HIV-1 specific CTL and vaccine strategies designed to induce CTL in the context of a prophylactic anti-HIV-1 vaccine. ..
  13. Human Endogenous Retroelements in HIV Immunopathogenesis
    Douglas F Nixon; Fiscal Year: 2010
    ..This information will be used to devise future studies to treat HIV infection and design a novel vaccine against HIV/AIDS. ..
  14. Cell Mediated Immunity in HIV Infected Children
    Douglas Nixon; Fiscal Year: 2009
    ..The goal of this grant application is to better understand immunopathogenic factors relevant to HIV infected children so they can be better placed to take advantage of developments in vaccines and new treatments. ..
  15. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2006
    ..We believe that the proposed studies will contribute considerably to our understanding of NKT cell function in HIV disease, how the virus affects them, and how they may contribute to innate defense against HIV...
  16. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2007
    ....
  17. HIV VARIATION AND CROSS CLADE CTL RECOGNITION
    Douglas Nixon; Fiscal Year: 2001
    ..In the second specific aim, we will ascertain whether infection with HlV~2 stimulates CTL which cross react with HIV-1 or vice versa, and whether some individuals are protected by one clade of HIV-1 against super-infection with another. ..
  18. HIV VARIATION AND CROSS CLADE CTL RECOGNITION
    Douglas Nixon; Fiscal Year: 2000
    ..In the second specific aim, we will ascertain whether infection with HlV~2 stimulates CTL which cross react with HIV-1 or vice versa, and whether some individuals are protected by one clade of HIV-1 against super-infection with another. ..
  19. DYNAMICS OF HIV1 SPECIFIC CTL IN HIV1 INFECTION
    Douglas Nixon; Fiscal Year: 2002
    ..These studies of CTL dynamics will likely contribute considerably to understanding the role of HIV-1 specific CTL and vaccine strategies designed to induce CTL in the context of a prophylactic anti-HIV-1 vaccine. ..
  20. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2003
    ..We believe that the proposed studies will contribute considerably to our understanding of NKT cell function in HIV disease, how the virus affects them, and how they may contribute to innate defense against HIV...
  21. HIV VARIATION AND CROSS CLADE CTL RECOGNITION
    Douglas Nixon; Fiscal Year: 2003
    ..In the second specific aim, we will ascertain whether infection with HlV~2 stimulates CTL which cross react with HIV-1 or vice versa, and whether some individuals are protected by one clade of HIV-1 against super-infection with another. ..
  22. Cell Mediated Immunity in HIV Infected Children
    Douglas Nixon; Fiscal Year: 2005
    ..The goal of this grant application is to better understand immunopathogenic factors relevant to HIV infected children so they can be better placed to take advantage of developments in vaccines and new treatments. ..
  23. Cell Mediated Immunity in HIV Infected Children
    Douglas Nixon; Fiscal Year: 2006
    ..The goal of this grant application is to better understand immunopathogenic factors relevant to HIV infected children so they can be better placed to take advantage of developments in vaccines and new treatments. ..
  24. Innate NKT Cells in HIV Infection
    Douglas Nixon; Fiscal Year: 2006
    ..We believe that the proposed studies will contribute considerably to our understanding of NKT cell function in HIV disease, how the virus affects them, and how they may contribute to innate defense against HIV...
  25. Cell Mediated Immunity in HIV Infected Children
    Douglas Nixon; Fiscal Year: 2007
    ..The goal of this grant application is to better understand immunopathogenic factors relevant to HIV infected children so they can be better placed to take advantage of developments in vaccines and new treatments. ..
  26. Viral Tropism and Immune Control in HIV
    Douglas Nixon; Fiscal Year: 2007
    ..If X4 virus is preferentially affected by CD8+ T cells, we will then assess whether this preferential control occurs because of the virus itself, or because of the targets infected. ..
  27. T Regulatory Cells in HIV Infection
    Douglas F Nixon; Fiscal Year: 2010
    ..The goal of this proposal is to generate data to determine the importance of Treg cells in HIV infection. Manipulation of Treg cells could be critical for vaccine strategies and for therapeutic interventions. ..