Research Topics
Genomes and Genes | Michael NiederweisSummaryAffiliation: University of Alabama at Birmingham Country: USA Publications
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Publications
Purification of porins from Mycobacterium smegmatisChristian Heinz
, , Germany
Methods Mol Biol 228:139-50. 2003
Identification of two Mycobacterium smegmatis lipoproteins exported by a SecA2-dependent pathwayHenry S Gibbons
Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7290, USA
J Bacteriol 189:5090-100. 2007..smegmatis and M. tuberculosis...- Functional expression of the Flp recombinase in Mycobacterium bovis BCGHouhui Song
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
Gene 399:112-9. 2007..smegmatis, but can also be used in slow growing mycobacteria such as M. tuberculosis for constructing unmarked mutations. Other more sophisticated applications in mycobacterial genetics would also profit from the improved Flp/FRT system... - Mycobacterial porins--new channel proteins in unique outer membranesMichael Niederweis
Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstr 5, 91058 Erlangen, Germany
Mol Microbiol 49:1167-77. 2003..smegmatis for hydrophilic solutes. The importance of the synergism between slow transport through the porins and drug efflux or inactivation for the development of drugs against M. tuberculosis is discussed... - Porins are required for uptake of phosphates by Mycobacterium smegmatisFrank Wolschendorf
Department of Microbiology, University of Alabama at Birmingham, Mail Box 24, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
J Bacteriol 189:2435-42. 2007..However, porins that could mediate the diffusion of phosphates across the OM of M. bovis BCG and Mycobacterium tuberculosis are unknown... - DNA-free RNA preparations from mycobacteriaJoachim Stephan
Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstr, 5, D 91058 Erlangen, Germany
BMC Microbiol 4:45. 2004..To understand mycobacterial pathogenesis analysis of gene expression by quantification of RNA levels becomes increasingly important. However, current preparation methods yield mycobacterial RNA that is contaminated with chromosomal DNA... - Mycobacterial outer membranes: in search of proteinsMichael Niederweis
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
Trends Microbiol 18:109-16. 2010..Through comparison to transport processes in Gram-negative bacteria, we highlight several hypothetical outer membrane proteins of M. tuberculosis that await discovery... 
Nutrient acquisition by mycobacteriaMichael Niederweis
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
Microbiology 154:679-92. 2008..tuberculosis will not only promote our understanding of the physiology of this important human pathogen, but might also be exploited to improve tuberculosis chemotherapy...- Rv1698 of Mycobacterium tuberculosis represents a new class of channel-forming outer membrane proteinsAxel Siroy
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
J Biol Chem 283:17827-37. 2008..tuberculosis. Rv1698 has single homologs of unknown functions in Corynebacterineae and thus represents the first member of a new class of channel proteins specific for mycolic acid-containing outer membranes... - Identification of a novel multidrug efflux pump of Mycobacterium tuberculosisOlga Danilchanka
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, Alabama 35294, USA
Antimicrob Agents Chemother 52:2503-11. 2008..Both effects were abrogated in the presence of the efflux pump inhibitor reserpine. These results demonstrate that Rv0194 is a novel multidrug efflux pump of M. tuberculosis... - Construction of unmarked deletion mutants in mycobacteriaHouhui Song
Department of Microbiology, University of Alabama at Birmingham, Alabama 35294, USA
Methods Mol Biol 465:279-95. 2009..In this chapter, we describe strategies and methods of how to use sequence-specific recombination mediated by Flp and Cre to construct mutants of Mycobacterium smegmatis, Mycobacterium bovis BCG, and Mycobacterium tuberculosis... - Functions of the periplasmic loop of the porin MspA from Mycobacterium smegmatisJason Huff
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J Biol Chem 284:10223-31. 2009..This is the first study identifying a molecular determinant of solute translocation in a mycobacterial porin... - Role of porins for uptake of antibiotics by Mycobacterium smegmatisOlga Danilchanka
Department of Microbiology, University of Alabama at Birmingham, Alabama 35294, USA
Antimicrob Agents Chemother 52:3127-34. 2008..avium, M. chelonae, and M. fortuitum, which have Msp-like porins, to acquire resistance to fluoroquinolones... - Expression of the ompATb operon accelerates ammonia secretion and adaptation of Mycobacterium tuberculosis to acidic environmentsHouhui Song
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
Mol Microbiol 80:900-18. 2011..Taken together, these results show that the ompATb operon is necessary for rapid ammonia secretion and adaptation of M. tuberculosis to acidic environments in vitro but not in mice... - Uptake of sulfate but not phosphate by Mycobacterium tuberculosis is slower than that for Mycobacterium smegmatisHouhui Song
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA
J Bacteriol 194:956-64. 2012..tuberculosis. However, the uptake of these anions by M. tuberculosis is orders of magnitude faster than diffusion through lipid membranes, indicating that unknown outer membrane proteins are required to facilitate this process... - Copper resistance is essential for virulence of Mycobacterium tuberculosisFrank Wolschendorf
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Proc Natl Acad Sci U S A 108:1621-6. 2011..Hence, this study reveals an Achilles heel of Mtb that might be a promising target for tuberculosis chemotherapy... - Identification of outer membrane proteins of Mycobacterium tuberculosisHouhui Song
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
Tuberculosis (Edinb) 88:526-44. 2008..tuberculosis likely has many OMPs with beta-barrel structure. Our findings pave the way to identify the set of proteins which functionalize the outer membrane of M. tuberculosis... - Mycobacterium tuberculosis can utilize heme as an iron sourceChristopher M Jones
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
J Bacteriol 193:1767-70. 2011..Here, we demonstrate that Mycobacterium tuberculosis can utilize heme as an iron source, suggesting that M. tuberculosis possesses a yet-unknown heme acquisition system... - Antimycobacterial activity in vitro of pigments isolated from Antarctic bacteriaNazia Mojib
Department of Biology, University of Alabama at Birmingham, 35294 1170, USA
Antonie Van Leeuwenhoek 98:531-40. 2010..Our results indicate these pigments isolated from Antarctic bacteria might be valuable lead compounds for new antimycobacterial drugs used for chemotherapy of tuberculosis... - Copper-boosting compounds: a novel concept for antimycobacterial drug discoveryAlexander Speer
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA
Antimicrob Agents Chemother 57:1089-91. 2013..tuberculosis and demonstrate that such copper-boosting compounds are effective against replicating and nonreplicating M. tuberculosis strains... - Role of porins in the susceptibility of Mycobacterium smegmatis and Mycobacterium chelonae to aldehyde-based disinfectants and drugsZuzana Svetlikova
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 1682, USA
Antimicrob Agents Chemother 53:4015-8. 2009..chelonae to drugs, there is thus some concern that the widespread use of glutaraldehyde and ortho-phthalaldehyde in clinical settings may select for drug-resistant bacteria... - Role of porins in iron uptake by Mycobacterium smegmatisChristopher M Jones
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, Alabama 35294, USA
J Bacteriol 192:6411-7. 2010..These results provide, to our knowledge, the first experimental evidence that general porins are indeed the outer membrane conduit of low-affinity iron acquisition systems in bacteria... - Resistance mechanisms of Mycobacterium tuberculosis against phagosomal copper overloadJennifer L Rowland
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
Tuberculosis (Edinb) 92:202-10. 2012..These findings reveal an intricate interplay between the host which aims to overload the phagosome with copper and M. tuberculosis which utilizes several mechanisms to reduce the toxic effects of excess copper... - Discovery of a siderophore export system essential for virulence of Mycobacterium tuberculosisRyan M Wells
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, United States of America
PLoS Pathog 9:e1003120. 2013..In conclusion, this study identified the first components of novel siderophore export systems which are essential for virulence of Mtb... - MspA nanopores from subunit dimersMikhail Pavlenok
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, United States of America
PLoS ONE 7:e38726. 2012..This approach will be valuable both in understanding transport across the outer membrane in mycobacteria and in tailoring MspA for nanopore sequencing of DNA... - Hit-and-run stimulation: a novel concept to reactivate latent HIV-1 infection without cytokine gene inductionFrank Wolschendorf
Department of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J Virol 84:8712-20. 2010..In the absence of such a positive feedback mechanism, cellular gene expression was not sustained, suggesting that strategies modulating the NF-kappaB activity profile could be used to selectively trigger HIV-1 reactivation... - Taking phage integration to the next level as a genetic tool for mycobacteriaJason Huff
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
Gene 468:8-19. 2010..tuberculosis strains with multiple integrations of gfp increased concomitantly with the copy number demonstrating that these vectors can be used to generate stronger phenotypes and/or to analyze several genes simultaneously in vivo... - Multidrug resistance of a porin deletion mutant of Mycobacterium smegmatisJoachim Stephan
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th St. South, Birmingham, AL 35294, USA
Antimicrob Agents Chemother 48:4163-70. 2004..smegmatis to antibiotics. An understanding of the pathways across the outer membrane is essential to the successful design of chemotherapeutic agents with activities against mycobacteria... - Expression of the major porin gene mspA is regulated in Mycobacterium smegmatisDietmar Hillmann
Department of Microbiology, University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA
J Bacteriol 189:958-67. 2007..These results show for the first time that M. smegmatis regulates porin gene expression to optimize uptake of certain nutrients and to protect itself from toxic solutes... - Identification and semi-quantitative analysis of Mycobacterium tuberculosis H37Rv ftsZ gene-specific promoter activity-containing regionsSougata Roy
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India
Res Microbiol 155:817-26. 2004..tuberculosis quantitatively confirmed these promoter activities. Thus, at least three independent regions in the immediate upstream sequence of MtftsZ contain promoter activity, with the major contribution coming from ftsQ ORF... - A genomic view of sugar transport in Mycobacterium smegmatis and Mycobacterium tuberculosisFritz Titgemeyer
Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstrasse 5, D 91058 Erlangen, Germany
J Bacteriol 189:5903-15. 2007..smegmatis and M. tuberculosis in their natural habitats, the soil and the human body, respectively... - The core of the tetrameric mycobacterial porin MspA is an extremely stable beta-sheet domainChristian Heinz
, , Staudtstr. 5, Germany
J Biol Chem 278:8678-85. 2003..Thus, MspA may be of special interest for biotechnological applications... - The structure of a mycobacterial outer-membrane channelMichael Faller
Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstrasse 21, 79104 Freiburg im Breisgau, Germany
Science 303:1189-92. 2004..MspA contains two consecutive beta barrels with nonpolar outer surfaces that form a ribbon around the porin, which is too narrow to fit the thickness of the mycobacterial outer membrane in contemporary models... - The MspA porin promotes growth and increases antibiotic susceptibility of both Mycobacterium bovis BCG and Mycobacterium tuberculosisClaudia Mailaender
, , Staudtstr 5, D-91058 Erlangen, Germany
Microbiology 150:853-64. 2004..This study provides the first experimental evidence that porins are important for drug susceptibility of M. tuberculosis... - Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinaseJoachim Stephan
, , Staudtstr. 5, D-91058 Erlangen, Germany
Gene 343:181-90. 2004..These results show that the Flp/FRT system is a suitable genetic tool for constructing unmarked mutations and for the analysis of redundant genes by consecutive gene deletions in M. smegmatis... - Disclosure of the mycobacterial outer membrane: cryo-electron tomography and vitreous sections reveal the lipid bilayer structureChristian Hoffmann
Max Planck Institut fur Biochemie, Abteilung Molekulare Strukturbiologie, D 82152 Martinsried, Germany
Proc Natl Acad Sci U S A 105:3963-7. 2008..These results are crucial for the investigation and understanding of transport processes across the mycobacterial cell wall, and they are of particular medical relevance in the case of pathogenic mycobacteria... - Porins limit the intracellular persistence of Mycobacterium smegmatisSoroush Sharbati-Tehrani
Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany
Microbiology 151:2403-10. 2005..smegmatis depends upon the permeability of the outer membrane... - The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrientsJoachim Stephan
Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstrasse 5, D 91058 Erlangen, Germany
Mol Microbiol 58:714-30. 2005..smegmatis dropped drastically with its porin-mediated OM permeability in contrast to porin mutants of Escherichia coli. These results show that porin-mediated influx of nutrients is a major determinant of the growth rate of M. smegmatis... - Topology of the porin MspA in the outer membrane of Mycobacterium smegmatisMaysa Mahfoud
, , Staudtstrasse 5, D-91058 Erlangen, Germany
J Biol Chem 281:5908-15. 2006..4-nm long part of the hydrophilic rim domain are embedded into the OM of M. smegmatis. This is the first report suggesting that elements other than hydrophobic alpha-helices or beta-sheets are integrated into a lipid membrane... - Characterization of nanostructured surfaces generated by reconstitution of the porin MspA from Mycobacterium smegmatisMichael Wörner
Department of Chemical and Process Engineering, University of Karlsruhe, 76128 Karlsruhe, Germany
Small 3:1084-97. 2007.... - A tetrameric porin limits the cell wall permeability of Mycobacterium smegmatisHarald Engelhardt
, Abteilung Molekulare Strukturbiologie, Am Klopferspitz 18a, D-82152 Martinsried, Germany
J Biol Chem 277:37567-72. 2002..The length of MspA is sufficient to span the outer membrane and contributes in combination with the tapering end of the pore and the low number of pores to the low permeability of the cell wall of M. smegmatis for hydrophilic compounds...
Research Grants
- Copper transport in Mycobacterium tuberculosisMichael Niederweis; Fiscal Year: 2010..tuberculosis is required for efflux of copper. This protein is surface-accessible and represents the first outer membrane component of any efflux system in mycobacteria. ..
- Porins of Mycobacterium tuberculosisMichael Niederweis; Fiscal Year: 2009..These studies will not only be essential for the design of new TB drugs, but will also be of fundamental interest for our understanding of nutrient uptake by M. tuberculosis. ..