Wayne L Nicholson

Summary

Affiliation: University of Florida
Country: USA

Publications

  1. ncbi request reprint The solar UV environment and bacterial spore UV resistance: considerations for Earth-to-Mars transport by natural processes and human spaceflight
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Mail Code UF 1, Building M6 1025 SLSL, Kennedy Space Center, FL 32899, USA
    Mutat Res 571:249-64. 2005
  2. pmc Growth of Carnobacterium spp. from permafrost under low pressure, temperature, and anoxic atmosphere has implications for Earth microbes on Mars
    Wayne L Nicholson
    Department of Microbiology and Cell Science, Space Life Sciences Laboratory, University of Florida, Merritt Island, FL 32953, USA
    Proc Natl Acad Sci U S A 110:666-71. 2013
  3. doi request reprint Transcriptomic responses of germinating Bacillus subtilis spores exposed to 1.5 years of space and simulated martian conditions on the EXPOSE-E experiment PROTECT
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
    Astrobiology 12:469-86. 2012
  4. pmc Increased competitive fitness of Bacillus subtilis under nonsporulating conditions via inactivation of pleiotropic regulators AlsR, SigD, and SigW
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA
    Appl Environ Microbiol 78:3500-3. 2012
  5. doi request reprint Carbon-13 (13C) labeling of Bacillus subtilis vegetative cells and spores: suitability for DNA stable isotope probing (DNA-SIP) of spores in soils
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
    Curr Microbiol 59:9-14. 2009
  6. ncbi request reprint Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermia
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
    Astrobiology 5:536-44. 2005
  7. pmc The Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2,3-butanediol dehydrogenase
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
    Appl Environ Microbiol 74:6832-8. 2008
  8. doi request reprint Ancient micronauts: interplanetary transport of microbes by cosmic impacts
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Building M6 1025, Room 201 B, Kennedy Space Center, FL 32899, USA
    Trends Microbiol 17:243-50. 2009
  9. doi request reprint Migrating microbes and planetary protection
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, FL 32899, USA
    Trends Microbiol 17:389-92. 2009
  10. doi request reprint The O/OREOS mission: first science data from the Space Environment Survivability of Living Organisms (SESLO) payload
    Wayne L Nicholson
    University of Florida, Kennedy Space Center, Florida, USA
    Astrobiology 11:951-8. 2011

Collaborators

  • Peter Setlow
  • Andrew C Schuerger
  • Ralf Moeller
  • David Gilichinsky
  • Pascale Ehrenfreund
  • Thomas Berger
  • Petra Rettberg
  • Heather Maughan
  • E Stackebrandt
  • K Venkateswaran
  • Thierry Douki
  • Joanna Masel
  • Patricia Fajardo-Cavazos
  • Amy E Perkins
  • Christopher T Brown
  • Paul E Hintze
  • H Jay Melosh
  • Jason Gioia
  • Courtney Tauscher
  • James N Benardini
  • Marilyn M Marshall
  • Belinda Galeano
  • Samantha M Waters
  • James J Marois
  • Sheeja George
  • Binna M Chokshi
  • Tracy N Wright
  • William J Zaragoza
  • Alison T Rioux
  • Eric W Triplett
  • Marissa A Cuff
  • Enrique Rodriguez
  • James S Woollcombe-Clarke
  • Travis Oglesby
  • Jennifer C Drew
  • Laura K Fishwick
  • Austin H Trupp
  • Gregory S Stupp
  • Jay M Jackson
  • Falko Langenhorst
  • Wen Liu
  • Lina Bokhetache
  • George E Fox
  • Peter Blyth
  • Lisa Golebiewski
  • Yamei Liu
  • George M Weinstock
  • Madhan R Tirumalai
  • Huaiyang Jiang
  • Avani Verma
  • David Newcombe
  • Hiba Zwiya
  • Prince Buzumbo
  • Lesette Perez
  • Michael Holder
  • Donna Muzny
  • Lynne Nazareth
  • Kenneth D Clinkenbeard
  • Indrani Dasgupta
  • Joseph F Petrosino
  • Prahathees Eswara Moorthy
  • Adeloa Olowu
  • Sarah K Highlander
  • Sandra Lee
  • Christian Buhay
  • Fathi Karouia
  • Alicia Hawes
  • Xiang Qin
  • Brian D McWilliams
  • Shannon Dugan-Rocha
  • Akif Uzman
  • Shailaja Yerrapragada
  • Huyen Dinh
  • Yan Ding
  • Masaya Fujita
  • Okezie C Igboeli
  • Christie Kovar
  • Johnathan Siefert
  • Roberto Rebeil
  • Lindsey Link
  • Emily Korff
  • Charles R Sterling
  • Jackie Moffett
  • Samuel Hayes
  • John Sawyer

Detail Information

Publications41

  1. ncbi request reprint The solar UV environment and bacterial spore UV resistance: considerations for Earth-to-Mars transport by natural processes and human spaceflight
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Mail Code UF 1, Building M6 1025 SLSL, Kennedy Space Center, FL 32899, USA
    Mutat Res 571:249-64. 2005
    ....
  2. pmc Growth of Carnobacterium spp. from permafrost under low pressure, temperature, and anoxic atmosphere has implications for Earth microbes on Mars
    Wayne L Nicholson
    Department of Microbiology and Cell Science, Space Life Sciences Laboratory, University of Florida, Merritt Island, FL 32953, USA
    Proc Natl Acad Sci U S A 110:666-71. 2013
    ..gallinarum, C. inhibens, C. maltaromaticum, C. mobile, C. pleistocenium, and C. viridans) were all capable of growth under cold, low-pressure, anoxic conditions, thus extending the low-pressure extreme at which life can function...
  3. doi request reprint Transcriptomic responses of germinating Bacillus subtilis spores exposed to 1.5 years of space and simulated martian conditions on the EXPOSE-E experiment PROTECT
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
    Astrobiology 12:469-86. 2012
    ..The results are discussed in the context of planetary protection for a hypothetical journey of potential forward contaminant spores from Earth to Mars and their subsequent residence on Mars...
  4. pmc Increased competitive fitness of Bacillus subtilis under nonsporulating conditions via inactivation of pleiotropic regulators AlsR, SigD, and SigW
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA
    Appl Environ Microbiol 78:3500-3. 2012
    ..The fitness of strains carrying knockout mutations alsR::spc, sigD::kan, and/or sigW::erm was measured and compared to that of the congenic ancestral strain by competition experiments...
  5. doi request reprint Carbon-13 (13C) labeling of Bacillus subtilis vegetative cells and spores: suitability for DNA stable isotope probing (DNA-SIP) of spores in soils
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
    Curr Microbiol 59:9-14. 2009
    ..336 at.% (13)C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing (12)C versus (13)C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients...
  6. ncbi request reprint Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermia
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
    Astrobiology 5:536-44. 2005
    ..However, germination-induced bioluminescence was lower in spores exposed to simulated martian atmosphere, which suggests sublethal impairment of some endogenous spore germination processes...
  7. pmc The Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2,3-butanediol dehydrogenase
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
    Appl Environ Microbiol 74:6832-8. 2008
    ..From the data, it is proposed that the major AR/BDH-encoding gene ydjL be renamed bdhA...
  8. doi request reprint Ancient micronauts: interplanetary transport of microbes by cosmic impacts
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Building M6 1025, Room 201 B, Kennedy Space Center, FL 32899, USA
    Trends Microbiol 17:243-50. 2009
    ..Here, I summarize our current understanding of the physics of impacts, space transport of meteorites, and the potentiality of microorganisms to undergo and survive interplanetary transfer...
  9. doi request reprint Migrating microbes and planetary protection
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, FL 32899, USA
    Trends Microbiol 17:389-92. 2009
    ..As plans proceed for future life detection and human exploration missions, planetary protection considerations are again uppermost on the agendas of mission planners, and microbiologists have an important role to play...
  10. doi request reprint The O/OREOS mission: first science data from the Space Environment Survivability of Living Organisms (SESLO) payload
    Wayne L Nicholson
    University of Florida, Kennedy Space Center, Florida, USA
    Astrobiology 11:951-8. 2011
    ..We report here on the evaluation and interpretation of these spaceflight data in comparison to delayed-synchronous laboratory ground control experiments...
  11. doi request reprint Exposure of DNA and Bacillus subtilis spores to simulated martian environments: use of quantitative PCR (qPCR) to measure inactivation rates of DNA to function as a template molecule
    Patricia Fajardo-Cavazos
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
    Astrobiology 10:403-11. 2010
    ..The results indicate that forward-contaminant DNA could persist for considerable periods of time at the martian surface...
  12. pmc Exploring the low-pressure growth limit: evolution of Bacillus subtilis in the laboratory to enhanced growth at 5 kilopascals
    Wayne L Nicholson
    Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
    Appl Environ Microbiol 76:7559-65. 2010
    ..3 kPa. The results from this preliminary study have implications for understanding the ability of terrestrial microbes to grow in low-pressure environments such as Mars...
  13. pmc Persistence of biomarker ATP and ATP-generating capability in bacterial cells and spores contaminating spacecraft materials under earth conditions and in a simulated martian environment
    Patricia Fajardo-Cavazos
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, FL 32899, USA
    Appl Environ Microbiol 74:5159-67. 2008
    ....
  14. doi request reprint Bacterial spores in granite survive hypervelocity launch by spallation: implications for lithopanspermia
    Patricia Fajardo-Cavazos
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, Florida 32899, USA
    Astrobiology 9:647-57. 2009
    ..These results demonstrate that endolithic spores can survive launch by spallation from a hypervelocity impact, which lends further evidence in favor of lithopanspermia theory...
  15. ncbi request reprint Survival and germinability of Bacillus subtilis spores exposed to simulated Mars solar radiation: implications for life detection and planetary protection
    Courtney Tauscher
    Department of Microbiology, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, Florida 32899, USA
    Astrobiology 6:592-605. 2006
    ..The data indicate that spores can retain the potential to initiate germination-associated metabolic processes and produce biological signature molecules after being rendered nonviable by exposure to Mars solar radiation...
  16. doi request reprint Isolation of rpoB mutations causing rifampicin resistance in Bacillus subtilis spores exposed to simulated Martian surface conditions
    Amy E Perkins
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
    Astrobiology 8:1159-67. 2008
    ..the MSC. However, both spectra are distinctly different from Rif(R) mutations previously reported arising from B. subtilis spores exposed to simulated space vacuum...
  17. doi request reprint Evolution of Bacillus subtilis to enhanced growth at low pressure: up-regulated transcription of des-desKR, encoding the fatty acid desaturase system
    Patricia Fajardo-Cavazos
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, 32899, USA
    Astrobiology 12:258-70. 2012
    ..The present study represents a first step toward identification of molecular mechanisms by which B. subtilis could sense and respond to the novel environmental stress of low pressure...
  18. pmc Growth of Serratia liquefaciens under 7 mbar, 0°C, and CO2-enriched anoxic atmospheres
    Andrew C Schuerger
    Department of Plant Pathology, University of Florida, Space Life Sciences Lab, Kennedy Space Center, Florida 32899, USA
    Astrobiology 13:115-31. 2013
    ..1% ppO2; both species) conditions...
  19. ncbi request reprint Bacillus subtilis spores on artificial meteorites survive hypervelocity atmospheric entry: implications for Lithopanspermia
    Patricia Fajardo-Cavazos
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
    Astrobiology 5:726-36. 2005
    ....
  20. pmc Role of DNA repair by nonhomologous-end joining in Bacillus subtilis spore resistance to extreme dryness, mono- and polychromatic UV, and ionizing radiation
    Ralf Moeller
    Space Life Sciences Laboratory, Building M6 1025 SLSL, Kennedy Space Center, FL 32953, USA
    J Bacteriol 189:3306-11. 2007
    ....
  21. pmc Increased fitness and alteration of metabolic pathways during Bacillus subtilis evolution in the laboratory
    Heather Maughan
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    Appl Environ Microbiol 77:4105-18. 2011
    ..The results suggested that the derived colony morphology of strain WN716 was associated with increased fitness, the alteration of several metabolic pathways, and the loss of a typical postexponential-phase response...
  22. doi request reprint Single-spore elemental analyses indicate that dipicolinic acid-deficient Bacillus subtilis spores fail to accumulate calcium
    Paul E Hintze
    NASA Corrosion Technology Laboratory, Kennedy Space Center, Gainesville, FL 32899, USA
    Arch Microbiol 192:493-7. 2010
    ....
  23. pmc Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation
    Christopher T Brown
    Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA
    Appl Environ Microbiol 77:6867-77. 2011
    ..The results suggest that propagation of B. subtilis for less than 2,000 generations in a nutrient-rich environment where sporulation is suppressed led to rapid initiation of genomic erosion...
  24. pmc Uncovering new metabolic capabilities of Bacillus subtilis using phenotype profiling of rifampin-resistant rpoB mutants
    Amy E Perkins
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
    J Bacteriol 190:807-14. 2008
    ..subtilis...
  25. ncbi request reprint Essential cysteine residues in Bacillus subtilis spore photoproduct lyase identified by alanine scanning mutagenesis
    Patricia Fajardo-Cavazos
    Department of Microbiology and Cell Science, University of Florida, Room 201 B Space Life Sciences Laboratory, Building M6 1025 SLSL, Kennedy Space Center, FL 32899, USA
    Curr Microbiol 51:331-5. 2005
    ....
  26. ncbi request reprint Bacterial endospores and their significance in stress resistance
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
    Antonie Van Leeuwenhoek 81:27-32. 2002
    ..The current data are reviewed with the ultimate goal of obtaining a complete model describing spore persistence and longevity in the terrestrial solar UV radiation environment...
  27. pmc Comparison of UV inactivation of spores of three encephalitozoon species with that of spores of two DNA repair-deficient Bacillus subtilis biodosimetry strains
    Marilyn M Marshall
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Appl Environ Microbiol 69:683-5. 2003
    ..The results indicate that spores of Encephalitozoon spp. are readily inactivated at low UV fluences and that spores of UV-sensitive B. subtilis strains can be useful surrogates in evaluating UV reactor performance...
  28. pmc UV resistance of Bacillus anthracis spores revisited: validation of Bacillus subtilis spores as UV surrogates for spores of B. anthracis Sterne
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Appl Environ Microbiol 69:1327-30. 2003
    ..subtilis spores are likely also sufficient to inactivate B. anthracis spores and that the spores of standard B. subtilis strains could reliably be used as a biodosimetry model for the UV inactivation of B. anthracis spores...
  29. pmc Inactivation of vegetative cells, but not spores, of Bacillus anthracis, B. cereus, and B. subtilis on stainless steel surfaces coated with an antimicrobial silver- and zinc-containing zeolite formulation
    Belinda Galeano
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
    Appl Environ Microbiol 69:4329-31. 2003
    ....
  30. ncbi request reprint Spore UV and acceleration resistance of endolithic Bacillus pumilus and Bacillus subtilis isolates obtained from Sonoran desert basalt: implications for lithopanspermia
    James N Benardini
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona, USA
    Astrobiology 3:709-17. 2003
    ..subtilis, the benchmark organism, suggesting that spores of environmental B. pumilus isolates may be more likely to survive the rigors of interplanetary transfer...
  31. ncbi request reprint UV-radiation-induced formation of DNA bipyrimidine photoproducts in Bacillus subtilis endospores and their repair during germination
    Ralf Moeller
    German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany
    Int Microbiol 10:39-46. 2007
    ..SP-lyase-deficient spores repaired 20% of the SP produced. Thus, SP lyase, with respect to nucleotide excision repair, has a remarkable impact on the removal of SP upon spore germination...
  32. pmc Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032
    Jason Gioia
    Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, USA
    PLoS ONE 2:e928. 2007
    ....
  33. ncbi request reprint UV photochemistry of DNA in vitro and in Bacillus subtilis spores at earth-ambient and low atmospheric pressure: implications for spore survival on other planets or moons in the solar system
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Astrobiology 2:417-25. 2002
    ....
  34. pmc The spectrum of spontaneous rifampin resistance mutations in the rpoB gene of Bacillus subtilis 168 spores differs from that of vegetative cells and resembles that of Mycobacterium tuberculosis
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
    J Bacteriol 184:4936-40. 2002
    ..The observations are discussed in terms of the underlying differences of the DNA environment within dormant cells and vegetatively growing cells...
  35. ncbi request reprint The population genetics of phenotypic deterioration in experimental populations of Bacillus subtilis
    Heather Maughan
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
    Evolution 60:686-95. 2006
    ..e., prototrophy) was lost in all populations regardless of the experimental environment and that the pattern of trait loss in one environment was due to selection, whereas in the other environment the cause remains inconclusive...
  36. pmc Roles of the major, small, acid-soluble spore proteins and spore-specific and universal DNA repair mechanisms in resistance of Bacillus subtilis spores to ionizing radiation from X rays and high-energy charged-particle bombardment
    Ralf Moeller
    German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Department of Photo and Exobiology, Cologne, Germany
    J Bacteriol 190:1134-40. 2008
    ....
  37. pmc The roles of mutation accumulation and selection in loss of sporulation in experimental populations of Bacillus subtilis
    Heather Maughan
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
    Genetics 177:937-48. 2007
    ..These results are discussed in the context of the evolution of sporulation in particular and phenotypic loss in general...
  38. pmc Novel rpoB mutations conferring rifampin resistance on Bacillus subtilis: global effects on growth, competence, sporulation, and germination
    Heather Maughan
    Departments of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
    J Bacteriol 186:2481-6. 2004
    ..The results thus establish the important role played by the RNA polymerase beta subunit, not only in the catalytic aspect of transcription, but also in the regulation of major developmental events in B. subtilis...
  39. pmc Stochastic processes influence stationary-phase decisions in Bacillus subtilis
    Heather Maughan
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
    J Bacteriol 186:2212-4. 2004
    ..In support of the noise hypothesis, we report that none of the populations responded to selection by improving their efficiency of sporulation, indicating that intracellular noise is independent of heritable genotype...
  40. ncbi request reprint Using thermal inactivation kinetics to calculate the probability of extreme spore longevity: implications for paleomicrobiology and lithopanspermia
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ, USA
    Orig Life Evol Biosph 33:621-31. 2003
    ..The significance of the results are discussed in terms of the survival probabilities of (i) terrestrial spores in ancient geologic samples and (ii) spores transported between planets within impact ejecta...
  41. ncbi request reprint The paradox of the "ancient" bacterium which contains "modern" protein-coding genes
    Heather Maughan
    Mol Biol Evol 19:1637-9. 2002