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Genomes and Genes | Wayne L NicholsonSummaryAffiliation: University of Florida Country: USA Publications
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The solar UV environment and bacterial spore UV resistance: considerations for Earth-to-Mars transport by natural processes and human spaceflightWayne L Nicholson
Department of Microbiology and Cell Science, University of Florida, Mail Code UF 1, Building M6 1025 SLSL, Kennedy Space Center, FL 32899, USA
Mutat Res 571:249-64. 2005....
Growth of Carnobacterium spp. from permafrost under low pressure, temperature, and anoxic atmosphere has implications for Earth microbes on MarsWayne L Nicholson
Department of Microbiology and Cell Science, Space Life Sciences Laboratory, University of Florida, Merritt Island, FL 32953, USA
Proc Natl Acad Sci U S A 110:666-71. 2013..gallinarum, C. inhibens, C. maltaromaticum, C. mobile, C. pleistocenium, and C. viridans) were all capable of growth under cold, low-pressure, anoxic conditions, thus extending the low-pressure extreme at which life can function...
Transcriptomic responses of germinating Bacillus subtilis spores exposed to 1.5 years of space and simulated martian conditions on the EXPOSE-E experiment PROTECTWayne L Nicholson
Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
Astrobiology 12:469-86. 2012..The results are discussed in the context of planetary protection for a hypothetical journey of potential forward contaminant spores from Earth to Mars and their subsequent residence on Mars...- Increased competitive fitness of Bacillus subtilis under nonsporulating conditions via inactivation of pleiotropic regulators AlsR, SigD, and SigWWayne L Nicholson
Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA
Appl Environ Microbiol 78:3500-3. 2012..The fitness of strains carrying knockout mutations alsR::spc, sigD::kan, and/or sigW::erm was measured and compared to that of the congenic ancestral strain by competition experiments... - The O/OREOS mission: first science data from the Space Environment Survivability of Living Organisms (SESLO) payloadWayne L Nicholson
University of Florida, Kennedy Space Center, Florida, USA
Astrobiology 11:951-8. 2011..We report here on the evaluation and interpretation of these spaceflight data in comparison to delayed-synchronous laboratory ground control experiments... - The Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2,3-butanediol dehydrogenaseWayne L Nicholson
Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
Appl Environ Microbiol 74:6832-8. 2008..From the data, it is proposed that the major AR/BDH-encoding gene ydjL be renamed bdhA... - Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermiaWayne L Nicholson
Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
Astrobiology 5:536-44. 2005..However, germination-induced bioluminescence was lower in spores exposed to simulated martian atmosphere, which suggests sublethal impairment of some endogenous spore germination processes... - Exploring the low-pressure growth limit: evolution of Bacillus subtilis in the laboratory to enhanced growth at 5 kilopascalsWayne L Nicholson
Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
Appl Environ Microbiol 76:7559-65. 2010..3 kPa. The results from this preliminary study have implications for understanding the ability of terrestrial microbes to grow in low-pressure environments such as Mars... - Carbon-13 (13C) labeling of Bacillus subtilis vegetative cells and spores: suitability for DNA stable isotope probing (DNA-SIP) of spores in soilsWayne L Nicholson
Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
Curr Microbiol 59:9-14. 2009..336 at.% (13)C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing (12)C versus (13)C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients... - Exposure of DNA and Bacillus subtilis spores to simulated martian environments: use of quantitative PCR (qPCR) to measure inactivation rates of DNA to function as a template moleculePatricia Fajardo-Cavazos
Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
Astrobiology 10:403-11. 2010..The results indicate that forward-contaminant DNA could persist for considerable periods of time at the martian surface... - Ancient micronauts: interplanetary transport of microbes by cosmic impactsWayne L Nicholson
Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Building M6 1025, Room 201 B, Kennedy Space Center, FL 32899, USA
Trends Microbiol 17:243-50. 2009..Here, I summarize our current understanding of the physics of impacts, space transport of meteorites, and the potentiality of microorganisms to undergo and survive interplanetary transfer... - Migrating microbes and planetary protectionWayne L Nicholson
Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, FL 32899, USA
Trends Microbiol 17:389-92. 2009..As plans proceed for future life detection and human exploration missions, planetary protection considerations are again uppermost on the agendas of mission planners, and microbiologists have an important role to play... - Persistence of biomarker ATP and ATP-generating capability in bacterial cells and spores contaminating spacecraft materials under earth conditions and in a simulated martian environmentPatricia Fajardo-Cavazos
Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, FL 32899, USA
Appl Environ Microbiol 74:5159-67. 2008.... - Bacterial spores in granite survive hypervelocity launch by spallation: implications for lithopanspermiaPatricia Fajardo-Cavazos
Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, Florida 32899, USA
Astrobiology 9:647-57. 2009..These results demonstrate that endolithic spores can survive launch by spallation from a hypervelocity impact, which lends further evidence in favor of lithopanspermia theory... - Isolation of rpoB mutations causing rifampicin resistance in Bacillus subtilis spores exposed to simulated Martian surface conditionsAmy E Perkins
Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
Astrobiology 8:1159-67. 2008..the MSC. However, both spectra are distinctly different from Rif(R) mutations previously reported arising from B. subtilis spores exposed to simulated space vacuum... - Survival and germinability of Bacillus subtilis spores exposed to simulated Mars solar radiation: implications for life detection and planetary protectionCourtney Tauscher
Department of Microbiology, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, Florida 32899, USA
Astrobiology 6:592-605. 2006..The data indicate that spores can retain the potential to initiate germination-associated metabolic processes and produce biological signature molecules after being rendered nonviable by exposure to Mars solar radiation... - Evolution of Bacillus subtilis to enhanced growth at low pressure: up-regulated transcription of des-desKR, encoding the fatty acid desaturase systemPatricia Fajardo-Cavazos
Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, 32899, USA
Astrobiology 12:258-70. 2012..The present study represents a first step toward identification of molecular mechanisms by which B. subtilis could sense and respond to the novel environmental stress of low pressure... - Bacillus subtilis spores on artificial meteorites survive hypervelocity atmospheric entry: implications for LithopanspermiaPatricia Fajardo-Cavazos
Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
Astrobiology 5:726-36. 2005.... - Role of DNA repair by nonhomologous-end joining in Bacillus subtilis spore resistance to extreme dryness, mono- and polychromatic UV, and ionizing radiationRalf Moeller
Space Life Sciences Laboratory, Building M6 1025 SLSL, Kennedy Space Center, FL 32953, USA
J Bacteriol 189:3306-11. 2007.... - Increased fitness and alteration of metabolic pathways during Bacillus subtilis evolution in the laboratoryHeather Maughan
Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
Appl Environ Microbiol 77:4105-18. 2011..The results suggested that the derived colony morphology of strain WN716 was associated with increased fitness, the alteration of several metabolic pathways, and the loss of a typical postexponential-phase response... - Single-spore elemental analyses indicate that dipicolinic acid-deficient Bacillus subtilis spores fail to accumulate calciumPaul E Hintze
NASA Corrosion Technology Laboratory, Kennedy Space Center, Gainesville, FL 32899, USA
Arch Microbiol 192:493-7. 2010.... - Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulationChristopher T Brown
Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA
Appl Environ Microbiol 77:6867-77. 2011..The results suggest that propagation of B. subtilis for less than 2,000 generations in a nutrient-rich environment where sporulation is suppressed led to rapid initiation of genomic erosion... - Growth of Serratia liquefaciens under 7 mbar, 0°C, and CO2-enriched anoxic atmospheresAndrew C Schuerger
Department of Plant Pathology, University of Florida, Space Life Sciences Lab, Kennedy Space Center, Florida 32899, USA
Astrobiology 13:115-31. 2013..1% ppO2; both species) conditions... - Uncovering new metabolic capabilities of Bacillus subtilis using phenotype profiling of rifampin-resistant rpoB mutantsAmy E Perkins
Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
J Bacteriol 190:807-14. 2008..subtilis... - Essential cysteine residues in Bacillus subtilis spore photoproduct lyase identified by alanine scanning mutagenesisPatricia Fajardo-Cavazos
Department of Microbiology and Cell Science, University of Florida, Room 201-B Space Life Sciences Laboratory, Building M6-1025/SLSL, Kennedy Space Center, FL 32899, USA
Curr Microbiol 51:331-5. 2005.... - Bacterial endospores and their significance in stress resistanceWayne L Nicholson
Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
Antonie Van Leeuwenhoek 81:27-32. 2002..The current data are reviewed with the ultimate goal of obtaining a complete model describing spore persistence and longevity in the terrestrial solar UV radiation environment... - Comparison of UV inactivation of spores of three encephalitozoon species with that of spores of two DNA repair-deficient Bacillus subtilis biodosimetry strainsMarilyn M Marshall
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
Appl Environ Microbiol 69:683-5. 2003..The results indicate that spores of Encephalitozoon spp. are readily inactivated at low UV fluences and that spores of UV-sensitive B. subtilis strains can be useful surrogates in evaluating UV reactor performance... - UV resistance of Bacillus anthracis spores revisited: validation of Bacillus subtilis spores as UV surrogates for spores of B. anthracis SterneWayne L Nicholson
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
Appl Environ Microbiol 69:1327-30. 2003..subtilis spores are likely also sufficient to inactivate B. anthracis spores and that the spores of standard B. subtilis strains could reliably be used as a biodosimetry model for the UV inactivation of B. anthracis spores... - Inactivation of vegetative cells, but not spores, of Bacillus anthracis, B. cereus, and B. subtilis on stainless steel surfaces coated with an antimicrobial silver- and zinc-containing zeolite formulationBelinda Galeano
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
Appl Environ Microbiol 69:4329-31. 2003.... - Spore UV and acceleration resistance of endolithic Bacillus pumilus and Bacillus subtilis isolates obtained from Sonoran desert basalt: implications for lithopanspermiaJames N Benardini
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona, USA
Astrobiology 3:709-17. 2003..subtilis, the benchmark organism, suggesting that spores of environmental B. pumilus isolates may be more likely to survive the rigors of interplanetary transfer... - Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032Jason Gioia
Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, USA
PLoS ONE 2:e928. 2007.... - UV-radiation-induced formation of DNA bipyrimidine photoproducts in Bacillus subtilis endospores and their repair during germinationRalf Moeller
German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany
Int Microbiol 10:39-46. 2007..SP-lyase-deficient spores repaired 20% of the SP produced. Thus, SP lyase, with respect to nucleotide excision repair, has a remarkable impact on the removal of SP upon spore germination... - UV photochemistry of DNA in vitro and in Bacillus subtilis spores at earth-ambient and low atmospheric pressure: implications for spore survival on other planets or moons in the solar systemWayne L Nicholson
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
Astrobiology 2:417-25. 2002.... - The spectrum of spontaneous rifampin resistance mutations in the rpoB gene of Bacillus subtilis 168 spores differs from that of vegetative cells and resembles that of Mycobacterium tuberculosisWayne L Nicholson
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
J Bacteriol 184:4936-40. 2002..The observations are discussed in terms of the underlying differences of the DNA environment within dormant cells and vegetatively growing cells... - The population genetics of phenotypic deterioration in experimental populations of Bacillus subtilisHeather Maughan
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
Evolution 60:686-95. 2006..e., prototrophy) was lost in all populations regardless of the experimental environment and that the pattern of trait loss in one environment was due to selection, whereas in the other environment the cause remains inconclusive... - Roles of the major, small, acid-soluble spore proteins and spore-specific and universal DNA repair mechanisms in resistance of Bacillus subtilis spores to ionizing radiation from X rays and high-energy charged-particle bombardmentRalf Moeller
German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Department of Photo and Exobiology, Cologne, Germany
J Bacteriol 190:1134-40. 2008.... - The roles of mutation accumulation and selection in loss of sporulation in experimental populations of Bacillus subtilisHeather Maughan
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
Genetics 177:937-48. 2007..These results are discussed in the context of the evolution of sporulation in particular and phenotypic loss in general... - Novel rpoB mutations conferring rifampin resistance on Bacillus subtilis: global effects on growth, competence, sporulation, and germinationHeather Maughan
Departments of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
J Bacteriol 186:2481-6. 2004..The results thus establish the important role played by the RNA polymerase beta subunit, not only in the catalytic aspect of transcription, but also in the regulation of major developmental events in B. subtilis... - Stochastic processes influence stationary-phase decisions in Bacillus subtilisHeather Maughan
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
J Bacteriol 186:2212-4. 2004..In support of the noise hypothesis, we report that none of the populations responded to selection by improving their efficiency of sporulation, indicating that intracellular noise is independent of heritable genotype... - Using thermal inactivation kinetics to calculate the probability of extreme spore longevity: implications for paleomicrobiology and lithopanspermiaWayne L Nicholson
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ, USA
Orig Life Evol Biosph 33:621-31. 2003..The significance of the results are discussed in terms of the survival probabilities of (i) terrestrial spores in ancient geologic samples and (ii) spores transported between planets within impact ejecta... - The paradox of the "ancient" bacterium which contains "modern" protein-coding genesHeather Maughan
Mol Biol Evol 19:1637-9. 2002