W L Nicholson

Summary

Affiliation: University of Arizona
Country: USA

Publications

  1. ncbi request reprint Analysis of spore photoproduct lyase operon (splAB) function using targeted deletion-insertion mutations spanning the Bacillus subtilis operons ptsHI and splAB
    W L Nicholson
    Dept of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
    Mol Gen Genet 255:587-94. 1997
  2. ncbi request reprint Using thermal inactivation kinetics to calculate the probability of extreme spore longevity: implications for paleomicrobiology and lithopanspermia
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ, USA
    Orig Life Evol Biosph 33:621-31. 2003
  3. ncbi request reprint UV photochemistry of DNA in vitro and in Bacillus subtilis spores at earth-ambient and low atmospheric pressure: implications for spore survival on other planets or moons in the solar system
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Astrobiology 2:417-25. 2002
  4. pmc UV resistance of Bacillus anthracis spores revisited: validation of Bacillus subtilis spores as UV surrogates for spores of B. anthracis Sterne
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Appl Environ Microbiol 69:1327-30. 2003
  5. ncbi request reprint Bacterial endospores and their significance in stress resistance
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
    Antonie Van Leeuwenhoek 81:27-32. 2002
  6. pmc The spectrum of spontaneous rifampin resistance mutations in the rpoB gene of Bacillus subtilis 168 spores differs from that of vegetative cells and resembles that of Mycobacterium tuberculosis
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
    J Bacteriol 184:4936-40. 2002
  7. ncbi request reprint Roles of Bacillus endospores in the environment
    W L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
    Cell Mol Life Sci 59:410-6. 2002
  8. ncbi request reprint Method for purification of bacterial endospores from soils: UV resistance of natural Sonoran desert soil populations of Bacillus spp. with reference to B. subtilis strain 168
    W L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
    J Microbiol Methods 35:13-21. 1999
  9. pmc Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments
    W L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Microbiol Mol Biol Rev 64:548-72. 2000
  10. pmc Role of dipicolinic acid in survival of Bacillus subtilis spores exposed to artificial and solar UV radiation
    T A Slieman
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Appl Environ Microbiol 67:1274-9. 2001

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Analysis of spore photoproduct lyase operon (splAB) function using targeted deletion-insertion mutations spanning the Bacillus subtilis operons ptsHI and splAB
    W L Nicholson
    Dept of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
    Mol Gen Genet 255:587-94. 1997
    ..The complementation system was used to explore the role of SP lyase in determining spore UV resistance...
  2. ncbi request reprint Using thermal inactivation kinetics to calculate the probability of extreme spore longevity: implications for paleomicrobiology and lithopanspermia
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ, USA
    Orig Life Evol Biosph 33:621-31. 2003
    ..The significance of the results are discussed in terms of the survival probabilities of (i) terrestrial spores in ancient geologic samples and (ii) spores transported between planets within impact ejecta...
  3. ncbi request reprint UV photochemistry of DNA in vitro and in Bacillus subtilis spores at earth-ambient and low atmospheric pressure: implications for spore survival on other planets or moons in the solar system
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Astrobiology 2:417-25. 2002
    ....
  4. pmc UV resistance of Bacillus anthracis spores revisited: validation of Bacillus subtilis spores as UV surrogates for spores of B. anthracis Sterne
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Appl Environ Microbiol 69:1327-30. 2003
    ..subtilis spores are likely also sufficient to inactivate B. anthracis spores and that the spores of standard B. subtilis strains could reliably be used as a biodosimetry model for the UV inactivation of B. anthracis spores...
  5. ncbi request reprint Bacterial endospores and their significance in stress resistance
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
    Antonie Van Leeuwenhoek 81:27-32. 2002
    ..The current data are reviewed with the ultimate goal of obtaining a complete model describing spore persistence and longevity in the terrestrial solar UV radiation environment...
  6. pmc The spectrum of spontaneous rifampin resistance mutations in the rpoB gene of Bacillus subtilis 168 spores differs from that of vegetative cells and resembles that of Mycobacterium tuberculosis
    Wayne L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
    J Bacteriol 184:4936-40. 2002
    ..The observations are discussed in terms of the underlying differences of the DNA environment within dormant cells and vegetatively growing cells...
  7. ncbi request reprint Roles of Bacillus endospores in the environment
    W L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
    Cell Mol Life Sci 59:410-6. 2002
    ..and their endospores in the environment is reviewed, with particular emphasis on soil ecology, host-symbiont and host-parasite interactions, and human exploitation of spores as biological control agents and probiotics...
  8. ncbi request reprint Method for purification of bacterial endospores from soils: UV resistance of natural Sonoran desert soil populations of Bacillus spp. with reference to B. subtilis strain 168
    W L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA
    J Microbiol Methods 35:13-21. 1999
    ....
  9. pmc Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments
    W L Nicholson
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Microbiol Mol Biol Rev 64:548-72. 2000
    ....
  10. pmc Role of dipicolinic acid in survival of Bacillus subtilis spores exposed to artificial and solar UV radiation
    T A Slieman
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Appl Environ Microbiol 67:1274-9. 2001
    ....
  11. pmc The subunit structure and catalytic mechanism of the Bacillus subtilis DNA repair enzyme spore photoproduct lyase
    R Rebeil
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
    Proc Natl Acad Sci U S A 98:9038-43. 2001
    ..SP lyase is unique in being the first and only DNA repair enzyme known to function via this novel enzymatic mechanism...
  12. pmc Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032
    Jason Gioia
    Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, USA
    PLoS ONE 2:e928. 2007
    ....
  13. ncbi request reprint The population genetics of phenotypic deterioration in experimental populations of Bacillus subtilis
    Heather Maughan
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
    Evolution 60:686-95. 2006
    ..e., prototrophy) was lost in all populations regardless of the experimental environment and that the pattern of trait loss in one environment was due to selection, whereas in the other environment the cause remains inconclusive...
  14. ncbi request reprint Survival and germinability of Bacillus subtilis spores exposed to simulated Mars solar radiation: implications for life detection and planetary protection
    Courtney Tauscher
    Department of Microbiology, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, Florida 32899, USA
    Astrobiology 6:592-605. 2006
    ..The data indicate that spores can retain the potential to initiate germination-associated metabolic processes and produce biological signature molecules after being rendered nonviable by exposure to Mars solar radiation...
  15. pmc Role of DNA repair by nonhomologous-end joining in Bacillus subtilis spore resistance to extreme dryness, mono- and polychromatic UV, and ionizing radiation
    Ralf Moeller
    Space Life Sciences Laboratory, Building M6 1025 SLSL, Kennedy Space Center, FL 32953, USA
    J Bacteriol 189:3306-11. 2007
    ....
  16. ncbi request reprint UV-radiation-induced formation of DNA bipyrimidine photoproducts in Bacillus subtilis endospores and their repair during germination
    Ralf Moeller
    German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany
    Int Microbiol 10:39-46. 2007
    ..SP-lyase-deficient spores repaired 20% of the SP produced. Thus, SP lyase, with respect to nucleotide excision repair, has a remarkable impact on the removal of SP upon spore germination...
  17. pmc Uncovering new metabolic capabilities of Bacillus subtilis using phenotype profiling of rifampin-resistant rpoB mutants
    Amy E Perkins
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
    J Bacteriol 190:807-14. 2008
    ..subtilis...
  18. pmc Roles of the major, small, acid-soluble spore proteins and spore-specific and universal DNA repair mechanisms in resistance of Bacillus subtilis spores to ionizing radiation from X rays and high-energy charged-particle bombardment
    Ralf Moeller
    German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Department of Photo and Exobiology, Cologne, Germany
    J Bacteriol 190:1134-40. 2008
    ....
  19. pmc The roles of mutation accumulation and selection in loss of sporulation in experimental populations of Bacillus subtilis
    Heather Maughan
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
    Genetics 177:937-48. 2007
    ..These results are discussed in the context of the evolution of sporulation in particular and phenotypic loss in general...
  20. ncbi request reprint Bacillus subtilis spores on artificial meteorites survive hypervelocity atmospheric entry: implications for Lithopanspermia
    Patricia Fajardo-Cavazos
    Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, FL 32899, USA
    Astrobiology 5:726-36. 2005
    ....
  21. ncbi request reprint Spore UV and acceleration resistance of endolithic Bacillus pumilus and Bacillus subtilis isolates obtained from Sonoran desert basalt: implications for lithopanspermia
    James N Benardini
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona, USA
    Astrobiology 3:709-17. 2003
    ..subtilis, the benchmark organism, suggesting that spores of environmental B. pumilus isolates may be more likely to survive the rigors of interplanetary transfer...
  22. ncbi request reprint Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermia
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899, USA
    Astrobiology 5:536-44. 2005
    ..However, germination-induced bioluminescence was lower in spores exposed to simulated martian atmosphere, which suggests sublethal impairment of some endogenous spore germination processes...
  23. ncbi request reprint The solar UV environment and bacterial spore UV resistance: considerations for Earth-to-Mars transport by natural processes and human spaceflight
    Wayne L Nicholson
    Department of Microbiology and Cell Science, University of Florida, Mail Code UF 1, Building M6 1025 SLSL, Kennedy Space Center, FL 32899, USA
    Mutat Res 571:249-64. 2005
    ....
  24. pmc Novel rpoB mutations conferring rifampin resistance on Bacillus subtilis: global effects on growth, competence, sporulation, and germination
    Heather Maughan
    Departments of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
    J Bacteriol 186:2481-6. 2004
    ..The results thus establish the important role played by the RNA polymerase beta subunit, not only in the catalytic aspect of transcription, but also in the regulation of major developmental events in B. subtilis...
  25. pmc Persistence of biomarker ATP and ATP-generating capability in bacterial cells and spores contaminating spacecraft materials under earth conditions and in a simulated martian environment
    Patricia Fajardo-Cavazos
    Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, FL 32899, USA
    Appl Environ Microbiol 74:5159-67. 2008
    ....
  26. ncbi request reprint The paradox of the "ancient" bacterium which contains "modern" protein-coding genes
    Heather Maughan
    Mol Biol Evol 19:1637-9. 2002
  27. pmc Inactivation of vegetative cells, but not spores, of Bacillus anthracis, B. cereus, and B. subtilis on stainless steel surfaces coated with an antimicrobial silver- and zinc-containing zeolite formulation
    Belinda Galeano
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
    Appl Environ Microbiol 69:4329-31. 2003
    ....
  28. pmc Comparison of UV inactivation of spores of three encephalitozoon species with that of spores of two DNA repair-deficient Bacillus subtilis biodosimetry strains
    Marilyn M Marshall
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA
    Appl Environ Microbiol 69:683-5. 2003
    ..The results indicate that spores of Encephalitozoon spp. are readily inactivated at low UV fluences and that spores of UV-sensitive B. subtilis strains can be useful surrogates in evaluating UV reactor performance...
  29. ncbi request reprint Essential cysteine residues in Bacillus subtilis spore photoproduct lyase identified by alanine scanning mutagenesis
    Patricia Fajardo-Cavazos
    Department of Microbiology and Cell Science, University of Florida, Room 201 B Space Life Sciences Laboratory, Building M6 1025 SLSL, Kennedy Space Center, FL 32899, USA
    Curr Microbiol 51:331-5. 2005
    ....
  30. pmc Stochastic processes influence stationary-phase decisions in Bacillus subtilis
    Heather Maughan
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA
    J Bacteriol 186:2212-4. 2004
    ..In support of the noise hypothesis, we report that none of the populations responded to selection by improving their efficiency of sporulation, indicating that intracellular noise is independent of heritable genotype...