Research Topics
| Joseph D NgSummaryAffiliation: University of Alabama in Huntsville Country: USA Publications
| Collaborators
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Detail Information
Publications
PCR-based gene synthesis to produce recombinant proteins for crystallizationDamien Marsic
ExtremoZyme Inc, HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA
BMC Biotechnol 8:44. 2008..Existing methods, however, can be complex and not always reproducible, prompting researchers to use commercial suppliers rather than synthesize genes themselves...
New DNA polymerase from the hyperthermophilic marine archaeon Thermococcus thioreducensDamien Marsic
HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA
Extremophiles 12:775-88. 2008..TthiPolB performed remarkably well in a wide range of PCR conditions, being faster, more stable and more accurate than many commonly used enzymes...
Space-grown protein crystals are more useful for structure determinationJoseph D Ng
Laboratory for Structural Biology and the Department of Biological Sciences, University of Alabama in Huntsville, 35899, USA
Ann N Y Acad Sci 974:598-609. 2002..This lends evidence to the possibility that a microgravity environment can favor protein crystal growth and, subsequently, be more useful for structure determination...- Protein crystallization by capillary counterdiffusion for applied crystallographic structure determinationJoseph D Ng
Department of Biological Sciences and the Laboratory for Structural Biology, University of Alabama at Huntsville, Huntsville, AL 35899, USA
J Struct Biol 142:218-31. 2003..We review the historical progress of counterdiffusion crystallization, its application to X-ray crystallography, and ongoing tool development for high-throughput protein structure determination... - Inorganic pyrophosphatase crystals from Thermococcus thioreducens for X-ray and neutron diffractionRonny C Hughes
Department of Biological Sciences and Laboratory for Structural Biology, University of Alabama in Huntsville, Huntsville, AL 35899, USA
Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1482-7. 2012..1 Å resolution with I/σ(I) greater than 2.5. The preliminary crystallographic results reported here set in place future structure-function and mechanism studies of IPPase... - Expression, purification and preliminary X-ray analysis of proliferating cell nuclear antigen from the archaeon Thermococcus thioreducensMiranda L Byrne-Steele
Department of Biological Sciences, University of Alabama in Huntsville, Huntsville, AL 35899, USA
Acta Crystallogr Sect F Struct Biol Cryst Commun 65:906-9. 2009..The crystals belonged to the hexagonal space group P6(3), with unit-cell parameters a = b = 89.0, c = 62.8 A. Crystals of TtPCNA proved to be amenable to complete X-ray analysis and future structure determination... - Protein crystallization in restricted geometry: advancing old ideas for modern times in structural proteomicsJoseph D Ng
Hauptman Woodward Medical Research Institute, Buffalo, NY, USA
Methods Mol Biol 426:363-76. 2008..Applications and concepts presented here bring to light future prerequisites for the next generation of automation for structural genomics... - In situ X-ray analysis of protein crystals in low-birefringent and X-ray transmissive plastic microchannelsJoseph D Ng
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Acta Crystallogr D Biol Crystallogr 64:189-97. 2008..The results demonstrate the feasibility of rapid nanovolume counter-diffusion crystallization experiments without the need for additional instrumentation... - The high-throughput protein-to-structure pipeline at SECSGZhi Jie Liu
Southeast Collaboratory for Structural Genomics, Department of Biochechemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
Acta Crystallogr D Biol Crystallogr 61:679-84. 2005..Details of the various procedures in the pipeline for protein crystallization, target salvaging, data collection/processing and high-throughput structure determination/validation, as well as some examples, are described... - Life in the fast lane for protein crystallization and X-ray crystallographyMarc L Pusey
Biophysics SD48, NASA/MSFC Huntsville, AL 35812, USA
Prog Biophys Mol Biol 88:359-86. 2005..Examples of recent developments in this area are reported from the efforts of the Southeast Collaboratory for Structural Genomics (SECSG)... - Comparative analysis of space-grown and earth-grown crystals of an aminoacyl-tRNA synthetase: space-grown crystals are more useful for structural determinationJoseph D Ng
Département Mécanismes et Macromolécules de la Synthèse Protéique et Cristallogenèse, UPR 9002, Institut de Biologie Moleculaire et Cellulaire du CNRS, 15 rue Rene Descartes, F 67084 Strasbourg CEDEX, France
Acta Crystallogr D Biol Crystallogr 58:645-52. 2002..The major differences between the structures, including the better defined amino-acid side chains and the higher order of bound water molecules, are emphasized... - Ab initio crystallographic structure determination of insulin from protein to electron density without crystal handlingJose A Gavira
Laboratory for Structural Biology and the Department of Biological Sciences, University of Alabama in Huntsville, 35899, USA
Acta Crystallogr D Biol Crystallogr 58:1147-54. 2002..4 A resolution. This entire procedure from crystal growth to the determination of an initial structure was performed within four weeks...