Joseph D Ng

Summary

Affiliation: University of Alabama in Huntsville
Country: USA

Publications

  1. pmc PCR-based gene synthesis to produce recombinant proteins for crystallization
    Damien Marsic
    ExtremoZyme Inc, HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA
    BMC Biotechnol 8:44. 2008
  2. doi request reprint New DNA polymerase from the hyperthermophilic marine archaeon Thermococcus thioreducens
    Damien Marsic
    HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA
    Extremophiles 12:775-88. 2008
  3. ncbi request reprint Space-grown protein crystals are more useful for structure determination
    Joseph D Ng
    Laboratory for Structural Biology and the Department of Biological Sciences, University of Alabama in Huntsville, 35899, USA
    Ann N Y Acad Sci 974:598-609. 2002
  4. ncbi request reprint Protein crystallization by capillary counterdiffusion for applied crystallographic structure determination
    Joseph D Ng
    Department of Biological Sciences and the Laboratory for Structural Biology, University of Alabama at Huntsville, Huntsville, AL 35899, USA
    J Struct Biol 142:218-31. 2003
  5. pmc Inorganic pyrophosphatase crystals from Thermococcus thioreducens for X-ray and neutron diffraction
    Ronny C Hughes
    Department of Biological Sciences and Laboratory for Structural Biology, University of Alabama in Huntsville, Huntsville, AL 35899, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1482-7. 2012
  6. pmc Expression, purification and preliminary X-ray analysis of proliferating cell nuclear antigen from the archaeon Thermococcus thioreducens
    Miranda L Byrne-Steele
    Department of Biological Sciences, University of Alabama in Huntsville, Huntsville, AL 35899, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:906-9. 2009
  7. doi request reprint Protein crystallization in restricted geometry: advancing old ideas for modern times in structural proteomics
    Joseph D Ng
    Hauptman Woodward Medical Research Institute, Buffalo, NY, USA
    Methods Mol Biol 426:363-76. 2008
  8. doi request reprint In situ X-ray analysis of protein crystals in low-birefringent and X-ray transmissive plastic microchannels
    Joseph D Ng
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Acta Crystallogr D Biol Crystallogr 64:189-97. 2008
  9. ncbi request reprint The high-throughput protein-to-structure pipeline at SECSG
    Zhi Jie Liu
    Southeast Collaboratory for Structural Genomics, Department of Biochechemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Acta Crystallogr D Biol Crystallogr 61:679-84. 2005
  10. ncbi request reprint Life in the fast lane for protein crystallization and X-ray crystallography
    Marc L Pusey
    Biophysics SD48, NASA MSFC Huntsville, AL 35812, USA
    Prog Biophys Mol Biol 88:359-86. 2005

Collaborators

Detail Information

Publications12

  1. pmc PCR-based gene synthesis to produce recombinant proteins for crystallization
    Damien Marsic
    ExtremoZyme Inc, HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA
    BMC Biotechnol 8:44. 2008
    ..Existing methods, however, can be complex and not always reproducible, prompting researchers to use commercial suppliers rather than synthesize genes themselves...
  2. doi request reprint New DNA polymerase from the hyperthermophilic marine archaeon Thermococcus thioreducens
    Damien Marsic
    HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA
    Extremophiles 12:775-88. 2008
    ..TthiPolB performed remarkably well in a wide range of PCR conditions, being faster, more stable and more accurate than many commonly used enzymes...
  3. ncbi request reprint Space-grown protein crystals are more useful for structure determination
    Joseph D Ng
    Laboratory for Structural Biology and the Department of Biological Sciences, University of Alabama in Huntsville, 35899, USA
    Ann N Y Acad Sci 974:598-609. 2002
    ..This lends evidence to the possibility that a microgravity environment can favor protein crystal growth and, subsequently, be more useful for structure determination...
  4. ncbi request reprint Protein crystallization by capillary counterdiffusion for applied crystallographic structure determination
    Joseph D Ng
    Department of Biological Sciences and the Laboratory for Structural Biology, University of Alabama at Huntsville, Huntsville, AL 35899, USA
    J Struct Biol 142:218-31. 2003
    ..We review the historical progress of counterdiffusion crystallization, its application to X-ray crystallography, and ongoing tool development for high-throughput protein structure determination...
  5. pmc Inorganic pyrophosphatase crystals from Thermococcus thioreducens for X-ray and neutron diffraction
    Ronny C Hughes
    Department of Biological Sciences and Laboratory for Structural Biology, University of Alabama in Huntsville, Huntsville, AL 35899, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1482-7. 2012
    ..1 Å resolution with I/σ(I) greater than 2.5. The preliminary crystallographic results reported here set in place future structure-function and mechanism studies of IPPase...
  6. pmc Expression, purification and preliminary X-ray analysis of proliferating cell nuclear antigen from the archaeon Thermococcus thioreducens
    Miranda L Byrne-Steele
    Department of Biological Sciences, University of Alabama in Huntsville, Huntsville, AL 35899, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:906-9. 2009
    ..The crystals belonged to the hexagonal space group P6(3), with unit-cell parameters a = b = 89.0, c = 62.8 A. Crystals of TtPCNA proved to be amenable to complete X-ray analysis and future structure determination...
  7. doi request reprint Protein crystallization in restricted geometry: advancing old ideas for modern times in structural proteomics
    Joseph D Ng
    Hauptman Woodward Medical Research Institute, Buffalo, NY, USA
    Methods Mol Biol 426:363-76. 2008
    ..Applications and concepts presented here bring to light future prerequisites for the next generation of automation for structural genomics...
  8. doi request reprint In situ X-ray analysis of protein crystals in low-birefringent and X-ray transmissive plastic microchannels
    Joseph D Ng
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Acta Crystallogr D Biol Crystallogr 64:189-97. 2008
    ..The results demonstrate the feasibility of rapid nanovolume counter-diffusion crystallization experiments without the need for additional instrumentation...
  9. ncbi request reprint The high-throughput protein-to-structure pipeline at SECSG
    Zhi Jie Liu
    Southeast Collaboratory for Structural Genomics, Department of Biochechemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Acta Crystallogr D Biol Crystallogr 61:679-84. 2005
    ..Details of the various procedures in the pipeline for protein crystallization, target salvaging, data collection/processing and high-throughput structure determination/validation, as well as some examples, are described...
  10. ncbi request reprint Life in the fast lane for protein crystallization and X-ray crystallography
    Marc L Pusey
    Biophysics SD48, NASA MSFC Huntsville, AL 35812, USA
    Prog Biophys Mol Biol 88:359-86. 2005
    ..Examples of recent developments in this area are reported from the efforts of the Southeast Collaboratory for Structural Genomics (SECSG)...
  11. ncbi request reprint Comparative analysis of space-grown and earth-grown crystals of an aminoacyl-tRNA synthetase: space-grown crystals are more useful for structural determination
    Joseph D Ng
    Département Mécanismes et Macromolécules de la Synthèse Protéique et Cristallogenèse, UPR 9002, Institut de Biologie Moleculaire et Cellulaire du CNRS, 15 rue Rene Descartes, F 67084 Strasbourg CEDEX, France
    Acta Crystallogr D Biol Crystallogr 58:645-52. 2002
    ..The major differences between the structures, including the better defined amino-acid side chains and the higher order of bound water molecules, are emphasized...
  12. ncbi request reprint Ab initio crystallographic structure determination of insulin from protein to electron density without crystal handling
    Jose A Gavira
    Laboratory for Structural Biology and the Department of Biological Sciences, University of Alabama in Huntsville, 35899, USA
    Acta Crystallogr D Biol Crystallogr 58:1147-54. 2002
    ..4 A resolution. This entire procedure from crystal growth to the determination of an initial structure was performed within four weeks...