Gary Nelsestuen

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. ncbi Top-down proteomic analysis by MALDI-TOF profiling: Concentration-independent biomarkers
    Gary L Nelsestuen
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA
    Proteomics Clin Appl 2:158-66. 2008
  2. ncbi Plasma protein profiling: unique and stable features of individuals
    Gary L Nelsestuen
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Proteomics 5:4012-24. 2005
  3. ncbi Proteomic identification of human neutrophil alpha-defensins in chronic lung allograft rejection
    Gary L Nelsestuen
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA
    Proteomics 5:1705-13. 2005
  4. ncbi Views on methods for monitoring recombinant factor VIIa in inhibitor patients
    Nigel S Key
    Department of Medicine, Divisionof Hematology, University of Minnesota, MN, USA
    Semin Hematol 41:51-4. 2004
  5. ncbi Analysis of chronic lung transplant rejection by MALDI-TOF profiles of bronchoalveolar lavage fluid
    Yan Zhang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, MN 55455, USA
    Proteomics 6:1001-10. 2006
  6. ncbi Studying multiple protein profiles over time to assess biomarker validity
    Raj S Kasthuri
    University of Minnesota, Department of Medicine, MMC 480, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Expert Rev Proteomics 3:455-64. 2006
  7. ncbi Possible synergy between recombinant factor VIIa and prothrombin complex concentrate in hemophilia therapy
    Nigel S Key
    Department of Medicine, University of Minnesota, Minneapolis, USA
    Thromb Haemost 88:60-5. 2002
  8. ncbi Mutagenesis of the gamma-carboxyglutamic acid domain of human factor VII to generate maximum enhancement of the membrane contact site
    Stephen B Harvey
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis 55455, USA
    J Biol Chem 278:8363-9. 2003
  9. ncbi Unusual benefits of macromolecular shielding by polyethylene glycol for reactions at the diffusional limit: the case of factor VIIai and tissue factor
    Matthew Stone
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, MN 55108, USA
    Biochemistry 41:15820-5. 2002
  10. ncbi Large enhancement of functional activity of active site-inhibited factor VIIa due to protein dimerization: insights into mechanism of assembly/disassembly from tissue factor
    Matthew D Stone
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 6 155 Jackson Hall, 321 Church Street SE, Minneapolis, Minnesota 55455, USA
    Biochemistry 44:6321-30. 2005

Research Grants

  1. QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETER
    Gary Nelsestuen; Fiscal Year: 2001
  2. ENHANCED VITAMIN K DEPENDENT PROTEINS
    Gary Nelsestuen; Fiscal Year: 2002

Collaborators

Detail Information

Publications11

  1. ncbi Top-down proteomic analysis by MALDI-TOF profiling: Concentration-independent biomarkers
    Gary L Nelsestuen
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA
    Proteomics Clin Appl 2:158-66. 2008
    ..Diagnosis by longitudinal analysis of the same individual might increase sensitivity of protein biomarkers by 20-fold or more. Protein changes that exceed the range of values found among healthy individuals may be uncommon...
  2. ncbi Plasma protein profiling: unique and stable features of individuals
    Gary L Nelsestuen
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Proteomics 5:4012-24. 2005
    ....
  3. ncbi Proteomic identification of human neutrophil alpha-defensins in chronic lung allograft rejection
    Gary L Nelsestuen
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA
    Proteomics 5:1705-13. 2005
    ..HNP levels did not correlate with episodes of acute rejection, cytomegalovirus or fungal infection. In conclusion, elevated HNP levels are associated with the onset of BOS and can predate the clinical onset of disease up to 15 months...
  4. ncbi Views on methods for monitoring recombinant factor VIIa in inhibitor patients
    Nigel S Key
    Department of Medicine, Divisionof Hematology, University of Minnesota, MN, USA
    Semin Hematol 41:51-4. 2004
    ..To date, however, no assay endpoint has been developed that can correlate accurately with clinical outcomes in a sufficiently large patient sample...
  5. ncbi Analysis of chronic lung transplant rejection by MALDI-TOF profiles of bronchoalveolar lavage fluid
    Yan Zhang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, MN 55455, USA
    Proteomics 6:1001-10. 2006
    ..Overall, this study demonstrated the feasibility of this approach for the detection of disease biomarkers...
  6. ncbi Studying multiple protein profiles over time to assess biomarker validity
    Raj S Kasthuri
    University of Minnesota, Department of Medicine, MMC 480, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Expert Rev Proteomics 3:455-64. 2006
    ..The focus of this review is to illustrate the principle and value of serial protein profiling using a rapid protein extraction method...
  7. ncbi Possible synergy between recombinant factor VIIa and prothrombin complex concentrate in hemophilia therapy
    Nigel S Key
    Department of Medicine, University of Minnesota, Minneapolis, USA
    Thromb Haemost 88:60-5. 2002
    ..Results with other patients supported this hypothesis. With further evaluation, a therapeutic approach combining sequential PCC and rFVIIa may prove useful for treatment of bleeding refractory to either agent used alone...
  8. ncbi Mutagenesis of the gamma-carboxyglutamic acid domain of human factor VII to generate maximum enhancement of the membrane contact site
    Stephen B Harvey
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis 55455, USA
    J Biol Chem 278:8363-9. 2003
    ..Overall, the results suggest features of membrane binding by vitamin K-dependent proteins and provide reagents that may prove useful for research and therapy...
  9. ncbi Unusual benefits of macromolecular shielding by polyethylene glycol for reactions at the diffusional limit: the case of factor VIIai and tissue factor
    Matthew Stone
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, MN 55108, USA
    Biochemistry 41:15820-5. 2002
    ..Overall, properties of PEG-modified proteins can suggest features of the kinetic mechanism and may provide enhanced proteins for anticoagulation therapy...
  10. ncbi Large enhancement of functional activity of active site-inhibited factor VIIa due to protein dimerization: insights into mechanism of assembly/disassembly from tissue factor
    Matthew D Stone
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 6 155 Jackson Hall, 321 Church Street SE, Minneapolis, Minnesota 55455, USA
    Biochemistry 44:6321-30. 2005
    ..These results suggest novel actions of fVIIai that distinguish it from most of the anticoagulants that block later steps of the coagulation cascade...
  11. ncbi Response of factor VIII and IX-deficient blood to wild type and high membrane affinity mutant factor VIIa in an in vitro whole blood clotting assay: possible correlation to clinical outcome
    Nicole Henderson
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St Paul 55108, USA
    Thromb Haemost 88:98-103. 2002
    ..The results may justify larger studies to determine the utility of this assay for evaluation of patient responsiveness and to set factor VIIa therapy levels...

Research Grants7

  1. QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETER
    Gary Nelsestuen; Fiscal Year: 2001
    ..An accessory nanospray HPLC system for application of small samples is also requested. The instrumentation that is requested is also critical for the emerging area of non-covalent macromolecular complexes. ..
  2. ENHANCED VITAMIN K DEPENDENT PROTEINS
    Gary Nelsestuen; Fiscal Year: 2002
    ..It will increase knowledge of the role that membranes play in enzymatic events of coagulation and in biological systems. It may ultimately provide new and/or improved materials for biomedical applications. ..