James Nataro

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. ncbi request reprint Enteroaggregative Escherichia coli pathogenesis
    James P Nataro
    University of Maryland, School of Medicine, Baltimore, MD, USA
    Curr Opin Gastroenterol 21:4-8. 2005
  2. ncbi request reprint Vaccines against diarrheal diseases
    James P Nataro
    University of Maryland, School of Medicine, Center for Vaccine Development, 685 W Baltimore St, Baltimore, MD 21201, USA
    Semin Pediatr Infect Dis 15:272-9. 2004
  3. pmc Atypical enteropathogenic Escherichia coli: typical pathogens?
    James P Nataro
    Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Emerg Infect Dis 12:696. 2006
  4. ncbi request reprint Diarrheagenic Escherichia coli infection in Baltimore, Maryland, and New Haven, Connecticut
    James P Nataro
    Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Clin Infect Dis 43:402-7. 2006
  5. pmc The Pic protease of enteroaggregative Escherichia coli promotes intestinal colonization and growth in the presence of mucin
    Susan M Harrington
    Center for Vaccine Development, University of Maryland, Baltimore, MD 21201, USA
    Infect Immun 77:2465-73. 2009
  6. ncbi request reprint Pathogenesis of enteroaggregative Escherichia coli infection
    Susan M Harrington
    Department of Pediatrics, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, USA
    FEMS Microbiol Lett 254:12-8. 2006
  7. doi request reprint Autoactivation of the AggR regulator of enteroaggregative Escherichia coli in vitro and in vivo
    Nicholas Morin
    Department of Pediatrics and Microbiology and Immunology, Center for Vaccine Development, University of Maryland, Baltimore, MD 21201, USA
    FEMS Immunol Med Microbiol 58:344-55. 2010
  8. pmc Regulation of the overlapping pic/set locus in Shigella flexneri and enteroaggregative Escherichia coli
    Martin Behrens
    Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 70:2915-25. 2002
  9. ncbi request reprint Structure-function analysis of the enteroaggregative Escherichia coli plasmid-encoded toxin autotransporter using scanning linker mutagenesis
    Pinaki R Dutta
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 278:39912-20. 2003
  10. doi request reprint Isolation, identification, and characterization of small bioactive peptides from Lactobacillus GG conditional media that exert both anti-Gram-negative and Gram-positive bactericidal activity
    Ruiliang Lu
    University of Maryland School of Medicine, Mucosal Biology Research Center, Health Science Facility II, 20 Penn St, Baltimore, MD 21201, USA
    J Pediatr Gastroenterol Nutr 49:23-30. 2009

Research Grants

  1. Dispersin from Enteroaggregative E. coli
    James Nataro; Fiscal Year: 2004
  2. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 2004
  3. Virulence in Enteroaggregative Escherichia coli
    James Nataro; Fiscal Year: 2005
  4. Virulence in Enteroaggregative Escherichia coli
    James Nataro; Fiscal Year: 2006
  5. Virulence in Enteroaggregative Escherichia coli
    James Nataro; Fiscal Year: 2007
  6. Live Vector Vaccines Against Agents of Bioterror
    James Nataro; Fiscal Year: 2007
  7. Virulence in Enteroaggregative Escherichia coli
    James Nataro; Fiscal Year: 2009
  8. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 2003
  9. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 1999
  10. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 2000

Detail Information

Publications58

  1. ncbi request reprint Enteroaggregative Escherichia coli pathogenesis
    James P Nataro
    University of Maryland, School of Medicine, Baltimore, MD, USA
    Curr Opin Gastroenterol 21:4-8. 2005
    ..Understanding of EAEC pathogenesis is rapidly increasing, and advances are proving relevant to epidemiologic and clinical investigations...
  2. ncbi request reprint Vaccines against diarrheal diseases
    James P Nataro
    University of Maryland, School of Medicine, Center for Vaccine Development, 685 W Baltimore St, Baltimore, MD 21201, USA
    Semin Pediatr Infect Dis 15:272-9. 2004
    ..For this reason, development of vaccines against the most important gastrointestinal infections remains a high priority...
  3. pmc Atypical enteropathogenic Escherichia coli: typical pathogens?
    James P Nataro
    Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Emerg Infect Dis 12:696. 2006
  4. ncbi request reprint Diarrheagenic Escherichia coli infection in Baltimore, Maryland, and New Haven, Connecticut
    James P Nataro
    Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Clin Infect Dis 43:402-7. 2006
    ..Diarrhea remains a common complaint among US patients who seek medical attention...
  5. pmc The Pic protease of enteroaggregative Escherichia coli promotes intestinal colonization and growth in the presence of mucin
    Susan M Harrington
    Center for Vaccine Development, University of Maryland, Baltimore, MD 21201, USA
    Infect Immun 77:2465-73. 2009
    ..The data suggest a novel metabolic role for the Pic mucinase in EAEC colonization...
  6. ncbi request reprint Pathogenesis of enteroaggregative Escherichia coli infection
    Susan M Harrington
    Department of Pediatrics, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, USA
    FEMS Microbiol Lett 254:12-8. 2006
    ..This article reviews these virulence elements and presents on-going areas of research in EAEC pathogenesis...
  7. doi request reprint Autoactivation of the AggR regulator of enteroaggregative Escherichia coli in vitro and in vivo
    Nicholas Morin
    Department of Pediatrics and Microbiology and Immunology, Center for Vaccine Development, University of Maryland, Baltimore, MD 21201, USA
    FEMS Immunol Med Microbiol 58:344-55. 2010
    ..The data provide significant insights into the regulation cascade leading to aggR expression in the mammalian intestine during EAEC infection...
  8. pmc Regulation of the overlapping pic/set locus in Shigella flexneri and enteroaggregative Escherichia coli
    Martin Behrens
    Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 70:2915-25. 2002
    ..Our studies provide the first insights into the mechanisms affecting expression of this unusual virulence locus...
  9. ncbi request reprint Structure-function analysis of the enteroaggregative Escherichia coli plasmid-encoded toxin autotransporter using scanning linker mutagenesis
    Pinaki R Dutta
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 278:39912-20. 2003
    ..Our data suggest the existence of separate functional domains mediating Pet proteolysis, secretion, and cell interaction...
  10. doi request reprint Isolation, identification, and characterization of small bioactive peptides from Lactobacillus GG conditional media that exert both anti-Gram-negative and Gram-positive bactericidal activity
    Ruiliang Lu
    University of Maryland School of Medicine, Mucosal Biology Research Center, Health Science Facility II, 20 Penn St, Baltimore, MD 21201, USA
    J Pediatr Gastroenterol Nutr 49:23-30. 2009
    ..Therefore, the main objective of this work was to identify and characterize moieties elaborated by LGG that exert antibacterial activity...
  11. ncbi request reprint EilA, a HilA-like regulator in enteroaggregative Escherichia coli
    Jalaluddin Sheikh
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Microbiol 61:338-50. 2006
    ..Contributions of this new putative virulence-related regulon in EAEC may include adherence, aggregation, and as yet uncharacterized roles for the T3SS...
  12. pmc Escherichia coli O157:H7 infection in Dutch belted and New Zealand white rabbits
    Aruna Panda
    Department of Pathology, University of Maryland School of Medicine, Baltimore, USA
    Comp Med 60:31-7. 2010
    ..Our findings provide evidence that both breeds are susceptible to E. coli O157:H7 infection and that both may be useful models for investigating EHEC infections of humans...
  13. pmc The role of patient-to-patient transmission in the acquisition of imipenem-resistant Pseudomonas aeruginosa colonization in the intensive care unit
    J Kristie Johnson
    Department of Pathology, Institute of Genomic Sciences, University of Maryland School of Medicine, 10 South Pine Street, Baltimore, MD 21201, USA
    J Infect Dis 200:900-5. 2009
    ..Imipenem-resistant Pseudomonas aeruginosa (IRPA) is an emerging problem. The causal role of antibiotic selective pressure versus patient-to-patient transmission has not been assessed using a large cohort...
  14. ncbi request reprint Deletion in the Shigella enterotoxin genes further attenuates Shigella flexneri 2a bearing guanine auxotrophy in a phase 1 trial of CVD 1204 and CVD 1208
    Karen L Kotloff
    Division of Infectious Disease and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Infect Dis 190:1745-54. 2004
    ....
  15. ncbi request reprint Hydrophobic residues of the autotransporter EspP linker domain are important for outer membrane translocation of its passenger
    Jorge J Velarde
    Center for Vaccine Development, Department of Biochemistry, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 279:31495-504. 2004
    ..Moreover, amino acids of hydrophobic character play a crucial role in linker function, suggesting the existence of a hydrophobic core or hydrophobic interaction necessary for outer membrane translocation of autotransporter proteins...
  16. ncbi request reprint Intestinal epithelial tight junctions as targets for enteric bacteria-derived toxins
    Alessio Fasano
    Division of Pediatric Gastroenterology and Nutrition, School of Medicine, University of Maryland, Baltimore 21201, USA
    Adv Drug Deliv Rev 56:795-807. 2004
    ..Many pathogenic bacteria exploit this system to accomplish their pathogenic strategies by ultimately modulating intestinal permeability...
  17. ncbi request reprint Safety and immunogenicity of CVD 1208S, a live, oral DeltaguaBA Deltasen Deltaset Shigella flexneri 2a vaccine grown on animal-free media
    Karen L Kotloff
    Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Hum Vaccin 3:268-75. 2007
    ....
  18. ncbi request reprint Solution structure of the novel dispersin protein of enteroaggregative Escherichia coli
    Jorge J Velarde
    Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Mol Microbiol 66:1123-35. 2007
    ..We also show that the AAF organelles contribute positive charge to the bacterial surface, suggesting that dispersin's role in fimbrial function is to overcome electrostatic attraction between AAF and the bacterial surface...
  19. pmc Roles of periplasmic chaperone proteins in the biogenesis of serine protease autotransporters of Enterobacteriaceae
    Fernando Ruiz-Perez
    Center for Vaccine Development, University ofMaryland School of Medicine, Baltimore, Maryland 21201, USA
    J Bacteriol 191:6571-83. 2009
    ..Our data suggest that translocation of AT proteins may require accessory factors, calling into question the moniker "autotransporter."..
  20. ncbi request reprint The export of coat protein from enteroaggregative Escherichia coli by a specific ATP-binding cassette transporter system
    Junichiro Nishi
    Center for Vaccine Development, Departments of Pediatrics, Medicine, and Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 278:45680-9. 2003
    ..We propose that the aat cluster encodes a specialized ABC transporter, which plays a role in the pathogenesis of EAEC by transporting dispersin out of the bacterial cell...
  21. pmc Mucosally delivered Salmonella typhi expressing the Yersinia pestis F1 antigen elicits mucosal and systemic immunity early in life and primes the neonatal immune system for a vigorous anamnestic response to parenteral F1 boost
    Karina Ramirez
    Center for Vaccine Development, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Immunol 182:1211-22. 2009
    ..Such a strategy can prevent infectious diseases, including those considered biowarfare threats, early in life...
  22. ncbi request reprint Pathogenic Escherichia coli
    James B Kaper
    Center for Vaccine Development, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Nat Rev Microbiol 2:123-40. 2004
    ..Several different E. coli strains cause diverse intestinal and extraintestinal diseases by means of virulence factors that affect a wide range of cellular processes...
  23. pmc Phase I evaluation of delta virG Shigella sonnei live, attenuated, oral vaccine strain WRSS1 in healthy adults
    Karen L Kotloff
    Division of Infectious Disease and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 70:2016-21. 2002
    ..We conclude that WRSS1 vaccine is remarkably immunogenic in doses ranging from 10(3) to 10(6) CFU but elicits clinical reactions that must be assessed in further volunteer trials...
  24. pmc The major pilin subunit of the AAF/II fimbriae from enteroaggregative Escherichia coli mediates binding to extracellular matrix proteins
    Mauricio J Farfan
    Department of Pediatrics, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Infect Immun 76:4378-84. 2008
    ..Finally, increased bacterial adherence was observed when apical secretion of fibronectin was induced by adenosine in polarized T84 cells. Binding to fibronectin may contribute to colonization of the gastrointestinal tract by EAEC...
  25. ncbi request reprint Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli
    Edward G Dudley
    Center for Vaccine Development, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD 21201, USA
    Mol Microbiol 61:1267-82. 2006
    ..These data support the hypothesis that AggR is a global regulator of EAEC virulence determinants, and builds on the hypothesis that T6SS is an importance mediator of pathogenesis...
  26. pmc Functional comparison of serine protease autotransporters of enterobacteriaceae
    Pinaki R Dutta
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore 21201, USA
    Infect Immun 70:7105-13. 2002
    ..Our data suggest that, despite the homologies observed among these proteins, the SPATEs have different pathogenetic functions only partly dependent on their substrate specificities...
  27. pmc Identification by PCR of non-typhoidal Salmonella enterica serovars associated with invasive infections among febrile patients in Mali
    Sharon M Tennant
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, USA
    PLoS Negl Trop Dis 4:e621. 2010
    ..Importantly, including data from Mali, three serovars, Salmonella enterica serovar Typhimurium, Salmonella Enteritidis and Salmonella Dublin, account for the majority of non-typhoidal Salmonella isolated from these patients...
  28. pmc An IncI1 plasmid contributes to the adherence of the atypical enteroaggregative Escherichia coli strain C1096 to cultured cells and abiotic surfaces
    Edward G Dudley
    Center for Vaccine Development, University of Maryland, 685 W Baltimore St, Baltimore, MD 21201, USA
    Infect Immun 74:2102-14. 2006
    ..We also observe that AA can be mediated by factors distinct from AAF adhesins...
  29. pmc Intracellular expression of the plasmid-encoded toxin from enteroaggregative Escherichia coli
    Bao Quan Sui
    Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 71:5364-70. 2003
    ..These studies corroborate an intracellular site of action for the Pet toxin, further implicate a role for spectrin in Pet intoxication, and provide a powerful tool for Pet structure and function analyses...
  30. ncbi request reprint Cryptosporidiosis stimulates an inflammatory intestinal response in malnourished Haitian children
    Beth D Kirkpatrick
    Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    J Infect Dis 186:94-101. 2002
    ..01). A Th1 cytokine response to infection was not detected. This triple cohort study demonstrates that malnourished children with acute cryptosporidiosis mount inflammatory, Th-2, and counterregulatory intestinal immune responses...
  31. pmc A challenge model for Shigella dysenteriae 1 in cynomolgus monkeys (Macaca fascicularis)
    Steven T Shipley
    Department of Pathology, University of Maryland School of Medicine, Baltimore, USA
    Comp Med 60:54-61. 2010
    ..We believe that this animal model may provide unique insights into the immunologic mechanisms of protection to S. dysenteriae 1 infection and in advancing development of a vaccine against shigellosis...
  32. pmc A novel dispersin protein in enteroaggregative Escherichia coli
    Jalaluddin Sheikh
    Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA
    J Clin Invest 110:1329-37. 2002
    ..Since dispersin is expressed in vivo, is highly immunogenic, and is present in most EAEC strains, it holds considerable promise as an EAEC immunogen...
  33. pmc PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever
    Haim Levy
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Clin Microbiol 46:1861-6. 2008
    ..Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity...
  34. ncbi request reprint Aggregative adherence fimbriae contribute to the inflammatory response of epithelial cells infected with enteroaggregative Escherichia coli
    Susan M Harrington
    Department of Microbiology, The University of Maryland, Baltimore, MD 21201, USA
    Cell Microbiol 7:1565-78. 2005
    ..Our data suggest that multiple factors contribute to EAEC-induced inflammation, and further characterization of the nature of EAEC proinflammatory factors will greatly advance our understanding of this emerging pathogen...
  35. ncbi request reprint Prevention of enteric diseases
    Ina Stephens
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, USA
    Adv Exp Med Biol 549:71-82. 2004
    ..There has been much success in this arena, but much more needs to be done. Solutions will depend on new and old technologies and on continued dedication of human and financial resources to address problems of global significance...
  36. pmc Use of a continuous-flow anaerobic culture to characterize enteric virulence gene expression
    Fernando Ruiz-Perez
    Center for Vaccine Development, University of Maryland School of Medicine, 685 W Baltimore Street, Baltimore, MD 21201, USA
    Infect Immun 72:3793-802. 2004
    ..Our data provide new insights into regulation of virulence genes in EAEC and suggest the utility of intestinal simulation cultures in characterizing enteric gene regulation...
  37. pmc An in vivo expression technology screen for Vibrio cholerae genes expressed in human volunteers
    Mary Jane Lombardo
    Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 104:18229-34. 2007
    ..VC0201 (fhuC), which encodes the ATPase of a ferrichrome ABC transporter, is one of the identified in vivo-induced genes and is required for virulence in the mouse model...
  38. pmc Bacteria-host communication: the language of hormones
    Vanessa Sperandio
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    Proc Natl Acad Sci U S A 100:8951-6. 2003
    ..Given that eukaryotic cell-to-cell signaling typically occurs through hormones, and that bacterial cell-to-cell signaling occurs through QS, we speculate that QS might be a "language" by which bacteria and host cells communicate...
  39. pmc Rate of occurrence and pathogenic effect of enteroaggregative Escherichia coli virulence factors in international travelers
    Zhi Dong Jiang
    The University of Texas Health Science Center School of Public Health, Houston, Texas 77030, USA
    J Clin Microbiol 40:4185-90. 2002
    ..The different patterns of virulence factors of EAEC revealed several clusters demonstrating diversity among the isolates from the various regions...
  40. doi request reprint The Escherichia coli biofilm-promoting protein Antigen 43 does not contribute to intestinal colonization
    Maria das Graças de Luna
    Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham, UK
    FEMS Microbiol Lett 284:237-46. 2008
    ..Unexpectedly, considering its similarity to other adhesins, our findings suggest that Ag43 does not act as a direct colonization factor by binding to mammalian cells...
  41. pmc New adhesin of enteroaggregative Escherichia coli related to the Afa/Dr/AAF family
    Nadia Boisen
    Statens Serum Institut, Department of Bacteriology, Mycology and Parasitology, Copenhagen, Denmark
    Infect Immun 76:3281-92. 2008
    ..Four related adhesins have now been found to confer AA in typical EAEC strains; our data suggest that, together, these variants may account for AA in the large majority of strains...
  42. pmc Detection of enteroaggregative Escherichia coli with formalin-preserved HEp-2 cells
    Mohamad S Miqdady
    Baylor College of Medicine, Houston, Texas, USA
    J Clin Microbiol 40:3066-7. 2002
    ..Using formalin-fixed HEp-2 cells will improve the efficiency of EAEC identification...
  43. doi request reprint Seroprevalence of the enteroaggregative Escherichia coli virulence factor dispersin among USA travellers to Cuernavaca, Mexico: a pilot study
    David B Huang
    Department of Medicine, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    J Med Microbiol 57:476-9. 2008
    ..Further studies are needed to characterize in more detail the host clinical and immunological responses to the dispersin protein...
  44. ncbi request reprint Role of type I fimbriae in the aggregative adhesion pattern of enteroaggregative Escherichia coli
    Cristiano G Moreira
    Laboratório Especial de Microbiologia, Instituto Butantan, Avenida Vital Brazil 1500, 05503 900, SP, Sao Paulo, Brazil
    FEMS Microbiol Lett 226:79-85. 2003
    ..Our data evidence a role for TIF in the AA pattern and in EAEC biofilm formation, demonstrating that these phenotypes are multifactorial...
  45. pmc Typical enteroaggregative Escherichia coli is the most prevalent pathotype among E. coli strains causing diarrhea in Mongolian children
    Jav Sarantuya
    Department of Pediatrics, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
    J Clin Microbiol 42:133-9. 2004
    ..Genetic analysis showed the heterogeneity of EAEC but illustrated the importance of the AggR regulon (denoting typical EAEC) as a marker for virulent EAEC strains...
  46. ncbi request reprint [Validation of a PCR technique for the detection of enteroagreggative Escherichia coli causing traveler's diarrhea]
    Joaquim Ruiz-Blázquez
    Centro de Salud Internacional, IDIBAPS, Hospital Clinic, Barcelona, Spain
    Enferm Infecc Microbiol Clin 23:479-81. 2005
    ..Detection of enteroaggregative strains of Escherichia coli (EAEC) by the gold standard technique (cellular adherence) is difficult to implement in routine clinical practice...
  47. ncbi request reprint Quantitative biofilm assay using a microtiter plate to screen for enteroaggregative Escherichia coli
    Naoko Wakimoto
    Department of Pediatrics, Kagoshima University Graduate School of Medical and Dental Sciences, 8 35 1 Sakuragaoka, Kagoshima 890 8520, Japan
    Am J Trop Med Hyg 71:687-90. 2004
    ..2. Twenty-one aggR+ and 27 aggR - EAEC strains could be screened by an OD(570) > 0.2 using this assay. Although confirmation by a HEp-2 cell adherence test is needed, this biofilm assay is convenient and useful in screening for EAEC...
  48. ncbi request reprint Acute, infectious diarrhea among children in developing countries
    Laura Jean Podewils
    Respiratory and Enteric Viruses Branch, Division of Viral and Ricksettial Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA
    Semin Pediatr Infect Dis 15:155-68. 2004
  49. pmc The serine protease motif of EspC from enteropathogenic Escherichia coli produces epithelial damage by a mechanism different from that of Pet toxin from enteroaggregative E. coli
    Fernando Navarro-Garcia
    Department of Cell Biology, CINVESTAV IPN, 07000 Mexico, DF, Mexico
    Infect Immun 72:3609-21. 2004
    ..However, these differences may confer a specialized role on EspC in the pathogenesis of EPEC, which is different from that of Pet in EAEC pathogenesis...
  50. ncbi request reprint Prevalence of diarrheagenic Escherichia coli in acute childhood enteritis: a prospective controlled study
    Mitchell B Cohen
    Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    J Pediatr 146:54-61. 2005
    ..Since diarrheagenic E. coli are not identified by common clinical laboratory techniques, we hypothesized that these organisms might be an unrecognized cause of enteritis in children in the U.S...
  51. pmc Association of putative enteroaggregative Escherichia coli virulence genes and biofilm production in isolates from travelers to developing countries
    Jamal A Mohamed
    Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, TX 77030, USA
    J Clin Microbiol 45:121-6. 2007
    ..This study suggests that biofilm formation is a common phenomenon among EAEC isolates derived from travelers with or without diarrhea and that multiple genes associated with biofilm formation are regulated by aggR...
  52. pmc Oral administration of a Salmonella enterica-based vaccine expressing Bacillus anthracis protective antigen confers protection against aerosolized B. anthracis
    Margaret G M Stokes
    Department of Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom
    Infect Immun 75:1827-34. 2007
    ..Thus, we demonstrate for the first time the efficacy of an oral S. enterica-based vaccine against aerosolized B. anthracis spores...
  53. ncbi request reprint A conserved extended signal peptide region directs posttranslational protein translocation via a novel mechanism
    Mickael Desvaux
    Division of Immunity and Infection, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Microbiology 153:59-70. 2007
    ..Mutational analysis suggests that the ESPR delays inner membrane translocation by adopting a particular conformation, or by interacting with a cytoplasmic or inner membrane co-factor, prior to inner membrane translocation...
  54. ncbi request reprint Virulence characteristics and the molecular epidemiology of enteroaggregative Escherichia coli isolates from travellers to developing countries
    David B Huang
    Baylor College of Medicine, Division of Infectious Diseases, Houston, TX, USA
    J Med Microbiol 56:1386-92. 2007
    ....
  55. pmc Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains
    Jorge F Cerna
    Department of Molecular Biomedicine, CINVESTAV IPN, Zacatenco, Mexico DF 07360, Mexico
    J Clin Microbiol 41:2138-40. 2003
    ..One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC...
  56. ncbi request reprint Recombinant Salmonella vaccines for biodefence
    Helen S Atkins
    Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK
    Vaccine 24:2710-7. 2006
    ..In this review, the technologies used, considerations needed, progress made, and future prospects for developing multivalent Salmonella-based vaccines for biodefence are discussed...
  57. ncbi request reprint Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli
    Claire Jenkins
    Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK
    FEMS Microbiol Lett 253:119-24. 2005
    ..Our data suggest that strains previously designated as typical EAEC harbour a large number of conserved plasmid and chromosomal loci, further illuminating a package of virulence genes common to the most important EAEC...
  58. ncbi request reprint Enteroaggregative Escherichia coli is a cause of acute diarrheal illness: a meta-analysis
    David B Huang
    Division of Infectious Diseases, Baylor College of Medicine, University of Texas at Houston, USA
    Clin Infect Dis 43:556-63. 2006
    ..The objective of this meta-analysis was to determine whether identification of EAEC in stool samples is associated with acute diarrheal illness among different subpopulations, by geographic area...

Research Grants24

  1. Dispersin from Enteroaggregative E. coli
    James Nataro; Fiscal Year: 2004
    ..Each protein will be localized and their roles addressed using mutagenesis and biochemical strategies. ..
  2. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 2004
    ..abstract_text> ..
  3. Virulence in Enteroaggregative Escherichia coli
    James Nataro; Fiscal Year: 2005
    ..In this aim, we will take a genomics approach to look for additional AggR-regulated genes, and characterize expression of the AggR regulon in vivo. ..
  4. Virulence in Enteroaggregative Escherichia coli
    James Nataro; Fiscal Year: 2006
    ..In this aim, we will take a genomics approach to look for additional AggR-regulated genes, and characterize expression of the AggR regulon in vivo. ..
  5. Virulence in Enteroaggregative Escherichia coli
    James Nataro; Fiscal Year: 2007
    ..In this aim, we will take a genomics approach to look for additional AggR-regulated genes, and characterize expression of the AggR regulon in vivo. ..
  6. Live Vector Vaccines Against Agents of Bioterror
    James Nataro; Fiscal Year: 2007
    ..During the term of the award, we will generate a series of vaccine candidates for immediate Phase 1 human trials. ..
  7. Virulence in Enteroaggregative Escherichia coli
    James Nataro; Fiscal Year: 2009
    ..In this aim, we will take a genomics approach to look for additional AggR-regulated genes, and characterize expression of the AggR regulon in vivo. ..
  8. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 2003
    ..abstract_text> ..
  9. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 1999
    ..Successful pursuit of the aims of this grant will result in advances in the understanding of EAggEc as well as mechanisms of persistent diarrhea and malabsorption. ..
  10. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 2000
    ..abstract_text> ..
  11. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 2001
    ..abstract_text> ..
  12. VIRULENCE IN ENTEROAGGREGATIVE E COLI
    James Nataro; Fiscal Year: 2002
    ..abstract_text> ..
  13. Virulence in Enteroaggregative Escherichia coli
    James P Nataro; Fiscal Year: 2010
    ..In this project, we will further characterize the mechanisms of EAEC- induced diarrhea with an eye toward development of effective preventive measures. ..