Research Topics
Genomes and Genes | Eiko Nakamaru-OgisoSummaryAffiliation: University of Pennsylvania Country: USA Publications
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Publications
Characterization of the iron-sulfur cluster coordinated by a cysteine cluster motif (CXXCXXXCX27C) in the Nqo3 subunit in the proton-translocating NADH-quinone oxidoreductase (NDH-1) of Thermus thermophilus HB-8Eiko Nakamaru-Ogiso
Department of Molecular and Experimental Medicine, Division of Biochemistry, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 277:1680-8. 2002..Considering the fact that the same motif coordinates only tetranuclear clusters in other enzymes so far known, we propose that the CXXCXXXCX(27)C motif in the Nqo3 subunit most likely ligates the [4Fe-4S] cluster...
Iron-sulfur cluster N5 is coordinated by an HXXXCXXCXXXXXC motif in the NuoG subunit of Escherichia coli NADH:quinone oxidoreductase (complex I)Eiko Nakamaru-Ogiso
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 283:25979-87. 2008..These data confirmed that, contrary to the misassignment claim, cluster N5 has a unique coordination with His(Cys)(3) ligands in NuoG...
Novel biochemical manipulation of brain serotonin reveals a role of serotonin in the circadian rhythm of sleep-wake cyclesEiko Nakamaru-Ogiso
Department of Biomedical Chemistry, Institute of International Health, The University of Tokyo Graduate School of Medicine, Tokyo, Japan
Eur J Neurosci 35:1762-70. 2012..Our results suggest that 5-HT with a rapid turnover rate plays an important role in the circadian rhythm of sleep-wake cycles...- The membrane subunit NuoL(ND5) is involved in the indirect proton pumping mechanism of Escherichia coli complex IEiko Nakamaru-Ogiso
Johnson Research Foundation, Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 285:39070-8. 2010..The electron transfer activities were basically unaffected by EIPA in both control and mutants. Taken together, our data strongly indicate that the NuoL subunit is involved in the indirect coupling mechanism... - The ND2 subunit is labeled by a photoaffinity analogue of asimicin, a potent complex I inhibitorEiko Nakamaru-Ogiso
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
FEBS Lett 584:883-8. 2010..The cross-linking was blocked by rotenone. This is the first finding that ND2 was photo-crosslinked with a potent complex I inhibitor, suggesting its involvement in the inhibitor/quinone-binding... - Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coliMou Chieh Kao
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Biochemistry 44:9545-54. 2005..Possible roles of these arginine residues and other conserved residues in the NuoK subunit for NDH-1 function were discussed... - Characterization of the membrane domain subunit NuoJ (ND6) of the NADH-quinone oxidoreductase from Escherichia coli by chromosomal DNA manipulationMou Chieh Kao
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Biochemistry 44:3562-71. 2005..Together with the results on mutations related to human diseases, possible functional roles of the NuoJ subunit have been discussed... - Functional roles of four conserved charged residues in the membrane domain subunit NuoA of the proton-translocating NADH-quinone oxidoreductase from Escherichia coliMou Chieh Kao
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 279:32360-6. 2004..The possible functional roles of residues Asp-79 and Glu-81 were discussed... - Characterization of the NuoM (ND4) subunit in Escherichia coli NDH-1: conserved charged residues essential for energy-coupled activitiesJesus Torres-Bacete
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 282:36914-22. 2007..The data suggest that these His are not involved in the catalytic Q-binding. Functional roles of NuoM and advantages of NDH-1 research as a model for mitochondrial complex I study have been discussed... - Possibility of transkingdom gene therapy for complex I diseasesTakao Yagi
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
Biochim Biophys Acta 1757:708-14. 2006..The use of NDI1 as a potential molecular therapy for complex I-deficient diseases is briefly discussed, including the proposed animal model... - Neuroprotective effect of long-term NDI1 gene expression in a chronic mouse model of Parkinson disorderJennifer Barber-Singh
The Scripps Research Institute, Department of Molecular and Experimental Medicine, La Jolla, California 92037, USA
Rejuvenation Res 12:259-67. 2009..The data presented in this study demonstrate a protective effect of the NDI1 gene in dopaminergic neurons, suggesting its therapeutic potential for Parkinson-like disorders... - Electron transfer in subunit NuoI (TYKY) of Escherichia coli NADH:quinone oxidoreductase (NDH-1)Prem Kumar Sinha
Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 287:17363-73. 2012..Replacement of Ile-100 and Ile-94 with Ala/Gly did not affect the electron transfer activity significantly. It is concluded that conserved Ile-100 and Ile-94 are not essential for the electron transfer... - Can a single subunit yeast NADH dehydrogenase (Ndi1) remedy diseases caused by respiratory complex I defects?Takao Yagi
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Reseach Institute, La Jolla, California 92037, USA
Rejuvenation Res 9:191-7. 2006..The Ndi1 protein has a great potential as a molecular remedy for complex I deficiencies... - Characterization of the iron-sulfur cluster N7 (N1c) in the subunit NuoG of the proton-translocating NADH-quinone oxidoreductase from Escherichia coliEiko Nakamaru-Ogiso
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 280:301-7. 2005.... - Amino acid residues associated with cluster N3 in the NuoF subunit of the proton-translocating NADH-quinone oxidoreductase from Escherichia coliIsabel Velazquez
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
FEBS Lett 579:3164-8. 2005..92, 1.95, and 2.01) in NuoE subunit. These clusters were assigned to clusters N3 and N1a, respectively. Based on the site-directed mutagenesis experiments, we identified that cluster N3 is ligated to the 351Cx2Cx2Cx40C398 motif... - Amiloride inhibition of the proton-translocating NADH-quinone oxidoreductase of mammals and bacteriaEiko Nakamaru-Ogiso
Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, 10550 Torrey Pines Road, La Jolla, CA 92037, USA
FEBS Lett 549:43-6. 2003.... - A new hypothesis on the simultaneous direct and indirect proton pump mechanisms in NADH-quinone oxidoreductase (complex I)Tomoko Ohnishi
Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6059, USA
FEBS Lett 584:4131-7. 2010..We propose a revised hypothesis that each of the "direct" and the "indirect" proton pumps transports 2H(+) per 2e(-)... - Roles of bound quinone in the single subunit NADH-quinone oxidoreductase (Ndi1) from Saccharomyces cerevisiaeTetsuo Yamashita
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 282:6012-20. 2007..The data suggest that Ndi1 bears at least two distinct Q sites: one for bound Q and the other for catalytic Q... - Characterization of cluster N5 as a fast-relaxing [4Fe-4S] cluster in the Nqo3 subunit of the proton-translocating NADH-ubiquinone oxidoreductase from Paracoccus denitrificansTakahiro Yano
Johnson Research Foundation, Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
J Biol Chem 278:15514-22. 2003..Hence, we propose that the His-coordinated [4Fe-4S] cluster corresponds to cluster N5... - In vivo complementation of complex I by the yeast Ndi1 enzyme. Possible application for treatment of Parkinson diseaseByoung Boo Seo
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 281:14250-5. 2006..These results indicate that the expressed Ndi1 protein elicits resistance to MPTP-induced neuronal injury. The present study is the first successful demonstration of complementation of complex I by the Ndi1 enzyme in animals... - Functional expression of the single subunit NADH dehydrogenase in mitochondria in vivo: a potential therapy for complex I deficienciesByoung Boo Seo
Division of Biochemistry, Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA
Hum Gene Ther 15:887-95. 2004..The data indicate that the NDI1 gene will be a promising therapeutic tool in the treatment of encephalomyopathies and neurodegenerative diseases caused by complex I impairments... - Were there any "misassignments" among iron-sulfur clusters N4, N5 and N6b in NADH-quinone oxidoreductase (complex I)?Tomoko Ohnishi
Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6059, USA
Biochim Biophys Acta 1777:703-10. 2008..As to the claim of "misassignment" between clusters N4 and N6b, that was not a possibility because our mutagenesis systems did not contain cluster N6b. Therefore, we believe that we have not made any "misassignment" in our work... - Critical roles of subunit NuoH (ND1) in the assembly of peripheral subunits with the membrane domain of Escherichia coli NDH-1Prem Kumar Sinha
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 284:9814-23. 2009..Implications for the involvement of NuoH (ND1) in maintaining the structure and function of NDH-1 are discussed... - Protection by the NDI1 gene against neurodegeneration in a rotenone rat model of Parkinson's diseaseMathieu Marella
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America
PLoS ONE 3:e1433. 2008..The present study shows, for the first time, the powerful neuroprotective effect offered by the Ndi1 enzyme in a rotenone rat model of PD... - Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesisYan Zhang
Department of Medicine, Division of Hematology Oncology, University of Pennsylvania, Philadelphia, PA 19104, USA
Mol Cell Biol 28:5569-82. 2008.... - Serotonergic integration of circadian clock and ultradian sleep-wake cyclesHiroyuki Miyamoto
Department of Biomedical Chemistry, Institute of International Health, The University of Tokyo Graduate School of Medicine, Bunkyo ku, Tokyo 113 0033, Japan
J Neurosci 32:14794-803. 2012..These results suggest that the serotonergic system enables the BF/POA to couple the SCN circadian signal to ultradian sleep-wake cycles, thereby providing a potential link between circadian rhythms and psychiatric disorders... - Rhodoquinone reaction site of mitochondrial complex I, in parasitic helminth, Ascaris suumTetsuo Yamashita
Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Biochim Biophys Acta 1608:97-103. 2004..4 and 7.2. Our results indicate that although A. suum complex I uses both RQ(2) and UQ(2) as an electron acceptor, the manner of reaction (or binding) of the two quinones differs... - The ND5 subunit was labeled by a photoaffinity analogue of fenpyroximate in bovine mitochondrial complex IEiko Nakamaru-Ogiso
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
Biochemistry 42:746-54. 2003..These results suggest that the ND5 subunit is involved in construction of the inhibitor- and quinone-binding site(s). Furthermore, it seems likely that the ND5 subunit may participate in H(+)(Na(+)) translocation in coupling site 1... - A single-subunit NADH-quinone oxidoreductase renders resistance to mammalian nerve cells against complex I inhibitionByoung Boo Seo
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
Mol Ther 6:336-41. 2002..It is conceivable that the NDI1 gene will be a promising tool in the treatment of neurodegenerative conditions caused by complex I inhibition... - Pentavalent uranium trans-dihalides and -pseudohalidesAndrew J Lewis
P Roy and Diana T Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, 19104, USA
Chem Commun (Camb) 48:4977-9. 2012..Uranium(v) mixed-halides are also synthesized by successive one-electron oxidation reactions... - Alteration of the bis-tetrahydrofuran core stereochemistries in asimicin can affect the cytotoxicitySubhash C Sinha
The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
J Med Chem 51:7045-8. 2008.... - Total synthesis of piericidin A1 and B1 and key analoguesMartin J Schnermann
Departments of Chemistry and Molecular and Experimental Medicine, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Am Chem Soc 128:11799-807. 2006.... - Probucol ameliorates renal and metabolic sequelae of primary CoQ deficiency in Pdss2 mutant miceMarni J Falk
Division of Human Genetics, Department of Pediatrics, The Children s Hospital of Philadelphia, Philadelphia, PA, USA
EMBO Mol Med 3:410-27. 2011.... - A comparison of the effects of symmetry and magnetoanisotropy on paramagnetic relaxation in related dysprosium single ion magnetsUrsula J Williams
P Roy and Diana T Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Chem Commun (Camb) 48:5593-5. 2012..Comparison of these compounds with the seminal phthalocyanine complex [Dy(Pc)(2)](-) shows the azaannulide complexes are more susceptible to relaxation through non-thermal pathways... - NMNAT1 mutations cause Leber congenital amaurosisMarni J Falk
Department of Pediatrics, Division of Human Genetics, The Children s Hospital of Philadelphia, Pennsylvania, USA
Nat Genet 44:1040-5. 2012..These results are the first to link an NMNAT isoform to disease in humans and indicate that NMNAT1 mutations cause LCA... - Obligatory role for complex I inhibition in the dopaminergic neurotoxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)Jason R Richardson
Department of Environmental and Occupational Medicine, University of Medicine and Dentistry, New Jersey Robert Wood Johnson Medical School and Environmental and Occupational Health Sciences Institute, Piscataway, New Jersey 08854, USA
Toxicol Sci 95:196-204. 2007..Furthermore, combined with reports of a complex I defect in Parkinson's disease (PD) patients, the present study affirms the utility of MPTP in understanding the molecular mechanisms underlying dopaminergic neurodegeneration in PD...