Peter D Nagy


Affiliation: University of Kentucky
Country: USA


  1. Inaba J, Nagy P. Tombusvirus RNA replication depends on the TOR pathway in yeast and plants. Virology. 2018;519:207-222 pubmed publisher
    ..Altogether, the TOR pathway is crucial for TBSV to replicate efficiently in hosts. ..
  2. Nagy P. Viral sensing of the subcellular environment regulates the assembly of new viral replicase complexes during the course of infection. J Virol. 2015;89:5196-9 pubmed publisher
    ..These regulatory mechanisms might explain how tombusviruses could adjust the efficiency of RNA replication and new VRC assembly to the limiting resources of the host cells during infections. ..
  3. Pogany J, Nagy P. Activation of Tomato Bushy Stunt Virus RNA-Dependent RNA Polymerase by Cellular Heat Shock Protein 70 Is Enhanced by Phospholipids In Vitro. J Virol. 2015;89:5714-23 pubmed publisher
    ..Thus, the activation of the viral RdRp likely depends on multiple host components in infected cells. ..
  4. Barajas D, Kovalev N, Qin J, Nagy P. Novel mechanism of regulation of tomato bushy stunt virus replication by cellular WW-domain proteins. J Virol. 2015;89:2064-79 pubmed publisher
    ..Altogether, Rsp5 is a CIRF against a tombusvirus, and it possibly has a regulatory function during viral replication in infected cells. ..
  5. Xu K, Nagy P. Expanding use of multi-origin subcellular membranes by positive-strand RNA viruses during replication. Curr Opin Virol. 2014;9:119-26 pubmed publisher
    ..Overall, these viruses show remarkable flexibility in utilizing various subcellular membranes for replication under different conditions. ..
  6. Barajas D, Xu K, Sharma M, Wu C, Nagy P. Tombusviruses upregulate phospholipid biosynthesis via interaction between p33 replication protein and yeast lipid sensor proteins during virus replication in yeast. Virology. 2014;471-473:72-80 pubmed publisher
    ..Overall, this work reveals that a (+)RNA virus reprograms the phospholipid biosynthesis pathway in a unique way to facilitate its replication in yeast cells. ..
  7. Xu K, Nagy P. RNA virus replication depends on enrichment of phosphatidylethanolamine at replication sites in subcellular membranes. Proc Natl Acad Sci U S A. 2015;112:E1782-91 pubmed publisher
    ..In summary, various (+)RNA viruses might subvert PE to build membrane-bound VRCs for robust replication in PE-enriched membrane microdomains. ..
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    Nagy P. Tombusvirus-Host Interactions: Co-Opted Evolutionarily Conserved Host Factors Take Center Court. Annu Rev Virol. 2016;3:491-515 pubmed
    ..Altogether, these advances in our understanding of tombusvirus-host interactions are broadly applicable to many other viruses, which also usurp conserved host factors for various viral processes. ..
  9. Xu K, Nagy P. Sterol Binding by the Tombusviral Replication Proteins Is Essential for Replication in Yeast and Plants. J Virol. 2017;91: pubmed publisher
    ..Together, the results further strengthen the model that cellular sterols are essential as proviral lipids during viral replication. ..

More Information


  1. Nagy P. Yeast as a model host to explore plant virus-host interactions. Annu Rev Phytopathol. 2008;46:217-42 pubmed publisher
  2. Prasanth K, Barajas D, Nagy P. The proteasomal Rpn11 metalloprotease suppresses tombusvirus RNA recombination and promotes viral replication via facilitating assembly of the viral replicase complex. J Virol. 2015;89:2750-63 pubmed publisher
    ..Taken together, the results show that the co-opted cellular Rpn11 is a critical host factor for tombusviruses by regulating viral replication and genetic recombination. ..
  3. Kovalev N, de Castro Martín I, Pogany J, Barajas D, Pathak K, Risco C, et al. Role of Viral RNA and Co-opted Cellular ESCRT-I and ESCRT-III Factors in Formation of Tombusvirus Spherules Harboring the Tombusvirus Replicase. J Virol. 2016;90:3611-26 pubmed publisher
    ..The work could be of broad interest in virology and beyond. ..
  4. Imura Y, Molho M, Chuang C, Nagy P. Cellular Ubc2/Rad6 E2 ubiquitin-conjugating enzyme facilitates tombusvirus replication in yeast and plants. Virology. 2015;484:265-75 pubmed publisher
    ..We provide evidence that Rad6p is involved in promoting the subversion of Vps23p and Vps4p ESCRT proteins for viral replicase complex assembly. ..
  5. Prasanth K, Kovalev N, de Castro Martín I, Baker J, Nagy P. Screening a yeast library of temperature-sensitive mutants reveals a role for actin in tombusvirus RNA recombination. Virology. 2016;489:233-42 pubmed publisher
    ..Overall, this work shows that the actin network affects tombusvirus recombination in yeast and plant cells. ..
  6. Nagy P, Pogany J, Xu K. Cell-Free and Cell-Based Approaches to Explore the Roles of Host Membranes and Lipids in the Formation of Viral Replication Compartment Induced by Tombusviruses. Viruses. 2016;8:68 pubmed publisher
    ..Remarkably, all the known steps in TBSV replication are dependent on cellular lipids and co-opted membranes. ..
  7. Nagy P, Wang R, Pogany J, Hafren A, Makinen K. Emerging picture of host chaperone and cyclophilin roles in RNA virus replication. Virology. 2011;411:374-82 pubmed publisher
    ..This review also discusses the emerging roles of cyclophilins, which are peptidyl-prolyl isomerases with chaperone functions, in replication of selected (+)RNA viruses. ..
  8. Nagy P, Pogany J. Global genomics and proteomics approaches to identify host factors as targets to induce resistance against Tomato bushy stunt virus. Adv Virus Res. 2010;76:123-77 pubmed publisher
  9. Shapka N, Nagy P. The AU-rich RNA recombination hot spot sequence of Brome mosaic virus is functional in tombusviruses: implications for the mechanism of RNA recombination. J Virol. 2004;78:2288-300 pubmed
    ..In summary, evidence is presented that a tombusvirus can use the recombination signal of BMV. This supports the idea that common AU-rich recombination signals might promote interviral recombination between unrelated viruses...
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    Nagy P, Pogany J. Yeast as a model host to dissect functions of viral and host factors in tombusvirus replication. Virology. 2006;344:211-20 pubmed
    ..The knowledge obtained will likely stimulate development of new antiviral methods as well as other approaches that could make tombusviruses useful tools in biotechnological applications...
  11. Chuang C, Prasanth K, Nagy P. The Glycolytic Pyruvate Kinase Is Recruited Directly into the Viral Replicase Complex to Generate ATP for RNA Synthesis. Cell Host Microbe. 2017;22:639-652.e7 pubmed publisher
    ..Altogether, recruitment of PK and local production of ATP within the replication compartment allow the virus replication machinery an access to plentiful ATP, facilitating robust virus replication. ..
  12. Pogany J, White K, Nagy P. Specific binding of tombusvirus replication protein p33 to an internal replication element in the viral RNA is essential for replication. J Virol. 2005;79:4859-69 pubmed
    ..Since all genera in Tombusviridae encode comparable replicase proteins, these results may be relevant to other members of this large virus family...
  13. Nagy P, Pogany J. The dependence of viral RNA replication on co-opted host factors. Nat Rev Microbiol. 2011;10:137-49 pubmed publisher
    ..This Review presents the recent progress that has been made in understanding the role of co-opted host proteins and membranes during (+)RNA virus replication, and discusses common themes employed by different viruses...