Karuppiah Muthumani

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. pmc Immunogenicity of novel consensus-based DNA vaccines against Chikungunya virus
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Vaccine 26:5128-34. 2008
  2. pmc HIV-mediated phosphatidylinositol 3-kinase/serine-threonine kinase activation in APCs leads to programmed death-1 ligand upregulation and suppression of HIV-specific CD8 T cells
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Immunol 187:2932-43. 2011
  3. pmc Human immunodeficiency virus type 1 Nef induces programmed death 1 expression through a p38 mitogen-activated protein kinase-dependent mechanism
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Virol 82:11536-44. 2008
  4. pmc The HIV-1 Vpr and glucocorticoid receptor complex is a gain-of-function interaction that prevents the nuclear localization of PARP-1
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Nat Cell Biol 8:170-9. 2006
  5. ncbi request reprint Novel engineered HIV-1 East African Clade-A gp160 plasmid construct induces strong humoral and cell-mediated immune responses in vivo
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Virology 314:134-46. 2003
  6. ncbi request reprint Adenovirus encoding HIV-1 Vpr activates caspase 9 and induces apoptotic cell death in both p53 positive and negative human tumor cell lines
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, PA 19104, USA
    Oncogene 21:4613-25. 2002
  7. ncbi request reprint HIV-1 Viral protein-r (Vpr) protects against lethal superantigen challenge while maintaining homeostatic T cell levels in vivo
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 505 Stellar Chance Building, 422 Curie Boulevard, Philadelphia, PA 19104, USA
    Mol Ther 12:910-21. 2005
  8. ncbi request reprint Immunogenicity testing of a novel engineered HIV-1 envelope gp140 DNA vaccine construct
    Sanjeev Kumar
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6100, USA
    DNA Cell Biol 25:383-92. 2006
  9. pmc Co-immunization with an optimized plasmid-encoded immune stimulatory interleukin, high-mobility group box 1 protein, results in enhanced interferon-gamma secretion by antigen-specific CD8 T cells
    Gowtham Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Immunology 128:e612-20. 2009
  10. pmc HIV-1 Nef-induced FasL induction and bystander killing requires p38 MAPK activation
    Karuppiah Muthumani
    University of Pennsylvania School of Medicine, Department of Pathology and Laboratory Medicine 422 Curie Blvd, Philadelphia, PA 19104, USA
    Blood 106:2059-68. 2005

Collaborators

Detail Information

Publications33

  1. pmc Immunogenicity of novel consensus-based DNA vaccines against Chikungunya virus
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Vaccine 26:5128-34. 2008
    ..Taken together, these data support further study of the use of consensus CHIK antigens in a potential vaccine cocktail...
  2. pmc HIV-mediated phosphatidylinositol 3-kinase/serine-threonine kinase activation in APCs leads to programmed death-1 ligand upregulation and suppression of HIV-specific CD8 T cells
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Immunol 187:2932-43. 2011
    ..These data identify a novel mechanism by which HIV exploits the immunosuppressive PD-1 pathway and suggest a new role for virus-infected cells in the local corruption of immune responses required for viral suppression...
  3. pmc Human immunodeficiency virus type 1 Nef induces programmed death 1 expression through a p38 mitogen-activated protein kinase-dependent mechanism
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Virol 82:11536-44. 2008
    ..These data demonstrate an important signaling event of Nef in HIV-1 pathogenesis...
  4. pmc The HIV-1 Vpr and glucocorticoid receptor complex is a gain-of-function interaction that prevents the nuclear localization of PARP-1
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Nat Cell Biol 8:170-9. 2006
    ..These data provide important insights into Vpr biology and its role in HIV pathogenesis...
  5. ncbi request reprint Novel engineered HIV-1 East African Clade-A gp160 plasmid construct induces strong humoral and cell-mediated immune responses in vivo
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Virology 314:134-46. 2003
    ..This study suggests a simple strategy for improving the expression and immunogenicity of HIV subtype-specific envelope antigens as plasmid or vector-borne immunogens...
  6. ncbi request reprint Adenovirus encoding HIV-1 Vpr activates caspase 9 and induces apoptotic cell death in both p53 positive and negative human tumor cell lines
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, PA 19104, USA
    Oncogene 21:4613-25. 2002
    ..Such a vector may give insight into approaches designed to limit the growth of pathogenic human cells...
  7. ncbi request reprint HIV-1 Viral protein-r (Vpr) protects against lethal superantigen challenge while maintaining homeostatic T cell levels in vivo
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 505 Stellar Chance Building, 422 Curie Boulevard, Philadelphia, PA 19104, USA
    Mol Ther 12:910-21. 2005
    ..Understanding the biology of Vpr's activities in this model may allow for new insight into potential mechanisms of hyperinflammatory disease and into Vpr pathobiology in the context of HIV infection...
  8. ncbi request reprint Immunogenicity testing of a novel engineered HIV-1 envelope gp140 DNA vaccine construct
    Sanjeev Kumar
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6100, USA
    DNA Cell Biol 25:383-92. 2006
    ..4 and 1.5% proliferation was observed in CD4+, CD8+, and CCR+ memory T cells, respectively. Therefore, this engineered synthetic optimized env DNA vaccine may be useful in DNA vaccine and other studies of HIV-1 immunogenicity...
  9. pmc Co-immunization with an optimized plasmid-encoded immune stimulatory interleukin, high-mobility group box 1 protein, results in enhanced interferon-gamma secretion by antigen-specific CD8 T cells
    Gowtham Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Immunology 128:e612-20. 2009
    ..Our results show that co-immunization with HMGB1 can have a strong adjuvant activity, driving strong cellular and humoral immunity that may be an effective immunological adjuvant in DNA vaccination against HIV-1...
  10. pmc HIV-1 Nef-induced FasL induction and bystander killing requires p38 MAPK activation
    Karuppiah Muthumani
    University of Pennsylvania School of Medicine, Department of Pathology and Laboratory Medicine 422 Curie Blvd, Philadelphia, PA 19104, USA
    Blood 106:2059-68. 2005
    ..Therefore, a linear pathway for Nef-induced FasL expression that encompasses p38 and AP-1 has been elucidated. Furthermore, chemical inhibition of the p38 pathway attenuates HIV-1-mediated bystander killing of CD8 cells in vitro...
  11. ncbi request reprint HIV-1 Vpr: regulator of viral survival
    Khanh P Thieu
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Curr HIV Res 7:153-62. 2009
    ..Vpr may therefore be considered among the most important accessory proteins encoded by HIV...
  12. ncbi request reprint HIV-1 Vpr and anti-inflammatory activity
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    DNA Cell Biol 23:239-47. 2004
    ..These activities are worthy of further examination for control of hyperinflammatory and hyperproliferative conditions...
  13. ncbi request reprint Anti-tumor activity mediated by protein and peptide transduction of HIV viral protein R (Vpr)
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    Cancer Biol Ther 8:180-7. 2009
    ....
  14. pmc A highly optimized DNA vaccine confers complete protective immunity against high-dose lethal lymphocytic choriomeningitis virus challenge
    Devon J Shedlock
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Vaccine 29:6755-62. 2011
    ..This is the first non-infectious vaccine conferring complete protective immunity up to 8 weeks after vaccination and demonstrates the potential of 'next-generation' DNA vaccines...
  15. pmc Ki-67 staining for determination of rhesus macaque T cell proliferative responses ex vivo
    Devon J Shedlock
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Cytometry A 77:275-84. 2010
    ..We show that this method is a valid tool for measuring RM antigen-specific cellular proliferation ex vivo and can be used as an alternative to CFSE dilution assays...
  16. ncbi request reprint HIV-1 Vpr inhibits the maturation and activation of macrophages and dendritic cells in vitro
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, PA 19104, USA
    Int Immunol 17:103-16. 2005
    ..These studies have importance for understanding the interaction of HIV with the host immune system...
  17. ncbi request reprint Mechanism of HIV-1 viral protein R-induced apoptosis
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, 505 Stellar Chance Laboratories, 422 Curie Boulevard, Philadelphia, PA 19104, USA
    Biochem Biophys Res Commun 304:583-92. 2003
    ..Vpr may interact with the adenine nucleotide translocator (ANT) to prompt this cascade. The role of Vpr-induced apoptosis in HIV pathogenesis is considered...
  18. ncbi request reprint Suppression of HIV-1 viral replication and cellular pathogenesis by a novel p38/JNK kinase inhibitor
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    AIDS 18:739-48. 2004
    ..To analyze a novel compound, which inhibits serine-threonine protein kinase p38, for its possible bioactivity against HIV-1 infection...
  19. pmc A DNA vaccine against chikungunya virus is protective in mice and induces neutralizing antibodies in mice and nonhuman primates
    Karthik Mallilankaraman
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS Negl Trop Dis 5:e928. 2011
    ..These data suggest a protective role for nAb against CHIKV disease and support further study of envelope-based CHIKV DNA vaccines...
  20. ncbi request reprint Next generation DNA vaccines for HIV-1
    J D Boyer
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Liposome Res 12:137-42. 2002
    ..Our studies show that both costimulation as well as cytokine signals can be harnessed for more potent vaccine development. These results have important implications for the design of vaccines for prophylaxis and therapy...
  21. ncbi request reprint SIV DNA vaccine co-administered with IL-12 expression plasmid enhances CD8 SIV cellular immune responses in cynomolgus macaques
    Jean D Boyer
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    J Med Primatol 34:262-70. 2005
    ..The vaccine immune responses contained both a CD8 component as well a CD4 component. The adjuvanted DNA vaccines illustrate that IL-12 enhances a CD8 vaccine immune response, however, different cellular profiles...
  22. ncbi request reprint DR5 activation of caspase-8 induces DC maturation and immune enhancement in vivo
    Michael A Chattergoon
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Mol Ther 16:419-26. 2008
    ....
  23. pmc DNA vaccines for targeting bacterial infections
    Mariana Ingolotti
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Expert Rev Vaccines 9:747-63. 2010
    ..In addition, it provides an updated summary of the methods used to enhance immunogenicity from codon optimization and adjuvants to delivery techniques including electroporation and use of nanoparticles...
  24. doi request reprint The HIV-1 Vif protein mediates degradation of Vpr and reduces Vpr-induced cell cycle arrest
    JiangFang Wang
    Division of Rheumatology, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
    DNA Cell Biol 27:267-77. 2008
    ..Together, these data show that Vif mediates the degradation of Vpr and modulates Vpr-induced G2 arrest in HIV-1-infected T-cells...
  25. ncbi request reprint The HIV-1 accessory gene vpr can inhibit antigen-specific immune function
    Karuppiah Muthumani
    University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    DNA Cell Biol 21:689-95. 2002
    ..Further study of the biology of Vpr will likely have importance for a clearer understanding of host pathogenesis as well as have important implications for HIV vaccine development...
  26. pmc Chikungunya: a potentially emerging epidemic?
    Michelle M Thiboutot
    Department of Earth and Environmental Sciences, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS Negl Trop Dis 4:e623. 2010
    ..In this review, we summarize the evidence supporting these concepts...
  27. doi request reprint Nonstructural protein 2 (nsP2) of Chikungunya virus (CHIKV) enhances protective immunity mediated by a CHIKV envelope protein expressing DNA Vaccine
    Huihui Bao
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Viral Immunol 26:75-83. 2013
    ..In sum, these novel data imply that the addition of nsP2 to the pEnv vaccine enhances anti-CHIKV-Env immune responses and maybe useful to include in future CHIKV clinical vaccination strategies...
  28. doi request reprint HIV-1 viral genes and mitochondrial apoptosis
    Devon J Shedlock
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Apoptosis 13:1088-99. 2008
    ..Here we review the mechanisms by which HIV affects the mitochondrion, and suggest that various viral-associated genes may directly regulate apoptotic cell death...
  29. ncbi request reprint HIV-1 Vpr: enhancing sensitivity of tumors to apoptosis
    Karuppiah Muthumani
    Dept of Pathology and Lab Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Curr Drug Deliv 1:335-44. 2004
    ..Among these are its ability to induce cell cycle arrest, inhibit inflammation, provoke p53 independent apoptosis, and selective killing of rapidly dividing cells...
  30. pmc Selected approaches for increasing HIV DNA vaccine immunogenicity in vivo
    Natalie A Hutnick
    Department of Pathology and Laboratory Medicine, Perlman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States
    Curr Opin Virol 1:233-40. 2011
    ....
  31. ncbi request reprint A Gag-Pol/Env-Rev SIV239 DNA vaccine improves CD4 counts, and reduce viral loads after pathogenic intrarectal SIV(mac)251 challenge in rhesus Macaques
    Karuppiah Muthumani
    Department of Pathology and Laboratory of Medicine, University of Pennsylvania, 505 Stellar Chance Laboratories, 422 Curie Blvd, Philadelphia, PA 19104, USA
    Vaccine 21:629-37. 2003
    ..Furthermore, vaccinated animals exhibited protection against CD4(+) T-cell loss...
  32. ncbi request reprint HIV-1 Vpr induces apoptosis through caspase 9 in T cells and peripheral blood mononuclear cells
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 277:37820-31. 2002
    ..Bcl-2 overexpressing cells succumb to Vpr-induced apoptosis. These studies illustrate that Vpr induces a mitochondria-dependent apoptotic pathway that is distinct from apoptosis driven by the Fas-FasL pathway...
  33. ncbi request reprint Issues for improving multiplasmid DNA vaccines for HIV-1
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Vaccine 20:1999-2003. 2002
    ..It is likely that the combination of such advancements will significantly improve the clinical phenotype of this important vaccine modality...