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Genomes and Genes | Robert C MurphySummaryAffiliation: University of Colorado Health Sciences Center Country: USA Publications
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Publications
Electrospray mass spectrometry of phospholipidsMelissa Pulfer
Department of Pediatrics, Division of Cell Biology, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado 80206, USA
Mass Spectrom Rev 22:332-64. 2003..Several applications of mass spectrometry applied to phospholipid analysis are presented as they apply to physiology as well as pathophysiology...
Detection of the abundance of diacylglycerol and triacylglycerol molecular species in cells using neutral loss mass spectrometryRobert C Murphy
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA
Anal Biochem 366:59-70. 2007..A 50:1-TAG containing 18:1 was found to increase significantly over a 24-h time course after Kdo(2)-lipid A exposure, whereas an isobaric 50:1-TAG containing 16:1 did not change relative to controls...- Cholesterol, reactive oxygen species, and the formation of biologically active mediatorsRobert C Murphy
Department of Pharmacology, University of Colorado Denver Health Sciences Center, Aurora, Colorado 80045-6511, USA
J Biol Chem 283:15521-5. 2008 - Electrospray ionization and tandem mass spectrometry of eicosanoidsRobert C Murphy
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Mail Stop 8303, P.O. Box 6511, Aurora, CO 80045-0511, USA
Anal Biochem 346:1-42. 2005 - The 5-lipoxygenase pathway is required for acute lung injury following hemorrhagic shockJohn C Eun
Department of Surgery, University of Colorado Denver, Aurora, Colorado 80204, USA
Shock 37:599-604. 2012..This pathway could represent a new target for pharmacological intervention to reduce lung damage following severe primary injury... 
Characterization of oxidized phosphatidylethanolamine derived from RAW 264.7 cells using 4-(dimethylamino)benzoic acid derivativesKarin A Zemski Berry
Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045, USA
Eur J Mass Spectrom (Chichester, Eng) 16:463-70. 2010..The stable isotope labeled derivatives permitted assessment of relative quantitative changes in oxidized lipids based upon ion abundance...- MALDI imaging MS of phospholipids in the mouse lungKarin A Zemski Berry
Department of Pharmacology, University of Colorado Denver, 12801 East 17 Avenue, Mail Stop 8303, Aurora, CO 80045, USA
J Lipid Res 52:1551-60. 2011..This technique revealed unexpected high concentrations of arachidonate- and docosahexaenoate-containing phospholipids lining the airways in pulmonary tissue, which could serve as precursors of lipid mediators affecting airways biology... - Qualitative analysis and quantitative assessment of changes in neutral glycerol lipid molecular species within cellsJessica Krank
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado
Methods Enzymol 432:1-20. 2007..An example of the application of this method was in the quantitative analysis of TAG and DAG molecular species present in RAW 264.7 cells treated with a Toll-4 receptor ligand, Kdo2-lipid A, in a time course study... - Lipid mediators in cerebral spinal fluid of traumatic brain injured patientsSantiago E Farias
Department of Pharmacology, University of Colorado at Denver, Anschutz Medical Campus, Aurora, CO, USA
J Trauma 71:1211-8. 2011..Thus, levels of these metabolites in normal and pathologic conditions in the brain are particularly relevant in understanding the transition to disease... - Transcellular biosynthesis of cysteinyl leukotrienes in vivo during mouse peritoneal inflammationSimona Zarini
Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045, USA
Proc Natl Acad Sci U S A 106:8296-301. 2009..These results highlight the potential relevance of transcellular exchange of LTA(4) for the synthesis of LTs mediating biological activities during inflammatory events in vivo... - Lysophospholipid acyltransferases and arachidonate recycling in human neutrophilsMiguel A Gijón
Department of Pharmacology, University of Colorado Denver, Aurora, Colorado 80045, USA
J Biol Chem 283:30235-45. 2008..These results strongly implicate MBOAT5 and MBOAT7 in arachidonate recycling, thus regulating free arachidonic acid levels and leukotriene synthesis in neutrophils... - Biosynthesis of eicosanoids and transcellular metabolism of leukotrienes in murine bone marrow cellsMiguel A Gijón
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045 6511, USA
J Lipid Res 48:716-25. 2007..These data demonstrate that murine bone marrow PMNs are a valid model for the study of LT biosynthesis, which now offers the possibility to investigate specific biochemical pathways through the use of transgenic mice... - Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspIJ Paul Kirwan
Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P.O. Box 8511 MS8303, Aurora, CO 80045, USA
J Bacteriol 188:784-8. 2006..Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone... - Effects of acrolein on leukotriene biosynthesis in human neutrophilsKarin A Zemski Berry
Department of Pharmacology, MSC 8303, University of Colorado Denver, RC1 South, L18 6120, 12801 East 17th Avenue, P O Box 6511, Aurora, Colorado 80045, USA
Chem Res Toxicol 21:2424-32. 2008.... - MALDI mass spectrometric imaging of lipids in rat brain injury modelsJoseph A Hankin
Department of Pharmacology, University of Colorado Denver, Aurora, CO, USA
J Am Soc Mass Spectrom 22:1014-21. 2011..These studies reveal the value of MALDI IMS to examine tissues for changes in lipid biochemistry and will provide data needed to eventually understand the biochemical mechanisms relevant to tissue injury... - Injury-related production of cysteinyl leukotrienes contributes to brain damage following experimental traumatic brain injurySantiago Farias
Department of Pharmacology, University of Colorado at Denver, Anschutz Medical Campus, Aurora, Colorado 80045, USA
J Neurotrauma 26:1977-86. 2009.... - Transcellular biosynthesis of cysteinyl leukotrienes in rat neuronal and glial cellsSantiago E Farias
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado, and the Denver VA Medical Center, Denver, Colorado, USA
J Neurochem 103:1310-8. 2007..The formation of LTC(4) through this mechanism may be relevant in the genesis and progression of the inflammatory response as a result of brain injury... - Eicosanoid transcellular biosynthesis: from cell-cell interactions to in vivo tissue responsesGiancarlo Folco
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Mail Stop 8303, 12801 E. 17th Avenue, P.O. Box 6511, Aurora, CO 80045-0511, USA
Pharmacol Rev 58:375-88. 2006..Experiments for transcellular events taking place in vivo that reveal the true complexity of eicosanoid biosynthesis within tissues are also reviewed... - Biosynthesis and metabolism of leukotrienesRobert C Murphy
Department of Pharmacology, Mail Stop 8303, University of Colorado at Denver and Health Sciences Center, 12801 E 17th Avenue, P O Box 6511, Aurora, CO 80045 0511, USA
Biochem J 405:379-95. 2007..These metabolic transformations of the primary leukotrienes are critical for termination of their biological activity, and defects in expression of participating enzymes may be involved in specific genetic disease... - MALDI imaging of lipids after matrix sublimation/depositionRobert C Murphy
Department of Pharmacology, University of Colorado, Denver, CO, USA
Biochim Biophys Acta 1811:970-5. 2011..Specific examples of sublimation/deposition in lipid imaging of various organs including brain, ocular tissue, and kidney are presented... - Separation of cellular nonpolar neutral lipids by normal-phase chromatography and analysis by electrospray ionization mass spectrometryPatrick M Hutchins
Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045 6511, USA
J Lipid Res 49:804-13. 2008..Longer chain CE molecular species did not change in response to the activation of these cells... - Formation of eicosanoids, E2/D2 isoprostanes, and docosanoids following decapitation-induced ischemia, measured in high-energy-microwaved rat brainSantiago E Farias
Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
J Lipid Res 49:1990-2000. 2008..The present study demonstrated increased formation of eicosanoids, E(2)/D(2)-IsoPs, and docosanoids following cerebral ischemia. A balance of these lipid mediators may mediate immediate events of ischemic injury and recovery... - Low-concentration ozone reacts with plasmalogen glycerophosphoethanolamine lipids in lung surfactantKelly M Wynalda
Department of Pharmacology, University of Colorado Denver, MS 8303, 12801 E 17th Ave, Aurora, Colorado 80045, USA
Chem Res Toxicol 23:108-17. 2010..Lysophospholipids, pentadecanal, and nonanal were found to be the primary products of surfactant ozone oxidation... - Imaging of lipid species by MALDI mass spectrometryRobert C Murphy
Department of Pharmacology, MS 8303 University of Colorado Denver, Aurora, CO 80045, USA
J Lipid Res 50:S317-22. 2009..Regional distribution of many different lipid molecular species and Na+ and K+ attachment ions often define anatomical regions within the tissues... - Lipid rafts regulate lipopolysaccharide-induced activation of Cdc42 and inflammatory functions of the human neutrophilMichael B Fessler
Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
J Biol Chem 279:39989-98. 2004..In sum, these findings provide evidence for an important regulatory role of cholesterol in both transmission of the lipopolysaccharide signal and the inflammatory phenotype of the human neutrophil... - Cholesteryl ester acyl oxidation and remodeling in murine macrophages: formation of oxidized phosphatidylcholinePatrick M Hutchins
Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045, USA
J Lipid Res 53:1588-97. 2012..Further experiments revealed these oxPC species were formed chiefly from the hydrolysis of oxidized CE and subsequent reacylation of the oxidized acyl components into PC... - Electrospray MS/MS reveals extensive and nonspecific oxidation of cholesterol esters in human peripheral vascular lesionsPatrick M Hutchins
Department of Pharmacology, University of Colorado at Denver, Aurora, CO 80045, USA
J Lipid Res 52:2070-83. 2011.... - Relationship between MALDI IMS intensity and measured quantity of selected phospholipids in rat brain sectionsJoseph A Hankin
Department of Pharmacology, University of Colorado Denver, Mail Stop 8303, Aurora, Colorado 80045, USA
Anal Chem 82:8476-84. 2010..There was good correlation between the MALDI IMS ion abundance of PC molecular species and the relative abundance of corresponding PC molecular species in microdissected regions analyzed by LC/MS/MS... - Effect of arachidonic acid reacylation on leukotriene biosynthesis in human neutrophils stimulated with granulocyte-macrophage colony-stimulating factor and formyl-methionyl-leucyl-phenylalanineSimona Zarini
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, 12801 E 17th Avenue, Aurora, CO 80045, USA
J Biol Chem 281:10134-42. 2006.... - Quantitative analysis of phospholipids containing arachidonate and docosahexaenoate chains in microdissected regions of mouse brainPaul H Axelsen
Department of Pharmacology, Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
J Lipid Res 51:660-71. 2010..These differences may be related to the differential susceptibility of different brain regions to neurodegenerative disorders... - Stable isotope labeled 4-(dimethylamino)benzoic acid derivatives of glycerophosphoethanolamine lipidsKarin A Zemski Berry
Department of Pharmacology, University of Colorado Denver, Aurora, Colorado 80045, USA
Anal Chem 81:6633-40. 2009..These DMABA NHS ester reagents provide a universal scan for diacyl, ether, and plasmalogen PE lipids that cannot be readily observed otherwise, enable differential labeling, and provide an internal standard for each PE lipid... - Mass spectrometry advances in lipidomica: collision-induced decomposition of Kdo2-lipid ARobert C Murphy
Department of Pharmacology, University of Colorado Health Sciences Center, Campus Box C236, 4200 E 9th Avenue, Denver, CO 80262, USA
Prostaglandins Other Lipid Mediat 77:131-40. 2005..A rather different challenge is that for the analysis of abundant lipid classes, which are composed of multiple molecular species that can approach several hundred under certain circumstances... - Group IVA phospholipase A₂ is necessary for growth cone repulsion and collapseStaci D Sanford
Department of Pediatrics, Neuroscience Program, and Colorado Intellectual and Developmental Disabilities Research Center, University of Colorado School of Medicine, Aurora, Colorado, USA
J Neurochem 120:974-84. 2012..These studies identify a novel, early step in Sema3A-signaling and a PLA(2) necessary for growth cone repulsion and collapse... - Mass spectrometric analysis of long-chain lipidsRobert C Murphy
Department of Pharmacology, University of Colorado Denver, Aurora, USA
Mass Spectrom Rev 30:579-99. 2011.... - Glycerolipid and cholesterol ester analyses in biological samples by mass spectrometryRobert C Murphy
Department of Pharmacology, University of Colorado, Denver, CO, USA
Biochim Biophys Acta 1811:776-83. 2011..Several applications of these approaches are discussed. ... - New applications of mass spectrometry in lipid analysisRobert C Murphy
Department of Pharmacology, University of Colorado Denver, Aurora, Colorado 80045, USA
J Biol Chem 286:25427-33. 2011..Imaging mass spectrometry of lipids in tissues has opened new insights into the distribution of lipid molecular species with promising application to study pathophysiological events and diseases... - Working towards an exegesis for lipids in biologyH Alex Brown
Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
Nat Chem Biol 5:602-6. 2009.... - Determination of leukotriene A(4) stabilization by S100A8/A9 proteins using mass spectrometryChristopher L Rector
Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045, USA
J Lipid Res 50:2064-71. 2009..It was determined that the S100A8/A9 protein complex protects LTA(4) from hydrolysis in a Ca(2+) dependent manner and increases LTA(4) half-life to in excess of 35 and 5 min at 4 degrees C and 37 degrees C, respectively... - Mammalian fatty acid synthase activity from crude tissue lysates tracing ¹³C-labeled substrates using gas chromatography-mass spectrometryMichael C Rudolph
Department of Pathology, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA
Anal Biochem 428:158-66. 2012..GC-MS quantification of [¹³C₁₆]palmitate synthesis permits sensitive evaluation of FASN activity from tissues of varied physiological states and of purified FASN activity in the presence of modifying proteins, enzymes, or drugs... - Sublimation as a method of matrix application for mass spectrometric imagingJoseph A Hankin
Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, Colorado 80045 0511, USA
J Am Soc Mass Spectrom 18:1646-52. 2007..This inexpensive method was reproducible and eliminated the potential for spreading of analytes arising from solvent deposition during matrix application... - Peroxide bond driven dissociation of hydroperoxy-cholesterol esters following collision induced dissociationPatrick M Hutchins
Department of Pharmacology, MSC 8303, University of Colorado Denver, RC1 South, L18 6120, 12801 E 17th Ave, P O Box 6511, Aurora, CO 80045, USA
J Am Soc Mass Spectrom 22:867-74. 2011.... - Specificity of acyl-homoserine lactone synthases examined by mass spectrometryTy A Gould
Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P.O. Box 8511 MS8303, Aurora CO 80045, USA
J Bacteriol 188:773-83. 2006..Furthermore, these studies offer additional methods that will be useful for surveying and quantitating AHLs from different sources... - Electrospray mass spectrometry of human hair wax estersMark Fitzgerald
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045 0511, USA
J Lipid Res 48:1231-46. 2007..The neutral loss experiment [loss of NH3 and CH2=CH-(CH2)nCH3] was particularly effective at rapidly surveying the complex biological mixture, identifying>160 different wax esters that range from 24 to 42 total carbons... - Direct qualitative analysis of triacylglycerols by electrospray mass spectrometry using a linear ion trapAndrew M McAnoy
Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado 80045-0511, USA
J Am Soc Mass Spectrom 16:1498-509. 2005..These strategies of MS, MS/MS, and MS3 experiments were applied to identify components within a complex mixture of neutral lipids extracted from RAW 264.7 cells... - Characterization of acrolein-glycerophosphoethanolamine lipid adducts using electrospray mass spectrometryKarin A Zemski Berry
Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
Chem Res Toxicol 20:1342-51. 2007.... - Formation of biologically active oxysterols during ozonolysis of cholesterol present in lung surfactantMelissa K Pulfer
Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80206, USA
J Biol Chem 279:26331-8. 2004..A possible mechanism for cytotoxicity is the ability of these oxysterols to inhibit isoprenoid-based cholesterol biosynthesis in these cells... - Covalent binding of leukotriene A4 to DNA and RNAJoseph A Hankin
Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, Colorado 80262, USA
Chem Res Toxicol 16:551-61. 2003.... - Free radical oxidation of plasmalogen glycerophosphocholine containing esterified docosahexaenoic acid: structure determination by mass spectrometryKarin A Zemski Berry
Cell Biology Division, Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80045-0511, USA
Antioxid Redox Signal 7:157-69. 2005..These results clearly indicate that free radical-induced oxidation of plasmalogen phospholipids with esterified docosahexaenoic acid at the sn-2 position underwent oxidation at both the sn-1 and sn-2 positions... - Analysis of cell membrane aminophospholipids as isotope-tagged derivativesKarin A Zemski Berry
Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
J Lipid Res 46:1038-46. 2005..7 cells to Cu2+/H2O2 to illustrate the ability of these reagents to aid in the mass spectrometric identification of aminophospholipid changes that occur during biological stimuli... - Cytochrome P-450 4F18 is the leukotriene B4 omega-1/omega-2 hydroxylase in mouse polymorphonuclear leukocytes: identification as the functional orthologue of human polymorphonuclear leukocyte CYP4F3A in the down-regulation of responses to LTB4Peter Christmas
Renal Unit and Department of Medicine, Massachusetts General Hospital East, Navy Yard Bldg 149, 13th Street, Charlestown, MA 02129, USA
J Biol Chem 281:7189-96. 2006..The results identify CYP4F18 as a critical protein in the regulation of LTB(4) metabolism and functional responses in mouse PMN and identify it as the functional orthologue of human PMN CYP4F3A... - Glutathione adducts of oxyeicosanoidsRobert C Murphy
Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
Prostaglandins Other Lipid Mediat 68:471-82. 2002..It has also been demonstrated that these oxyeicosanoid GSH-adducts do not represent just inactivation products, but they can both retain (GSH-adduct of hepoxilin A3) or show novel biological activities (LTC4 and FOG7)... - Ligand-receptor binding revealed by the TNF family member TALL-1Yingfang Liu
Integrated Department of Immunology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
Nature 423:49-56. 2003.... - Quantitation of cardiolipin molecular species in spontaneously hypertensive heart failure rats using electrospray ionization mass spectrometryGenevieve C Sparagna
Department of Integrative Physiology, University of Colorado Cardiovascular Institute, University of Colorado at Boulder, Boulder, CO 80309 0354, USA
J Lipid Res 46:1196-204. 2005..In animals with heart failure, the major cardiac CL species (tetralinoloyl) decreased significantly, whereas other minor CL species were significantly increased... - FcepsilonRI and Thy-1 domains have unique protein and lipid compositionsZurab Surviladze
Department of Pathology, University of New Mexico, Albuquerque, NM, USA
J Lipid Res 48:1325-35. 2007..5-3 times more abundant in FcRI domains than in the Thy-1 microdomains, whereas most diacyl GPE molecular species were equally abundant in the two domains... - Biosynthesis of 5-oxo-6,8,11,14-eicosatetraenoic acid from 5-hydroperoxyeicosatetraenoic acid in the murine macrophageSimona Zarini
Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
J Biol Chem 278:11190-6. 2003..Such a mechanism would enable racemic 5-HpETE, derived from free radical oxidation of arachidonic acid, to be efficiently converted into this potent chemotactic eicosanoid... - Direct electrospray tandem mass spectrometry of the unstable hydroperoxy bishemiacetal product derived from cholesterol ozonolysisMelissa K Pulfer
Department of Pediatrics, Division of Cell Biology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
J Am Soc Mass Spectrom 15:194-202. 2004.... - Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4Christian R H Raetz
Department of Biochemistry, Duke University Medical Center, Durham, NC, USA
J Lipid Res 47:1097-111. 2006..Kdo(2)-Lipid A is comparable to LPS by these criteria. Its activity is reduced by >10(3) in cells from TLR-4-deficient mice. The purity of Kdo(2)-Lipid A should facilitate structural analysis of complexes with receptors like TLR-4/MD2... - Pulmonary surfactant proteins and lipids as modulators of inflammation and innate immunityHirofumi Chiba
Program in Cell Biology, Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206, USA
Respirology 11:S2-6. 2006..CONCLUSIONS: These findings reveal that both the lipids and proteins of pulmonary surfactant play a role in regulating the host response to invading microorganisms... - Prostaglandin signaling in the renal collecting duct: release, reuptake, and oxidation in the same cellTeruhisa Nomura
Department of Medicine and Physiology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
J Biol Chem 280:28424-9. 2005..Our data are consistent with a new model that offers significant new mechanisms for the fine control of eicosanoid signaling... - Mass spectrometric analysis of leukotriene A4 and other chemically reactive metabolites of arachidonic acidJennifer S Dickinson
Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
J Am Soc Mass Spectrom 13:1227-34. 2002..The structures of these product ions were consistent with high resolution measurements measured in a quadrupole time-of-flight mass spectrometer... - Ultraviolet B radiation generates platelet-activating factor-like phospholipids underlying cutaneous damageGopal K Marathe
Human Molecular Biology and Genetics Program, University of Utah, Salt Lake City 84112 5330, USA
J Biol Chem 280:35448-57. 2005..PAF signaling may participate in the cutaneous inflammation that occurs during photo-aggravated dermatoses... - Oxidized phospholipids derived from ozone-treated lung surfactant extract reduce macrophage and epithelial cell viabilityCharis Uhlson
Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado 80206, USA
Chem Res Toxicol 15:896-906. 2002.... - Formation of murine macrophage-derived 5-oxo-7-glutathionyl-8,11,14-eicosatrienoic acid (FOG7) is catalyzed by leukotriene C4 synthaseJohn M Hevko
Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA
J Biol Chem 277:7037-43. 2002..These results suggest that LTC(4) synthase, thought to be specific for the conjugation of GSH to LTA(4), can also recognize 5-oxoETE as an electrophilic substrate... - Endotoxins stimulate neutrophil adhesion followed by synthesis and release of platelet-activating factor in microparticlesJunji Watanabe
Department of Human Molecular Biology and Genetics, University of Utah, Salt Lake City, Utah 84112-5330, USA
J Biol Chem 278:33161-8. 2003..Leukocytes can couple endotoxic challenge to the widespread circulatory and inflammatory effects of endotoxin... - Oxidized lipids formed non-enzymatically by reactive oxygen speciesWilliam L Smith
Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48108-0606, USA
J Biol Chem 283:15513-4. 2008 - Epidermal peroxisome proliferator-activated receptor gamma as a target for ultraviolet B radiationQiwei Zhang
Department of Dermatology, Indiana University School of Medicine, Indinapolis, Indiana 46202, USA
J Biol Chem 280:73-9. 2005..These studies demonstrated that epithelial cells contain a functional PPAR gamma system, and this system is a target for UVB through the production of novel oxidatively modified endogenous phospholipids... - On the biogenesis of lipid bodies in ancient eukaryotes: synthesis of triacylglycerols by a Toxoplasma DGAT1-related enzymeFriederike Quittnat
Department of Internal Medicine, Yale University School of Medicine, 808 LCI, 333 Cedar Street, P.O. Box 208022, New Haven, CT 06520, USA
Mol Biochem Parasitol 138:107-22. 2004.... - Linoleate-rich high-fat diet decreases mortality in hypertensive heart failure rats compared with lard and low-fat dietsAdam J Chicco
Department of Integrative Physiology, University of Colorado Cardiovascular Research Institute, University of Colorado, Boulder, USA
Hypertension 52:549-55. 2008..The precise mechanisms responsible for the divergent effects of the lard and linoleate-enriched diets merit further investigation but may involve diet-induced changes in the content and/or composition of cardiolipin in the heart... - Surfactant protein A binds Mycoplasma pneumoniae with high affinity and attenuates its growth by recognition of disaturated phosphatidylglycerolsSurapon Piboonpocanun
Program in Cell Biology, Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
J Biol Chem 280:9-17. 2005..pneumoniae by interacting with lipid ligands expressed on the surface of the organism and implicate SP-A in the immediate host response to the bacteria... - A comprehensive classification system for lipidsEoin Fahy
San Diego Supercomputer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0505, USA
J Lipid Res 46:839-61. 2005..This structured vocabulary will facilitate the systematization of lipid biology and enable the cataloging of lipids and their properties in a way that is compatible with other macromolecular databases... - Elucidation of double bond position in unsaturated lipids by ozone electrospray ionization mass spectrometryMichael C Thomas
Department of Chemistry, University of Wollongong, Wollongong NSW 2522, Australia
Anal Chem 79:5013-22. 2007..As such, OzESI-MS may provide important new insight into the molecular diversity of naturally occurring lipids... - Mass spectrometric quantitation of deoxyguanosine and leukotriene A4-deoxyguanosine adducts of DNAJoseph A Hankin
Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA
Anal Biochem 333:156-64. 2004.... - Recombinant human activated protein C reduces human endotoxin-induced pulmonary inflammation via inhibition of neutrophil chemotaxisJerry A Nick
Department of Medicine, National Jewish Medical and Research Center, D202, 1400 Jackson St, Denver, CO 80206, USA
Blood 104:3878-85. 2004..These rhAPC-induced effects on neutrophil function may represent a mechanism by which rhAPC improves survival in patients with sepsis... - Lysophospholipids of different classes mobilize neutrophil secretory vesicles and induce redundant signaling through G2AS Courtney Frasch
Department of Pediatrics, Division of Cell Biology, National Jewish Medical and Research Center, Denver, CO 80206, USA
J Immunol 178:6540-8. 2007..Together, these data show that lyso-PLs bearing various head groups redundantly mobilize G2A latent within secretory vesicles and result in G2A receptor/Galphai/phospholipase C signaling for calcium flux in neutrophils... - Ozone exposure in vivo and formation of biologically active oxysterols in the lungMelissa K Pulfer
Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado, USA
J Pharmacol Exp Ther 312:256-64. 2005..The specific detection of cholestan-6-oxo-3,5-diol in lung homogenate after ozone exposure established formation of 5beta,6beta-epoxycholesterol within the lung after exposure to 0.5 ppm ozone... - Electrospray ionization tandem mass spectrometry of glycerophosphoethanolamine plasmalogen phospholipidsKarin A Zemski Berry
Department of Pediatrics, Cell Biology Division, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
J Am Soc Mass Spectrom 15:1499-508. 2004..These two ions were used to detect specific molecular species of GPEtn containing esterified arachidonate (precursors of m/z 361) present in the human neutrophil... - Cysteinyl-leukotrienes receptor activation in brain inflammatory reactions and cerebral edema formation: a role for transcellular biosynthesis of cysteinyl-leukotrienesAntonio Di Gennaro
Center for Cardiopulmonary Pharmacology and Department of Pharmacological Sciences, University of Milan, Milan, Italy
FASEB J 18:842-4. 2004.... - Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylationWayne R Riekhof
Department of Medicine, Program in Cell Biology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
J Biol Chem 282:36853-61. 2007..These results establish a new pathway for the net synthesis of PtdCho in yeast and provide new tools for the study of PtdCho synthesis, transport, and remodeling...
Research Grants
- LEUKOTRIENES AND SLOW REACTING SUBSTANCE OF ANAPHYLAXISRobert Murphy; Fiscal Year: 2007..Mixture of macrophage or neutrophils deficient in leukotriene cascade enzymes will be stimulated and products studied in studies of transcellular biosynthesis in specific cell types. ..