Robert C Murphy

Summary

Affiliation: University of Colorado Health Sciences Center
Country: USA

Publications

  1. ncbi request reprint Electrospray mass spectrometry of phospholipids
    Melissa Pulfer
    Department of Pediatrics, Division of Cell Biology, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado 80206, USA
    Mass Spectrom Rev 22:332-64. 2003
  2. ncbi request reprint Electrospray ionization and tandem mass spectrometry of eicosanoids
    Robert C Murphy
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Mail Stop 8303, P O Box 6511, Aurora, CO 80045 0511, USA
    Anal Biochem 346:1-42. 2005
  3. pmc Cholesterol, reactive oxygen species, and the formation of biologically active mediators
    Robert C Murphy
    Department of Pharmacology, University of Colorado Denver Health Sciences Center, Aurora, Colorado 80045 6511, USA
    J Biol Chem 283:15521-5. 2008
  4. pmc Detection of the abundance of diacylglycerol and triacylglycerol molecular species in cells using neutral loss mass spectrometry
    Robert C Murphy
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA
    Anal Biochem 366:59-70. 2007
  5. pmc The 5-lipoxygenase pathway is required for acute lung injury following hemorrhagic shock
    John C Eun
    Department of Surgery, University of Colorado Denver, Aurora, Colorado 80204, USA
    Shock 37:599-604. 2012
  6. ncbi request reprint Characterization of oxidized phosphatidylethanolamine derived from RAW 264.7 cells using 4-(dimethylamino)benzoic acid derivatives
    Karin A Zemski Berry
    Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045, USA
    Eur J Mass Spectrom (Chichester, Eng) 16:463-70. 2010
  7. pmc MALDI imaging MS of phospholipids in the mouse lung
    Karin A Zemski Berry
    Department of Pharmacology, University of Colorado Denver, 12801 East 17 Avenue, Mail Stop 8303, Aurora, CO 80045, USA
    J Lipid Res 52:1551-60. 2011
  8. ncbi request reprint Qualitative analysis and quantitative assessment of changes in neutral glycerol lipid molecular species within cells
    Jessica Krank
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado
    Methods Enzymol 432:1-20. 2007
  9. ncbi request reprint Blocking leukotriene synthesis attenuates the pathophysiology of traumatic brain injury and associated cognitive deficits
    Chelsea E Corser-Jensen
    Neuroscience Program, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA Department of Pharmacology, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA
    Exp Neurol 256:7-16. 2014
  10. doi request reprint Lipid mediators in cerebral spinal fluid of traumatic brain injured patients
    Santiago E Farias
    Department of Pharmacology, University of Colorado at Denver, Anschutz Medical Campus, Aurora, CO, USA
    J Trauma 71:1211-8. 2011

Research Grants

Collaborators

Detail Information

Publications82

  1. ncbi request reprint Electrospray mass spectrometry of phospholipids
    Melissa Pulfer
    Department of Pediatrics, Division of Cell Biology, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado 80206, USA
    Mass Spectrom Rev 22:332-64. 2003
    ..Several applications of mass spectrometry applied to phospholipid analysis are presented as they apply to physiology as well as pathophysiology...
  2. ncbi request reprint Electrospray ionization and tandem mass spectrometry of eicosanoids
    Robert C Murphy
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Mail Stop 8303, P O Box 6511, Aurora, CO 80045 0511, USA
    Anal Biochem 346:1-42. 2005
  3. pmc Cholesterol, reactive oxygen species, and the formation of biologically active mediators
    Robert C Murphy
    Department of Pharmacology, University of Colorado Denver Health Sciences Center, Aurora, Colorado 80045 6511, USA
    J Biol Chem 283:15521-5. 2008
  4. pmc Detection of the abundance of diacylglycerol and triacylglycerol molecular species in cells using neutral loss mass spectrometry
    Robert C Murphy
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA
    Anal Biochem 366:59-70. 2007
    ..A 50:1-TAG containing 18:1 was found to increase significantly over a 24-h time course after Kdo(2)-lipid A exposure, whereas an isobaric 50:1-TAG containing 16:1 did not change relative to controls...
  5. pmc The 5-lipoxygenase pathway is required for acute lung injury following hemorrhagic shock
    John C Eun
    Department of Surgery, University of Colorado Denver, Aurora, Colorado 80204, USA
    Shock 37:599-604. 2012
    ..This pathway could represent a new target for pharmacological intervention to reduce lung damage following severe primary injury...
  6. ncbi request reprint Characterization of oxidized phosphatidylethanolamine derived from RAW 264.7 cells using 4-(dimethylamino)benzoic acid derivatives
    Karin A Zemski Berry
    Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045, USA
    Eur J Mass Spectrom (Chichester, Eng) 16:463-70. 2010
    ..The stable isotope labeled derivatives permitted assessment of relative quantitative changes in oxidized lipids based upon ion abundance...
  7. pmc MALDI imaging MS of phospholipids in the mouse lung
    Karin A Zemski Berry
    Department of Pharmacology, University of Colorado Denver, 12801 East 17 Avenue, Mail Stop 8303, Aurora, CO 80045, USA
    J Lipid Res 52:1551-60. 2011
    ..This technique revealed unexpected high concentrations of arachidonate- and docosahexaenoate-containing phospholipids lining the airways in pulmonary tissue, which could serve as precursors of lipid mediators affecting airways biology...
  8. ncbi request reprint Qualitative analysis and quantitative assessment of changes in neutral glycerol lipid molecular species within cells
    Jessica Krank
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado
    Methods Enzymol 432:1-20. 2007
    ..An example of the application of this method was in the quantitative analysis of TAG and DAG molecular species present in RAW 264.7 cells treated with a Toll-4 receptor ligand, Kdo2-lipid A, in a time course study...
  9. ncbi request reprint Blocking leukotriene synthesis attenuates the pathophysiology of traumatic brain injury and associated cognitive deficits
    Chelsea E Corser-Jensen
    Neuroscience Program, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA Department of Pharmacology, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA
    Exp Neurol 256:7-16. 2014
    ..FLAP inhibitors represent a novel anti-inflammatory approach for treating human TBI that is feasible for both intervention and prevention of brain injury and neurologic deficits. ..
  10. doi request reprint Lipid mediators in cerebral spinal fluid of traumatic brain injured patients
    Santiago E Farias
    Department of Pharmacology, University of Colorado at Denver, Anschutz Medical Campus, Aurora, CO, USA
    J Trauma 71:1211-8. 2011
    ..Thus, levels of these metabolites in normal and pathologic conditions in the brain are particularly relevant in understanding the transition to disease...
  11. ncbi request reprint Biosynthesis of eicosanoids and transcellular metabolism of leukotrienes in murine bone marrow cells
    Miguel A Gijón
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045 6511, USA
    J Lipid Res 48:716-25. 2007
    ..These data demonstrate that murine bone marrow PMNs are a valid model for the study of LT biosynthesis, which now offers the possibility to investigate specific biochemical pathways through the use of transgenic mice...
  12. pmc Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspI
    J Paul Kirwan
    Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P O Box 8511 MS8303, Aurora, CO 80045, USA
    J Bacteriol 188:784-8. 2006
    ..Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone...
  13. pmc Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils
    Miguel A Gijón
    Department of Pharmacology, University of Colorado Denver, Aurora, Colorado 80045, USA
    J Biol Chem 283:30235-45. 2008
    ..These results strongly implicate MBOAT5 and MBOAT7 in arachidonate recycling, thus regulating free arachidonic acid levels and leukotriene synthesis in neutrophils...
  14. pmc Transcellular biosynthesis of cysteinyl leukotrienes in vivo during mouse peritoneal inflammation
    Simona Zarini
    Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045, USA
    Proc Natl Acad Sci U S A 106:8296-301. 2009
    ..These results highlight the potential relevance of transcellular exchange of LTA(4) for the synthesis of LTs mediating biological activities during inflammatory events in vivo...
  15. pmc Effects of acrolein on leukotriene biosynthesis in human neutrophils
    Karin A Zemski Berry
    Department of Pharmacology, MSC 8303, University of Colorado Denver, RC1 South, L18 6120, 12801 East 17th Avenue, P O Box 6511, Aurora, Colorado 80045, USA
    Chem Res Toxicol 21:2424-32. 2008
    ....
  16. doi request reprint MALDI mass spectrometric imaging of lipids in rat brain injury models
    Joseph A Hankin
    Department of Pharmacology, University of Colorado Denver, Aurora, CO, USA
    J Am Soc Mass Spectrom 22:1014-21. 2011
    ..These studies reveal the value of MALDI IMS to examine tissues for changes in lipid biochemistry and will provide data needed to eventually understand the biochemical mechanisms relevant to tissue injury...
  17. pmc Injury-related production of cysteinyl leukotrienes contributes to brain damage following experimental traumatic brain injury
    Santiago Farias
    Department of Pharmacology, University of Colorado at Denver, Anschutz Medical Campus, Aurora, Colorado 80045, USA
    J Neurotrauma 26:1977-86. 2009
    ....
  18. ncbi request reprint Transcellular biosynthesis of cysteinyl leukotrienes in rat neuronal and glial cells
    Santiago E Farias
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado, and the Denver VA Medical Center, Denver, Colorado, USA
    J Neurochem 103:1310-8. 2007
    ..The formation of LTC(4) through this mechanism may be relevant in the genesis and progression of the inflammatory response as a result of brain injury...
  19. ncbi request reprint Eicosanoid transcellular biosynthesis: from cell-cell interactions to in vivo tissue responses
    Giancarlo Folco
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Mail Stop 8303, 12801 E 17th Avenue, P O Box 6511, Aurora, CO 80045 0511, USA
    Pharmacol Rev 58:375-88. 2006
    ..Experiments for transcellular events taking place in vivo that reveal the true complexity of eicosanoid biosynthesis within tissues are also reviewed...
  20. pmc Mammalian fatty acid synthase activity from crude tissue lysates tracing ¹³C-labeled substrates using gas chromatography-mass spectrometry
    Michael C Rudolph
    Department of Pathology, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA
    Anal Biochem 428:158-66. 2012
    ..GC-MS quantification of [¹³C₁₆]palmitate synthesis permits sensitive evaluation of FASN activity from tissues of varied physiological states and of purified FASN activity in the presence of modifying proteins, enzymes, or drugs...
  21. pmc MALDI imaging of lipids after matrix sublimation/deposition
    Robert C Murphy
    Department of Pharmacology, University of Colorado, Denver, CO, USA
    Biochim Biophys Acta 1811:970-5. 2011
    ..Specific examples of sublimation/deposition in lipid imaging of various organs including brain, ocular tissue, and kidney are presented...
  22. pmc Formation of eicosanoids, E2/D2 isoprostanes, and docosanoids following decapitation-induced ischemia, measured in high-energy-microwaved rat brain
    Santiago E Farias
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
    J Lipid Res 49:1990-2000. 2008
    ..The present study demonstrated increased formation of eicosanoids, E(2)/D(2)-IsoPs, and docosanoids following cerebral ischemia. A balance of these lipid mediators may mediate immediate events of ischemic injury and recovery...
  23. ncbi request reprint Biosynthesis and metabolism of leukotrienes
    Robert C Murphy
    Department of Pharmacology, Mail Stop 8303, University of Colorado at Denver and Health Sciences Center, 12801 E 17th Avenue, P O Box 6511, Aurora, CO 80045 0511, USA
    Biochem J 405:379-95. 2007
    ..These metabolic transformations of the primary leukotrienes are critical for termination of their biological activity, and defects in expression of participating enzymes may be involved in specific genetic disease...
  24. pmc Separation of cellular nonpolar neutral lipids by normal-phase chromatography and analysis by electrospray ionization mass spectrometry
    Patrick M Hutchins
    Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045 6511, USA
    J Lipid Res 49:804-13. 2008
    ..Longer chain CE molecular species did not change in response to the activation of these cells...
  25. pmc Imaging of lipid species by MALDI mass spectrometry
    Robert C Murphy
    Department of Pharmacology, MS 8303 University of Colorado Denver, Aurora, CO 80045, USA
    J Lipid Res 50:S317-22. 2009
    ..Regional distribution of many different lipid molecular species and Na+ and K+ attachment ions often define anatomical regions within the tissues...
  26. pmc Low-concentration ozone reacts with plasmalogen glycerophosphoethanolamine lipids in lung surfactant
    Kelly M Wynalda
    Department of Pharmacology, University of Colorado Denver, MS 8303, 12801 E 17th Ave, Aurora, Colorado 80045, USA
    Chem Res Toxicol 23:108-17. 2010
    ..Lysophospholipids, pentadecanal, and nonanal were found to be the primary products of surfactant ozone oxidation...
  27. ncbi request reprint Lipid rafts regulate lipopolysaccharide-induced activation of Cdc42 and inflammatory functions of the human neutrophil
    Michael B Fessler
    Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 279:39989-98. 2004
    ..In sum, these findings provide evidence for an important regulatory role of cholesterol in both transmission of the lipopolysaccharide signal and the inflammatory phenotype of the human neutrophil...
  28. pmc Mechanism of formation of the major estradiol product ions following collisional activation of the molecular anion in a tandem quadrupole mass spectrometer
    Kerry M Wooding
    Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO, 80045, USA
    J Am Soc Mass Spectrom 24:1451-5. 2013
    ..We propose two fragmentation schemes to explain the CID data and suggest a structure of m/z 183 and m/z 169 consistent with several isotopic variants and high resolution mass spectrometric measurements. ..
  29. pmc Cholesteryl ester acyl oxidation and remodeling in murine macrophages: formation of oxidized phosphatidylcholine
    Patrick M Hutchins
    Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045, USA
    J Lipid Res 53:1588-97. 2012
    ..Further experiments revealed these oxPC species were formed chiefly from the hydrolysis of oxidized CE and subsequent reacylation of the oxidized acyl components into PC...
  30. pmc Electrospray MS/MS reveals extensive and nonspecific oxidation of cholesterol esters in human peripheral vascular lesions
    Patrick M Hutchins
    Department of Pharmacology, University of Colorado at Denver, Aurora, CO 80045, USA
    J Lipid Res 52:2070-83. 2011
    ....
  31. ncbi request reprint Effect of arachidonic acid reacylation on leukotriene biosynthesis in human neutrophils stimulated with granulocyte-macrophage colony-stimulating factor and formyl-methionyl-leucyl-phenylalanine
    Simona Zarini
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, 12801 E 17th Avenue, Aurora, CO 80045, USA
    J Biol Chem 281:10134-42. 2006
    ....
  32. pmc Relationship between MALDI IMS intensity and measured quantity of selected phospholipids in rat brain sections
    Joseph A Hankin
    Department of Pharmacology, University of Colorado Denver, Mail Stop 8303, Aurora, Colorado 80045, USA
    Anal Chem 82:8476-84. 2010
    ..There was good correlation between the MALDI IMS ion abundance of PC molecular species and the relative abundance of corresponding PC molecular species in microdissected regions analyzed by LC/MS/MS...
  33. pmc Quantitative analysis of phospholipids containing arachidonate and docosahexaenoate chains in microdissected regions of mouse brain
    Paul H Axelsen
    Department of Pharmacology, Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    J Lipid Res 51:660-71. 2010
    ..These differences may be related to the differential susceptibility of different brain regions to neurodegenerative disorders...
  34. pmc Stable isotope labeled 4-(dimethylamino)benzoic acid derivatives of glycerophosphoethanolamine lipids
    Karin A Zemski Berry
    Department of Pharmacology, University of Colorado Denver, Aurora, Colorado 80045, USA
    Anal Chem 81:6633-40. 2009
    ..These DMABA NHS ester reagents provide a universal scan for diacyl, ether, and plasmalogen PE lipids that cannot be readily observed otherwise, enable differential labeling, and provide an internal standard for each PE lipid...
  35. ncbi request reprint Mass spectrometry advances in lipidomica: collision-induced decomposition of Kdo2-lipid A
    Robert C Murphy
    Department of Pharmacology, University of Colorado Health Sciences Center, Campus Box C236, 4200 E 9th Avenue, Denver, CO 80262, USA
    Prostaglandins Other Lipid Mediat 77:131-40. 2005
    ..A rather different challenge is that for the analysis of abundant lipid classes, which are composed of multiple molecular species that can approach several hundred under certain circumstances...
  36. pmc Measurement of lysophospholipid acyltransferase activities using substrate competition
    Sarah A Martin
    Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045
    J Lipid Res 55:782-91. 2014
    ..7 cells was easily detected by the dual choice method. These findings demonstrate that this assay is both specific and sensitive and that it provides much richer biochemical detail than traditional assays. ..
  37. pmc Group IVA phospholipase A₂ is necessary for growth cone repulsion and collapse
    Staci D Sanford
    Department of Pediatrics, Neuroscience Program, and Colorado Intellectual and Developmental Disabilities Research Center, University of Colorado School of Medicine, Aurora, Colorado, USA
    J Neurochem 120:974-84. 2012
    ..These studies identify a novel, early step in Sema3A-signaling and a PLA(2) necessary for growth cone repulsion and collapse...
  38. pmc Mass spectrometric analysis of long-chain lipids
    Robert C Murphy
    Department of Pharmacology, University of Colorado Denver, Aurora, USA
    Mass Spectrom Rev 30:579-99. 2011
    ....
  39. pmc Working towards an exegesis for lipids in biology
    H Alex Brown
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Nat Chem Biol 5:602-6. 2009
    ....
  40. pmc New applications of mass spectrometry in lipid analysis
    Robert C Murphy
    Department of Pharmacology, University of Colorado Denver, Aurora, Colorado 80045, USA
    J Biol Chem 286:25427-33. 2011
    ..Imaging mass spectrometry of lipids in tissues has opened new insights into the distribution of lipid molecular species with promising application to study pathophysiological events and diseases...
  41. pmc Glycerolipid and cholesterol ester analyses in biological samples by mass spectrometry
    Robert C Murphy
    Department of Pharmacology, University of Colorado, Denver, CO, USA
    Biochim Biophys Acta 1811:776-83. 2011
    ..Several applications of these approaches are discussed. ...
  42. pmc Determination of leukotriene A(4) stabilization by S100A8/A9 proteins using mass spectrometry
    Christopher L Rector
    Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045, USA
    J Lipid Res 50:2064-71. 2009
    ..It was determined that the S100A8/A9 protein complex protects LTA(4) from hydrolysis in a Ca(2+) dependent manner and increases LTA(4) half-life to in excess of 35 and 5 min at 4 degrees C and 37 degrees C, respectively...
  43. pmc Spatial organization of lipids in the human retina and optic nerve by MALDI imaging mass spectrometry
    Karin A Zemski Berry
    Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045 and
    J Lipid Res 55:504-15. 2014
    ....
  44. pmc Specificity of acyl-homoserine lactone synthases examined by mass spectrometry
    Ty A Gould
    Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P O Box 8511 MS8303, Aurora CO 80045, USA
    J Bacteriol 188:773-83. 2006
    ..Furthermore, these studies offer additional methods that will be useful for surveying and quantitating AHLs from different sources...
  45. pmc Characterization of acrolein-glycerophosphoethanolamine lipid adducts using electrospray mass spectrometry
    Karin A Zemski Berry
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
    Chem Res Toxicol 20:1342-51. 2007
    ....
  46. pmc Sublimation as a method of matrix application for mass spectrometric imaging
    Joseph A Hankin
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, Colorado 80045 0511, USA
    J Am Soc Mass Spectrom 18:1646-52. 2007
    ..This inexpensive method was reproducible and eliminated the potential for spreading of analytes arising from solvent deposition during matrix application...
  47. pmc Peroxide bond driven dissociation of hydroperoxy-cholesterol esters following collision induced dissociation
    Patrick M Hutchins
    Department of Pharmacology, MSC 8303, University of Colorado Denver, RC1 South, L18 6120, 12801 E 17th Ave, P O Box 6511, Aurora, CO 80045, USA
    J Am Soc Mass Spectrom 22:867-74. 2011
    ....
  48. ncbi request reprint Direct qualitative analysis of triacylglycerols by electrospray mass spectrometry using a linear ion trap
    Andrew M McAnoy
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado 80045 0511, USA
    J Am Soc Mass Spectrom 16:1498-509. 2005
    ..These strategies of MS, MS/MS, and MS3 experiments were applied to identify components within a complex mixture of neutral lipids extracted from RAW 264.7 cells...
  49. ncbi request reprint Electrospray mass spectrometry of human hair wax esters
    Mark Fitzgerald
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045 0511, USA
    J Lipid Res 48:1231-46. 2007
    ..The neutral loss experiment [loss of NH3 and CH2=CH-(CH2)nCH3] was particularly effective at rapidly surveying the complex biological mixture, identifying>160 different wax esters that range from 24 to 42 total carbons...
  50. ncbi request reprint Covalent binding of leukotriene A4 to DNA and RNA
    Joseph A Hankin
    Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, Colorado 80262, USA
    Chem Res Toxicol 16:551-61. 2003
    ....
  51. ncbi request reprint Formation of biologically active oxysterols during ozonolysis of cholesterol present in lung surfactant
    Melissa K Pulfer
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80206, USA
    J Biol Chem 279:26331-8. 2004
    ..A possible mechanism for cytotoxicity is the ability of these oxysterols to inhibit isoprenoid-based cholesterol biosynthesis in these cells...
  52. ncbi request reprint Analysis of cell membrane aminophospholipids as isotope-tagged derivatives
    Karin A Zemski Berry
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
    J Lipid Res 46:1038-46. 2005
    ..7 cells to Cu2+/H2O2 to illustrate the ability of these reagents to aid in the mass spectrometric identification of aminophospholipid changes that occur during biological stimuli...
  53. ncbi request reprint Free radical oxidation of plasmalogen glycerophosphocholine containing esterified docosahexaenoic acid: structure determination by mass spectrometry
    Karin A Zemski Berry
    Cell Biology Division, Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80045 0511, USA
    Antioxid Redox Signal 7:157-69. 2005
    ..These results clearly indicate that free radical-induced oxidation of plasmalogen phospholipids with esterified docosahexaenoic acid at the sn-2 position underwent oxidation at both the sn-1 and sn-2 positions...
  54. ncbi request reprint Glutathione adducts of oxyeicosanoids
    Robert C Murphy
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    Prostaglandins Other Lipid Mediat 68:471-82. 2002
    ..It has also been demonstrated that these oxyeicosanoid GSH-adducts do not represent just inactivation products, but they can both retain (GSH-adduct of hepoxilin A3) or show novel biological activities (LTC4 and FOG7)...
  55. ncbi request reprint Cytochrome P-450 4F18 is the leukotriene B4 omega-1/omega-2 hydroxylase in mouse polymorphonuclear leukocytes: identification as the functional orthologue of human polymorphonuclear leukocyte CYP4F3A in the down-regulation of responses to LTB4
    Peter Christmas
    Renal Unit and Department of Medicine, Massachusetts General Hospital East, Navy Yard Bldg 149, 13th Street, Charlestown, MA 02129, USA
    J Biol Chem 281:7189-96. 2006
    ..The results identify CYP4F18 as a critical protein in the regulation of LTB(4) metabolism and functional responses in mouse PMN and identify it as the functional orthologue of human PMN CYP4F3A...
  56. ncbi request reprint Biosynthesis of 5-oxo-6,8,11,14-eicosatetraenoic acid from 5-hydroperoxyeicosatetraenoic acid in the murine macrophage
    Simona Zarini
    Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 278:11190-6. 2003
    ..Such a mechanism would enable racemic 5-HpETE, derived from free radical oxidation of arachidonic acid, to be efficiently converted into this potent chemotactic eicosanoid...
  57. ncbi request reprint Ligand-receptor binding revealed by the TNF family member TALL-1
    Yingfang Liu
    Integrated Department of Immunology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    Nature 423:49-56. 2003
    ....
  58. ncbi request reprint Cysteinyl-leukotrienes receptor activation in brain inflammatory reactions and cerebral edema formation: a role for transcellular biosynthesis of cysteinyl-leukotrienes
    Antonio Di Gennaro
    Center for Cardiopulmonary Pharmacology and Department of Pharmacological Sciences, University of Milan, Milan, Italy
    FASEB J 18:842-4. 2004
    ....
  59. ncbi request reprint Mass spectrometric analysis of leukotriene A4 and other chemically reactive metabolites of arachidonic acid
    Jennifer S Dickinson
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Am Soc Mass Spectrom 13:1227-34. 2002
    ..The structures of these product ions were consistent with high resolution measurements measured in a quadrupole time-of-flight mass spectrometer...
  60. ncbi request reprint A comprehensive classification system for lipids
    Eoin Fahy
    San Diego Supercomputer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0505, USA
    J Lipid Res 46:839-61. 2005
    ..This structured vocabulary will facilitate the systematization of lipid biology and enable the cataloging of lipids and their properties in a way that is compatible with other macromolecular databases...
  61. ncbi request reprint Oxidized phospholipids derived from ozone-treated lung surfactant extract reduce macrophage and epithelial cell viability
    Charis Uhlson
    Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado 80206, USA
    Chem Res Toxicol 15:896-906. 2002
    ....
  62. ncbi request reprint Endotoxins stimulate neutrophil adhesion followed by synthesis and release of platelet-activating factor in microparticles
    Junji Watanabe
    Department of Human Molecular Biology and Genetics, University of Utah, Salt Lake City, Utah 84112 5330, USA
    J Biol Chem 278:33161-8. 2003
    ..Leukocytes can couple endotoxic challenge to the widespread circulatory and inflammatory effects of endotoxin...
  63. ncbi request reprint Lysophospholipids of different classes mobilize neutrophil secretory vesicles and induce redundant signaling through G2A
    S Courtney Frasch
    Department of Pediatrics, Division of Cell Biology, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Immunol 178:6540-8. 2007
    ..Together, these data show that lyso-PLs bearing various head groups redundantly mobilize G2A latent within secretory vesicles and result in G2A receptor/Galphai/phospholipase C signaling for calcium flux in neutrophils...
  64. ncbi request reprint Epidermal peroxisome proliferator-activated receptor gamma as a target for ultraviolet B radiation
    Qiwei Zhang
    Department of Dermatology, Indiana University School of Medicine, Indinapolis, Indiana 46202, USA
    J Biol Chem 280:73-9. 2005
    ..These studies demonstrated that epithelial cells contain a functional PPAR gamma system, and this system is a target for UVB through the production of novel oxidatively modified endogenous phospholipids...
  65. ncbi request reprint FcepsilonRI and Thy-1 domains have unique protein and lipid compositions
    Zurab Surviladze
    Department of Pathology, University of New Mexico, Albuquerque, NM, USA
    J Lipid Res 48:1325-35. 2007
    ..5-3 times more abundant in FcRI domains than in the Thy-1 microdomains, whereas most diacyl GPE molecular species were equally abundant in the two domains...
  66. ncbi request reprint Formation of murine macrophage-derived 5-oxo-7-glutathionyl-8,11,14-eicosatrienoic acid (FOG7) is catalyzed by leukotriene C4 synthase
    John M Hevko
    Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA
    J Biol Chem 277:7037-43. 2002
    ..These results suggest that LTC(4) synthase, thought to be specific for the conjugation of GSH to LTA(4), can also recognize 5-oxoETE as an electrophilic substrate...
  67. ncbi request reprint Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4
    Christian R H Raetz
    Department of Biochemistry, Duke University Medical Center, Durham, NC, USA
    J Lipid Res 47:1097-111. 2006
    ..Kdo(2)-Lipid A is comparable to LPS by these criteria. Its activity is reduced by >10(3) in cells from TLR-4-deficient mice. The purity of Kdo(2)-Lipid A should facilitate structural analysis of complexes with receptors like TLR-4/MD2...
  68. ncbi request reprint Pulmonary surfactant proteins and lipids as modulators of inflammation and innate immunity
    Hirofumi Chiba
    Program in Cell Biology, Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206, USA
    Respirology 11:S2-6. 2006
    ..The authors examined the interaction of SP-A with Mycoplasma pneumoniae and the influence of the protein upon the innate immune response to the bacteria...
  69. ncbi request reprint Ultraviolet B radiation generates platelet-activating factor-like phospholipids underlying cutaneous damage
    Gopal K Marathe
    Human Molecular Biology and Genetics Program, University of Utah, Salt Lake City 84112 5330, USA
    J Biol Chem 280:35448-57. 2005
    ..PAF signaling may participate in the cutaneous inflammation that occurs during photo-aggravated dermatoses...
  70. ncbi request reprint Quantitation of cardiolipin molecular species in spontaneously hypertensive heart failure rats using electrospray ionization mass spectrometry
    Genevieve C Sparagna
    Department of Integrative Physiology, University of Colorado Cardiovascular Institute, University of Colorado at Boulder, Boulder, CO 80309 0354, USA
    J Lipid Res 46:1196-204. 2005
    ..In animals with heart failure, the major cardiac CL species (tetralinoloyl) decreased significantly, whereas other minor CL species were significantly increased...
  71. ncbi request reprint On the biogenesis of lipid bodies in ancient eukaryotes: synthesis of triacylglycerols by a Toxoplasma DGAT1-related enzyme
    Friederike Quittnat
    Department of Internal Medicine, Yale University School of Medicine, 808 LCI, 333 Cedar Street, P O Box 208022, New Haven, CT 06520, USA
    Mol Biochem Parasitol 138:107-22. 2004
    ....
  72. ncbi request reprint Surfactant protein A binds Mycoplasma pneumoniae with high affinity and attenuates its growth by recognition of disaturated phosphatidylglycerols
    Surapon Piboonpocanun
    Program in Cell Biology, Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 280:9-17. 2005
    ..pneumoniae by interacting with lipid ligands expressed on the surface of the organism and implicate SP-A in the immediate host response to the bacteria...
  73. ncbi request reprint Electrospray ionization tandem mass spectrometry of glycerophosphoethanolamine plasmalogen phospholipids
    Karin A Zemski Berry
    Department of Pediatrics, Cell Biology Division, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Am Soc Mass Spectrom 15:1499-508. 2004
    ..These two ions were used to detect specific molecular species of GPEtn containing esterified arachidonate (precursors of m/z 361) present in the human neutrophil...
  74. ncbi request reprint Direct electrospray tandem mass spectrometry of the unstable hydroperoxy bishemiacetal product derived from cholesterol ozonolysis
    Melissa K Pulfer
    Department of Pediatrics, Division of Cell Biology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Am Soc Mass Spectrom 15:194-202. 2004
    ....
  75. ncbi request reprint Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation
    Wayne R Riekhof
    Department of Medicine, Program in Cell Biology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 282:36853-61. 2007
    ..These results establish a new pathway for the net synthesis of PtdCho in yeast and provide new tools for the study of PtdCho synthesis, transport, and remodeling...
  76. pmc Linoleate-rich high-fat diet decreases mortality in hypertensive heart failure rats compared with lard and low-fat diets
    Adam J Chicco
    Department of Integrative Physiology, University of Colorado Cardiovascular Research Institute, University of Colorado, Boulder, USA
    Hypertension 52:549-55. 2008
    ..The precise mechanisms responsible for the divergent effects of the lard and linoleate-enriched diets merit further investigation but may involve diet-induced changes in the content and/or composition of cardiolipin in the heart...
  77. pmc Elucidation of double bond position in unsaturated lipids by ozone electrospray ionization mass spectrometry
    Michael C Thomas
    Department of Chemistry, University of Wollongong, Wollongong NSW 2522, Australia
    Anal Chem 79:5013-22. 2007
    ..As such, OzESI-MS may provide important new insight into the molecular diversity of naturally occurring lipids...
  78. ncbi request reprint Ozone exposure in vivo and formation of biologically active oxysterols in the lung
    Melissa K Pulfer
    Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado, USA
    J Pharmacol Exp Ther 312:256-64. 2005
    ..The specific detection of cholestan-6-oxo-3,5-diol in lung homogenate after ozone exposure established formation of 5beta,6beta-epoxycholesterol within the lung after exposure to 0.5 ppm ozone...
  79. pmc Oxidized lipids formed non-enzymatically by reactive oxygen species
    William L Smith
    Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48108 0606, USA
    J Biol Chem 283:15513-4. 2008
  80. ncbi request reprint Recombinant human activated protein C reduces human endotoxin-induced pulmonary inflammation via inhibition of neutrophil chemotaxis
    Jerry A Nick
    Department of Medicine, National Jewish Medical and Research Center, D202, 1400 Jackson St, Denver, CO 80206, USA
    Blood 104:3878-85. 2004
    ..These rhAPC-induced effects on neutrophil function may represent a mechanism by which rhAPC improves survival in patients with sepsis...
  81. ncbi request reprint Mass spectrometric quantitation of deoxyguanosine and leukotriene A4-deoxyguanosine adducts of DNA
    Joseph A Hankin
    Division of Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA
    Anal Biochem 333:156-64. 2004
    ....
  82. ncbi request reprint Prostaglandin signaling in the renal collecting duct: release, reuptake, and oxidation in the same cell
    Teruhisa Nomura
    Department of Medicine and Physiology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 280:28424-9. 2005
    ..Our data are consistent with a new model that offers significant new mechanisms for the fine control of eicosanoid signaling...

Research Grants1

  1. LEUKOTRIENES AND SLOW REACTING SUBSTANCE OF ANAPHYLAXIS
    Robert Murphy; Fiscal Year: 2007
    ..Mixture of macrophage or neutrophils deficient in leukotriene cascade enzymes will be stimulated and products studied in studies of transcellular biosynthesis in specific cell types. ..