Kenan C Murphy

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. doi request reprint Rapid allelic exchange in enterohemorrhagic Escherichia coli (EHEC) and other E. coli using lambda red recombination
    Pamela J Savage
    University of Massachusetts MedicalSchool, Worcester, Massachusetts, USA
    Curr Protoc Microbiol . 2006
  2. doi request reprint Phage recombinases and their applications
    Kenan C Murphy
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA, USA
    Adv Virus Res 83:367-414. 2012
  3. ncbi request reprint PCR-mediated gene replacement in Escherichia coli
    K C Murphy
    Department of Molecular Genetics and Microbiology University of Massachusetts Medical School, Worcester, MA 01655, USA
    Gene 246:321-30. 2000
  4. doi request reprint Targeted chromosomal gene knockout using PCR fragments
    Kenan C Murphy
    Department of Microbial and Physiological systems, University of Massachusetts Medical School, Worcester, MA, USA
    Methods Mol Biol 765:27-42. 2011
  5. pmc Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli
    Kenan C Murphy
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    BMC Mol Biol 4:11. 2003
  6. ncbi request reprint The lambda Gam protein inhibits RecBCD binding to dsDNA ends
    Kenan C Murphy
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue, North, Worcester, MA 01655, USA
    J Mol Biol 371:19-24. 2007
  7. pmc Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli
    K C Murphy
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA
    J Bacteriol 180:2063-71. 1998
  8. pmc Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7
    Kenan C Murphy
    University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    J Bacteriol 190:438-41. 2008
  9. pmc Enhanced Actin Pedestal Formation by Enterohemorrhagic Escherichia coli O157:H7 Adapted to the Mammalian Host
    Michael John Brady
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School Worcester, MA, USA
    Front Microbiol 2:226. 2011
  10. ncbi request reprint Increased adherence and actin pedestal formation by dam-deficient enterohaemorrhagic Escherichia coli O157:H7
    Kenneth G Campellone
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Microbiol 63:1468-81. 2007

Research Grants

Collaborators

Detail Information

Publications14

  1. doi request reprint Rapid allelic exchange in enterohemorrhagic Escherichia coli (EHEC) and other E. coli using lambda red recombination
    Pamela J Savage
    University of Massachusetts MedicalSchool, Worcester, Massachusetts, USA
    Curr Protoc Microbiol . 2006
    ..coli K-12 and EHEC, this method has also been used for the construction of gene knockouts in enteropathogenic E. coli (EPEC), enteroaggregative E. coli, and uropathogenic E. coli, as well as Shigella flexneri and Salmonella enterica...
  2. doi request reprint Phage recombinases and their applications
    Kenan C Murphy
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA, USA
    Adv Virus Res 83:367-414. 2012
    ..Finally, the impact of this technology to such diverse fields as bacterial pathogenesis, metabolic engineering, and mouse genomics is discussed...
  3. ncbi request reprint PCR-mediated gene replacement in Escherichia coli
    K C Murphy
    Department of Molecular Genetics and Microbiology University of Massachusetts Medical School, Worcester, MA 01655, USA
    Gene 246:321-30. 2000
    ..coli. Three of these replacements were performed without prior cloning of the genes...
  4. doi request reprint Targeted chromosomal gene knockout using PCR fragments
    Kenan C Murphy
    Department of Microbial and Physiological systems, University of Massachusetts Medical School, Worcester, MA, USA
    Methods Mol Biol 765:27-42. 2011
    ..The basic protocols for designing PCR substrates for recombineering, generating -recombineering-proficient electrocompetent strains of E. coli, and for selection and verification of recombinant clones are described...
  5. pmc Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli
    Kenan C Murphy
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    BMC Mol Biol 4:11. 2003
    ..coli...
  6. ncbi request reprint The lambda Gam protein inhibits RecBCD binding to dsDNA ends
    Kenan C Murphy
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue, North, Worcester, MA 01655, USA
    J Mol Biol 371:19-24. 2007
    ..A revised model is proposed for Gam-induced radioresistance of E. coli to ionizing radiation...
  7. pmc Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli
    K C Murphy
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA
    J Bacteriol 180:2063-71. 1998
    ..The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable...
  8. pmc Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7
    Kenan C Murphy
    University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    J Bacteriol 190:438-41. 2008
    ..Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection...
  9. pmc Enhanced Actin Pedestal Formation by Enterohemorrhagic Escherichia coli O157:H7 Adapted to the Mammalian Host
    Michael John Brady
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School Worcester, MA, USA
    Front Microbiol 2:226. 2011
    ..These data suggest that adaptation of EHEC to the mammalian intestine enhances bacterial cell attachment, expression of intimin and Tir, and translocation of effectors that promote actin signaling...
  10. ncbi request reprint Increased adherence and actin pedestal formation by dam-deficient enterohaemorrhagic Escherichia coli O157:H7
    Kenneth G Campellone
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Microbiol 63:1468-81. 2007
    ..In contrast to other dam-deficient pathogens, EHECDeltadam is capable of robust intestinal colonization of experimentally infected animals...
  11. pmc High incidence of multiple antibiotic resistant cells in cultures of in enterohemorrhagic Escherichia coli O157:H7
    Benjamin R Carone
    Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, United States
    Mutat Res 759:1-8. 2014
    ..Spontaneous mutation frequency to rifampicin, spectinomycin and streptomycin resistance was the same in E. coli O157:H7 and E. coli K-12 strains. ..
  12. pmc Structural/functional analysis of the human OXR1 protein: identification of exon 8 as the anti-oxidant encoding function
    Kenan C Murphy
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655, USA
    BMC Mol Biol 13:26. 2012
    ..Multiple spliced isoforms are expressed in a variety of human tissues, including brain...
  13. pmc Roles of RuvC and RecG in phage lambda red-mediated recombination
    A R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 181:5402-8. 1999
    ..However, in all cases, the frequency of Red-mediated recombination was higher in recG than in recG+. These observations favor models in which RecG tends to push invading 3'-ended strands back out of recombination intermediates...
  14. ncbi request reprint Mutational analysis of the MutH protein from Escherichia coli
    T Loh
    Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 276:12113-9. 2001
    ..Two deletion mutations (MutHDelta224 and MutHDelta214) in the C-terminal end of the protein, localized the MutL stimulation region to five amino acids (Ala-220, Leu-221, Leu-222, Ala-223, and Arg-224)...

Research Grants1

  1. RECOMBINOGENIC ENGINEERING OF PATHOGENIC BACTERIA
    KENAN MURPHY; Fiscal Year: 2003
    ....