Research Topics
Genomes and GenesSpecies | Marcus MuenchSummaryAffiliation: Blood Systems Research Institute Location: San Francisco, UCA URL: http://web.mac.com/fogbank Summary: My research focus is on the development of the hematopoietic and hepatic systems. A driving force behind my basic research efforts is a desire for the research to be clinically applicable, particularly in the field of fetal medicine. Publications
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Publications
Coexpression of CD14 and CD326 discriminate hepatic precursors in the human fetal liverMarina E Fomin
Blood Systems Research Institute, San Francisco, California 94118, USA
Stem Cells Dev 20:1247-57. 2011....- Maternal microchimerism in the livers of patients with biliary atresiaDavid L Suskind
Department of Pediatrics, University of California, San Francisco, USA
BMC Gastroenterol 4:14. 2004..The aim of this study was to determine the presence and extent of maternal microchimerism (MM) in the livers of infants with BA... 
Haploidentical donor T cells fail to facilitate engraftment but lessen the immune response of host T cells in murine fetal transplantationJeng-Chang Chen
Fetal Treatment Center, Department of Surgery, University of California, San Francisco, CA 94143-0793, USA
Br J Haematol 126:377-84. 2004..The persistence of donor cells in the peritoneal cavity also correlated with tolerance induction...- Megakaryocyte growth and development factor is a potent growth factor for primitive hematopoietic progenitors in the human fetusMarcus O Muench
Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94143 0793, USA
Pediatr Res 55:1050-6. 2004.... - Stem cell transplantation in the fetusMarcus O Muench
Department of Laboratory Medicine, University of California, San Francisco, CA 94143, USA
Cancer Control 11:105-18. 2004..In utero transplantation (IUT) of hematopoietic stem cells has the potential to treat a large number of hematologic and metabolic diseases amenable to partial replacement of the hematopoietic system... - Searching for common stem cells of the hepatic and hematopoietic systems in the human fetal liver: CD34+ cytokeratin 7/8+ cells express markers for stellate cellsDavid L Suskind
Department of Pediatrics, University of California, San Francisco, CA 94143-0793, USA
J Hepatol 40:261-8. 2004..CONCLUSIONS: CD34+CK7/8+ cells are not common stem cells but rather appear to be hepatic stellate cells. A link between the hematopoietic and hepatic systems during fetal life requires further investigation... - A kinetic study of the murine mixed lymphocyte reaction by 5,6-carboxyfluorescein diacetate succinimidyl ester labelingJeng Chang Chen
Department of Surgery, University of California, San Francisco, CA, USA
J Immunol Methods 279:123-33. 2003..Among all the parameters, the relative number of daughter T-cells was the most practical for measuring MLR, after 5 days of culture, based upon the growth kinetics of responder T-cells and the survival of the stimulator cells... - Isolation of definitive zone and chromaffin cells based upon expression of CD56 (neural cell adhesion molecule) in the human fetal adrenal glandMarcus O Muench
Department of Laboratory Medicine and Center for Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USA
J Clin Endocrinol Metab 88:3921-30. 2003..These findings indicate that CD56 expression can be used to isolate DZ and chromaffin cells to further study their functional and developmental properties... - Ontogenic changes in CD95 expression on human leukocytes: prevalence of T-cells expressing activation markers and identification of CD95-CD45RO+ T-cells in the fetusMarcus O Muench
Department of Laboratory Medicine, University of California, 533 Parnassus Ave, 94143 0793, San Francisco, CA, USA
Dev Comp Immunol 27:899-914. 2003..Activation markers CD25, CD122, CD69 and CD80 were also present on fetal T-cells. These findings indicate that in the initial weeks following thymic maturation, a high frequency of T-cells is activated in the periphery of the fetus... - Broad distribution of colony-forming cells with erythroid, myeloid, dendritic cell, and NK cell potential among CD34(++) fetal liver cellsM O Muench
Department of Laboratory Medicine, University of California, San Francisco, CA 94143, USA
J Immunol 167:4902-9. 2001..Alternatively, some stem cells may be present among CD38(+) cells... - High-efficiency retroviral transduction of fetal liver CD38-CD34++ cells: implications for in utero and ex utero gene therapyT Ohkubo
Fetal Treatment Center, University of California at San Francisco, 94143-0793, USA
Fetal Diagn Ther 16:299-307. 2001..These results encourage further examination of in utero and ex utero gene therapy as a means of treating birth defects... - Hematopoietic stem cell transplantation in utero produces sheep-goat chimerasS M Oppenheim
Department of Animal Science, University of California, Davis, California 95616, USA
Blood Cells Mol Dis 27:296-308. 2001..The consistently low levels of chimerism observed in this study, as well as the poor results recently reported by others using these procedures, indicate that significant barriers exist to transplanting hematopoietic stem cells in utero... - Transplantation of a fetus with paternal Thy-1(+)CD34(+)cells for chronic granulomatous diseaseM O Muench
Fetal Treatment Center at the University of California, San Francisco 94143-0793, USA
Bone Marrow Transplant 27:355-64. 2001..The presence of these lymphocytes may contribute to the lack of donor cell engraftment in the human fetus... - Prevention of graft rejection by donor type II CD8(+) T cells (Tc2 cells) is not sufficient to improve engraftment in fetal transplantationJeng-Chang Chen
Fetal Treatment Center, Department of Surgery, University of California, San Francisco, CA 94143, USA
Fetal Diagn Ther 20:35-43. 2005..Donor cells can persist in the peritoneal cavity and might play an important role in inducing immune tolerance in fetuses... - In utero transplantation: baby steps towards an effective therapyM O Muench
Department of Laboratory Medicine, University of California, San Francisco, CA 94143 0793, USA
Bone Marrow Transplant 35:537-47. 2005..Continued research appears likely to succeed in developing IUT into an effective form of therapy for a variety of diseases... - Maintenance of proliferative capacity and retroviral transduction efficiency of human fetal CD38(-)/CD34(++) stem cellsMarcus O Muench
Department of Laboratory Medicine, University of California, San Francisco, CA 94143, USA
Stem Cells Dev 15:97-108. 2006..0%. These findings encourage further investigation into the feasibility of ex utero gene therapy whereby fetal cells are isolated from the circulation, transduced, and expanded ex utero before being returned to the fetus... 
Characterization of tolerance induction through prenatal marrow transplantation: the requirement for a threshold level of chimerism to establish rather than maintain postnatal skin toleranceJeng Chang Chen
Department of Surgery, Chang Gung Memorial Hospital, Chang Gung University, 5 Fu Shin Street, Kweishan, Taoyuan, Taiwan
Cell Transplant 19:1609-22. 2010..Therefore, the diminution of hematopoietic chimerism below a threshold level does not nullify an existing tolerance state, but lessens the chance of enabling complete tolerance...- Requirement of retinoids for the expression of CD38 on human hematopoietic progenitors in vitroM O Muench
Fetal Treatment Center Research Laboratory, University of California at San Francisco, 513 Parnassus Avenue Room HSW 1601, San Francisco, CA 94143 0570, USA
Cytotherapy 1:455-68. 1999..In this study we sought to determine the feasibility of using CD34 and CD38 as markers of hematopoietic differentiation in vitro, using retinoids to induce the expression of CD38... - Differential downregulation of ACE2 by the spike proteins of severe acute respiratory syndrome coronavirus and human coronavirus NL63Ilona Glowacka
Institute of Virology, OE 5230, Hannover Medical School, Carl Neuberg Str 1, 30625 Hannover, Germany
J Virol 84:1198-205. 2010..Finally, SARS-CoV but not NL63 replicated efficiently in ACE2-positive Vero cells and reduced ACE2 expression, indicating robust receptor interference in the context of SARS-CoV but not NL63 infection... - Barley as a green factory for the production of functional Flt3 ligandLýdur S Erlendsson
ORF Genetics, Keldnaholt, Reykjavik, Iceland
Biotechnol J 5:163-71. 2010..The present results demonstrate that plant molecular farming is a viable approach for the bioproduction of human-derived growth factors... - Characterization of Chikungunya pseudotyped viruses: Identification of refractory cell lines and demonstration of cellular tropism differences mediated by mutations in E1 glycoproteinBeatriz Salvador
Blood Systems Research Institute, San Francisco, CA 94118, USA
Virology 393:33-41. 2009..CHIKV pseudotypes will serve as an important tool for the study of neutralizing antibodies and the analysis of envelope glycoprotein functions... - A new role for the human placenta as a hematopoietic site throughout gestationAlicia Barcena
Institute for Regeneration Medicine, Human Embryonic Stem Cell Program, Department of Cell and Tissue Biology, University of California at San Francisco, California 94143, USA
Reprod Sci 16:178-87. 2009..Together, these data suggest that the human placenta is potentially an important hematopoietic organ, opening the possibility of banking placental hematopoietic stem cells along with cord blood for transplantation... - The human placenta is a hematopoietic organ during the embryonic and fetal periods of developmentAlicia Barcena
Institute for Regeneration Medicine, Human Embryonic Stem Cell Program, Department of Cell and Tissue Biology, University of California, San Francisco, 513 Parnassus Avenue, HSE 1619, San Francisco, California 94143 0512, USA
Dev Biol 327:24-33. 2009..These data suggest that the human placenta is an important hematopoietic organ, raising the possibility of banking placental hematopoietic stem cells along with cord blood for transplantation... - Transplantation NK'Oed in the first trimesterMarcus O Muench
Blood Systems Research Institute
Blood 112:4790-1. 2008 - Maternal alloantigens promote the development of tolerogenic fetal regulatory T cells in uteroJeff E Mold
Division of Experimental Medicine, Department of Medicine, University of California at San Francisco UCSF, San Francisco, CA 94110, USA
Science 322:1562-5. 2008..These findings reveal a form of antigen-specific tolerance in humans, induced in utero and probably active in regulating immune responses after birth... - Polysialic acid, a glycan with highly restricted expression, is found on human and murine leukocytes and modulates immune responsesPenelope M Drake
Department of Chemistry, University of California, Berkeley, CA 94720, USA
J Immunol 181:6850-8. 2008..This is the first demonstration of polySia expression and regulation on myeloid cells, and the results in animal models suggest a role for polySia in immune regulation... - Prenatal tolerance induction: relationship between cell dose, marrow T-cells, chimerism, and toleranceJeng Chang Chen
Department of Surgery, Chang Gung Children s Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan
Cell Transplant 17:495-506. 2008..Thus, peripheral chimerism level can be a simple and straightforward test to predict the degree of prenatal immune tolerance... - Persistence of allografts in the peritoneal cavity after prenatal transplantation in miceJeng Chang Chen
Department of Surgery, University of California, San Francisco, California, USA
Transfusion 48:553-60. 2008..In utero transplantation (IUT) is usually performed by intraperitoneal injection, but there is little information regarding the fate of intraperitoneally transplanted cells in the first weeks after transplantation... - Midkine, a heparin-binding growth factor, selectively stimulates proliferation of definitive zone cells of the human fetal adrenal glandHitoshi Ishimoto
Center for Reproductive Sciences, 1450 Health Sciences West, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143 0556, USA
J Clin Endocrinol Metab 91:4050-6. 2006..Midkine (MK) is a heparin-binding growth factor with various bioactivities... - Disparate regulation of human fetal erythropoiesis by the microenvironments of the liver and bone marrowM O Muench
University of California, San Francisco, California 94143, USA
Blood Cells Mol Dis 27:377-90. 2001..These data implicate a critical role for the microenvironments of the liver and BM in regulating the disparate levels of erythropoiesis in these tissues... - Antibodies to human fetal erythroid cells from a nonimmune phage antibody libraryM A Huie
Department of Dermatology, University of California, San Francisco, CA 94143, USA
Proc Natl Acad Sci U S A 98:2682-7. 2001..Some of the antibodies may also have possible therapeutic utility for erythroleukemia... - FLK-2/FLT-3 ligand regulates the growth of early myeloid progenitors isolated from human fetal liverM O Muench
Human Immunology and Molecular Biology Departments, DNAX Research Institute, Palo Alto, CA
Blood 85:963-72. 1995..These results indicate that FL is part of a network of growth factors that regulate the growth and survival of early hematopoietic progenitors... - In search of T-cell progenitors in the human foetal liverA Barcena
DNAX Research Institute, Human Immunology Department, Palo Alto, CA
Res Immunol 145:120-3; discussion 155-8. 1994 - Identification of a common T/natural killer cell progenitor in human fetal thymusM J Sanchez
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304
J Exp Med 180:569-76. 1994..These results represent the first definitive evidence of a common T/NK cell progenitor in the human fetal thymus and delineate the point in thymocyte differentiation where T and NK cells diverge... - Lymphoid and myeloid differentiation of fetal liver CD34+lineage- cells in human thymic organ cultureA Barcena
Human Immunology Department, DNAX Research Institute, Palo Alto, California 94304
J Exp Med 180:123-32. 1994..These results demonstrate that the microinjection of fetal liver hematopoietic progenitors into fetal thymic organ fragments results in multilineage differentiation in vitro... - Expression of CD33, CD38, and HLA-DR on CD34+ human fetal liver progenitors with a high proliferative potentialM O Muench
Human Immunology Department, DNAX Research Institute, Palo Alto, CA
Blood 83:3170-81. 1994..These data suggest that FL HPP-CFC represent a heterogeneous compartment of primitive myeloid progenitors that may include stem cells... - Radioprotection by murine and human tumor-necrosis factor: dose-dependent effects on hematopoiesis in the mouseL Slørdal
James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, New York, NY
Eur J Haematol 43:428-34. 1989..This suggests that TNF exerts it radioprotective effects on a pool of primitive multi-potential hematopoietic cells... - Cytokine networks involved in the regulation of haemopoietic stem cell proliferation and differentiationM A Moore
James Ewing Laboratory of Developmental Hematopoiesis, Memoral Sloan Kettering Cancer Center, New York, NY 10021
Ciba Found Symp 148:43-58; discussion 58-61. 1990..Induction of products of the cyclooxygenase and lipooxygenase pathways, as well as tumour necrosis factor and TGF-beta, modulate haemopoiesis... - Purification and partial characterization of a human hematopoietic precursor populationC Smith
Bone Marrow Transplant Service, Memorial Sloan Kettering Cancer Center, New York, NY 10021
Blood 77:2122-8. 1991.... - Accelerated recovery of peripheral blood cell counts in mice transplanted with in vitro cytokine-expanded hematopoietic progenitorsM O Muench
James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, New York, New York 10021
Exp Hematol 20:611-8. 1992..The expansion of BM progenitor cells without loss of long-term proliferative potential may be of clinical importance in the fields of BM transplantation and gene therapy... - Ex vivo differentiation therapy as a method of leukemic cell purging in murine bone marrow expansion culturesM O Muench
James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, New York, New York 10021
Cancer Res 52:6576-82. 1992..The addition of G-CSF, mutein, and ATRA to delta-cultures did not result in any impairment of hematopoietic stem cells when measured 5 wk after transplantation... - Interactions among colony-stimulating factors, IL-1 beta, IL-6, and kit-ligand in the regulation of primitive murine hematopoietic cellsM O Muench
James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, New York, New York
Exp Hematol 20:339-49. 1992..The ability to expand early progenitor cells in delta-cultures was further demonstrated by the greater than 100-fold expansions of day-12 spleen colony-forming units (CFU-S) by the synergistic interactions of IL-1 with IL-3 or KL... - The in vitro growth of murine high proliferative potential-colony forming cells is not enhanced by growth in a low oxygen atmosphereM O Muench
James Ewing Laboratory of Developmental Hematopoiesis, Memorial Sloan Kettering Cancer Center, New York, NY 10021
Cytokine 4:488-94. 1992..These results demonstrate that the growth of murine HPP-CFC was not compromised when cultured at ambient O2 concentration... - [Birth-conditioned cerebral vascular disorders in children]A I Kaĭsarova
Vopr Okhr Materin Det 23:49-52. 1978 - Tracing the expression of CD7 and other antigens during T- and myeloid-cell differentiation in the human fetal liver and thymusA Barcena
Human Immunology Department, DNAX Research Institute for Cellular and Molecular Biology, Palo Alto, California 94304, USA
Leuk Lymphoma 17:1-11. 1995..In addition, we present an hypothetical model of hematopoiesis in the fetal liver and thymus... - Phenotypic and functional analysis of T-cell precursors in the human fetal liver and thymus: CD7 expression in the early stages of T- and myeloid-cell developmentA Barcena
Human Immunology Department, DNAX Research Institute, Palo Alto, CA 94304 1104
Blood 82:3401-14. 1993.... - Progress in the ex vivo expansion of hematopoietic progenitorsM O Muench
Human Immunology Department, DNAX Research Institute, Palo Alto, CA 94304 1104
Leuk Lymphoma 16:1-11. 1994..Thus, ex vivo progenitor expansion may soon be of use in transplantation protocols to accelerate hematopoietic reconstitution and in gene therapy protocols if hematopoietic stem cells can be maintained during ex vivo culture... - Differential effects of interleukin-3, interleukin-7, interleukin 15, and granulocyte-macrophage colony-stimulating factor in the generation of natural killer and B cells from primitive human fetal liver progenitorsM O Muench
The Fetal Treatment Center Research Laboratory, UCSF Cancer Research Center, University of California, San Francisco, CA, USA
Exp Hematol 28:961-73. 2000..These findings indicate a differential regulation of NK and B lymphopoiesis beginning in the early stages of hematopoiesis as exemplified by the distinctive roles of IL-7, IL-15, GM-CSF, and IL-3... - Fetal bone marrow as a source of stem cells for in utero or postnatal transplantationF Golfier
Department of Surgery, Fetal Treatment Center Research Laboratory at the University of California, San Francisco, CA 94143 0570, USA
Br J Haematol 109:173-81. 2000..Thus, FBM is a safe source of stem cells. The large number of progenitors and candidate stem cells that can be obtained from FBM makes it suitable for in utero and possibly postnatal transplantation... - Mid-trimester fetal livers are a rich source of CD34+/++ cells for transplantationF Golfier
Department of Surgery, Fetal Treatment Center Research Laboratory, University of California, San Francisco, CA 94143 0570, USA
Bone Marrow Transplant 24:451-61. 1999..In conclusion, purification of FL progenitors between 16 and 24 weeks of gestation results in a large number of early progenitors suitable for in utero and possibly post-natal transplantation... - Role of CD95/Fas and its ligand in the regulation of the growth of human CD34(++)CD38(-) fetal liver cellsA Barcena
The Fetal Treatment Center Research Laboratory, University of California, San Francisco 94143 0570, USA
Exp Hematol 27:1428-39. 1999..We conclude that the endogenous CD95L has an inhibitory effect on fetal candidate HSCs, which can be blocked by sCD95L and CH-11 mAb... - Administration of Flk2/Flt3 ligand induces expansion of human high-proliferative potential colony-forming cells in the SCID-hu mouseR Namikawa
DNAX Research Institute of Molecular and Cellular Biology, Inc, Palo Alto, CA, USA
Exp Hematol 27:1029-37. 1999..These results indicate that FL induces the expansion of primitive hematopoietic progenitor cells in vivo and, therefore, may be useful in treating patients to promote an early hematopoietic recovery after cytoablative therapies... - The biology and ethics of banking fetal liver hematopoietic stem cells for in utero transplantationG B Mychaliska
Department of Surgery, The Fetal Treatment Center, University of California, San Francisco 94143 0570, USA
J Pediatr Surg 33:394-9. 1998..The authors describe their methods for processing fetal liver free of known human pathogens while maximizing HSC activity after cryopreservation... - Colony-forming cells expressing high levels of CD34 are the main targets for granulocyte colony-stimulating factor and macrophage colony-stimulating factor in the human fetal liverM O Muench
Human Immunology Department, DNAX Research Institute, Palo Alto, CA, USA
Exp Hematol 25:277-87. 1997..These data help to define the potential roles of cytokines in human fetal hematopoiesis... - Phenotypic and functional evidence for the expression of CD4 by hematopoietic stem cells isolated from human fetal liverM O Muench
Human Immunology Department, DNAX Research Institute, Palo Alto, CA 94304 1104, USA
Blood 89:1364-75. 1997..Furthermore, all CD4+ CD34++ Lin- fetal liver cells also expressed CD13 and CD33... - Regulatory roles of the ligand for Flk2/Flt3 tyrosine kinase receptor on human hematopoiesisR Namikawa
DNAX Research Institute of Molecular and Cellular Biology, Inc, Palo Alto, California 94304, USA
Stem Cells 14:388-95. 1996..Thus, FL plays important roles in regulating the proliferation of hematopoietic progenitor cells and, therefore, may have therapeutic applications... - The FLK2/FLT3 ligand synergizes with interleukin-7 in promoting stromal-cell-independent expansion and differentiation of human fetal pro-B cells in vitroR Namikawa
Human Immunology Department, DNAX Research Institute of Molecular and Cellular Biology, Inc, Palo Alto, CA, USA
Blood 87:1881-90. 1996.... - Development of a lacZ marked WEHI-3B D+ murine leukemic cell line as an in-vivo model of acute non-lymphocytic leukemiaC Smith
Division of Hematologic Oncology, Memorial Sloan Kettering Cancer Center, New York, NY
Leukemia 7:310-7. 1993..Results indicate that treatment of mice inoculated with WEHI-3B D+/lacZ 2.8 cells with G-CSF administration causes detectable but limited differentiation... - Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAsC Hannum
DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304 1104
Nature 368:643-8. 1994.... - Interleukin-11 expression in donor bone marrow cells improves hematological reconstitution in lethally irradiated recipient miceS R Paul
Genetics Institute, Cambridge, MA 02140
Exp Hematol 22:295-301. 1994..No abnormal cell proliferation or other histopathology was observed in the hIL-11-expressing mice...