Iwona Mruk

Summary

Affiliation: University of Toledo
Country: USA

Publications

  1. ncbi Characterization of the LlaCI methyltransferase from Lactococcus lactis subsp. cremoris W15 provides new insights into the biology of type II restriction-modification systems
    Iwona Mruk
    Department of Microbiology, , , , Poland
    Microbiology 149:3331-41. 2003
  2. ncbi Real-time kinetics of restriction-modification gene expression after entry into a new host cell
    Iwona Mruk
    Department of Medical Microbiology and Immunology, University of Toledo Health Sciences Campus, Toledo, OH 43614 2598, USA
    Nucleic Acids Res 36:2581-93. 2008
  3. ncbi Tuning the relative affinities for activating and repressing operators of a temporally regulated restriction-modification system
    Iwona Mruk
    Department of Medical Microbiology and Immunology, University of Toledo Health Sciences Campus, Toledo, OH 43614 2598, USA
    Nucleic Acids Res 37:983-98. 2009
  4. ncbi Genetic organization and molecular analysis of the EcoVIII restriction-modification system of Escherichia coli E1585-68 and its comparison with isospecific homologs
    Iwona Mruk
    Department of Microbiology, University of Gdansk, Kladki 24, 80 822 Gdansk, Poland
    Appl Environ Microbiol 69:2638-50. 2003

Collaborators

Detail Information

Publications4

  1. ncbi Characterization of the LlaCI methyltransferase from Lactococcus lactis subsp. cremoris W15 provides new insights into the biology of type II restriction-modification systems
    Iwona Mruk
    Department of Microbiology, , , , Poland
    Microbiology 149:3331-41. 2003
    ..This observation suggests that sensitivity of the M.LlaCI to Mg(2+) may strengthen the restriction activity of the cognate endonuclease in the bacterial cell. Other biological implications of this finding are also discussed...
  2. ncbi Real-time kinetics of restriction-modification gene expression after entry into a new host cell
    Iwona Mruk
    Department of Medical Microbiology and Immunology, University of Toledo Health Sciences Campus, Toledo, OH 43614 2598, USA
    Nucleic Acids Res 36:2581-93. 2008
    ..Surprisingly, though the C and REase genes are cotranscribed, the pvuIIC portion of the mRNA was more abundant than the pvuIIR portion after stable establishment of the R-M system...
  3. ncbi Tuning the relative affinities for activating and repressing operators of a temporally regulated restriction-modification system
    Iwona Mruk
    Department of Medical Microbiology and Immunology, University of Toledo Health Sciences Campus, Toledo, OH 43614 2598, USA
    Nucleic Acids Res 37:983-98. 2009
    ..The results suggest that the GACTnnnAGTC consensus is the primary determinant of C.PvuII binding affinity, with intra-operator spacers playing a fine-tuning role that affects mobility of this R-M system...
  4. ncbi Genetic organization and molecular analysis of the EcoVIII restriction-modification system of Escherichia coli E1585-68 and its comparison with isospecific homologs
    Iwona Mruk
    Department of Microbiology, University of Gdansk, Kladki 24, 80 822 Gdansk, Poland
    Appl Environ Microbiol 69:2638-50. 2003
    ..LlaCI but not with M. HindIII. R. EcoVIII requires Mg(II) ions for phosphodiester bond cleavage. We found that the same ions are strong inhibitors of the M. EcoVIII enzyme. The biological implications of this finding are discussed...