D O Morgan

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Regulation of the APC and the exit from mitosis
    D O Morgan
    Department of Physiology, University of California, San Francisco 94143 0444, USA
    Nat Cell Biol 1:E47-53. 1999
  2. pmc Cak1 is required for Kin28 phosphorylation and activation in vivo
    F H Espinoza
    Departments of Physiology and Biochemistry and Biophysics, University of California, San Francisco, California 94143 0444, USA
    Mol Cell Biol 18:6365-73. 1998
  3. pmc A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae
    S L Jaspersen
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    Mol Biol Cell 9:2803-17. 1998
  4. ncbi request reprint Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14
    S L Jaspersen
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    Curr Biol 9:227-36. 1999
  5. ncbi request reprint Cdc14 activates cdc15 to promote mitotic exit in budding yeast
    S L Jaspersen
    Department of Physiology, University of California, San Francisco, CA 94143 0444, USA
    Curr Biol 10:615-8. 2000
  6. ncbi request reprint A cyclin-dependent kinase-activating kinase (CAK) in budding yeast unrelated to vertebrate CAK
    F H Espinoza
    Department of Physiology, University of California, San Francisco, 94143 0444, USA
    Science 273:1714-7. 1996
  7. pmc Cdc37 promotes the stability of protein kinases Cdc28 and Cak1
    A Farrell
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    Mol Cell Biol 20:749-54. 2000
  8. ncbi request reprint Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors
    N S Gray
    Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Science 281:533-8. 1998
  9. ncbi request reprint A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase
    R P Fisher
    Department of Physiology, University of California, San Francisco 94143 0444
    Cell 78:713-24. 1994
  10. pmc Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta
    C G Takizawa
    Department of Physiology, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 96:7938-43. 1999

Collaborators

  • J F Charles
  • Kim Sung-Hoon
  • J Rosenblatt
  • R J Deshaies
  • D J Lockhart
  • G Barnes
  • K Weis
  • K K Kim
  • T C Norman
  • Y Gu
  • L Meijer
  • Dun Yang
  • Andrei Goga
  • J Ogas
  • Liam J Holt
  • Justin D Blethrow
  • S L Jaspersen
  • Erika L Woodbury
  • F H Espinoza
  • Kevan M Shokat
  • A Farrell
  • C G Takizawa
  • Maria Enquist-Newman
  • Nolan Ko
  • Jeffrey A Ubersax
  • Brian C Kraybill
  • R P Fisher
  • P Jin
  • Matt Sullivan
  • Joseph S Glavy
  • Andrew N Krutchinsky
  • Jessica E Hutti
  • Ryuichi Nishihama
  • Lewis C Cantley
  • John R Pringle
  • Gregory H Tully
  • Denis Ostapenko
  • Mark J Solomon
  • N S Gray
  • Maria Paraz
  • H M Chamberlin
  • Phuong N Quang
  • Kavita Shah
  • Lisa L Elkin
  • D Desai
  • M R Gerber
  • I Herskowitz
  • L Wodicka
  • A M Thunnissen
  • P G Schultz
  • S H Kim
  • S Kwon
  • O Gileadi
  • R L Tinker-Kulberg
  • S Leclerc
  • J L Nourse
  • S Hardy
  • P Tempst
  • H Erdjument-Bromage
  • H C Wessling

Detail Information

Publications31

  1. ncbi request reprint Regulation of the APC and the exit from mitosis
    D O Morgan
    Department of Physiology, University of California, San Francisco 94143 0444, USA
    Nat Cell Biol 1:E47-53. 1999
    ..An intricate regulatory network governs APC activity and helps to ensure that late mitotic events are properly timed and coordinated...
  2. pmc Cak1 is required for Kin28 phosphorylation and activation in vivo
    F H Espinoza
    Departments of Physiology and Biochemistry and Biophysics, University of California, San Francisco, California 94143 0444, USA
    Mol Cell Biol 18:6365-73. 1998
    ..We conclude that Cak1 is required for the activating phosphorylation of Kin28 as well as that of Cdc28...
  3. pmc A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae
    S L Jaspersen
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    Mol Biol Cell 9:2803-17. 1998
    ..Mutant cells arrested in G1 contain normal APC activity. We conclude that Cdc15, Cdc5, Cdc14, Dbf2, and Tem1 cooperate in the activation of the APC in late mitosis but are not required for maintenance of that activity in G1...
  4. ncbi request reprint Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14
    S L Jaspersen
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    Curr Biol 9:227-36. 1999
    ..Here, we explore the molecular function and regulation of the APC regulatory subunit Hct1 in Saccharomyces cerevisiae...
  5. ncbi request reprint Cdc14 activates cdc15 to promote mitotic exit in budding yeast
    S L Jaspersen
    Department of Physiology, University of California, San Francisco, CA 94143 0444, USA
    Curr Biol 10:615-8. 2000
    ..Instead, Cdc15 dephosphorylation may promote some additional function of Cdc15 that is independent of its effects on Cdc14 activation...
  6. ncbi request reprint A cyclin-dependent kinase-activating kinase (CAK) in budding yeast unrelated to vertebrate CAK
    F H Espinoza
    Department of Physiology, University of California, San Francisco, 94143 0444, USA
    Science 273:1714-7. 1996
    ..The CAK1 gene was essential for cell viability. Thus, the major CAK in S. cerevisiae is distinct from the vertebrate enzyme, suggesting that budding yeast and vertebrates may have evolved different mechanisms of CDK activation...
  7. pmc Cdc37 promotes the stability of protein kinases Cdc28 and Cak1
    A Farrell
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    Mol Cell Biol 20:749-54. 2000
    ..We conclude that budding yeast Cdc37, like its higher eukaryotic homologs, promotes the physical integrity of multiple protein kinases, perhaps by virtue of a cotranslational role in protein folding...
  8. ncbi request reprint Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors
    N S Gray
    Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Science 281:533-8. 1998
    ..Purine libraries could provide useful tools for analyzing a variety of signaling and regulatory pathways and may lead to the development of new therapeutics...
  9. ncbi request reprint A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase
    R P Fisher
    Department of Physiology, University of California, San Francisco 94143 0444
    Cell 78:713-24. 1994
    ..Thus, CAK is a CDK-cyclin complex implicated in the control of multiple cell cycle transitions...
  10. pmc Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta
    C G Takizawa
    Department of Physiology, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 96:7938-43. 1999
    ..We conclude that cyclin B1 import is mediated by an unusual importin beta-dependent mechanism that does not require Ran...
  11. pmc Nuclear localization of cyclin B1 controls mitotic entry after DNA damage
    P Jin
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    J Cell Biol 141:875-85. 1998
    ..Thus, nuclear targeting of cyclin B1 and dephosphorylation of Cdc2 both contribute to the control of mitotic entry and exit in human cells...
  12. pmc Effects of phosphorylation by CAK on cyclin binding by CDC2 and CDK2
    D Desai
    Department of Physiology, University of California, San Francisco 94143 0444
    Mol Cell Biol 15:345-50. 1995
    ..CDC2 does not bind with high affinity to cyclin E in vitro, even after phosphorylation of the CDC2 subunit. Thus, phosphorylation is of varying importance in the formation of high-affinity CDK-cyclin complexes...
  13. ncbi request reprint Cell cycle control by a complex of the cyclin HCS26 (PCL1) and the kinase PHO85
    F H Espinoza
    Department of Physiology, University of California, San Francisco 94143
    Science 266:1388-91. 1994
    ..HCS26 does not associate with CDC28, but instead associates with PHO85, a closely related protein kinase. Thus, budding yeast, like higher eukaryotes, use multiple cdk's in the regulation of cell cycle progression...
  14. ncbi request reprint The Polo-related kinase Cdc5 activates and is destroyed by the mitotic cyclin destruction machinery in S. cerevisiae
    J F Charles
    Department of Physiology, University of California San Francisco, California, 94143, USA
    Curr Biol 8:497-507. 1998
    ..The Polo-related protein kinase Cdc5 in Saccharomyces cerevisiae might encode a regulator of the APC, because cdc5 mutant cells arrest with a late mitotic phenotype similar to that observed in cells with defective cyclin destruction...
  15. ncbi request reprint Three-dimensional structure of human cyclin H, a positive regulator of the CDK-activating kinase
    K K Kim
    Department of Chemistry, University of California, Berkeley 94720, USA
    Nat Struct Biol 3:849-55. 1996
    ..Outside of the core domains, the N- and C-terminal regions of the three structures are completely different. The conformational differences between cyclin H and A structures may reflect functional differences between the two cyclins...
  16. ncbi request reprint Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C
    C G Takizawa
    Department of Physiology, University of California, San Francisco, CA 94143 0444, USA
    Curr Opin Cell Biol 12:658-65. 2000
    ..Phosphorylation-dependent changes in the rate of nuclear import and export of these proteins help to control the onset of mitosis both in normal cells and in cells delayed before mitosis by DNA damage...
  17. pmc Cdc37 is required for association of the protein kinase Cdc28 with G1 and mitotic cyclins
    M R Gerber
    Department of Physiology, University of California, San Francisco 94143 0444, USA
    Proc Natl Acad Sci U S A 92:4651-5. 1995
    ..cdc37-1 mutants also exhibit a defect in the binding and activation of Cdc28 by the mitotic cyclin Clb2. Thus Cdc37 may be a regulator that is required for the association of Cdc28 with multiple cyclins...
  18. ncbi request reprint The dynamics of cyclin dependent kinase structure
    D O Morgan
    Department of Physiology, Box 0444, University of California, 513 Parnassus Ave, San Francisco, CA 94143, USA
    Curr Opin Cell Biol 8:767-72. 1996
    ....
  19. ncbi request reprint Cyclin-dependent kinases: engines, clocks, and microprocessors
    D O Morgan
    Department of Physiology, University of California, San Francisco 94143 0444, USA
    Annu Rev Cell Dev Biol 13:261-91. 1997
    ..In the cell, these regulatory mechanisms generate an interlinked series of Cdk oscillators that trigger the events of cell division...
  20. ncbi request reprint Principles of CDK regulation
    D O Morgan
    Department of Physiology, University of California, San Francisco 94143 0444
    Nature 374:131-4. 1995
    ..The activity of cyclin-dependent kinases is controlled by four highly conserved biochemical mechanisms, forming a web of regulatory pathways unmatched in its elegance and intricacy...
  21. ncbi request reprint Alternative mechanisms of CAK assembly require an assembly factor or an activating kinase
    R P Fisher
    Department of Physiology, University of California, San Francisco 94143 0444, USA
    Cell 83:47-57. 1995
    ..Thus, CDK7-cyclin H complex formation can be achieved by multiple mechanisms...
  22. pmc Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15
    Y Gu
    Department of Physiology, University of California, San Francisco 94143 0444
    EMBO J 11:3995-4005. 1992
    ..Phosphorylation on the inhibitory sites T14 and Y15 is also maximal during S phase and G2. Thus, the activity of a subpopulation of CDK2 molecules is inhibited at a time in the cell cycle when overall CDK2 activity is increased...
  23. ncbi request reprint Inhibition of CDK1 as a potential therapy for tumors over-expressing MYC
    Andrei Goga
    Department of Medicine, Division of Hematology Oncology, University of California, San Francisco, San Francisco, California 94143 0552, USA
    Nat Med 13:820-7. 2007
    ..As there are no effective small-molecule inhibitors that selectively target the MYC pathway, we propose that CDK1 inhibition might therefore be useful in the treatment of human malignancies that overexpress MYC...
  24. ncbi request reprint Targets of the cyclin-dependent kinase Cdk1
    Jeffrey A Ubersax
    Department of Physiology, University of California, San Francisco, California 94143, USA
    Nature 425:859-64. 2003
    ..Detailed analysis of these substrates is likely to yield important insights into cell-cycle regulation...
  25. ncbi request reprint Cdk and APC activities limit the spindle-stabilizing function of Fin1 to anaphase
    Erika L Woodbury
    Department of Physiology, University of California, UCSF Mailcode 2200, Genentech Hall Room N312B, 600 16th Street, San Francisco, CA 94158 2517, USA
    Nat Cell Biol 9:106-12. 2007
    ..Our studies illustrate how phosphorylation-dependent changes in the behaviour of Cdk1 substrates influence complex mitotic processes...
  26. pmc Evolution of Ime2 phosphorylation sites on Cdk1 substrates provides a mechanism to limit the effects of the phosphatase Cdc14 in meiosis
    Liam J Holt
    Department of Physiology, University of California San Francisco, San Francisco, CA 94158, USA
    Mol Cell 25:689-702. 2007
    ..We propose that Ime2-dependent phosphorylation of a subset of cell-cycle proteins limits the effects of Cdc14 in meiosis...
  27. ncbi request reprint Inhibitor scaffolds as new allele specific kinase substrates
    Brian C Kraybill
    Department of Cellular and Molecular Pharmacology, Box 0450, University of California San Francisco, San Francisco, California 94143, USA
    J Am Chem Soc 124:12118-28. 2002
    ..The fact that this new more highly orthogonal nucleotide is accepted by three widely divergent kinases studied here suggests that it is likely to be generalizable across the entire kinase superfamily...
  28. pmc Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis
    Nolan Ko
    Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 18:5139-53. 2007
    ..Together, the data suggest that compromise of APC/C function allows the accumulation of Iqg1p, which then promotes actomyosin-ring-independent cytokinesis at least in part by activation of Cyk3p...
  29. pmc Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates
    Justin D Blethrow
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 105:1442-7. 2008
    ..This approach has the potential to expand our understanding of kinase-substrate connections in signaling networks...
  30. pmc Modulation of the mitotic regulatory network by APC-dependent destruction of the Cdh1 inhibitor Acm1
    Maria Enquist-Newman
    Department of Physiology, University of California, San Francisco, San Francisco, CA 94158 2517, USA
    Mol Cell 30:437-46. 2008
    ..We also speculate that the ability of APC(Cdh1) to target its own inhibitor enhances the bistability of the late mitotic regulatory system...
  31. pmc Positive feedback sharpens the anaphase switch
    Liam J Holt
    Department of Physiology, University of California, San Francisco, California 94158, USA
    Nature 454:353-7. 2008
    ..Our results also suggest that coupling securin degradation with changes in Cdk1 and Cdc14 activities helps coordinate the initiation of sister-chromatid separation with changes in spindle dynamics...