Research Topics
Species | John MoranSummaryAffiliation: University of Michigan Country: USA Publications
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Publications
Cis-preferential LINE-1 reverse transcriptase activity in ribonucleoprotein particlesDeanna A Kulpa
Department of Human Genetics 1241 E Catherine St, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
Nat Struct Mol Biol 13:655-60. 2006..We further demonstrate that ORF2p preferentially uses its encoding RNA as a template for reverse transcription. Thus, our data provide the first biochemical evidence supporting the cis-preferential action of the L1 reverse transcriptase...- Exon shuffling by L1 retrotranspositionJ V Moran
Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104 6145 USA
Science 283:1530-4. 1999..Thus, retrotransposition-competent L1s provide a vehicle to mobilize non-L1 sequences, such as exons or promoters, into existing genes and may represent a general mechanism for the evolution of new genes... - Human L1 retrotransposition: insights and peculiarities learned from a cultured cell retrotransposition assayJ V Moran
Department of Human Genetics and Internal Medicine, The University of Michigan Medical School, Ann Arbor 48109 0618, USA
Genetica 107:39-51. 1999..In general, I will limit the discussion to studies conducted on human L1s. However, interesting parallels to rodent L1s and other non-LTR retrotransposons also will be discussed... 
Allelic heterogeneity in LINE-1 retrotransposition activitySheila M Lutz
Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109 0618, USA
Am J Hum Genet 73:1431-7. 2003..2A retrotransposition efficiency. Thus, common L1 alleles can vary widely in their retrotransposition potential. We propose that such allelic heterogeneity can influence the potential L1 mutational load present in an individual genome...- LINE-1 retrotransposition activity in human genomesChristine R Beck
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Cell 141:1159-70. 2010..Therefore, these results suggest that hot L1s are more abundant in the human population than previously appreciated, and that ongoing L1 retrotransposition continues to be a major source of interindividual genetic variation... - LINE-1 retrotransposition in human embryonic stem cellsJose L Garcia Perez
Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, 1241 E Catherine Street, Ann Arbor, MI 48109, USA
Hum Mol Genet 16:1569-77. 2007..Thus, these data suggest that LINE-1 retrotransposition events may occur during early stages of human development... - Selective inhibition of Alu retrotransposition by APOBEC3GAmy E Hulme
Department of Human Genetics, The University of Michigan Medical School, Ann Arbor, MI 48109, USA
Gene 390:199-205. 2007..These data demonstrate a differential restriction of L1 and Alu retrotransposition by APOBEC3G, and suggest that the Alu ribonucleoprotein complex may be targeted by APOBEC3G... - Multiple forms of genetic instability within a 2-Mb chromosomal segment of 3q26.3-q27 are associated with development of esophageal adenocarcinomaLin Lin
Department of Surgery Thoracic Section, University of Michigan Medical School, B560 MSRB2, Box 0686, Ann Arbor, MI 48109, USA
Genes Chromosomes Cancer 45:319-31. 2006..Thus, the fine dissection of a 2-Mb amplified DNA segment in 3q26.3-q27 in EA revealed multiple genetic alterations that had occurred sequentially and/or concurrently during EA development... - Epigenetic silencing of engineered L1 retrotransposition events in human embryonic carcinoma cellsJose L Garcia-Perez
Department of Human Genetics, 1241 East Catherine Street, University of Michigan Medical School, Ann Arbor, Michigan 48109 5618, USA
Nature 466:769-73. 2010..Thus, our data indicate that ECs differ from many differentiated cells in their ability to silence reporter genes delivered by L1 retrotransposition... - Genomic deletions created upon LINE-1 retrotranspositionNicolas Gilbert
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Cell 110:315-25. 2002..Thus, our data demonstrate multiple pathways for L1 integration in cultured cells, and show that L1 is not simply an insertional mutagen, but that its retrotransposition can result in significant deletions of genomic sequence... - ATLAS: a system to selectively identify human-specific L1 insertionsRichard M Badge
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Am J Hum Genet 72:823-38. 2003..Thus, ATLAS provides a simple, high-throughput means to assess genetic variation associated with L1 retrotransposons... - Hot L1s account for the bulk of retrotransposition in the human populationBrook Brouha
Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 100:5280-5. 2003..Thus, our data indicate that most L1 retrotransposition in the human population stems from hot L1s, with the remaining elements playing a lesser role in genome plasticity... - Multiple fates of L1 retrotransposition intermediates in cultured human cellsNicolas Gilbert
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, 48109-0618, USA
Mol Cell Biol 25:7780-95. 2005..Indeed, the initial steps in L1 retrotransposition may define a host/parasite battleground that serves to limit the number of active L1s in the genome... - Unconventional translation of mammalian LINE-1 retrotransposonsReid S Alisch
Department of Human Genetics and Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109-0618, USA
Genes Dev 20:210-24. 2006..Similar data obtained from "natural" and codon optimized "synthetic" mouse L1s lead us to propose that ORF2 is translated by an unconventional termination/reinitiation mechanism... - Ribonucleoprotein particle formation is necessary but not sufficient for LINE-1 retrotranspositionDeanna A Kulpa
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-0618, USA
Hum Mol Genet 14:3237-48. 2005..Thus, these data indicate that RNP formation is important but not sufficient for L1 retrotransposition and suggest that ORF1p also may function at downstream steps in the L1 retrotransposition pathway... - A YY1-binding site is required for accurate human LINE-1 transcription initiationJyoti N Athanikar
Department of Human Genetics, The University of Michigan Medical School, Ann Arbor, MI 48109-0618, USA
Nucleic Acids Res 32:3846-55. 2004..Indeed, this sequence may explain the evolutionary success of LINE-1 by enabling full-length retrotransposed copies to undergo autonomous retrotransposition in subsequent generations... - Distinct mechanisms for trans-mediated mobilization of cellular RNAs by the LINE-1 reverse transcriptaseJose L Garcia Perez
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
Genome Res 17:602-11. 2007..Thus, we propose that the LINE-1-encoded reverse transcriptase can mediate the retrotransposition of non-L1 RNAs by distinct mechanisms... - DNA repair mediated by endonuclease-independent LINE-1 retrotranspositionTammy A Morrish
Department of Human Genetics, University of Michigan Medical School, 1241 E Catherine Street, Ann Arbor, Michigan 48105 0618, USA
Nat Genet 31:159-65. 2002..Thus, our results suggest that LINE-1s can integrate into DNA lesions, resulting in retrotransposon-mediated DNA repair in mammalian cells... - Characterization of a mutagenic B1 retrotransposon insertion in the jittery mouseNicolas Gilbert
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan, USA
Hum Mutat 24:9-13. 2004..Together, these data demonstrate that B1 retrotransposition is ongoing in the mouse genome and is consistent with the hypothesis that the reverse transcriptase and endonuclease encoded by LINE-1 elements mediate B1 mobility... - Endonuclease-independent LINE-1 retrotransposition at mammalian telomeresTammy A Morrish
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
Nature 446:208-12. 2007..Thus, we propose that EN(i) retrotransposition is an ancestral mechanism of RNA-mediated DNA repair associated with non-LTR retrotransposons that may have been used before the acquisition of an endonuclease domain... - A comprehensive analysis of recently integrated human Ta L1 elementsJeremy S Myers
Department of Biological Sciences, Biological Computation and Visualization Center, Louisiana State University, 202 Life Sciences Building, Baton Rouge, LA 70803, USA
Am J Hum Genet 71:312-26. 2002..One hundred fifteen (45%) of the Ta L1 elements were polymorphic with respect to insertion presence or absence and will serve as identical-by-descent markers for the study of human evolution... - Characterization of pre-insertion loci of de novo L1 insertionsStephen L Gasior
Tulane Cancer Center and Department of Epidemiology, Tulane University Health Sciences Center SL 66, 1430 Tulane Ave, New Orleans, LA 70112, United States
Gene 390:190-8. 2007.... - Cellular inhibitors of long interspersed element 1 and Alu retrotranspositionHal P Bogerd
Center for Virology and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 103:8780-5. 2006..These data suggest that the APOBEC3 protein family may have evolved, at least in part, to defend the integrity of the human genome against endogenous retrotransposons... - L1 retrotransposition in nondividing and primary human somatic cellsShuji Kubo
Department of Medicine, UCLA School of Medicine, 675 Charles E. Young Drive South, MRL-1551, Los Angeles, CA 90095-7019, USA
Proc Natl Acad Sci U S A 103:8036-41. 2006..Thus, these data indicate that L1 retrotransposition can occur in nondividing somatic cells... - Somatic mosaicism in neuronal precursor cells mediated by L1 retrotranspositionAlysson R Muotri
Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
Nature 435:903-10. 2005..Our data therefore indicate that neuronal genomes might not be static, but some might be mosaic because of de novo L1 retrotransposition events... - Mobile elements and mammalian genome evolutionPrescott L Deininger
Department of Environmental Health Sciences, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
Curr Opin Genet Dev 13:651-8. 2003.... - Introduction for the Gene special issue dedicated to the meeting "Genomic impact of eukaryotic transposable elements" at AsilomarMark A Batzer
Gene 390:1-2. 2007 - Of man in miceJyoti N Athanikar
Nat Genet 32:562-3. 2002
Research Grants
- Line-1 Retrotransposition in Human Embryonic Stem CellsJohn V Moran; Fiscal Year: 2010..An understanding of the basic molecular processes that impact genome integrity in hES cells is important to evaluate since these cells ultimately may be used therapeutically to treat a wide-range of diseases. ..
- Line-1 Retrotransposition in Human Embryonic Stem CellsJohn Moran; Fiscal Year: 2009..An understanding of the basic molecular processes that impact genome integrity in hES cells is important to evaluate since these cells ultimately may be used therapeutically to treat a wide-range of diseases. ..
- Mobile Elements in the Mammalian GenomeJohn Moran; Fiscal Year: 2007..In this proposal, we request funding for a second meeting on Mobile Elements in Mammalian Genomes to take place in June 2007. ..
- Genetic and Molecular Analysis of Human LINE-1 RetrotranspositionJohn Moran; Fiscal Year: 2007..The long-term goal of this project is to gain a fundamental understanding of how L1 retrotransportation contributes to human disease and genetic diversity. ..
- GENET & MOLEC ANALYSIS OF HUMAN LINE1 RETROTRANSPOSITIONJohn Moran; Fiscal Year: 2004..abstract_text> ..
- Genetic and Molecular Analyses of Human LINE-1 RetrotranspositionJohn V Moran; Fiscal Year: 2010..Through these studies, we will learn more about how this intriguing family of repetitive DNA sequences contributes to the structure and function of the human genome. ..