John Moran

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi request reprint Exon shuffling by L1 retrotransposition
    J V Moran
    Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104 6145 USA
    Science 283:1530-4. 1999
  2. ncbi request reprint Cis-preferential LINE-1 reverse transcriptase activity in ribonucleoprotein particles
    Deanna A Kulpa
    Department of Human Genetics 1241 E Catherine St, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
    Nat Struct Mol Biol 13:655-60. 2006
  3. pmc Genomic impact of eukaryotic transposable elements
    Irina R Arkhipova
    Institute of Experimental Pathology, ZMBE, University of Munster, Munster D 48149, Germany
    Mob DNA 3:19. 2012
  4. pmc Allelic heterogeneity in LINE-1 retrotransposition activity
    Sheila M Lutz
    Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109 0618, USA
    Am J Hum Genet 73:1431-7. 2003
  5. ncbi request reprint Human L1 retrotransposition: insights and peculiarities learned from a cultured cell retrotransposition assay
    J V Moran
    Department of Human Genetics and Internal Medicine, The University of Michigan Medical School, Ann Arbor 48109 0618, USA
    Genetica 107:39-51. 1999
  6. ncbi request reprint Multiple forms of genetic instability within a 2-Mb chromosomal segment of 3q26.3-q27 are associated with development of esophageal adenocarcinoma
    Lin Lin
    Department of Surgery Thoracic Section, University of Michigan Medical School, B560 MSRB2, Box 0686, Ann Arbor, MI 48109, USA
    Genes Chromosomes Cancer 45:319-31. 2006
  7. pmc Selective inhibition of Alu retrotransposition by APOBEC3G
    Amy E Hulme
    Department of Human Genetics, The University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Gene 390:199-205. 2007
  8. ncbi request reprint LINE-1 retrotransposition in human embryonic stem cells
    Jose L Garcia-Perez
    Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, 1241 E Catherine Street, Ann Arbor, MI 48109, USA
    Hum Mol Genet 16:1569-77. 2007
  9. pmc LINE-1 retrotransposition activity in human genomes
    Christine R Beck
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Cell 141:1159-70. 2010
  10. pmc Hot L1s account for the bulk of retrotransposition in the human population
    Brook Brouha
    Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 100:5280-5. 2003

Collaborators

Detail Information

Publications29

  1. ncbi request reprint Exon shuffling by L1 retrotransposition
    J V Moran
    Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104 6145 USA
    Science 283:1530-4. 1999
    ..Thus, retrotransposition-competent L1s provide a vehicle to mobilize non-L1 sequences, such as exons or promoters, into existing genes and may represent a general mechanism for the evolution of new genes...
  2. ncbi request reprint Cis-preferential LINE-1 reverse transcriptase activity in ribonucleoprotein particles
    Deanna A Kulpa
    Department of Human Genetics 1241 E Catherine St, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
    Nat Struct Mol Biol 13:655-60. 2006
    ..We further demonstrate that ORF2p preferentially uses its encoding RNA as a template for reverse transcription. Thus, our data provide the first biochemical evidence supporting the cis-preferential action of the L1 reverse transcriptase...
  3. pmc Genomic impact of eukaryotic transposable elements
    Irina R Arkhipova
    Institute of Experimental Pathology, ZMBE, University of Munster, Munster D 48149, Germany
    Mob DNA 3:19. 2012
    ..The success of the meeting reflects the important role of Repbase in comparative genomic studies, and emphasizes the need for close interactions between experimental and computational biologists in the years to come...
  4. pmc Allelic heterogeneity in LINE-1 retrotransposition activity
    Sheila M Lutz
    Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109 0618, USA
    Am J Hum Genet 73:1431-7. 2003
    ..2A retrotransposition efficiency. Thus, common L1 alleles can vary widely in their retrotransposition potential. We propose that such allelic heterogeneity can influence the potential L1 mutational load present in an individual genome...
  5. ncbi request reprint Human L1 retrotransposition: insights and peculiarities learned from a cultured cell retrotransposition assay
    J V Moran
    Department of Human Genetics and Internal Medicine, The University of Michigan Medical School, Ann Arbor 48109 0618, USA
    Genetica 107:39-51. 1999
    ..In general, I will limit the discussion to studies conducted on human L1s. However, interesting parallels to rodent L1s and other non-LTR retrotransposons also will be discussed...
  6. ncbi request reprint Multiple forms of genetic instability within a 2-Mb chromosomal segment of 3q26.3-q27 are associated with development of esophageal adenocarcinoma
    Lin Lin
    Department of Surgery Thoracic Section, University of Michigan Medical School, B560 MSRB2, Box 0686, Ann Arbor, MI 48109, USA
    Genes Chromosomes Cancer 45:319-31. 2006
    ..Thus, the fine dissection of a 2-Mb amplified DNA segment in 3q26.3-q27 in EA revealed multiple genetic alterations that had occurred sequentially and/or concurrently during EA development...
  7. pmc Selective inhibition of Alu retrotransposition by APOBEC3G
    Amy E Hulme
    Department of Human Genetics, The University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Gene 390:199-205. 2007
    ..These data demonstrate a differential restriction of L1 and Alu retrotransposition by APOBEC3G, and suggest that the Alu ribonucleoprotein complex may be targeted by APOBEC3G...
  8. ncbi request reprint LINE-1 retrotransposition in human embryonic stem cells
    Jose L Garcia-Perez
    Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, 1241 E Catherine Street, Ann Arbor, MI 48109, USA
    Hum Mol Genet 16:1569-77. 2007
    ..Thus, these data suggest that LINE-1 retrotransposition events may occur during early stages of human development...
  9. pmc LINE-1 retrotransposition activity in human genomes
    Christine R Beck
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Cell 141:1159-70. 2010
    ..Therefore, these results suggest that hot L1s are more abundant in the human population than previously appreciated, and that ongoing L1 retrotransposition continues to be a major source of interindividual genetic variation...
  10. pmc Hot L1s account for the bulk of retrotransposition in the human population
    Brook Brouha
    Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 100:5280-5. 2003
    ..Thus, our data indicate that most L1 retrotransposition in the human population stems from hot L1s, with the remaining elements playing a lesser role in genome plasticity...
  11. pmc ATLAS: a system to selectively identify human-specific L1 insertions
    Richard M Badge
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Am J Hum Genet 72:823-38. 2003
    ..Thus, ATLAS provides a simple, high-throughput means to assess genetic variation associated with L1 retrotransposons...
  12. pmc Epigenetic silencing of engineered L1 retrotransposition events in human embryonic carcinoma cells
    Jose L Garcia-Perez
    Department of Human Genetics, 1241 East Catherine Street, University of Michigan Medical School, Ann Arbor, Michigan 48109 5618, USA
    Nature 466:769-73. 2010
    ..Thus, our data indicate that ECs differ from many differentiated cells in their ability to silence reporter genes delivered by L1 retrotransposition...
  13. ncbi request reprint Genomic deletions created upon LINE-1 retrotransposition
    Nicolas Gilbert
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Cell 110:315-25. 2002
    ..Thus, our data demonstrate multiple pathways for L1 integration in cultured cells, and show that L1 is not simply an insertional mutagen, but that its retrotransposition can result in significant deletions of genomic sequence...
  14. pmc Unconventional translation of mammalian LINE-1 retrotransposons
    Reid S Alisch
    Department of Human Genetics and Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
    Genes Dev 20:210-24. 2006
    ..Similar data obtained from "natural" and codon optimized "synthetic" mouse L1s lead us to propose that ORF2 is translated by an unconventional termination/reinitiation mechanism...
  15. ncbi request reprint Ribonucleoprotein particle formation is necessary but not sufficient for LINE-1 retrotransposition
    Deanna A Kulpa
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109 0618, USA
    Hum Mol Genet 14:3237-48. 2005
    ..Thus, these data indicate that RNP formation is important but not sufficient for L1 retrotransposition and suggest that ORF1p also may function at downstream steps in the L1 retrotransposition pathway...
  16. pmc Multiple fates of L1 retrotransposition intermediates in cultured human cells
    Nicolas Gilbert
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, 48109 0618, USA
    Mol Cell Biol 25:7780-95. 2005
    ..Indeed, the initial steps in L1 retrotransposition may define a host/parasite battleground that serves to limit the number of active L1s in the genome...
  17. pmc Distinct mechanisms for trans-mediated mobilization of cellular RNAs by the LINE-1 reverse transcriptase
    Jose L Garcia-Perez
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
    Genome Res 17:602-11. 2007
    ..Thus, we propose that the LINE-1-encoded reverse transcriptase can mediate the retrotransposition of non-L1 RNAs by distinct mechanisms...
  18. pmc A YY1-binding site is required for accurate human LINE-1 transcription initiation
    Jyoti N Athanikar
    Department of Human Genetics, The University of Michigan Medical School, Ann Arbor, MI 48109 0618, USA
    Nucleic Acids Res 32:3846-55. 2004
    ..Indeed, this sequence may explain the evolutionary success of LINE-1 by enabling full-length retrotransposed copies to undergo autonomous retrotransposition in subsequent generations...
  19. ncbi request reprint DNA repair mediated by endonuclease-independent LINE-1 retrotransposition
    Tammy A Morrish
    Department of Human Genetics, University of Michigan Medical School, 1241 E Catherine Street, Ann Arbor, Michigan 48105 0618, USA
    Nat Genet 31:159-65. 2002
    ..Thus, our results suggest that LINE-1s can integrate into DNA lesions, resulting in retrotransposon-mediated DNA repair in mammalian cells...
  20. ncbi request reprint Endonuclease-independent LINE-1 retrotransposition at mammalian telomeres
    Tammy A Morrish
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48109 0618, USA
    Nature 446:208-12. 2007
    ..Thus, we propose that EN(i) retrotransposition is an ancestral mechanism of RNA-mediated DNA repair associated with non-LTR retrotransposons that may have been used before the acquisition of an endonuclease domain...
  21. ncbi request reprint Characterization of a mutagenic B1 retrotransposon insertion in the jittery mouse
    Nicolas Gilbert
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan, USA
    Hum Mutat 24:9-13. 2004
    ..Together, these data demonstrate that B1 retrotransposition is ongoing in the mouse genome and is consistent with the hypothesis that the reverse transcriptase and endonuclease encoded by LINE-1 elements mediate B1 mobility...
  22. pmc Characterization of pre-insertion loci of de novo L1 insertions
    Stephen L Gasior
    Tulane Cancer Center and Department of Epidemiology, Tulane University Health Sciences Center SL 66, 1430 Tulane Ave, New Orleans, LA 70112, United States
    Gene 390:190-8. 2007
    ....
  23. pmc Cellular inhibitors of long interspersed element 1 and Alu retrotransposition
    Hal P Bogerd
    Center for Virology and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 103:8780-5. 2006
    ..These data suggest that the APOBEC3 protein family may have evolved, at least in part, to defend the integrity of the human genome against endogenous retrotransposons...
  24. pmc L1 retrotransposition in nondividing and primary human somatic cells
    Shuji Kubo
    Department of Medicine, UCLA School of Medicine, 675 Charles E Young Drive South, MRL 1551, Los Angeles, CA 90095 7019, USA
    Proc Natl Acad Sci U S A 103:8036-41. 2006
    ..Thus, these data indicate that L1 retrotransposition can occur in nondividing somatic cells...
  25. ncbi request reprint Mobile elements and mammalian genome evolution
    Prescott L Deininger
    Department of Environmental Health Sciences, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Curr Opin Genet Dev 13:651-8. 2003
    ....
  26. ncbi request reprint Somatic mosaicism in neuronal precursor cells mediated by L1 retrotransposition
    Alysson R Muotri
    Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 435:903-10. 2005
    ..Our data therefore indicate that neuronal genomes might not be static, but some might be mosaic because of de novo L1 retrotransposition events...
  27. pmc A comprehensive analysis of recently integrated human Ta L1 elements
    Jeremy S Myers
    Department of Biological Sciences, Biological Computation and Visualization Center, Louisiana State University, 202 Life Sciences Building, Baton Rouge, LA 70803, USA
    Am J Hum Genet 71:312-26. 2002
    ..One hundred fifteen (45%) of the Ta L1 elements were polymorphic with respect to insertion presence or absence and will serve as identical-by-descent markers for the study of human evolution...
  28. ncbi request reprint Introduction for the Gene special issue dedicated to the meeting "Genomic impact of eukaryotic transposable elements" at Asilomar
    Mark A Batzer
    Gene 390:1-2. 2007
  29. ncbi request reprint Of man in mice
    Jyoti N Athanikar
    Nat Genet 32:562-3. 2002

Research Grants15

  1. Line-1 Retrotransposition in Human Embryonic Stem Cells
    John V Moran; Fiscal Year: 2010
    ..An understanding of the basic molecular processes that impact genome integrity in hES cells is important to evaluate since these cells ultimately may be used therapeutically to treat a wide-range of diseases. ..
  2. Line-1 Retrotransposition in Human Embryonic Stem Cells
    John Moran; Fiscal Year: 2009
    ..An understanding of the basic molecular processes that impact genome integrity in hES cells is important to evaluate since these cells ultimately may be used therapeutically to treat a wide-range of diseases. ..
  3. Mobile Elements in the Mammalian Genome
    John Moran; Fiscal Year: 2007
    ..In this proposal, we request funding for a second meeting on Mobile Elements in Mammalian Genomes to take place in June 2007. ..
  4. Genetic and Molecular Analysis of Human LINE-1 Retrotransposition
    John Moran; Fiscal Year: 2007
    ..The long-term goal of this project is to gain a fundamental understanding of how L1 retrotransportation contributes to human disease and genetic diversity. ..
  5. GENET & MOLEC ANALYSIS OF HUMAN LINE1 RETROTRANSPOSITION
    John Moran; Fiscal Year: 2004
    ..abstract_text> ..
  6. Genetic and Molecular Analyses of Human LINE-1 Retrotransposition
    John V Moran; Fiscal Year: 2010
    ..Through these studies, we will learn more about how this intriguing family of repetitive DNA sequences contributes to the structure and function of the human genome. ..