GRAHAM MORAN

Summary

Affiliation: University of Wisconsin-Milwaukee
Country: USA

Publications

  1. doi request reprint 4-Hydroxyphenylpyruvate dioxygenase and hydroxymandelate synthase: exemplars of the α-keto acid dependent oxygenases
    Graham R Moran
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 N Cramer St, Milwaukee, WI 53211 3209, United States Electronic address
    Arch Biochem Biophys 544:58-68. 2014
  2. ncbi request reprint 4-Hydroxyphenylpyruvate dioxygenase
    Graham R Moran
    Department of Chemistry and Biochemistry University of Wisconsin Milwaukee, 3210 N Cramer Street, Milwaukee, WI 53211 3029, USA
    Arch Biochem Biophys 433:117-28. 2005
  3. ncbi request reprint Interaction of (4-hydroxyphenyl)pyruvate dioxygenase with the specific inhibitor 2-[2-nitro-4-(trifluoromethyl)benzoyl]-1,3-cyclohexanedione
    Michael Kavana
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 42:10238-45. 2003
  4. ncbi request reprint Accumulation of multiple intermediates in the catalytic cycle of (4-hydroxyphenyl)pyruvate dioxygenase from Streptomyces avermitilis
    Kayunta Johnson-Winters
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 44:7189-99. 2005
  5. ncbi request reprint (4-Hydroxyphenyl)pyruvate dioxygenase from Streptomyces avermitilis: the basis for ordered substrate addition
    Kayunta Johnson-Winters
    Department of Chemistry, University of Wisconsin Milwaukee, 3210 N Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 42:2072-80. 2003
  6. ncbi request reprint Kinetic analysis of human homogentisate 1,2-dioxygenase
    Alphonso A Amaya
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, Milwaukee, WI 53211 3029, USA
    Arch Biochem Biophys 421:135-42. 2004
  7. doi request reprint The rate-limiting catalytic steps of hydroxymandelate synthase from Amycolatopsis orientalis
    Panqing He
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 49:1998-2007. 2010
  8. doi request reprint Kynurenine 3-monooxygenase from Pseudomonas fluorescens: substrate-like inhibitors both stimulate flavin reduction and stabilize the flavin-peroxo intermediate yet result in the production of hydrogen peroxide
    Karen R Crozier-Reabe
    Department of Chemistry and Biochemistry, University of Wisconsin, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 47:12420-33. 2008
  9. ncbi request reprint Catalytic, noncatalytic, and inhibitory phenomena: kinetic analysis of (4-hydroxyphenyl)pyruvate dioxygenase from Arabidopsis thaliana
    Vincent M Purpero
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 N Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 45:6044-55. 2006
  10. doi request reprint Product analysis and inhibition studies of a causative Asn to Ser variant of 4-hydroxyphenylpyruvate dioxygenase suggest a simple route to the treatment of Hawkinsinuria
    June M Brownlee
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 49:7218-26. 2010

Research Grants

Collaborators

  • Robert Phillips
  • Edward I Solomon
  • P F Fitzpatrick
  • Michael L Neidig
  • Michael Kavana
  • Panqing He
  • Kayunta Johnson-Winters
  • June M Brownlee
  • Vincent M Purpero
  • Karen R Crozier-Reabe
  • Karen R Crozier
  • Vincent Purpero
  • Karen E Keith
  • Oliver W Choroba
  • Jonathan B Spencer
  • Andrea Decker
  • Alphonso A Amaya
  • S Colette Daubner
  • Brian Heinz
  • John A Conrad
  • Judith Bates
  • Miguel A Valvano
  • Lauren Killip
  • Fanglu Huang
  • Christina D Brown
  • Natasha Farrington
  • David H T Harrison
  • Kotanee T Brzezinski
  • Tamara Nelson

Detail Information

Publications21

  1. doi request reprint 4-Hydroxyphenylpyruvate dioxygenase and hydroxymandelate synthase: exemplars of the α-keto acid dependent oxygenases
    Graham R Moran
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 N Cramer St, Milwaukee, WI 53211 3209, United States Electronic address
    Arch Biochem Biophys 544:58-68. 2014
    ..It is not intended as an exhaustive compilation of all observations made for these enzymes but rather a condensed narrative that connects those studies that have advanced the understanding of the chemistry of both enzymes. ..
  2. ncbi request reprint 4-Hydroxyphenylpyruvate dioxygenase
    Graham R Moran
    Department of Chemistry and Biochemistry University of Wisconsin Milwaukee, 3210 N Cramer Street, Milwaukee, WI 53211 3029, USA
    Arch Biochem Biophys 433:117-28. 2005
    ..Interestingly, HPPD inhibitor/herbicide molecules act also as therapeutic agents for a number of debilitating and lethal inborn defects in tyrosine catabolism by preventing the accumulation of toxic metabolites...
  3. ncbi request reprint Interaction of (4-hydroxyphenyl)pyruvate dioxygenase with the specific inhibitor 2-[2-nitro-4-(trifluoromethyl)benzoyl]-1,3-cyclohexanedione
    Michael Kavana
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 42:10238-45. 2003
    ..Fe(II) that formed would readily oxidize in the presence of dioxygen. Consistent with this observation, EPR spectroscopy has shown that only 2% of the HPPD.Fe(II).NTBC complex forms an NO complex as compared to the holoenzyme...
  4. ncbi request reprint Accumulation of multiple intermediates in the catalytic cycle of (4-hydroxyphenyl)pyruvate dioxygenase from Streptomyces avermitilis
    Kayunta Johnson-Winters
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 44:7189-99. 2005
    ..6 s(-)(1). This final event exhibits a kinetic isotope effect of 2 with deuterium oxide as the solvent, consistent with a solvent isotope effect on V(max) of 2.6 observed in steady-state experiments...
  5. ncbi request reprint (4-Hydroxyphenyl)pyruvate dioxygenase from Streptomyces avermitilis: the basis for ordered substrate addition
    Kayunta Johnson-Winters
    Department of Chemistry, University of Wisconsin Milwaukee, 3210 N Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 42:2072-80. 2003
    ..This complex reacts with molecular oxygen with a second-order rate constant of 1.4 x 10(5) M(-)(1) s(-)(1) inducing the formation of an intermediate that decays at the catalytically relevant rate of 7.8 s(-)(1)...
  6. ncbi request reprint Kinetic analysis of human homogentisate 1,2-dioxygenase
    Alphonso A Amaya
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, Milwaukee, WI 53211 3029, USA
    Arch Biochem Biophys 421:135-42. 2004
    ..35x10(3) M(-1) s(-1) at 4 degrees C. The term ordered with respect to the addition of substrates is most descriptive as the rate of reduction of molecular oxygen must increase in the presence of HG to sustain the observed turnover number...
  7. doi request reprint The rate-limiting catalytic steps of hydroxymandelate synthase from Amycolatopsis orientalis
    Panqing He
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 49:1998-2007. 2010
    ....
  8. doi request reprint Kynurenine 3-monooxygenase from Pseudomonas fluorescens: substrate-like inhibitors both stimulate flavin reduction and stabilize the flavin-peroxo intermediate yet result in the production of hydrogen peroxide
    Karen R Crozier-Reabe
    Department of Chemistry and Biochemistry, University of Wisconsin, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 47:12420-33. 2008
    ..The oxidative half-reaction observed in the presence of saturating benzoylalanine or m-nitrobenzoylalanine also shows the accumulation of a peroxyflavin species that then decays to yield hydrogen peroxide without hydroxylation...
  9. ncbi request reprint Catalytic, noncatalytic, and inhibitory phenomena: kinetic analysis of (4-hydroxyphenyl)pyruvate dioxygenase from Arabidopsis thaliana
    Vincent M Purpero
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 N Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 45:6044-55. 2006
    ..Additional absorbance features at 365 and 670 nm accumulate during inactivation and give the inactivated enzyme a green color but has the same molecular mass as the active enzyme as determined by mass spectrometry...
  10. doi request reprint Product analysis and inhibition studies of a causative Asn to Ser variant of 4-hydroxyphenylpyruvate dioxygenase suggest a simple route to the treatment of Hawkinsinuria
    June M Brownlee
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 North Cramer Street, Milwaukee, Wisconsin 53211 3029, USA
    Biochemistry 49:7218-26. 2010
    ..Moreover, the extreme stability of the HPPD.NTBC complex suggests that NTBC would be a potent therapeutic for Hawkinisinuria that would alleviate the extreme frailty experienced in the early life period...
  11. doi request reprint We two alone will sing: the two-substrate alpha-keto acid-dependent oxygenases
    Panqing He
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 N Cramer Street, Milwaukee, WI 53211 3029, USA
    Curr Opin Chem Biol 13:443-50. 2009
    ....
  12. ncbi request reprint Heterologous expression and purification of kynurenine-3-monooxygenase from Pseudomonas fluorescens strain 17400
    Karen R Crozier
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 N Cramer St, Milwaukee, WI 53211 3029, USA
    Protein Expr Purif 51:324-33. 2007
    ..PfKMO is offered as prototypical bacterial form of the enzyme to serve as a viable platform on which to base future KMO studies...
  13. ncbi request reprint The diverse and pervasive chemistries of the alpha-keto acid dependent enzymes
    Vincent Purpero
    Department of Chemistry and Biochemistry, University of Wisconsin Milwaukee, 3210 N Cramer Street, Milwaukee, WI 53211 3029, USA
    J Biol Inorg Chem 12:587-601. 2007
    ..In this minireview we present what is known of the alpha-keto acid dependent enzymes and offer an argument that the chemistry that is unique to each enzyme occurs only after the production of a pivotal ferryl-oxo intermediate...
  14. ncbi request reprint Spectroscopic and electronic structure studies of the role of active site interactions in the decarboxylation reaction of alpha-keto acid-dependent dioxygenases
    Michael L Neidig
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    J Inorg Biochem 100:2108-16. 2006
    ..Such an interaction would disfavor the decarboxylation reaction by stabilizing electron density on the carboxylate group such that the oxidative cleavage to yield CO(2) is disfavored...
  15. ncbi request reprint Role of tryptophan hydroxylase phe313 in determining substrate specificity
    S Colette Daubner
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843, USA
    Biochem Biophys Res Commun 292:639-41. 2002
    ....
  16. ncbi request reprint Spectroscopic and computational studies of NTBC bound to the non-heme iron enzyme (4-hydroxyphenyl)pyruvate dioxygenase: active site contributions to drug inhibition
    Michael L Neidig
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Biochem Biophys Res Commun 338:206-14. 2005
    ....
  17. ncbi request reprint Structure of the ferrous form of (4-hydroxyphenyl)pyruvate dioxygenase from Streptomyces avermitilis in complex with the therapeutic herbicide, NTBC
    June M Brownlee
    Department of Biochemistry and Molecular Biology, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, Illinois 60064, USA
    Biochemistry 43:6370-7. 2004
    ....
  18. ncbi request reprint CD and MCD studies of the non-heme ferrous active site in (4-hydroxyphenyl)pyruvate dioxygenase: correlation between oxygen activation in the extradiol and alpha-KG-dependent dioxygenases
    Michael L Neidig
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Am Chem Soc 126:4486-7. 2004
    ....
  19. pmc Burkholderia cenocepacia C5424 produces a pigment with antioxidant properties using a homogentisate intermediate
    Karen E Keith
    Department of Microbiology and Immunology, Siebens Drake Research Institute, University of Western Ontario, London, Ontario, N6A 5C1, Canada
    J Bacteriol 189:9057-65. 2007
    ..cenocepacia C5424 is a pyomelanin synthesized from an HGA intermediate that is capable of protecting the organism from in vitro and in vivo sources of oxidative stress...
  20. pmc Spectroscopic and electronic structure studies of aromatic electrophilic attack and hydrogen-atom abstraction by non-heme iron enzymes
    Michael L Neidig
    Department of Chemistry, Stanford University, CA 94306, USA
    Proc Natl Acad Sci U S A 103:12966-73. 2006
    ..Elongation of the Fe(IV) O bond in the transition state leads to dominant Fe(III) O(*-) character, which significantly contributes to the reactivity with either the aromatic pi-system or the C H sigma-bond...

Research Grants5

  1. 4-Hydroxyphenylpyruvate Dioxygenase and Tyrosinemia
    GRAHAM MORAN; Fiscal Year: 2005
    ....