Melissa J Moore

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. pmc The biflavonoid isoginkgetin is a general inhibitor of Pre-mRNA splicing
    Kristine O'Brien
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    J Biol Chem 283:33147-54. 2008
  2. doi request reprint Pre-mRNA processing reaches back to transcription and ahead to translation
    Melissa J Moore
    Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Cell 136:688-700. 2009
  3. pmc Mutational analysis of human eIF4AIII identifies regions necessary for exon junction complex formation and nonsense-mediated mRNA decay
    Toshiharu Shibuya
    Howard Hughes Medical Institute, Department of Biochemistry, MS009, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    RNA 12:360-74. 2006
  4. pmc Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core
    Thomas Ø Tange
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    RNA 11:1869-83. 2005
  5. pmc Ordered and dynamic assembly of single spliceosomes
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 331:1289-95. 2011
  6. ncbi request reprint The EJC factor eIF4AIII modulates synaptic strength and neuronal protein expression
    Corinna Giorgi
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA
    Cell 130:179-91. 2007
  7. ncbi request reprint The three-dimensional arcitecture of the EJC core
    M Elizabeth Stroupe
    Howard Hughes Medical Institute, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    J Mol Biol 360:743-9. 2006
  8. pmc New insights into the spliceosome by single molecule fluorescence microscopy
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA
    Curr Opin Chem Biol 15:864-70. 2011
  9. ncbi request reprint Pre-mRNA splicing: awash in a sea of proteins
    Melissa S Jurica
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    Mol Cell 12:5-14. 2003
  10. pmc Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site
    Daniel J Crawford
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 110:6783-8. 2013

Research Grants

  1. Mammalian Pre-mRNA Splicing Mechanisms
    Melissa Moore; Fiscal Year: 2007
  2. EXON LIGATION--THE SPLICEOSOME AND GROUP 11 INTRONS
    Melissa Moore; Fiscal Year: 1999
  3. Mammalian Pre-mRNA Splicing Mechanisms
    Melissa Moore; Fiscal Year: 2009

Collaborators

Detail Information

Publications39

  1. pmc The biflavonoid isoginkgetin is a general inhibitor of Pre-mRNA splicing
    Kristine O'Brien
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    J Biol Chem 283:33147-54. 2008
    ..Our results suggest that splicing inhibition is the mechanistic basis of the anti-tumor activity of isoginkgetin. Thus, pre-mRNA splicing inhibitors may represent a novel avenue for development of new anti-cancer agents...
  2. doi request reprint Pre-mRNA processing reaches back to transcription and ahead to translation
    Melissa J Moore
    Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Cell 136:688-700. 2009
    ..The connections of pre-mRNA processing to upstream events in transcription and downstream events, including translation and mRNA decay, are elaborate, extensive, and remarkably interwoven...
  3. pmc Mutational analysis of human eIF4AIII identifies regions necessary for exon junction complex formation and nonsense-mediated mRNA decay
    Toshiharu Shibuya
    Howard Hughes Medical Institute, Department of Biochemistry, MS009, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    RNA 12:360-74. 2006
    ..Implications of these findings are discussed in the context of other recent results and a new structural model for human eIF4AIII based on the known crystal structure of Saccharomyces cerevisiae eIF4AI...
  4. pmc Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core
    Thomas Ø Tange
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    RNA 11:1869-83. 2005
    ..Using the same methodology, we further identified what appears to be the minimal stable EJC core, a heterotetrameric complex consisting of eIF4AIII, Magoh, Y14, and MLN51...
  5. pmc Ordered and dynamic assembly of single spliceosomes
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 331:1289-95. 2011
    ..This experimental strategy should prove widely useful for mechanistic analysis of other macromolecular machines in environments approaching the complexity of living cells...
  6. ncbi request reprint The EJC factor eIF4AIII modulates synaptic strength and neuronal protein expression
    Corinna Giorgi
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA
    Cell 130:179-91. 2007
    ....
  7. ncbi request reprint The three-dimensional arcitecture of the EJC core
    M Elizabeth Stroupe
    Howard Hughes Medical Institute, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    J Mol Biol 360:743-9. 2006
    ..Using known X-ray crystallographic structures and a model of three of the four components, we propose a model for complex assembly on RNA and explain how Y14:Magoh may influence eIF4AIII's RNA binding...
  8. pmc New insights into the spliceosome by single molecule fluorescence microscopy
    Aaron A Hoskins
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605, USA
    Curr Opin Chem Biol 15:864-70. 2011
    ..These methods complement ensemble measurements of splicing in vivo and in vitro to facilitate kinetic dissection of pre-mRNA splicing...
  9. ncbi request reprint Pre-mRNA splicing: awash in a sea of proteins
    Melissa S Jurica
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    Mol Cell 12:5-14. 2003
    ..Another recurring theme regards the dynamic nature of spliceosomal complexes, which may be even more intricate than previously thought...
  10. pmc Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5' splice site and branch site
    Daniel J Crawford
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 110:6783-8. 2013
    ..Separation of the sites of chemistry until very late in the splicing pathway may be crucial for preventing splicing at incorrect sites...
  11. ncbi request reprint The ever-increasing complexities of the exon junction complex
    Thomas Ø Tange
    Howard Hughes Medical Institute, Department of Biochemistry, MS009, Brandeis University, 415 South Street, Waltham, Massachusetts 02454, USA
    Curr Opin Cell Biol 16:279-84. 2004
    ..It is now apparent that the means of connection is a set of proteins, collectively called the exon junction complex, which are deposited as a consequence of splicing upstream of mRNA exon-exon junctions...
  12. doi request reprint Staufen1 senses overall transcript secondary structure to regulate translation
    Emiliano P Ricci
    1 Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA 2 RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, Massachusetts, USA 3 Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Nat Struct Mol Biol 21:26-35. 2014
    ..Our results also indicate that Stau1 regulates translation of transcription-regulatory proteins. ..
  13. ncbi request reprint From birth to death: the complex lives of eukaryotic mRNAs
    Melissa J Moore
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, 415 South Street, Waltham, MA 02454
    Science 309:1514-8. 2005
    ..Thus, a comprehensive understanding of eukaryotic gene expression requires an appreciation for how the lives of mRNAs are influenced by a wide array of diverse regulatory mechanisms...
  14. ncbi request reprint Three-dimensional structure of C complex spliceosomes by electron microscopy
    Melissa S Jurica
    Howard Hughes Medical Institute, Rosenstiel Basic Medical Sciences Research Center, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, Massachusetts 02454, USA
    Nat Struct Mol Biol 11:265-9. 2004
    ..The structure reveals a particle of dimensions 27 x 22 x 24 nm with a relatively open arrangement of three primary domains...
  15. pmc A convergence of rRNA and mRNA quality control pathways revealed by mechanistic analysis of nonfunctional rRNA decay
    Sarah E Cole
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Mol Cell 34:440-50. 2009
    ..We propose that 18S NRD and NGD are different observable outcomes of the same initiating event: a ribosome stalled inappropriately at a sense codon during translation elongation...
  16. ncbi request reprint Nuclear RNA turnover
    Melissa J Moore
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    Cell 108:431-4. 2002
    ..In mammalian cells, significantly more RNA is turned over in the nucleus than in the cytoplasm. However, only recently have we begun to understand the mechanisms and regulation of nuclear RNA decay...
  17. ncbi request reprint Capturing splicing complexes to study structure and mechanism
    Melissa S Jurica
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    Methods 28:336-45. 2002
    ..In this paper, we describe the purification in detail and discuss additional methods for trapping and analyzing other splicing complexes...
  18. ncbi request reprint The nuclear nurture and cytoplasmic nature of localized mRNPs
    Corinna Giorgi
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA
    Semin Cell Dev Biol 18:186-93. 2007
    ..In this review we focus on nuclear-acquired RNA-binding proteins and complexes that participate in determining the journey of localized mRNAs...
  19. ncbi request reprint How introns influence and enhance eukaryotic gene expression
    Hervé Le Hir
    Howard Hughes Medical Institute, Department of Biochemistry, MS009 Brandeis University, Waltham, MA 02454, USA
    Trends Biochem Sci 28:215-20. 2003
    ..A rudimentary understanding of these effects can prove invaluable to researchers interested in optimizing transgene expression in eukaryotic systems...
  20. pmc Visualizing the splicing of single pre-mRNA molecules in whole cell extract
    Daniel J Crawford
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02453, USA
    RNA 14:170-9. 2008
    ..This work provides a powerful new approach for elucidating the mechanisms of spliceosome function and demonstrates the feasibility of utilizing TIRF microscopy for biochemical studies of single molecules in highly complex environments...
  21. pmc Splicing enhances translation in mammalian cells: an additional function of the exon junction complex
    Ajit Nott
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Genes Dev 18:210-22. 2004
    ..Furthermore, the ability to stimulate translation when bound inside an open reading frame appears to be a general feature of factors required for NMD...
  22. pmc Association of ribosomal proteins with nascent transcripts in S. cerevisiae
    Patricia A Schroder
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454 9110, USA
    RNA 11:1521-9. 2005
    ..However, our data clearly indicate that this binding is independent of transcript translatability, so is therefore not indicative of nuclear translation...
  23. pmc 5' exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly
    Vienna L Reichert
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Massachusetts 02454, USA
    Genes Dev 16:2778-91. 2002
    ..Upon exon ligation, association of RNPS1, UAP56, and SRm160 is destabilized. In contrast, REF/Aly, Y14, and Magoh remain stably bound to spliced mRNA, indicating that these three proteins are components of the EJC core...
  24. pmc Effects of the U1C L13 mutation and temperature regulation of yeast commitment complex formation
    Hansen Du
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, MS008, 415 South Street, Waltham, MA 02454, USA
    Proc Natl Acad Sci U S A 101:14841-6. 2004
    ..A model is proposed to account for the L13 results...
  25. doi request reprint Introns in UTRs: why we should stop ignoring them
    Alicia A Bicknell
    Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
    Bioessays 34:1025-34. 2012
    ..Nonetheless, recent findings indicate that 5'- and 3'-UTR intron status is of significant functional consequence for the regulation of mammalian genes. Therefore these features should be ignored no longer...
  26. pmc The cellular EJC interactome reveals higher-order mRNP structure and an EJC-SR protein nexus
    Guramrit Singh
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 151:750-64. 2012
    ..Further, their protection of long mRNA stretches from nuclease digestion suggests that endogenous EJCs and SR proteins cooperate to promote mRNA packaging and compaction...
  27. ncbi request reprint A late-acting quality control process for mature eukaryotic rRNAs
    Frederick J LaRiviere
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    Mol Cell 24:619-26. 2006
    ..Thus, eukaryotes possess a quality-control mechanism, nonfunctional rRNA decay (NRD), capable of detecting and eliminating the rRNA component of mature ribosomes...
  28. ncbi request reprint eIF4AIII binds spliced mRNA in the exon junction complex and is essential for nonsense-mediated decay
    Toshiharu Shibuya
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Nat Struct Mol Biol 11:346-51. 2004
    ..Finally, a model is proposed by which eIF4AIII represents a new functional class of DExH/D box proteins that act as RNA clamps or 'place holders' for the sequence-independent attachment of additional factors to RNAs...
  29. pmc Interactions between eIF4AI and its accessory factors eIF4B and eIF4H
    Nadja Rozovsky
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02453, USA
    RNA 14:2136-48. 2008
    ..Our findings mirror and expand on recent biochemical and structural data regarding the interaction of eIF4AI's close relative eIF4AIII with its accessory protein MLN51...
  30. ncbi request reprint Spliceosome assembly and composition
    Arianne J Matlin
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Adv Exp Med Biol 623:14-35. 2007
    ..We also review recent advances regarding similarities and differences between the in vivo and in vitro assembly pathways, as well as proofreading mechanisms contributing to the fidelity of splice site selection...
  31. ncbi request reprint RNA events. No end to nonsense
    Melissa J Moore
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA
    Science 298:370-1. 2002
  32. doi request reprint Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy
    Inna Shcherbakova
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Cell Rep 5:151-65. 2013
    ..Thus, multiple pathways exist for assembling functional spliceosomes. These observations provide insight into the mechanisms of cross-intron coordination of initial spliceosome assembly...
  33. pmc ASPeak: an abundance sensitive peak detection algorithm for RIP-Seq
    Alper Kucukural
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605 and Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Bioinformatics 29:2485-6. 2013
    ..Our peak detection algorithm yields stringent and robust target sets enabling sensitive motif finding and downstream functional analyses...
  34. pmc A quantitative analysis of intron effects on mammalian gene expression
    Ajit Nott
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    RNA 9:607-17. 2003
    ..However, nucleocytoplasmic mRNA distribution and mRNA stability were largely unaffected. These findings were extended to two other introns in a TCR-beta minigene...
  35. doi request reprint RIPiT-Seq: a high-throughput approach for footprinting RNA:protein complexes
    Guramrit Singh
    Howard Hughes Medical Institute, RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, United States
    Methods 65:320-32. 2014
    ..It is therefore particularly suited for studying dynamic RNP assemblages whose composition evolves as gene expression proceeds. ..
  36. pmc Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis
    Melissa S Jurica
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    RNA 8:426-39. 2002
    ..These images represent an important first step toward attaining a comprehensive three-dimensional understanding of pre-mRNA splicing...
  37. pmc Torsin mediates primary envelopment of large ribonucleoprotein granules at the nuclear envelope
    Vahbiz Jokhi
    Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell Rep 3:988-95. 2013
    ....
  38. doi request reprint A long noncoding RNA mediates both activation and repression of immune response genes
    Susan Carpenter
    Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 341:789-92. 2013
    ..Collectively, these studies unveil a central role of lincRNA-Cox2 as a broad-acting regulatory component of the circuit that controls the inflammatory response. ..
  39. pmc An ancient transcription factor initiates the burst of piRNA production during early meiosis in mouse testes
    Xin Zhiguo Li
    Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 50:67-81. 2013
    ..This regulatory circuit, which can be detected in rooster testes, likely predates the divergence of birds and mammals...

Research Grants15

  1. Mammalian Pre-mRNA Splicing Mechanisms
    Melissa Moore; Fiscal Year: 2007
    ....
  2. EXON LIGATION--THE SPLICEOSOME AND GROUP 11 INTRONS
    Melissa Moore; Fiscal Year: 1999
    ..Together, these experiments should allow direct comparison of active site structure, 3' splice site recognition and mechanisms of catalysis by the spliceosome and both group II and group I self-splicing introns. ..
  3. Mammalian Pre-mRNA Splicing Mechanisms
    Melissa Moore; Fiscal Year: 2009
    ..abstract_text> ..