R T Moon

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint Teaching resource. Canonical Wnt/beta-catenin signaling
    Randall T Moon
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Sci STKE 2004:tr5. 2004
  2. pmc A small molecule inhibitor of beta-catenin/CREB-binding protein transcription [corrected]
    Katayoon H Emami
    Institute for Chemical Genomics, 600 Broadway, Seattle, WA 98122, USA
    Proc Natl Acad Sci U S A 101:12682-7. 2004
  3. ncbi request reprint Wnt/beta-catenin pathway
    Randall T Moon
    HHMI, Department of Pharmacology, Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Sci STKE 2005:cm1. 2005
  4. pmc Wnt5a control of cell polarity and directional movement by polarized redistribution of adhesion receptors
    Eric S Witze
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO80309, USA
    Science 320:365-9. 2008
  5. pmc When Wnts antagonize Wnts
    Gilbert Weidinger
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington, Box 357370, Seattle, WA 98195, USA
    J Cell Biol 162:753-5. 2003
  6. pmc Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos
    Laird C Sheldahl
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195, USA
    J Cell Biol 161:769-77. 2003
  7. pmc Stromelysin-1 and mesothelin are differentially regulated by Wnt-5a and Wnt-1 in C57mg mouse mammary epithelial cells
    Mary G Prieve
    Department of Pharmacology, Howard Hughes Medical Institute, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    BMC Dev Biol 3:2. 2003
  8. pmc Wnt signaling induces epithelial differentiation during cutaneous wound healing
    Carrie Fathke
    Department of Surgery, University of Washington School of Medicine, Seattle, WA 98195, USA
    BMC Cell Biol 7:4. 2006
  9. pmc A plasmid-based system for expressing small interfering RNA libraries in mammalian cells
    Ajamete Kaykas
    Howard Hughes Medical Institute, Department of Pharmacology, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195
    BMC Cell Biol 5:16. 2004
  10. ncbi request reprint WNTs modulate cell fate and behavior during vertebrate development
    R T Moon
    Howard Hughes Institute, University of Washington School of Medicine, Seattle 98195, USA
    Trends Genet 13:157-62. 1997

Research Grants

  1. Conference on Wnt and Beta Catenin Signaling
    Randall Moon; Fiscal Year: 2002

Detail Information

Publications108 found, 100 shown here

  1. ncbi request reprint Teaching resource. Canonical Wnt/beta-catenin signaling
    Randall T Moon
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Sci STKE 2004:tr5. 2004
    ..Thus, the presence of the ligand Wnt inactivates the kinase allowing accumulation of beta-catenin, which then translocates to the nucleus and acts as a transcriptional regulator...
  2. pmc A small molecule inhibitor of beta-catenin/CREB-binding protein transcription [corrected]
    Katayoon H Emami
    Institute for Chemical Genomics, 600 Broadway, Seattle, WA 98122, USA
    Proc Natl Acad Sci U S A 101:12682-7. 2004
    ..ICG-001 selectively induces apoptosis in transformed cells but not in normal colon cells, reduces in vitro growth of colon carcinoma cells, and is efficacious in the Min mouse and nude mouse xenograft models of colon cancer...
  3. ncbi request reprint Wnt/beta-catenin pathway
    Randall T Moon
    HHMI, Department of Pharmacology, Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Sci STKE 2005:cm1. 2005
    ..The pathway is linked to bone density syndromes and to neurodegenerative diseases, and the pathway may also be involved in the retinal disease familial exudative vitreoretinopathy...
  4. pmc Wnt5a control of cell polarity and directional movement by polarized redistribution of adhesion receptors
    Eric S Witze
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO80309, USA
    Science 320:365-9. 2008
    ..Thus, cell-autonomous mechanisms allow Wnt5a to control cell orientation, polarity, and directional movement in response to positional cues from chemokine gradients...
  5. pmc When Wnts antagonize Wnts
    Gilbert Weidinger
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington, Box 357370, Seattle, WA 98195, USA
    J Cell Biol 162:753-5. 2003
    ..These studies present the first genetic confirmation of the previous hypothesis that vertebrate Wnt signaling pathways can act in an antagonistic manner...
  6. pmc Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos
    Laird C Sheldahl
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195, USA
    J Cell Biol 161:769-77. 2003
    ..These data suggest that the Wnt-Ca2+ pathway utilizes Dsh, thereby implicating Dsh as a component of all reported Fz signaling pathways...
  7. pmc Stromelysin-1 and mesothelin are differentially regulated by Wnt-5a and Wnt-1 in C57mg mouse mammary epithelial cells
    Mary G Prieve
    Department of Pharmacology, Howard Hughes Medical Institute, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    BMC Dev Biol 3:2. 2003
    ..Many downstream target genes of the Wnt/beta-catenin pathway have been identified. In contrast, little is known about the Wnt/Ca2+ pathway and whether it regulates gene expression...
  8. pmc Wnt signaling induces epithelial differentiation during cutaneous wound healing
    Carrie Fathke
    Department of Surgery, University of Washington School of Medicine, Seattle, WA 98195, USA
    BMC Cell Biol 7:4. 2006
    ....
  9. pmc A plasmid-based system for expressing small interfering RNA libraries in mammalian cells
    Ajamete Kaykas
    Howard Hughes Medical Institute, Department of Pharmacology, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195
    BMC Cell Biol 5:16. 2004
    ..However, there are multiple limitations to using hairpin siRNA vectors including the observation that some are unstable in bacteria and are difficult to sequence...
  10. ncbi request reprint WNTs modulate cell fate and behavior during vertebrate development
    R T Moon
    Howard Hughes Institute, University of Washington School of Medicine, Seattle 98195, USA
    Trends Genet 13:157-62. 1997
    ....
  11. ncbi request reprint Teaching resource. Beta-catenin signaling and axis specification
    Randall T Moon
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Sci STKE 2004:tr6. 2004
    ..This animation would be useful in illustrating events that occur early in embryogenesis and how embryos become polarized as a consequence of localized signaling processes...
  12. ncbi request reprint From cortical rotation to organizer gene expression: toward a molecular explanation of axis specification in Xenopus
    R T Moon
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle 98195, USA
    Bioessays 20:536-45. 1998
    ..These and other data are considered in the context of how the initial polarization of the fertilized egg by the localized accumulation of beta-catenin establishes a range of subsequent dorsoventral asymmetries in the embryo...
  13. ncbi request reprint WNT and beta-catenin signalling: diseases and therapies
    Randall T Moon
    Howard Hughes Medical Institute, Department of Pharmacology, and the Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Nat Rev Genet 5:691-701. 2004
    ....
  14. ncbi request reprint The promise and perils of Wnt signaling through beta-catenin
    Randall T Moon
    Howard Hughes Medical Institute, Department of Pharmacology, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Science 296:1644-6. 2002
    ..The STKE Connections Maps for these pathways provide an important tool in accessing this large body of complex information...
  15. ncbi request reprint The maternal Xenopus beta-catenin signaling pathway, activated by frizzled homologs, induces goosecoid in a cell non-autonomous manner
    J D Brown
    Howard Hughes Medical Institute, Seattle, Washington, USA
    Dev Growth Differ 42:347-57. 2000
    ..These data demonstrate that maternal Frizzleds can activate the Wnt/beta-catenin pathway in Xenopus embryos, and that induction of a known downstream gene can occur in a cell non-autonomous manner...
  16. ncbi request reprint XCtBP is a XTcf-3 co-repressor with roles throughout Xenopus development
    M Brannon
    Department of Biochemistry, Howard Hughes Medical Institute, Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195 7350, USA
    Development 126:3159-70. 1999
    ..Our data support a role for XCtBP as a co-repressor throughout Xenopus development and indicate that XCtBP/G4A will be a useful tool in determining how XCtBP functions in various developmental processes...
  17. ncbi request reprint Induction of a secondary embryonic axis in zebrafish occurs following the overexpression of beta-catenin
    G M Kelly
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle 98195 7370, USA
    Mech Dev 53:261-73. 1995
    ..These data are consistent with the involvement of beta-catenin in a Wnt signaling pathway which is involved in mesoderm induction in zebrafish...
  18. ncbi request reprint Signal transduction through beta-catenin and specification of cell fate during embryogenesis
    J R Miller
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, Seattle 98195 7370, USA
    Genes Dev 10:2527-39. 1996
  19. ncbi request reprint Wnt4 affects morphogenesis when misexpressed in the zebrafish embryo
    A R Ungar
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195, USA
    Mech Dev 52:153-64. 1995
    ..Our data suggest that wnt4, like Xwnt-5A, inhibits cell movements, and that these Wnts define a functional class distinct from the class which includes Wnt-1, Xwnt-3A and Xwnt-8...
  20. ncbi request reprint Zebrafish wnt8 and wnt8b share a common activity but are involved in distinct developmental pathways
    G M Kelly
    Howard Hughes Medical Institute, Department of Pharmacology, Seattle, Washington, USA
    Development 121:1787-99. 1995
    ....
  21. ncbi request reprint Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning
    A C Lekven
    Department of Pharmacology and Center for Developmental Biology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle 98195, USA
    Dev Cell 1:103-14. 2001
    ..These data demonstrate that the zebrafish wnt8 locus is required during gastrulation to pattern both the mesoderm and the neural ectoderm properly...
  22. ncbi request reprint Antisense RNA inhibits expression of membrane skeleton protein 4.1 during embryonic development of Xenopus
    D H Giebelhaus
    Department of Pharmacology, School of Medicine, University of Washington, Seattle 98195
    Cell 53:601-15. 1988
    ..These data suggest that reduced expression of a single membrane skeleton protein is sufficient to perturb normal cellular interactions of the retina...
  23. ncbi request reprint Inhibition of protein kinase A phenocopies ectopic expression of hedgehog in the CNS of wild-type and cyclops mutant embryos
    A R Ungar
    Department of Pharmacology, University of Washington, Seattle 98195 7750, USA
    Dev Biol 178:186-91. 1996
    ....
  24. ncbi request reprint The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3
    C Yost
    Department of Biochemistry, University of Washington School of Medicine, Seattle, 98195 7370, USA
    Genes Dev 10:1443-54. 1996
    ..These studies provide a basis for understanding the interaction between Xgsk-3 and beta-catenin in the establishment of the dorsal-ventral axis in early Xenopus embryos...
  25. ncbi request reprint Overlapping expression of Xwnt-3A and Xwnt-1 in neural tissue of Xenopus laevis embryos
    S L Wolda
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    Dev Biol 155:46-57. 1993
    ..Last, the ectopic expression of Xwnt-3A induces the formation of a secondary axis at the anterior end of the embryo...
  26. ncbi request reprint A role for xGCNF in midbrain-hindbrain patterning in Xenopus laevis
    K Song
    Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Dev Biol 213:170-9. 1999
    ....
  27. pmc Expression of the poly(A)-binding protein during development of Xenopus laevis
    B D Zelus
    Department of Pharmacology, School of Medicine, University of Washington, Seattle 98915
    Mol Cell Biol 9:2756-60. 1989
    ..Sagata, K. Shiokawa, and K. Yamana, Dev. Biol. 77:431-448, 1980)...
  28. ncbi request reprint Protein kinase C is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner
    L C Sheldahl
    Howard Hughes Medical Institute, Department of Pharmacology, Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington, 98195, USA
    Curr Biol 9:695-8. 1999
    ..In agreement with other studies [1] [2] [3]. [6] [7] these data support the existence of multiple Wnt and Frizzled signaling pathways in vertebrates...
  29. ncbi request reprint Xwnt-5A: a maternal Wnt that affects morphogenetic movements after overexpression in embryos of Xenopus laevis
    R T Moon
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    Development 119:97-111. 1993
    ..The data are consistent with Xwnt-5A having the potential activity of modifying the morphogenetic movements of tissues...
  30. ncbi request reprint Maternal and embryonic expression of zebrafish lef1
    R I Dorsky
    HHMI and Department of Pharmacology, University of Washington, Seattle, WA 98195, USA
    Mech Dev 86:147-50. 1999
    ..In addition, we cloned two tcf3 genes and a homolog of tcf4, neither of which were strongly expressed in premigratory neural crest...
  31. ncbi request reprint Control of neural crest cell fate by the Wnt signalling pathway
    R I Dorsky
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, Seattle 98195, USA
    Nature 396:370-3. 1998
    ..We conclude that endogenous Wnt signalling normally promotes pigment-cell formation by medial crest cells and thereby contributes to the diversity of neural crest cell fates...
  32. ncbi request reprint Isolation of cDNAs partially encoding four Xenopus Wnt-1/int-1-related proteins and characterization of their transient expression during embryonic development
    J L Christian
    Department of Pharmacology, University of Washington Medical Center, Seattle 98195
    Dev Biol 143:230-4. 1991
    ..The temporal changes in expression of these transcripts imply a unique role for each Xwnt during embryogenesis...
  33. ncbi request reprint The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos
    Maiyon Park
    Howard Hughes Medical Institute, Department of Pharmacology and Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Nat Cell Biol 4:20-5. 2002
    ....
  34. ncbi request reprint The armadillo homologs beta-catenin and plakoglobin are differentially expressed during early development of Xenopus laevis
    A A DeMarais
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    Dev Biol 153:337-46. 1992
    ..The presence of multiple homologs of armadillo in Xenopus embryos and the differences in their patterns of expression suggest distinct roles for these proteins in processes affected by cell adhesion...
  35. pmc Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway
    R I Dorsky
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Genes Dev 14:158-62. 2000
    ..Mutation of Tcf/Lef sites abolishes Lef1 binding and reporter function in vivo. Wnt signaling therefore directly activates nacre, which in turn leads to pigment cell differentiation...
  36. pmc A beta-catenin/XTcf-3 complex binds to the siamois promoter to regulate dorsal axis specification in Xenopus
    M Brannon
    Department of Biochemistry, University of Washington School of Medicine, Seattle 98195 7350, USA
    Genes Dev 11:2359-70. 1997
    ....
  37. ncbi request reprint Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway
    Ken Ichi Takemaru
    Howard Hughes Medical Institute, Room K536C Health Sciences Building, Campus Box 357750, Department of Pharmacology, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Nature 422:905-9. 2003
    ..In addition, epistasis experiments indicate that chibby acts downstream of wingless and upstream of armadillo...
  38. ncbi request reprint Zebrafish mdk2, a novel secreted midkine, participates in posterior neurogenesis
    C Winkler
    Howard Hughes Medical Institute, Center for Developmental Biology, Seattle, Washington 98195 7750, USA
    Dev Biol 229:102-18. 2001
    ..These data support the hypothesis that mdk2 participates in posterior neural development in zebrafish...
  39. ncbi request reprint Wnt signaling and heterotrimeric G-proteins: strange bedfellows or a classic romance?
    C C Malbon
    Department of Pharmacology, State University of New York at Stony Brook, Stony Brook, New York 11794 8651, USA
    Biochem Biophys Res Commun 287:589-93. 2001
    ..The new data, consistent with the properties known for virtually all members of the G-protein-coupled receptors, reveal a more classic romance of signaling elements controlling aspects of early development...
  40. ncbi request reprint A second canon. Functions and mechanisms of beta-catenin-independent Wnt signaling
    Michael T Veeman
    Howard Hughes Medical Institute, Department of Pharmacology, Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Dev Cell 5:367-77. 2003
    ....
  41. ncbi request reprint Membrane skeleton protein 4.1 in developing Xenopus: expression in postmitotic cells of the retina
    M Spencer
    Department of Ophthalmology, University of Washington School of Medicine, Seattle 98195
    Dev Biol 139:279-91. 1990
    ..As these cell types have previously been shown to express spectrin, actin, and ankyrin, it is likely that the membrane skeleton of erythrocytes and retinal cells share functional similarities...
  42. pmc Positive and negative regulation of muscle cell identity by members of the hedgehog and TGF-beta gene families
    S J Du
    Howard Hughes Medical Institute, University of Washington, School of Medicine, Seattle 98195, USA
    J Cell Biol 139:145-56. 1997
    ..We propose that a Hedgehog signal first induces the formation of slow muscle precursor cells, and subsequent Hedgehog and TGF-beta signals exert competing positive and negative influences on the development of muscle pioneer cells...
  43. ncbi request reprint Environmental signals and cell fate specification in premigratory neural crest
    R I Dorsky
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington, Seattle, WA, USA
    Bioessays 22:708-16. 2000
    ..This system may therefore provide a good model for integration of multiple signaling pathways during embryonic cell fate specification...
  44. ncbi request reprint Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation
    Benjamin N R Cheyette
    Howard Hughes Medical Institute, Department of Pharmacology and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Dev Cell 2:449-61. 2002
    ..Depletion of maternal Dapper RNA from Xenopus embryos results in loss of notochord and head structures, demonstrating that Dapper is required for normal vertebrate development...
  45. ncbi request reprint Cloning and developmental expression in Xenopus laevis of seven additional members of the Wnt family
    S L Wolda
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    Oncogene 7:1941-7. 1992
    ..The temporal and spatial differences in expression suggest different roles for these new Xwnt family members in Xenopus development...
  46. ncbi request reprint Activation of rat frizzled-1 promotes Wnt signaling and differentiation of mouse F9 teratocarcinoma cells via pathways that require Galpha(q) and Galpha(o) function
    T Liu
    Department of Pharmacology, State University of New York, Stony Brook, New York 11794 8651, USA
    J Biol Chem 274:33539-44. 1999
    ....
  47. ncbi request reprint Expression of wnt10a in the central nervous system of developing zebrafish
    G M Kelly
    Department of Pharmacology SJ 30, University of Washington School of Medicine, Seattle 98195
    Dev Biol 158:113-21. 1993
    ..These patterns suggest roles for wnt10a in the regionalization of the brain...
  48. ncbi request reprint A transgenic Lef1/beta-catenin-dependent reporter is expressed in spatially restricted domains throughout zebrafish development
    Richard I Dorsky
    Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Dev Biol 241:229-37. 2002
    ..We conclude that our TOP-dGFP reporter line faithfully illustrates domains of beta-catenin activity and enables the identification of responsive cell populations...
  49. ncbi request reprint Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A
    D C Slusarski
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle 98195, USA
    Dev Biol 182:114-20. 1997
    ..These results suggest that intercellular signaling by a subset of vertebrate Wnts involves modulation of a intracellular Ca2+ signaling pathway, which may arise from phosphatidylinositol cycle activity...
  50. pmc A protein complex of SCRIB, NOS1AP and VANGL1 regulates cell polarity and migration, and is associated with breast cancer progression
    J N Anastas
    Molecular and Cellular Biology Graduate Program, University of Washington School of Medicine, Seattle, WA 98109, USA
    Oncogene 31:3696-708. 2012
    ..We also find that reduction of NOS1AP enhances anchorage-independent growth. Collectively these data point to the relevance of NOS1AP and SCRIB protein complexes in breast cancer...
  51. ncbi request reprint Reverse genetics in zebrafish
    A C Lekven
    Howard Hughes Medical Institute, Department of Pharmacology, Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Physiol Genomics 2:37-48. 2000
    ....
  52. ncbi request reprint Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes
    Joshua S Waxman
    Molecular and Cellular Biology Program, University of Washington School of Medicine, Seattle, WA 98195, USA
    Development 131:5909-21. 2004
    ..We conclude that two Dvl-associated paralogs, Dpr1 and Dpr2, participate in distinct Wnt-dependent developmental processes...
  53. ncbi request reprint Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis
    J L Christian
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    Development 111:1045-55. 1991
    ..These findings suggest that Xwnt-8 may be a secondary signalling agent which is produced in response to mesoderm-inducing factors and is involved in the early steps of mesodermal patterning...
  54. doi request reprint New regulators of Wnt/beta-catenin signaling revealed by integrative molecular screening
    Michael B Major
    Howard Hughes Medical Institute, Department of Pharmacology, Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Box 357370, Seattle, WA 98195, USA
    Sci Signal 1:ra12. 2008
    ..Using this methodology, we characterized AGGF1 as a nuclear chromatin-associated protein that participates in beta-catenin-mediated transcription in human colon cancer cells...
  55. ncbi request reprint The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation
    Stephane Angers
    Howard Hughes Medical Institute, University of Washington School of Medicine, Box 357370, Seattle, WA 98195, USA
    Nat Cell Biol 8:348-57. 2006
    ....
  56. pmc A Wnt survival guide: from flies to human disease
    Andy J Chien
    The University of Washington School of Medicine, Department of Medicine and the Institute for Stem Cell and Regenerative Medicine, Seattle, Washington 98109, USA
    J Invest Dermatol 129:1614-27. 2009
    ..The aim of this review is to provide a context on the Wnt field that will facilitate the interpretation and study of Wnt signaling in the context of human disease...
  57. doi request reprint Beta-catenin gets jaded and von Hippel-Lindau is to blame
    Jason D Berndt
    Howard Hughes Medical Institute, Department of Pharmacology and Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Box 357370, Seattle, WA 98109, USA
    Trends Biochem Sci 34:101-4. 2009
    ..Thus, the loss of VHL, as seen in clear cell renal cell carcinoma, could lead to tumor formation through beta-catenin de-repression...
  58. ncbi request reprint Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements
    Michael T Veeman
    Department of Pharmacology, Center for Developmental Biology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA 98195, USA
    Curr Biol 13:680-5. 2003
    ..Our results support the idea that a vertebrate PCP pathway regulates gastrulation movements and suggest that there is overlap between the PCP and Wnt/calcium pathways...
  59. doi request reprint Integrative analysis of genome-wide RNA interference screens
    Jason D Berndt
    Howard Hughes Medical Institute, Department of Pharmacology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA
    Sci Signal 2:pt4. 2009
    ..We present the ongoing evolution and new strategies for the integrative screening approach with respect to understanding and treating human disease...
  60. ncbi request reprint Generation of diversity in nonerythroid spectrins. Multiple polypeptides are predicted by sequence analysis of cDNAs encompassing the coding region of human nonerythroid alpha-spectrin
    R T Moon
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    J Biol Chem 265:4427-33. 1990
    ..As this heterogeneity in amino acid sequence occurs near domains of nonerythroid alpha-spectrin suggested to bind calcium or actin, it is possible that fibroblasts express functionally distinct isoforms of nonerythroid alpha-spectrin...
  61. pmc Wnt/β-catenin pathway regulates bone morphogenetic protein (BMP2)-mediated differentiation of dental follicle cells
    K G Silvério
    Institute for Stem Cells and Regenerative Medicine, University of Washington, School of Medicine, Seattle, WA, USA
    J Periodontal Res 47:309-19. 2012
    ....
  62. ncbi request reprint Wnt signaling: why is everything so negative?
    J D Brown
    University of Washington, Howard Hughes Medical Institute, Department of Pharmacology, Seattle, WA 98195, USA
    Curr Opin Cell Biol 10:182-7. 1998
    ....
  63. ncbi request reprint Wnt/beta-catenin regulation of the Sp1-related transcription factor sp5l promotes tail development in zebrafish
    Chris J Thorpe
    Howard Hughes Medical Institute, Department of Pharmacology and Center for Developmental Biology, Box 357750, University of Washington School of Medicine, Seattle, WA 98195, USA
    Development 132:1763-72. 2005
    ..These data place sp5l downstream of wnt3a and wnt8 in a Wnt/beta-catenin signaling pathway that controls tail development in zebrafish...
  64. ncbi request reprint The Sp1-related transcription factors sp5 and sp5-like act downstream of Wnt/beta-catenin signaling in mesoderm and neuroectoderm patterning
    Gilbert Weidinger
    Howard Hughes Medical Institute, Department of Pharmacology and Center for Developmental Biology, University of Washington School of Medicine, Box 357370, Seattle, Washington 98195, USA
    Curr Biol 15:489-500. 2005
    ..However, relatively little is known about Wnt target genes mediating these effects...
  65. ncbi request reprint Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling
    Michael B Major
    Howard Hughes Medical Institute, University of Washington School of Medicine, Box 357370, Seattle, WA 98195, USA
    Science 316:1043-6. 2007
    ..These data provide a possible mechanistic explanation for the tumor suppressor activity of WTX...
  66. ncbi request reprint nemo-like kinase is an essential co-activator of Wnt signaling during early zebrafish development
    Chris J Thorpe
    Howard Hughes Medical Institute, Department of Pharmacology and Center for Developmental Biology, Box 357750, University of Washington School of Medicine, Seattle, WA 98195, USA
    Development 131:2899-909. 2004
    ..Additionally, nlk strongly enhances convergent/extension phenotypes associated with wnt11/silberblick, suggesting a role in modulating cell movements as well as cell fate...
  67. pmc Modulation of the beta-catenin signaling pathway by the dishevelled-associated protein Hipk1
    Sarah H Louie
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington, United States of America
    PLoS ONE 4:e4310. 2009
    ..Dishevelled (Dsh/Dvl) is a multi-domain scaffold protein required for virtually all known Wnt signaling activities, raising interest in the identification and functions of Dsh-associated proteins...
  68. ncbi request reprint Reiterated Wnt signaling during zebrafish neural crest development
    Jessica L Lewis
    Molecular and Cellular Biology Program, University of Washington School of Medicine, Seattle, WA 98195, USA
    Development 131:1299-308. 2004
    ..Taken together, these results demonstrate that Wnt signals are critical for the initial induction of zebrafish neural crest and suggest that this signaling pathway plays reiterated roles in its development...
  69. ncbi request reprint Analysis of Xwnt-4 in embryos of Xenopus laevis: a Wnt family member expressed in the brain and floor plate
    L L McGrew
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    Development 115:463-73. 1992
    ..The specific pattern of expression following neural induction suggests that Xwnt-4 plays a role in the early patterning events responsible in the formation of the nervous system in Xenopus...
  70. ncbi request reprint The tuberin-hamartin complex negatively regulates beta-catenin signaling activity
    Baldwin C Mak
    Department of Surgery, University of Washington, School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 278:5947-51. 2003
    ..Our data suggest that hamartin and tuberin negatively regulate beta-catenin stability and activity by participating in the beta-catenin degradation complex...
  71. pmc Bili inhibits Wnt/beta-catenin signaling by regulating the recruitment of axin to LRP6
    Lorna S Kategaya
    Department of Pharmacology, Howard Hughes Medical Institute HHMI, University of Washington School of Medicine and Institute for Stem Cell and Regenerative Medicine, Seattle, WA, USA
    PLoS ONE 4:e6129. 2009
    ..Insights into how the Frizzled/LRP6 receptor complex receives, transduces and terminates Wnt signals will enhance our understanding of the control of the Wnt/ss-catenin pathway...
  72. doi request reprint Assaying beta-catenin/TCF transcription with beta-catenin/TCF transcription-based reporter constructs
    Travis L Biechele
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, WA, USA
    Methods Mol Biol 468:99-110. 2008
    ..Its enhanced sensitivity, increased dynamic range, and lentiviral platform provide a reporter system that will keep pace with the needs of scientists in the field...
  73. pmc WNTS and WNT receptors as therapeutic tools and targets in human disease processes
    Andy J Chien
    University of Washington, Department of Medicine, Division of Dermatology, Seattle 98195, USA
    Front Biosci 12:448-57. 2007
    ....
  74. doi request reprint Beta-catenin-independent Wnt pathways: signals, core proteins, and effectors
    Richard G James
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, WA, USA
    Methods Mol Biol 468:131-44. 2008
    ..Finally, during the course of our discussion, we highlight areas that require future research...
  75. pmc Purification and characterization of recombinant Xenopus poly(A)(+)-binding protein expressed in a baculovirus system
    R A Stambuk
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    Biochem J 287:761-6. 1992
    ..This analysis suggested that there is less than one PABP molecule available per PABP-binding site at early stages of development, and only a slight excess of PABP at later stages...
  76. pmc CTLA-4 is a direct target of Wnt/beta-catenin signaling and is expressed in human melanoma tumors
    Kavita V Shah
    Department of Pharmacology, University of Washington, Seattle, Washington 98195, USA
    J Invest Dermatol 128:2870-9. 2008
    ....
  77. pmc Activated Wnt/beta-catenin signaling in melanoma is associated with decreased proliferation in patient tumors and a murine melanoma model
    Andy J Chien
    Institute for Stem Cell and Regenerative Medicine, Department of Medicine, Division of Dermatology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 106:1193-8. 2009
    ..Our results may explain the observed loss of nuclear beta-catenin with melanoma progression in human tumors, which could reflect a dysregulation of cellular differentiation through a loss of homeostatic Wnt/beta-catenin signaling...
  78. pmc The CCN family member Wisp3, mutant in progressive pseudorheumatoid dysplasia, modulates BMP and Wnt signaling
    Yukio Nakamura
    Howard Hughes Medical Institute, Department of Genetics, and Center for Human Genetics, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    J Clin Invest 117:3075-86. 2007
    ..These data provide a biologic assay for Wisp3, reveal a role for Wisp3 during zebrafish cartilage development, and suggest that dysregulation of BMP and/or Wnt signaling contributes to cartilage failure in humans with PPD...
  79. doi request reprint Wnt/Fz signaling and the cytoskeleton: potential roles in tumorigenesis
    Shih Lei Lai
    Howard Hughes Medical Institute, Department of Pharmacology, Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
    Cell Res 19:532-45. 2009
    ....
  80. pmc Chibby promotes adipocyte differentiation through inhibition of beta-catenin signaling
    Feng Qian Li
    SUNY at Stony Brook, Department of Pharmacology, Stony Brook, NY 11794 8651, USA
    Mol Cell Biol 27:4347-54. 2007
    ..Our results therefore establish Cby as a novel proadipogenic factor required for adipocyte differentiation...
  81. ncbi request reprint Mutant Frizzled 4 associated with vitreoretinopathy traps wild-type Frizzled in the endoplasmic reticulum by oligomerization
    Ajamete Kaykas
    Howard Hughes Medical Institute, Department of Pharmacology, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Nat Cell Biol 6:52-8. 2004
    ..These data support the hypothesis that oligomerization of mutant and wild-type Fz proteins occurs in the endoplasmic reticulum and may explain the genetic dominance of this FEVR allele...
  82. pmc Common genetic variation within the low-density lipoprotein receptor-related protein 6 and late-onset Alzheimer's disease
    Giancarlo V De Ferrari
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 104:9434-9. 2007
    ..Our study unveils a genetic relationship between LRP6 and APOE and supports the hypothesis that altered Wnt/beta-catenin signaling may be involved in this neurodegenerative disease...
  83. ncbi request reprint Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery
    Stephane Angers
    Howard Hughes Medical Institute, University of Washington, School of Medicine, Box 357280, Seattle, Washington 98195, USA
    Nature 443:590-3. 2006
    ..Together, our structural and proteomic results reveal the structural mechanisms and molecular logic underlying the assembly and versatility of a new family of cullin-RING E3 complexes...
  84. pmc Wnt/beta-catenin signaling has an essential role in the initiation of limb regeneration
    Hitoshi Yokoyama
    Howard Hughes Medical Institute, Department of Pharmacology, Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, Box 357370, Seattle, WA 98195, USA
    Dev Biol 306:170-8. 2007
    ..We conclude that Wnt/beta-catenin signaling has an essential role during the early stages of limb regeneration, but is not absolutely required after blastema formation...
  85. ncbi request reprint Distinct Wnt signaling pathways have opposing roles in appendage regeneration
    Cristi L Stoick-Cooper
    Howard Hughes Medical Institute, Department of Pharmacology, Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
    Development 134:479-89. 2007
    ..These data suggest that Wnt/beta-catenin signaling promotes regeneration, whereas a distinct pathway activated by wnt5b acts in a negative-feedback loop to limit regeneration...
  86. doi request reprint Crystal structure of a full-length beta-catenin
    Yi Xing
    Department of Biological Structure, University of Washington School of Medicine, Seattle, WA 98195, USA
    Structure 16:478-87. 2008
    ..Our crystallographic and NMR studies also suggest that the unstructured N-terminal and C-terminal tails interact with the ordered armadillo repeat domain in a dynamic and variable manner...
  87. pmc Biphasic role for Wnt/beta-catenin signaling in cardiac specification in zebrafish and embryonic stem cells
    Shuichi Ueno
    Department of Pathology, Center for Cardiovascular Biology, University of Washington School of Medicine, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 104:9685-90. 2007
    ..Thus, Wnt/beta-catenin signaling promotes cardiac differentiation at early developmental stages and inhibits it later. Control of this pathway may promote derivation of cardiomyocytes for basic research and cell therapy applications...
  88. doi request reprint APC mutant zebrafish uncover a changing temporal requirement for wnt signaling in liver development
    Wolfram Goessling
    Stem Cell Program and Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute, HHMI, Harvard Medical School, Boston, MA 02115, USA
    Dev Biol 320:161-74. 2008
    ..These studies reveal an important and time-dependent role for wnt signaling during liver development and regeneration...
  89. ncbi request reprint The interaction of the Wnt and Notch pathways modulates natural killer versus T cell differentiation
    Keisuke Aoyama
    Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, D2 373, Seattle, Washington 98109, USA
    Stem Cells 25:2488-97. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  90. pmc The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling
    Tohru Ishitani
    Department of Molecular Biology, Graduate School of Science, Nagoya University, Chikusa ku, Japan
    Mol Cell Biol 23:131-9. 2003
    ..These results suggest that the TAK1-NLK MAPK cascade is activated by the noncanonical Wnt-5a/Ca(2+) pathway and antagonizes canonical Wnt/beta-catenin signaling...
  91. ncbi request reprint Kaiso/p120-catenin and TCF/beta-catenin complexes coordinately regulate canonical Wnt gene targets
    Jae Il Park
    Department of Biochemistry and Molecular Biology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Dev Cell 8:843-54. 2005
    ..These studies point to convergence of parallel p120-catenin/Kaiso and beta-catenin/TCF signaling pathways to regulate gene expression in vertebrate development and possibly carcinogenesis...
  92. ncbi request reprint Disruption of acvrl1 increases endothelial cell number in zebrafish cranial vessels
    Beth L Roman
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Development 129:3009-19. 2002
    ..Movies available on-line..
  93. ncbi request reprint Developmental biology: signalling polarity
    Randall T Moon
    Nature 417:239-40. 2002
  94. ncbi request reprint Prolonged in vivo gene silencing by electroporation-mediated plasmid delivery of small interfering RNA
    Daniel Eefting
    Gaubius Laboratory, TNO Quality of Life, 2301 CE Leiden, The Netherlands
    Hum Gene Ther 18:861-9. 2007
    ..Therefore, this application of RNA interference is a useful tool with which to investigate gene function and might be promising as a therapeutic tool for locally acting diseases such as restenosis or tumors...
  95. ncbi request reprint Functional genomic analysis of the Wnt-wingless signaling pathway
    Ramanuj DasGupta
    Department of Genetics, Howard Hughes Medical Institute HHMI, Harvard Medical School, New Research Building, No 339, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Science 308:826-33. 2005
    ..High-throughput RNAi screens in cultured cells, followed by functional analyses in model organisms, prove to be a rapid means of identifying regulators of signaling pathways implicated in development and disease...
  96. pmc The Wnt5A/protein kinase C pathway mediates motility in melanoma cells via the inhibition of metastasis suppressors and initiation of an epithelial to mesenchymal transition
    Samudra K Dissanayake
    Laboratory of Immunology, Gerontology Research Center, NIA, National Institutes of Health NIH, Baltimore, Maryland 21224, USA
    J Biol Chem 282:17259-71. 2007
    ..Furthermore, inhibition of PKC before Wnt5A treatment blocked Snail expression, implying that Wnt5A can potentiate melanoma metastasis via the induction of EMT in a PKC-dependent manner...
  97. ncbi request reprint Two tcf3 genes cooperate to pattern the zebrafish brain
    Richard I Dorsky
    Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84132, USA
    Development 130:1937-47. 2003
    ..In addition, Tcf3b has a second and unique role in the morphogenesis of rhombomere boundaries, indicating that it controls multiple aspects of brain development...
  98. ncbi request reprint Wnt1 and wnt10b function redundantly at the zebrafish midbrain-hindbrain boundary
    Arne C Lekven
    Department of Biology, Texas A and M University, 3258 TAMU, College Station, TX 77843 3258, USA
    Dev Biol 254:172-87. 2003
    ..1 or Fgf8; that is, in combination with mutant alleles of either of these loci, the morphological MHB is lost. Thus, wnt1 and wnt10b are required to maintain threshold levels of Pax2.1 and Fgf8 at the MHB...
  99. ncbi request reprint Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP
    Adriana Ahumada
    Department of Molecular Pharmacology, Diabetes and Metabolic Diseases Research Center, University Medical Center, SUNY Stony Brook, Stony Brook, NY 11794 8651, USA
    Science 298:2006-10. 2002
    ..Moreover, PDE inhibitors blocked Rfz2-induced calcium transients in zebrafish embryos. Thus, Frizzled-2 appears to couple to PDEs and calcium transients through G proteins...

Research Grants1

  1. Conference on Wnt and Beta Catenin Signaling
    Randall Moon; Fiscal Year: 2002
    ..Given the explosive growth in research in this field owing to its connection to various cancers, it is extremely timely to have a meeting with the joint focus on the roles of Wnt and B-catenin signaling in both development and disease. ..