Raymond Monnat

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint Generation of highly site-specific DNA double-strand breaks in human cells by the homing endonucleases I-PpoI and I-CreI
    R J Monnat
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    Biochem Biophys Res Commun 255:88-93. 1999
  2. pmc From broken to old: DNA damage, IGF1 endocrine suppression and aging
    Raymond J Monnat
    Department of Pathology, University of Washington, Box 357705 Seattle, WA 98195 7705, United States
    DNA Repair (Amst) 6:1386-90. 2007
  3. ncbi request reprint Werner syndrome protein--unwinding function to explain disease
    Raymond J Monnat
    Department of Pathology, University of Washington, Seattle, WA 98195, USA
    Sci Aging Knowledge Environ 2004:re3. 2004
  4. pmc Human RECQ helicases: roles in DNA metabolism, mutagenesis and cancer biology
    Raymond J Monnat
    Department of Pathology, University of Washington, Seattle, WA 98195 7705, USA
    Semin Cancer Biol 20:329-39. 2010
  5. ncbi request reprint Functional role of the Werner syndrome RecQ helicase in human fibroblasts
    Kiranjit K Dhillon
    Department of Pathology, University of Washington, Seattle, WA 98195 7705, USA
    Aging Cell 6:53-61. 2007
  6. ncbi request reprint Design, activity, and structure of a highly specific artificial endonuclease
    Brett S Chevalier
    Fred Hutchinson Cancer Research Center and Graduate Program in Molecular and Cell Biology, University of Washington, 1100 Fairview Avenue N A3 023, Seattle, WA 98109, USA
    Mol Cell 10:895-905. 2002
  7. pmc Homologous recombination resolution defect in werner syndrome
    Yannick Saintigny
    Departments of Pathology Biostatistics Genome Sciences, University of Washington, Seattle, Washington 98195 7705, USA
    Mol Cell Biol 22:6971-8. 2002
  8. pmc Divergent cellular phenotypes of human and mouse cells lacking the Werner syndrome RecQ helicase
    Kiranjit K Dhillon
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    DNA Repair (Amst) 9:11-22. 2010
  9. pmc Microfluidic-assisted analysis of replicating DNA molecules
    Julia M Sidorova
    Department of Pathology, University of Washington, Seattle, Washington, USA
    Nat Protoc 4:849-61. 2009
  10. doi request reprint DNA polymerases and human disease
    Lawrence A Loeb
    Department of Pathology University of Washington, K 072 HSB, Box 357705, Seattle, Washington 98195 7705, USA
    Nat Rev Genet 9:594-604. 2008

Research Grants

Collaborators

Detail Information

Publications27

  1. ncbi request reprint Generation of highly site-specific DNA double-strand breaks in human cells by the homing endonucleases I-PpoI and I-CreI
    R J Monnat
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    Biochem Biophys Res Commun 255:88-93. 1999
    ..I-PpoI and I-CreI should be useful for analyzing DNA double-strand break repair in human cells and rDNA...
  2. pmc From broken to old: DNA damage, IGF1 endocrine suppression and aging
    Raymond J Monnat
    Department of Pathology, University of Washington, Box 357705 Seattle, WA 98195 7705, United States
    DNA Repair (Amst) 6:1386-90. 2007
    ..This endocrine signaling pathway is of particular interest as it is a key regulator of metabolism and longevity in many organisms...
  3. ncbi request reprint Werner syndrome protein--unwinding function to explain disease
    Raymond J Monnat
    Department of Pathology, University of Washington, Seattle, WA 98195, USA
    Sci Aging Knowledge Environ 2004:re3. 2004
    ..These new results provide clues to the origin of cell lineage-specific defects in WS patients and suggest a broader role for Werner protein function in determining disease risk in the general population...
  4. pmc Human RECQ helicases: roles in DNA metabolism, mutagenesis and cancer biology
    Raymond J Monnat
    Department of Pathology, University of Washington, Seattle, WA 98195 7705, USA
    Semin Cancer Biol 20:329-39. 2010
    ..I also discuss the emerging role for RECQ helicases as predictors of disease risk and the response to therapy...
  5. ncbi request reprint Functional role of the Werner syndrome RecQ helicase in human fibroblasts
    Kiranjit K Dhillon
    Department of Pathology, University of Washington, Seattle, WA 98195 7705, USA
    Aging Cell 6:53-61. 2007
    ....
  6. ncbi request reprint Design, activity, and structure of a highly specific artificial endonuclease
    Brett S Chevalier
    Fred Hutchinson Cancer Research Center and Graduate Program in Molecular and Cell Biology, University of Washington, 1100 Fairview Avenue N A3 023, Seattle, WA 98109, USA
    Mol Cell 10:895-905. 2002
    ..These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases...
  7. pmc Homologous recombination resolution defect in werner syndrome
    Yannick Saintigny
    Departments of Pathology Biostatistics Genome Sciences, University of Washington, Seattle, Washington 98195 7705, USA
    Mol Cell Biol 22:6971-8. 2002
    ....
  8. pmc Divergent cellular phenotypes of human and mouse cells lacking the Werner syndrome RecQ helicase
    Kiranjit K Dhillon
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    DNA Repair (Amst) 9:11-22. 2010
    ....
  9. pmc Microfluidic-assisted analysis of replicating DNA molecules
    Julia M Sidorova
    Department of Pathology, University of Washington, Seattle, Washington, USA
    Nat Protoc 4:849-61. 2009
    ..The simplicity and extensibility of this platform should facilitate DNA replication analyses using small samples from a variety of biological and clinical sources...
  10. doi request reprint DNA polymerases and human disease
    Lawrence A Loeb
    Department of Pathology University of Washington, K 072 HSB, Box 357705, Seattle, Washington 98195 7705, USA
    Nat Rev Genet 9:594-604. 2008
    ..We explore the reasons that might justify the need for so many DNA polymerases, describe their function and mode of regulation, and finally consider links between mutations in DNA polymerases and human disease...
  11. ncbi request reprint The RecQ helicase WRN is required for normal replication fork progression after DNA damage or replication fork arrest
    Julia M Sidorova
    Department of Pathology, University of Washington, Seattle, Washington 98195 7705, USA
    Cell Cycle 7:796-807. 2008
    ..The data provide a mechanistic basis for a better understanding of WRN-mediated maintenance of genomic stability and for predicting the outcomes of DNA-targeting chemotherapy in several adult cancers that silence WRN expression...
  12. ncbi request reprint Werner and Hutchinson-Gilford progeria syndromes: mechanistic basis of human progeroid diseases
    Brian A Kudlow
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Nat Rev Mol Cell Biol 8:394-404. 2007
    ....
  13. pmc Computational redesign of endonuclease DNA binding and cleavage specificity
    Justin Ashworth
    Howard Hughes Medical Institute and Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Nature 441:656-9. 2006
    ..These results suggest that computational protein design methods can have an important role in the creation of novel highly specific endonucleases for gene therapy and other applications...
  14. pmc Werner syndrome protein limits MYC-induced cellular senescence
    Carla Grandori
    Basic Sciences, Human Biology and Clinical Divisions, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 17:1569-74. 2003
    ..We propose that WRN up-regulation by MYC may promote MYC-driven tumorigenesis by preventing cellular senescence...
  15. ncbi request reprint The Werner syndrome protein has separable recombination and survival functions
    Cristina Swanson
    Department of Pathology, University of Washington, Box 357705, Seattle, WA 98195 7705, USA
    DNA Repair (Amst) 3:475-82. 2004
    ....
  16. pmc The human WRN and BLM RecQ helicases differentially regulate cell proliferation and survival after chemotherapeutic DNA damage
    Frances J Mao
    Department of Pathology, University of Washington, Seattle, Washington 98195 7705, USA
    Cancer Res 70:6548-55. 2010
    ..We also provide new information on functional redundancy between WRN and BLM. These results provide a strong rationale for further developing WRN and BLM as biomarkers of tumor chemotherapeutic responsiveness...
  17. pmc Distinct functions of human RECQ helicases WRN and BLM in replication fork recovery and progression after hydroxyurea-induced stalling
    Julia M Sidorova
    Department of Pathology, University of Washington, Seattle, WA 98195 7705, United States
    DNA Repair (Amst) 12:128-39. 2013
    ..Finally, we show that recovery from HU includes apparent removal of some of the DNA that was synthesized immediately after release from HU, a novel phenomenon that we refer to as nascent strand processing, NSP...
  18. pmc Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins
    Hui Li
    Department of Pathology, University of Washington, Box 357705, Seattle, WA 98195, USA
    Nucleic Acids Res 37:1650-62. 2009
    ....
  19. ncbi request reprint Metal-dependent DNA cleavage mechanism of the I-CreI LAGLIDADG homing endonuclease
    Brett Chevalier
    Graduate Program in Molecular and Cellular Biology, University of Washington and Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North A3 025, Seattle, Washington 98109, USA
    Biochemistry 43:14015-26. 2004
    ..Failure to occupy the shared metal site, as observed in the presence of calcium or when the metal-binding side chain from the LAGLIDADG motif (Asp 20) is mutated to asparagine, prevents cleavage by the enzyme...
  20. ncbi request reprint Mutability of an HNH nuclease imidazole general base and exchange of a deprotonation mechanism
    Jennifer H Eastberg
    Fred Hutchinson Cancer Research Center and Graduate Program in Molecular and Cellular Biology, University of Washington, 1100 Fairview Avenue North, A3 025, Seattle, Washington 98109, USA
    Biochemistry 46:7215-25. 2007
    ....
  21. pmc Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases
    Petra Volná
    Department of Pediatrics, University of Washington, Box 359300 CW, Seattle WA 98195, USA
    Nucleic Acids Res 35:2748-58. 2007
    ..Recapitulation of DNA binding and cleavage by surface-displayed LHEs provides a high-throughput approach to library screening that should facilitate rapid identification and analysis of enzymes with novel sequence specificities...
  22. pmc Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease
    Jennifer L Eklund
    Department of Genome Sciences, University of Washington, Seattle, WA, USA
    Nucleic Acids Res 35:5839-50. 2007
    ....
  23. pmc The unexpected landscape of in vivo somatic mutation in a human epithelial cell lineage
    Lorel M Colgin
    Department of Pathology, University of Washington, Box 357705, Seattle, WA 98195 7705, USA
    Proc Natl Acad Sci U S A 99:1437-42. 2002
    ....
  24. ncbi request reprint Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI
    Brett Chevalier
    Division of Basic Sciences, Graduate Program in Molecular and Cellular Biology, University of Washington and the Fred Hutchinson Cancer Research Center, Seattle 98109, USA
    J Mol Biol 329:253-69. 2003
    ....
  25. ncbi request reprint The Werner syndrome helicase is a cofactor for HIV-1 long terminal repeat transactivation and retroviral replication
    Anima Sharma
    Laboratory of Molecular Virology, Department of Biological Sciences, Southern Methodist University, Dallas, Texas 75275 0376, USA
    J Biol Chem 282:12048-57. 2007
    ..WRN may be a plausible target for antiretroviral therapy...
  26. ncbi request reprint Roles of ATM and NBS1 in chromatin structure modulation and DNA double-strand break repair
    Elijahu Berkovich
    Department of Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Nat Cell Biol 9:683-90. 2007
    ....
  27. doi request reprint Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites
    Elijahu Berkovich
    Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
    Nat Protoc 3:915-22. 2008
    ..Starting with fragmented chromatin, results can be achieved in 2-3 d...

Research Grants11

  1. NOVEL HUMAN GENE SPECIFIC REAGENTS FROM HOMING ENDOS
    Raymond Monnat; Fiscal Year: 2005
    ..The availability of human GSR's may also allow modification of altered human genes responsible for specific diseases or genes of pathogenic microorganisms including bacteria and viruses, parasites, etc. ..