Research Topics
| X MichaletSummaryAffiliation: University of California Country: USA Publications
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Publications
New light on quantum dot cytotoxicityJames M Tsay
Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA
Chem Biol 12:1159-61. 2005..In this issue of Chemistry & Biology, Lovrić and colleagues examine the likely role of reactive oxygen species in quantum dot cytotoxicity ...
Measuring diffusion with polarization-modulation dual-focus fluorescence correlation spectroscopyYou Korlann
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
Opt Express 16:14609-16. 2008..With pmFCS, the measured diffusion coefficient (D) for Atto655 maleimide in water at 25?C is determined to be equal to (4.09 +/- 0.07) x 10(-6)cm(2)/s, in good agreement with the value of 4.04 x 10-6cm2/s as measured by 2fFCS...- Mean square displacement analysis of single-particle trajectories with localization error: Brownian motion in an isotropic mediumXavier Michalet
Department of Chemistry and Biochemistry, University of California at Los Angeles, 607 Charles E Young Drive E, Los Angeles, California 90095, USA
Phys Rev E Stat Nonlin Soft Matter Phys 82:041914. 2010..We discuss the practical implications of these results for data analysis in single-particle tracking experiments... - Development of new photon-counting detectors for single-molecule fluorescence microscopyX Michalet
Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA 90095 1547, USA
Philos Trans R Soc Lond B Biol Sci 368:20120035. 2013..We also discuss our development of large area photon-counting cameras achieving subnanosecond resolution for fluorescence lifetime imaging applications at the single-molecule level... - Optimal diffusion coefficient estimation in single-particle trackingXavier Michalet
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095, USA
Phys Rev E Stat Nonlin Soft Matter Phys 85:061916. 2012..We discuss the practical performance of the algorithms as well as some important implications of these results for single-particle tracking... - Single-quantum dot imaging with a photon counting cameraX Michalet
Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA, USA
Curr Pharm Biotechnol 10:543-58. 2009..We illustrate the single-molecule imaging performance of our current prototype using quantum dots and discuss on-going and future developments of this detector... - Equilibrium shape degeneracy in starfish vesiclesXavier Michalet
Chemistry and Biochemistry Department, UCLA, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
Phys Rev E Stat Nonlin Soft Matter Phys 76:021914. 2007..These predictions are supported by experimental observations of a three-arm starfish vesicle undergoing spontaneous shape transformations similar to those predicted numerically... - The power and prospects of fluorescence microscopies and spectroscopiesXavier Michalet
Department of Chemistry and Biochemistry, UCLA, Young Hall, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
Annu Rev Biophys Biomol Struct 32:161-82. 2003.... - Single-molecule fluorescence studies of protein folding and conformational dynamicsXavier Michalet
Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Drive East, Los Angeles, California 90095, USA
Chem Rev 106:1785-813. 2006 - Using photon statistics to boost microscopy resolutionXavier Michalet
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 103:4797-8. 2006 - Quantum dots for live cells, in vivo imaging, and diagnosticsX Michalet
Department of Chemistry and Biochemistry, University of California, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
Science 307:538-44. 2005.... - Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitationNam Ki Lee
Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA
Biophys J 88:2939-53. 2005..ALEX can benefit structural analysis of biomolecules, especially when such molecules are inaccessible to conventional structural methods due to heterogeneity or transient nature... - Advances in fluorescence imaging with quantum dot bio-probesFabien Pinaud
Department of Chemistry and Biochemistry, UCLA, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
Biomaterials 27:1679-87. 2006.... - Lighting up individual DNA binding proteins with quantum dotsYuval Ebenstein
Department of Chemistry and Biochemistry, DOE Institute for Genomics and Proteomics, UCLA, Los Angeles, California 90095, USA
Nano Lett 9:1598-603. 2009..This work demonstrates the potential of this approach to precisely read protein binding position or, alternatively, "write" such information on extended DNA with QDs via sequence-specific molecular recognition... - High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cellsGopal Iyer
Department of Chemistry and Biochemistry, David Geffen School of Medicine, UCLA, Los Angeles, California 90024, USA
Nano Lett 8:4618-23. 2008..The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a... - Nanometer distance measurements between multicolor quantum dotsJosh Antelman
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angles, California 90095, USA
Nano Lett 9:2199-205. 2009..Our analysis opens the way to accurate structural studies of biomolecules and biomolecular complexes using multicolor quantum labeling... - Protein-protein interactions as a tool for site-specific labeling of proteinsMarcus Jager
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
Protein Sci 14:2059-68. 2005.... - Tracking single proteins in live cells using single-chain antibody fragment-fluorescent quantum dot affinity pairGopal Iyer
Department of Chemistry and Biochemistry, California NanoSystems Institute, University of California, Los Angeles, California, USA
Methods Enzymol 475:61-79. 2010.... - Shot-noise limited single-molecule FRET histograms: comparison between theory and experimentsEyal Nir
Department of Chemistry and Biochemistry, and California NanoScience Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
J Phys Chem B 110:22103-24. 2006..6 A. Finally, we briefly mention the results of a future publication and illustrate them with a simple two-state model system (DNA hairpin), for which the kinetic transition rates between the open and closed conformations are extracted... - Peptide coated quantum dots for biological applicationsGopal Iyer
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
IEEE Trans Nanobioscience 5:231-8. 2006.... - Dynamic partitioning of a glycosyl-phosphatidylinositol-anchored protein in glycosphingolipid-rich microdomains imaged by single-quantum dot trackingFabien Pinaud
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA, USA
Traffic 10:691-712. 2009.... - Femtomole mixer for microsecond kinetic studies of protein foldingDavid E Hertzog
Mechanical Engineering Department, Stanford University, Stanford, California 94305, USA
Anal Chem 76:7169-78. 2004..We also demonstrate the feasibility of measuring fast protein folding kinetics using FRET with acyl-CoA binding protein... - Periodic acceptor excitation spectroscopy of single moleculesSoren Doose
Clarendon Laboratory, Department of Physics and IRC in Bionanotechnology, Oxford University, Oxford, UK
Eur Biophys J 36:669-74. 2007....