X Michalet

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint New light on quantum dot cytotoxicity
    James M Tsay
    Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA
    Chem Biol 12:1159-61. 2005
  2. pmc Measuring diffusion with polarization-modulation dual-focus fluorescence correlation spectroscopy
    You Korlann
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Opt Express 16:14609-16. 2008
  3. pmc Mean square displacement analysis of single-particle trajectories with localization error: Brownian motion in an isotropic medium
    Xavier Michalet
    Department of Chemistry and Biochemistry, University of California at Los Angeles, 607 Charles E Young Drive E, Los Angeles, California 90095, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 82:041914. 2010
  4. pmc Development of new photon-counting detectors for single-molecule fluorescence microscopy
    X Michalet
    Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA 90095 1547, USA
    Philos Trans R Soc Lond B Biol Sci 368:20120035. 2013
  5. ncbi request reprint Optimal diffusion coefficient estimation in single-particle tracking
    Xavier Michalet
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 85:061916. 2012
  6. pmc Single-quantum dot imaging with a photon counting camera
    X Michalet
    Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA, USA
    Curr Pharm Biotechnol 10:543-58. 2009
  7. ncbi request reprint Equilibrium shape degeneracy in starfish vesicles
    Xavier Michalet
    Chemistry and Biochemistry Department, UCLA, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 76:021914. 2007
  8. ncbi request reprint The power and prospects of fluorescence microscopies and spectroscopies
    Xavier Michalet
    Department of Chemistry and Biochemistry, UCLA, Young Hall, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
    Annu Rev Biophys Biomol Struct 32:161-82. 2003
  9. pmc Single-molecule fluorescence studies of protein folding and conformational dynamics
    Xavier Michalet
    Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
    Chem Rev 106:1785-813. 2006
  10. pmc Using photon statistics to boost microscopy resolution
    Xavier Michalet
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 103:4797-8. 2006

Collaborators

Detail Information

Publications23

  1. ncbi request reprint New light on quantum dot cytotoxicity
    James M Tsay
    Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA
    Chem Biol 12:1159-61. 2005
    ..In this issue of Chemistry & Biology, Lovrić and colleagues examine the likely role of reactive oxygen species in quantum dot cytotoxicity ...
  2. pmc Measuring diffusion with polarization-modulation dual-focus fluorescence correlation spectroscopy
    You Korlann
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Opt Express 16:14609-16. 2008
    ..With pmFCS, the measured diffusion coefficient (D) for Atto655 maleimide in water at 25?C is determined to be equal to (4.09 +/- 0.07) x 10(-6)cm(2)/s, in good agreement with the value of 4.04 x 10-6cm2/s as measured by 2fFCS...
  3. pmc Mean square displacement analysis of single-particle trajectories with localization error: Brownian motion in an isotropic medium
    Xavier Michalet
    Department of Chemistry and Biochemistry, University of California at Los Angeles, 607 Charles E Young Drive E, Los Angeles, California 90095, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 82:041914. 2010
    ..We discuss the practical implications of these results for data analysis in single-particle tracking experiments...
  4. pmc Development of new photon-counting detectors for single-molecule fluorescence microscopy
    X Michalet
    Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA 90095 1547, USA
    Philos Trans R Soc Lond B Biol Sci 368:20120035. 2013
    ..We also discuss our development of large area photon-counting cameras achieving subnanosecond resolution for fluorescence lifetime imaging applications at the single-molecule level...
  5. ncbi request reprint Optimal diffusion coefficient estimation in single-particle tracking
    Xavier Michalet
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 85:061916. 2012
    ..We discuss the practical performance of the algorithms as well as some important implications of these results for single-particle tracking...
  6. pmc Single-quantum dot imaging with a photon counting camera
    X Michalet
    Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA, USA
    Curr Pharm Biotechnol 10:543-58. 2009
    ..We illustrate the single-molecule imaging performance of our current prototype using quantum dots and discuss on-going and future developments of this detector...
  7. ncbi request reprint Equilibrium shape degeneracy in starfish vesicles
    Xavier Michalet
    Chemistry and Biochemistry Department, UCLA, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 76:021914. 2007
    ..These predictions are supported by experimental observations of a three-arm starfish vesicle undergoing spontaneous shape transformations similar to those predicted numerically...
  8. ncbi request reprint The power and prospects of fluorescence microscopies and spectroscopies
    Xavier Michalet
    Department of Chemistry and Biochemistry, UCLA, Young Hall, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
    Annu Rev Biophys Biomol Struct 32:161-82. 2003
    ....
  9. pmc Single-molecule fluorescence studies of protein folding and conformational dynamics
    Xavier Michalet
    Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
    Chem Rev 106:1785-813. 2006
  10. pmc Using photon statistics to boost microscopy resolution
    Xavier Michalet
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 103:4797-8. 2006
  11. pmc Quantum dots for live cells, in vivo imaging, and diagnostics
    X Michalet
    Department of Chemistry and Biochemistry, University of California, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
    Science 307:538-44. 2005
    ....
  12. pmc Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation
    Nam Ki Lee
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 1569, USA
    Biophys J 88:2939-53. 2005
    ..ALEX can benefit structural analysis of biomolecules, especially when such molecules are inaccessible to conventional structural methods due to heterogeneity or transient nature...
  13. pmc Advances in fluorescence imaging with quantum dot bio-probes
    Fabien Pinaud
    Department of Chemistry and Biochemistry, UCLA, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
    Biomaterials 27:1679-87. 2006
    ....
  14. pmc Lighting up individual DNA binding proteins with quantum dots
    Yuval Ebenstein
    Department of Chemistry and Biochemistry, DOE Institute for Genomics and Proteomics, UCLA, Los Angeles, California 90095, USA
    Nano Lett 9:1598-603. 2009
    ..This work demonstrates the potential of this approach to precisely read protein binding position or, alternatively, "write" such information on extended DNA with QDs via sequence-specific molecular recognition...
  15. pmc High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells
    Gopal Iyer
    Department of Chemistry and Biochemistry, David Geffen School of Medicine, UCLA, Los Angeles, California 90024, USA
    Nano Lett 8:4618-23. 2008
    ..The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a...
  16. pmc Nanometer distance measurements between multicolor quantum dots
    Josh Antelman
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angles, California 90095, USA
    Nano Lett 9:2199-205. 2009
    ..Our analysis opens the way to accurate structural studies of biomolecules and biomolecular complexes using multicolor quantum labeling...
  17. pmc Protein-protein interactions as a tool for site-specific labeling of proteins
    Marcus Jager
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Protein Sci 14:2059-68. 2005
    ....
  18. pmc Tracking single proteins in live cells using single-chain antibody fragment-fluorescent quantum dot affinity pair
    Gopal Iyer
    Department of Chemistry and Biochemistry, California NanoSystems Institute, University of California, Los Angeles, California, USA
    Methods Enzymol 475:61-79. 2010
    ....
  19. pmc Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments
    Eyal Nir
    Department of Chemistry and Biochemistry, and California NanoScience Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Phys Chem B 110:22103-24. 2006
    ..6 A. Finally, we briefly mention the results of a future publication and illustrate them with a simple two-state model system (DNA hairpin), for which the kinetic transition rates between the open and closed conformations are extracted...
  20. pmc Peptide coated quantum dots for biological applications
    Gopal Iyer
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
    IEEE Trans Nanobioscience 5:231-8. 2006
    ....
  21. pmc Dynamic partitioning of a glycosyl-phosphatidylinositol-anchored protein in glycosphingolipid-rich microdomains imaged by single-quantum dot tracking
    Fabien Pinaud
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA, USA
    Traffic 10:691-712. 2009
    ....
  22. pmc Femtomole mixer for microsecond kinetic studies of protein folding
    David E Hertzog
    Mechanical Engineering Department, Stanford University, Stanford, California 94305, USA
    Anal Chem 76:7169-78. 2004
    ..We also demonstrate the feasibility of measuring fast protein folding kinetics using FRET with acyl-CoA binding protein...
  23. pmc Periodic acceptor excitation spectroscopy of single molecules
    Soren Doose
    Clarendon Laboratory, Department of Physics and IRC in Bionanotechnology, Oxford University, Oxford, UK
    Eur Biophys J 36:669-74. 2007
    ....