Research Topics
Genomes and Genes | JOSEPH MIANOSummaryAffiliation: University of Rochester Country: USA Publications
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Publications
Serum response factor: master regulator of the actin cytoskeleton and contractile apparatusJoseph M Miano
Cardiovascular Research Institute, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642, USA
Am J Physiol Cell Physiol 292:C70-81. 2007..This review summarizes the recent genomic and genetic analyses of CArG-SRF that support its role as an ancient, master regulator of the actin cytoskeleton and contractile machinery...- Serum response factor: toggling between disparate programs of gene expressionJoseph M Miano
Center for Cardiovascular Research, University of Rochester Medical Center, 601 Elmwood Avenue, NY 14642, Rochester, USA
J Mol Cell Cardiol 35:577-93. 2003..The purpose of this review is to summarize the history and progress made in SRF research with emphasis on the role this transcription factor plays in facilitating a program of SMC-restricted gene expression... 
Role of serum response factor in the pathogenesis of diseaseJoseph M Miano
Department of Medicine, Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
Lab Invest 90:1274-84. 2010..This review summarizes data from experimental model systems and human pathology where SRF expression is either deliberately or naturally altered...
Dicing up microRNA gene expression profiles in normal and neoplastic smooth muscle cellsJoseph M Miano
Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
Am J Pathol 177:541-3. 2010..This Commentary discusses the role of miRNA expression profiles in identifying malignancy...- Restricted inactivation of serum response factor to the cardiovascular systemJoseph M Miano
Center for Cardiovascular Research, Aab Institute of Biomedical Sciences, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
Proc Natl Acad Sci U S A 101:17132-7. 2004..These results suggest a vital role for SRF in contractile/cytoskeletal architecture necessary for the proper assembly and function of cardiomyocytes and vascular SMC... - The smooth muscle cell-restricted KCNMB1 ion channel subunit is a direct transcriptional target of serum response factor and myocardinXiaochun Long
Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
J Biol Chem 284:33671-82. 2009..These results reveal the first ion channel subunit as a direct target of SRF-MYOCD transactivation, providing further insight into the role of MYOCD as a master regulator of the SMC contractile phenotype... - AKAP12alpha, an atypical serum response factor-dependent target geneJeffrey W Streb
Center for Cardiovascular Research in the Aab Institute of Biomedical Sciences, University of Rochester School of Medicine, Rochester, New York 14642, USA
J Biol Chem 280:4125-34. 2005..Furthermore, we provide new insight into the role of flanking sequences in determining sensitivity to SRF-myocardin activity... - Expression and functional activity of four myocardin isoformsMasaaki Imamura
Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, New York 14642, USA
Gene 464:1-10. 2010..We propose a new myocardin nomenclature reflecting the dominant splice variants expressed in cardiac muscle (Myocd_v1 and v2) versus SMC-rich tissues (Myocd_v3 and v4)... - Remote control of gene expressionXiaochun Long
Cardiovascular Research Institute, University of Rochester School of Medicine, Rochester, New York 14642, USA
J Biol Chem 282:15941-5. 2007..This review summarizes current approaches to characterize remote control of gene expression in transgenic mice and inherent limitations for accurately interpreting the essential nature of CRM activity... - Serum response factor and myocardin mediate arterial hypercontractility and cerebral blood flow dysregulation in Alzheimer's phenotypeNienwen Chow
Socratech Research Laboratories L L C, Department of Neurosurgery, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642, USA
Proc Natl Acad Sci U S A 104:823-8. 2007..Thus, SRF-MYOCD overexpression in small cerebral arteries appears to initiate independently of Abeta a pathogenic pathway mediating arterial hypercontractility and CBF dysregulation, which are associated with Alzheimer's dementia... - Neurovascular pathways and Alzheimer amyloid beta-peptideBerislav V Zlokovic
Frank R Smith Laboratories for Neuroscience and Neurosurgical Research, University of Rochester Medical Center, Rochester, NY 14642, USA
Brain Pathol 15:78-83. 2005..We suggest that there is a link between neurovascular dysfunction and elevated brain Abeta which provides a new scenario for therapeutic interventions to control Alzheimer mental deterioration... - Expression of human smooth muscle calponin in transgenic mice revealed with a bacterial artificial chromosomeJoseph M Miano
Center for Cardiovascular Research, University of Rochester Medical Center, Rochester, New York 14642, USA
Am J Physiol Heart Circ Physiol 282:H1793-803. 2002..These BAC mice represent a new model system in which to systematically assess regulatory elements governing SM-Calp transcription in vivo... - SRF and myocardin regulate LRP-mediated amyloid-beta clearance in brain vascular cellsRobert D Bell
Center for Neurodegenerative and Vascular Brain Disorders, Arthur Kornberg Medical Research Building, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, New York 14642, USA
Nat Cell Biol 11:143-53. 2009..Hypoxia stimulated SRF/MYOCD expression in human cerebral VSMCs and in animal models of AD. We suggest that SRF and MYOCD function as a transcriptional switch, controlling Abeta cerebrovascular clearance and progression of AD... - Myocardin-dependent activation of the CArG box-rich smooth muscle gamma-actin gene: preferential utilization of a single CArG element through functional association with the NKX3.1 homeodomain proteinQiang Sun
Aab Cardiovascular Research Institute of the University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
J Biol Chem 284:32582-90. 2009..These results demonstrate the ability of MYOCD to discriminate among several juxtaposed CArG elements, presumably through its novel partnership with NKX3.1, to optimally transactivate the human ACTG2 promoter... - Myocardin: a component of a molecular switch for smooth muscle differentiationJiyuan Chen
Center for Cardiovascular Research, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
J Mol Cell Cardiol 34:1345-56. 2002..Results reveal the expression of several SMC markers concomitant with a lower growth potential. Collectively, these studies suggest that myocardin is an important component of a molecular switch for the SMC differentiation program... - Expression and comparative genomics of two serum response factor genes in zebrafishJody L Davis
Department of Biological Sciences, State University of New York College at Brockport, Brockport, NY, USA
Int J Dev Biol 52:389-96. 2008.... - Defining the mammalian CArGomeQiang Sun
Cardiovascular Research Institute, University of Rochester School of Medicine, Rochester, NY 14642, USA
Genome Res 16:197-207. 2006..We suggest one function of SRF is that of an ancient master regulator of the actin cytoskeleton... - Myocardin is a bifunctional switch for smooth versus skeletal muscle differentiationXiaochun Long
The Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
Proc Natl Acad Sci U S A 104:16570-5. 2007.... - Mammalian smooth muscle differentiation: origins, markers and transcriptional controlJoseph M Miano
Center for Cardiovascular Research, Box 679, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, New York 14642, USA
Results Probl Cell Differ 38:39-59. 2002 - Myocardin is sufficient for a smooth muscle-like contractile phenotypeXiaochun Long
Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, 211 Bailey Road, Rochester, New York 14586, USA
Arterioscler Thromb Vasc Biol 28:1505-10. 2008..Here, we sought to ascertain whether Myocd-mediated gene expression confers a structural and physiological cardiac or SMC phenotype... - Tissue expression of the novel serine carboxypeptidase Scpep1Ting Hein D Lee
Cardiovascular Research Institute, University of Rochester School of Medicine, 601 Elmwood Avenue, Box 679, Rochester, NY 14642, USA
J Histochem Cytochem 54:701-11. 2006..The highly specific Scpep1 antibody characterized herein provides a necessary reagent for elucidating Scpep1 function... - Functional significance of protein kinase A activation by endothelin-1 and ATP: negative regulation of SRF-dependent gene expression by PKAAmanda Davis
Section of Pulmonary and Critical Care Medicine, Department of Medicine, The University of Chicago, 5841 S. Maryland Avenue, MC 6076, Chicago, IL 60637, USA
Cell Signal 15:597-604. 2003..These data demonstrate that (i) PKA negatively regulates SRF activity and (ii) ET1 and ATP stimulate opposing pathways, whose balance determines the net activity of SRF... - Role of phosphodiesterase 3 in NO/cGMP-mediated antiinflammatory effects in vascular smooth muscle cellsToru Aizawa
University of Rochester, Center for Cardiovascular Research, 601 Elmwood Ave, Box 679, Rochester, NY 14642, USA
Circ Res 93:406-13. 2003..These results suggest that SNAP and CNP exert inhibitory effects on NF-kappaB-dependent transcription by activation of PKA via cGMP-dependent inhibition of PDE3 activity. Therefore, PDE3 is a novel mediator of inflammation in VSMCs... - Multiple promoters direct expression of three AKAP12 isoforms with distinct subcellular and tissue distribution profilesJeffrey W Streb
Center for Cardiovascular Research in the Aab Institute of Biomedical Sciences, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
J Biol Chem 279:56014-23. 2004..Our results demonstrate heretofore unrecognized complexity within the AKAP12 locus and suggest a mechanism for genetic control of signaling specificity through distinct regulation of alternately targeted anchoring protein isoforms... - Smooth muscle calponin: an unconventional CArG-dependent gene that antagonizes neointimal formationXiaochun Long
Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, NY 14642, USA
Arterioscler Thromb Vasc Biol 31:2172-80. 2011..The objectives here were to evaluate these CArG elements for activity in transgenic mice and determine the effect of human CNN1 on injury-induced vascular remodeling... - Tumor suppressor MDA-7/IL-24 selectively inhibits vascular smooth muscle cell growth and migrationJiyuan Chen
Center for Cardiovascular Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 679, Rochester, New York 14642, USA
Mol Ther 8:220-9. 2003..These data demonstrate that Ad-mda7 exhibits selectivity in apoptosis induction and growth suppression in an atypical SMC line, raising new questions pertaining to heterogeneity in SMC death susceptibility... - Functional characterization of a putative serine carboxypeptidase in vascular smooth muscle cellsTing Hein Lee
Aab Cardiovascular Research Institute, Box CVRI, University of Rochester School of Medicine and Dentistry, 211 Bailey Rd, Rochester, New York 14586, USA
Circ Res 105:271-8. 2009..SCPEP1 bears the signature catalytic triad found in all serine carboxypeptidases, but its biological function is completely unknown... - Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in vascular smooth muscle cell growth and survivalDavid J Nagel
Cardiovascular Research Institute, University of Rochester, New York, USA
Circ Res 98:777-84. 2006..These suggest that nuclear PDE1A is important in VSMC growth and survival and may contribute to the neointima formation in atherosclerosis and restenosis... - Cross-species sequence analysis reveals multiple charged residue-rich domains that regulate nuclear/cytoplasmic partitioning and membrane localization of a kinase anchoring protein 12 (SSeCKS/Gravin)Jeffrey W Streb
Center for Cardiovascular Research in the Aab Institute of Biomedical Sciences, University of Rochester School of Medicine, Rochester, New York 14642, USA
J Biol Chem 280:28007-14. 2005..Our data suggest that AKAP12 localization is regulated by a hierarchy of targeting domains and that the localization of AKAP12-assembled signaling complexes may be dynamically regulated... - SRF'ing the actin cytoskeleton with no destrinJoseph M Miano
Physiol Genomics 34:6-8. 2008 - Cardiac-specific gene expression: a HANDful of factorsJoseph M Miano
J Mol Cell Cardiol 34:1287-91. 2002 - Contribution of serum response factor and myocardin to transcriptional regulation of smoothelinsSander S M Rensen
Department of Genetics and Cell Biology, Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands
Cardiovasc Res 70:136-45. 2006..Our data show a role for SRF/myocardin in regulating smoothelin-A whereas the higher smoothelin-B expression appears to be SRF/myocardin-independent... - Smooth muscle--specific expression of CYP4A1 induces endothelial sprouting in renal arterial microvesselsMiao Jiang
Department of Pharmacology, New York Medical College, Valhalla, NY 10595, USA
Circ Res 94:167-74. 2004..We conclude that Ad-SM22-4A1 drives a smooth muscle-specific functional expression of CYP4A1 and demonstrates increased angiogenesis, presumably via increased production of 20-HETE... - Myocardin enhances Smad3-mediated transforming growth factor-beta1 signaling in a CArG box-independent manner: Smad-binding element is an important cis element for SM22alpha transcription in vivoPing Qiu
Department of Internal Medicine, Wayne State University, Detroit, Michigan, USA
Circ Res 97:983-91. 2005..This is the first demonstration that Myocd can act as a transcriptional coactivator of the smooth muscle regulatory network in a CArG box-independent manner... - Channeling to myocardinJoseph M Miano
Circ Res 95:340-2. 2004 - Dual role of PKA in phenotypic modulation of vascular smooth muscle cells by extracellular ATPD Kyle Hogarth
Section of Pulmonary and Critical Care Medicine, Dept. of Medicine, University of Chicago, 5841 S. Maryland Avenue, MC 6076, Chicago, IL 60637, USA
Am J Physiol Cell Physiol 287:C449-56. 2004..Transformation of VSMC by high doses of ATP can be prevented and even reversed by inhibition of PKA activity... - HDAC7 supports vascular integrityJoseph M Miano
Nat Med 12:997-8. 2006 - Deck of CArGsJoseph M Miano
Circ Res 103:13-5. 2008 - Fishing for function in zebrafishJoseph M Miano
Circ Res 98:723-6. 2006 - Angiotensin II: a devious activator of mineralocorticoid receptor-dependent gene expressionBurns C Blaxall
Circ Res 96:610-1. 2005
Research Grants
- SRF/MYOCD: new targets in Alzheimer's neurovasculatureJOSEPH MIANO; Fiscal Year: 2006....
- Molecular Control of Smooth Muscle Calponin ExpressionJOSEPH MIANO; Fiscal Year: 2007..This research proposal seeks to gain a deep understanding of how one such gene, called CNN1, is regulated under normal and disease-associated circumstances. ..
- Molecular Control of Smooth Muscle Calponin ExpressionJoseph M Miano; Fiscal Year: 2010..This research proposal seeks to gain a deep understanding of how one such gene, called CNN1, is regulated under normal and disease-associated circumstances. ..
- Analysis of a Retinoid-Induced Tumor Suppressor in SMCJOSEPH MIANO; Fiscal Year: 2006..Such information has enormous potential for understanding and treating human vascular Idisease and other pathological disorders, including cancer where retinoids have been in clinical use for years. ..
- Molecular Control of Smooth Muscle Calponin ExpressionJOSEPH MIANO; Fiscal Year: 2006..Such information has enormous value for understanding and potentially treating vascular diseases as well as pathological vessel development. ..
- MOLECULAR CONTROL--SMOOTH MUSCLE CALPONIN EXPRESSIONJOSEPH MIANO; Fiscal Year: 2002..Such information will facilitate the design and implementation of potentially novel molecular interventions for the treatment of vascular disease. ..
- Bifunctionality of Myocardin in MyogenesisJoseph M Miano; Fiscal Year: 2010..This information has direct applications to a wide variety of diseases including those of the heart and blood vessels, Alzheimer's disease, and asthma. ..