William W Metcalf
Affiliation: University of Illinois
Fu H, Goettge M, Metcalf W. Biochemical characterization of the methylmercaptopropionate:cob(I)alamin methyltransferase from Methanosarcina acetivorans. J Bacteriol. 2019;: pubmed publisher
..However, the enzyme does not catalyze a proposed second step required to produce the key intermediate methyl-CoM. Therefore, additional enzymes required for methanogenic MMPA catabolism await discovery. ..
LÃ³pez MuÃ±oz M, SchÃ¶nheit P, Metcalf W. Genetic, Genomic, and Transcriptomic Studies of Pyruvate Metabolism in Methanosarcina barkeri Fusaro. J Bacteriol. 2015;197:3592-600 pubmed publisher
..These results clarify the mechanism of methanogenic catabolism of pyruvate and expand our understanding of carbon assimilation in methanogens. ..
Goettge M, Cioni J, Ju K, Pallitsch K, Metcalf W. PcxL and HpxL are flavin-dependent, oxime-forming N-oxidases in phosphonocystoximic acid biosynthesis in Streptomyces. J Biol Chem. 2018;293:6859-6868 pubmed publisher
..Bioinformatic analysis indicated that PcxL and HpxL are members of a new class of oxime-forming N-oxidases that are broadly dispersed among bacteria. ..
Kulkarni G, Mand T, Metcalf W. Energy Conservation via Hydrogen Cycling in the Methanogenic Archaeon Methanosarcina barkeri. MBio. 2018;9: pubmed publisher
..Experimental validation of H2 cycling adds to a short list of mechanisms for generating a transmembrane electrochemical gradient that is likely to be widespread, especially among anaerobic microorganisms. ..
Fu H, Metcalf W. Genetic basis for metabolism of methylated sulfur compounds in Methanosarcina species. J Bacteriol. 2015;197:1515-24 pubmed publisher
..These data will aid the development of predictive sulfur cycle models and enable molecular ecological approaches for the study of methylated sulfur metabolism in anaerobic ecosystems. ..
Nayak D, Metcalf W. Cas9-mediated genome editing in the methanogenic archaeon Methanosarcina acetivorans. Proc Natl Acad Sci U S A. 2017;114:2976-2981 pubmed publisher