WILLIAM METCALF

Summary

Affiliation: University of Illinois
Country: USA

Publications

  1. pmc Synthesis of methylphosphonic acid by marine microbes: a source for methane in the aerobic ocean
    William W Metcalf
    Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, USA
    Science 337:1104-7. 2012
  2. pmc Biosynthesis of phosphonic and phosphinic acid natural products
    William W Metcalf
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Annu Rev Biochem 78:65-94. 2009
  3. pmc An anaerobic, intrachamber incubator for growth of Methanosarcina spp. on methanol-containing solid media
    W W Metcalf
    Department of Microbiology, University of Illinois, Urbana 61801, USA
    Appl Environ Microbiol 64:768-70. 1998
  4. pmc Molecular genetic analysis of phosphite and hypophosphite oxidation by Pseudomonas stutzeri WM88
    W W Metcalf
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Bacteriol 180:5547-58. 1998
  5. ncbi request reprint Unusual transformations in the biosynthesis of the antibiotic phosphinothricin tripeptide
    Joshua A V Blodgett
    Department of Microbiology, University of Illinois at Urbana Champaign, B103 CLSL, 601 S Goodwin, Urbana, Illinois 61801, USA
    Nat Chem Biol 3:480-5. 2007
  6. pmc Characterization and structure of DhpI, a phosphonate O-methyltransferase involved in dehydrophos biosynthesis
    Jin Hee Lee
    Department of Chemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 107:17557-62. 2010
  7. pmc Molecular cloning and heterologous expression of the dehydrophos biosynthetic gene cluster
    Benjamin T Circello
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Chem Biol 17:402-11. 2010
  8. pmc Biosynthesis of rhizocticins, antifungal phosphonate oligopeptides produced by Bacillus subtilis ATCC6633
    Svetlana A Borisova
    Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Chem Biol 17:28-37. 2010
  9. pmc An unusual carbon-carbon bond cleavage reaction during phosphinothricin biosynthesis
    Robert M Cicchillo
    Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Nature 459:871-4. 2009
  10. pmc Biosynthesis of 2-hydroxyethylphosphonate, an unexpected intermediate common to multiple phosphonate biosynthetic pathways
    Zengyi Shao
    Department of Chemical and Biomolecular Engineering and Chemistry, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Biol Chem 283:23161-8. 2008

Research Grants

Collaborators

Detail Information

Publications12

  1. pmc Synthesis of methylphosphonic acid by marine microbes: a source for methane in the aerobic ocean
    William W Metcalf
    Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, USA
    Science 337:1104-7. 2012
    ..The abundance of a key gene in this pathway in metagenomic data sets suggests that methylphosphonate biosynthesis is relatively common in marine microbes, providing a plausible explanation for the methane paradox...
  2. pmc Biosynthesis of phosphonic and phosphinic acid natural products
    William W Metcalf
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Annu Rev Biochem 78:65-94. 2009
    ..These investigations have also suggested new strategies for natural product discovery...
  3. pmc An anaerobic, intrachamber incubator for growth of Methanosarcina spp. on methanol-containing solid media
    W W Metcalf
    Department of Microbiology, University of Illinois, Urbana 61801, USA
    Appl Environ Microbiol 64:768-70. 1998
    ..In one operation, it is possible to incubate 153 petri plates, the equivalent of 11 standard anaerobic jars, with plating efficiencies identical to those of traditional protocols...
  4. pmc Molecular genetic analysis of phosphite and hypophosphite oxidation by Pseudomonas stutzeri WM88
    W W Metcalf
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Bacteriol 180:5547-58. 1998
    ..The finding of genes dedicated to oxidation of reduced P compounds provides further evidence that a redox cycle for P may be important in the metabolism of this essential, and often growth-limiting, nutrient...
  5. ncbi request reprint Unusual transformations in the biosynthesis of the antibiotic phosphinothricin tripeptide
    Joshua A V Blodgett
    Department of Microbiology, University of Illinois at Urbana Champaign, B103 CLSL, 601 S Goodwin, Urbana, Illinois 61801, USA
    Nat Chem Biol 3:480-5. 2007
    ..In sum, we define a pathway for early PTT biosynthesis that is more complex than previously appreciated...
  6. pmc Characterization and structure of DhpI, a phosphonate O-methyltransferase involved in dehydrophos biosynthesis
    Jin Hee Lee
    Department of Chemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 107:17557-62. 2010
    ..Interestingly, the enzyme has low substrate specificity and was used to methylate the clinical antibiotic fosfomycin and the antimalaria clinical candidate fosmidomycin, showing its promise for applications in bioengineering...
  7. pmc Molecular cloning and heterologous expression of the dehydrophos biosynthetic gene cluster
    Benjamin T Circello
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Chem Biol 17:402-11. 2010
    ..The early steps of this pathway are supported by analysis of intermediates accumulated by blocked mutants and in vitro biochemical experiments...
  8. pmc Biosynthesis of rhizocticins, antifungal phosphonate oligopeptides produced by Bacillus subtilis ATCC6633
    Svetlana A Borisova
    Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Chem Biol 17:28-37. 2010
    ..Recombinant RhiG was prepared, and the product of an in vitro enzymatic conversion was characterized. Access to this intermediate allows for biochemical characterization of subsequent steps in the pathway...
  9. pmc An unusual carbon-carbon bond cleavage reaction during phosphinothricin biosynthesis
    Robert M Cicchillo
    Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Nature 459:871-4. 2009
    ..The current study expands the scope of reactions catalysed by the 2-His-1-carboxylate mononuclear non-haem iron family of enzymes...
  10. pmc Biosynthesis of 2-hydroxyethylphosphonate, an unexpected intermediate common to multiple phosphonate biosynthetic pathways
    Zengyi Shao
    Department of Chemical and Biomolecular Engineering and Chemistry, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Biol Chem 283:23161-8. 2008
    ....
  11. ncbi request reprint Reassignment of the structure of the antibiotic A53868 reveals an unusual amino dehydrophosphonic acid
    John T Whitteck
    Department of Chemistry, University of Illinois at Urbana Champaign, 600 S Mathews Ave, Urbana, IL 61801, USA
    Angew Chem Int Ed Engl 46:9089-92. 2007
  12. pmc Two C-P lyase operons in Pseudomonas stutzeri and their roles in the oxidation of phosphonates, phosphite, and hypophosphite
    Andrea K White
    Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601 S Goodwin Ave, Urbana, IL 61801, USA
    J Bacteriol 186:4730-9. 2004
    ..The substrate ranges of both C-P lyases are limited, as growth on other phosphonate compounds, including glyphosate and phenylphosphonate, was not observed...

Research Grants10

  1. Discovery, Design, and Development of Phosphonic Acid Antibiotics
    WILLIAM METCALF; Fiscal Year: 2007
    ..The project will be housed in the new Institute for Genomic Biology at the University of Illinois, where the program project team will occupy a single, large contiguous laboratory. ..
  2. MICROBIAL METABOLISM OF REDUCED PHOSPHORUS COMPOUNDS
    WILLIAM METCALF; Fiscal Year: 2007
    ....
  3. MICROBIAL METABOLISMS OF REDUCED PHOSPHOROUS COMPOUNDS
    WILLIAM METCALF; Fiscal Year: 2004
    ..abstract_text> ..