Research Topics
| Craig MelloSummaryAffiliation: University of Massachusetts Medical School Country: USA Publications
Research Grants
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Detail Information
Publications
Revealing the world of RNA interferenceCraig C Mello
Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nature 431:338-42. 2004..At the heart of RNA interference lies a remarkable RNA processing mechanism that is now known to underlie many distinct biological phenomena...
Return to the RNAi world: rethinking gene expression and evolution (Nobel Lecture)Craig C Mello
Howard Hughes Medical Institute and Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
Angew Chem Int Ed Engl 46:6985-94. 2007- Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germlineWeifeng Gu
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA
Mol Cell 36:231-44. 2009..These findings broaden our understanding of the biogenesis and diversity of 22G-RNAs and suggest additional regulatory functions for small RNAs... - The Conserved Kinases CDK-1, GSK-3, KIN-19, and MBK-2 Promote OMA-1 Destruction to Regulate the Oocyte-to-Embryo Transition in C. elegansMasaki Shirayama
Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, Massachusetts 01605
Curr Biol 16:47-55. 2006..The asymmetric localization of these and other determinants is regulated in early embryos through motor-dependent physical translocation as well as selective proteolysis... - Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathwaysThomas F Duchaine
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01605, USA
Cell 124:343-54. 2006..Our findings provide a first glimpse at the complex biochemical niche of Dicer and suggest that competition exists between DCR-1-mediated small-RNA pathways... - SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C. elegans embryosYanxia Bei
Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
Dev Cell 3:113-25. 2002..Our findings suggest that Wnt and Src signaling function in parallel to control developmental outcomes within a single responding cell... - NMY-2 maintains cellular asymmetry and cell boundaries, and promotes a SRC-dependent asymmetric cell divisionJi Liu
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Dev Biol 339:366-73. 2010..Finally, during a signaling-induced asymmetric cell division, NMY-2 is required for SRC-dependent phosphotyrosine signaling and acts in parallel with WNT-signaling to specify endoderm... - Sequence-specific inhibition of small RNA functionGyorgy Hutvagner
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
PLoS Biol 2:E98. 2004.... - Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAiErbay Yigit
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 127:747-57. 2006..Interestingly, these AGO proteins lack key residues required for mRNA cleavage. Our findings support a two-step model for RNAi, in which functionally and structurally distinct AGOs act sequentially to direct gene silencing... - RNA interference: big applause for silencing in StockholmPhillip D Zamore
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 127:1083-6. 2006Eight years ago, Craig Mello, Andrew Fire, and their coworkers provided the first demonstration that double-stranded RNA (dsRNA) triggers the gene-silencing technique that we now call RNA interference (RNAi)... - RNA interference in Caenorhabditis elegansDarryl Conte
University of Massachusetts Medical School, Worcester, Massachusetts, USA
Curr Protoc Mol Biol . 2003..RNAi has become an essential tool in C. elegans research. Procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA and soaking in dsRNA solution are described in this unit... - Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryosKuniaki Nakamura
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 19:1749-54. 2005..We show that both PRY-1/Axin and the nuclear exportin homolog IMB-4/CRM-1 antagonize signaling. These findings reveal how Wnt signals direct the asymmetric localization of beta-catenin during polarized cell division... - A member of the polymerase beta nucleotidyltransferase superfamily is required for RNA interference in C. elegansChun Chieh G Chen
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Curr Biol 15:378-83. 2005..These findings implicate a new enzymatic modality in RNAi and suggest possible models for the role of RDE-3 in the RNAi mechanism... - The Caenorhabditis elegans IMPAS gene, imp-2, is essential for development and is functionally distinct from related presenilinsAnastasia P Grigorenko
Department of Psychiatry, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, 303 Belmont Street, Worcester, MA 01604, USA
Proc Natl Acad Sci U S A 101:14955-60. 2004..These data suggest that IMPs are functionally distinct from related PSs and implicate IMPs as critical regulators of development that may potentially interact with the lipid-lipoprotein receptor-mediated pathway... - Sequential rounds of RNA-dependent RNA transcription drive endogenous small-RNA biogenesis in the ERGO-1/Argonaute pathwayJessica J Vasale
Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 107:3582-7. 2010..ERGO-1 targets exhibit a nonrandom distribution in the genome and appear to include many gene duplications, suggesting that this pathway may control overexpression resulting from gene expansion... - The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegansHiroaki Tabara
Program in Molecular Medicine, University of Massachusetts Meidcal School, Worcester, MA 1605, USA
Cell 109:861-71. 2002..Our findings suggest a model in which RDE-4 and RDE-1 function together to detect and retain foreign dsRNA and to present this dsRNA to DCR-1 for processing... - RNAi (Nematodes: Caenorhabditis elegans)Alla Grishok
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester 01605, USA
Adv Genet 46:339-60. 2002..We discuss RNAi-deficient mutants in C. elegans as well as characterized components of the RNAi, pathway, the molecular mechanism of RNAi, and its possible role in development and immunity... - The minibrain kinase homolog, mbk-2, is required for spindle positioning and asymmetric cell division in early C. elegans embryosKa Ming Pang
Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Dev Biol 265:127-39. 2004..These findings in conjunction with work from Schizosaccharomyces pombe indicate a possible conserved role for Dyrk family kinases in the regulation of spindle placement during cell division... - MEP-1 and a homolog of the NURD complex component Mi-2 act together to maintain germline-soma distinctions in C. elegansYingdee Unhavaithaya
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 111:991-1002. 2002.... - The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregationJulie M Claycomb
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA
Cell 139:123-34. 2009..Instead, our findings support a model in which CSR-1 complexes target protein-coding domains to promote their proper organization within the holocentric chromosomes of C. elegans... - Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegansColin C Conine
The Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01606, USA
Proc Natl Acad Sci U S A 107:3588-93. 2010..These findings add to a growing number of AGO pathways required for thermotolerant fertility in C. elegans and support a model in which AGOs and their small RNA cofactors function to promote robustness in gene-expression networks... - PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegansPedro J Batista
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Cell 31:67-78. 2008..Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility... - The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegansMartha C Soto
Program in Molecular Medicine and Cell Biology, Howard Hughes Medical Institute, University of Massachusetts Cancer Center, Worcester, Massachusetts 01605, USA
Genes Dev 16:620-32. 2002..Our findings suggest that GEX-2 and GEX-3 may function at cell boundaries to regulate cell migrations and cell shape changes required for proper morphogenesis and development... - The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosisAkira Hayakawa
Program in Molecular Medicine and Biomedical Imaging Group, Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01615, USA
Proc Natl Acad Sci U S A 103:11928-33. 2006.... 
Primal RNAs: The end of the beginning?Darryl Conte
University of Massachusetts Medical School, Worcester, 01605, USA
Cell 140:452-4. 2010..But which comes first? Halic and Moazed (2010) propose that primal small RNAs initiate the amplification of small interfering RNAs that drive heterochromatin formation and chromatin silencing...- [Discovery of mechanisms behind genetic regulation awarded with Nobel Prize]Peter Orn
Lakartidningen 103:2937. 2006 - Functional conservation between members of an ancient duplicated transcription factor family, LSF/GrainyheadKavitha Venkatesan
Bioinformatics Program, Boston University, Boston, MA 02215, USA
Nucleic Acids Res 31:4304-16. 2003..e. to the time when the first multicellular organisms are thought to have arisen... - Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutantsDuo Wang
Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
Nature 436:593-7. 2005..These findings suggest that mutations in Rb pathway components cause cells to revert to patterns of gene expression normally restricted to germ cells. Rb may act by a similar mechanism to transform mammalian cells... - Return to the RNAi world: rethinking gene expression and evolutionCraig C Mello
Cell Death Differ 14:2013-20. 2007..Finally, Professor Mello leaves us with a number of questions that his research has raised and that will require years of future research to be answered... - Fundamental genetic findings net NobelsBridget M Kuehn
JAMA 296:2189-90. 2006 - [The Nobel Prize in Physiology or Medicine for 2006 for the discovery of RNA interference]R Bernards
Nederlands Kanker Instituut Antoni van Leeuwenhoek Ziekenhuis, afd Moleculaire Carcinogenese, Amsterdam
Ned Tijdschr Geneeskd 150:2849-53. 2006The Nobel Prize in Physiology or Medicine has been awarded to Andrew Fire and Craig Mello for their discovery of RNA interference, i.e. the suppression of gene activity by double-stranded RNA... - A conversation with Craig C Mello on the discovery of RNAiCraig C Mello
Cell Death Differ 14:1981-4. 2007 - Inducible systemic RNA silencing in Caenorhabditis elegansLisa Timmons
Department of Molecular Biosciences, The University of Kansas, Lawrence, Kansas 66045, USA
Mol Biol Cell 14:2972-83. 2003..elegans and can separate mechanisms underlying systemic RNA silencing into tissue-specific components. These data suggest that trafficking of RNA silencing signals in C. elegans is regulated by specific physiological and genetic factors... - [Regulation of eukaryotic gene expression and the Nobel prizes 2006]Goran Brajulković
Vojnosanit Pregl 63:993-4. 2006 - Chromatin regulation during C. elegans germline developmentTae Ho Shin
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Curr Opin Genet Dev 13:455-62. 2003..The germline, in turn, may rely on an asymmetrically inherited inhibitor to prevent chromatin reorganization that would otherwise erase pluripotency... - Micromanaging insulin secretionCraig C Mello
Nat Med 10:1297-8. 2004 - RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegansBastiaan B J Tops
Hubrecht Laboratory, Centre for Biomedical Genetics Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Nucleic Acids Res 33:347-55. 2005..Together these data support a role for the MUT-7/RDE-2 complex downstream of siRNA formation, but upstream of siRNA mediated target RNA recognition, possibly indicating a role in the siRNA amplification step... - [Nobel Prize for RNA interference]Ola Snøve
Tidsskr Nor Laegeforen 126:3252. 2006 - [RNAi, Fire (see text) Mello]Haruhiko Siomi
Tanpakushitsu Kakusan Koso 51:2352-5. 2006
Research Grants
- Cell Polarity Signaling in C. elegans EmbryosCraig Mello; Fiscal Year: 2007..The significance of this work lies in the opportunity it provides to study important regulators of cellular polarity within a relatively simple and well characterized genetic model system. ..
- RNA Mediated Genetic Interference in C. elegansCraig Mello; Fiscal Year: 2007..Moreover, the genetic mechanisms of RNAi are related to ancient gene-regulatory mechanisms and are thus likely to be relevant to many aspects of human development and disease. ..
- RNA Mediated Genetic Interference in C. elegansCraig C Mello; Fiscal Year: 2010..Moreover, the genetic mechanisms of RNAi are related to ancient gene-regulatory mechanisms and are thus likely to be relevant to many aspects of human development and disease. ..
- RNAi and Related PathwaysCraig Mello; Fiscal Year: 2005....
- RNA Mediated Genetic Interference in C. elegansCraig Mello; Fiscal Year: 2006..Moreover, because the genetic mechanisms of RNAi are related to ancient gene-regulatory mechanisms, the proposed studies will lead to new insights of potential importance to our understanding of human development and disease. ..
- RNA MEDIATED GENETIC INTERFERENCE IN C ELEGANSCraig Mello; Fiscal Year: 2002..elegans genes whose projects mediate genetic interference. Insights from these studies may lead directly to the development of related RNA interference technologies for probing gene function in other organisms including humans. ..
- CELL POLARITY SIGNALING IN C ELEGANS EMBRYOSCraig Mello; Fiscal Year: 2003..The significance of this work lies in the opportunity it provides to study important regulators of cellular polarity within a relatively simple and well characterized genetic model system. ..