Craig Mello

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. ncbi request reprint Revealing the world of RNA interference
    Craig C Mello
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 431:338-42. 2004
  2. pmc Return to the RNAi world: rethinking gene expression and evolution (Nobel Lecture)
    Craig C Mello
    Howard Hughes Medical Institute and Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Angew Chem Int Ed Engl 46:6985-94. 2007
  3. pmc Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline
    Weifeng Gu
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA
    Mol Cell 36:231-44. 2009
  4. ncbi request reprint The Conserved Kinases CDK-1, GSK-3, KIN-19, and MBK-2 Promote OMA-1 Destruction to Regulate the Oocyte-to-Embryo Transition in C. elegans
    Masaki Shirayama
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, Massachusetts 01605
    Curr Biol 16:47-55. 2006
  5. ncbi request reprint SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C. elegans embryos
    Yanxia Bei
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Dev Cell 3:113-25. 2002
  6. ncbi request reprint Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways
    Thomas F Duchaine
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01605, USA
    Cell 124:343-54. 2006
  7. ncbi request reprint Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi
    Erbay Yigit
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 127:747-57. 2006
  8. pmc NMY-2 maintains cellular asymmetry and cell boundaries, and promotes a SRC-dependent asymmetric cell division
    Ji Liu
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Biol 339:366-73. 2010
  9. pmc Sequence-specific inhibition of small RNA function
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Biol 2:E98. 2004
  10. ncbi request reprint RNA interference: big applause for silencing in Stockholm
    Phillip D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 127:1083-6. 2006

Research Grants

  1. Cell Polarity Signaling in C. elegans Embryos
    Craig Mello; Fiscal Year: 2007
  2. RNA Mediated Genetic Interference in C. elegans
    Craig Mello; Fiscal Year: 2007
  3. RNA Mediated Genetic Interference in C. elegans
    Craig C Mello; Fiscal Year: 2010
  4. RNAi and Related Pathways
    Craig Mello; Fiscal Year: 2005
  5. RNA Mediated Genetic Interference in C. elegans
    Craig Mello; Fiscal Year: 2006
  6. RNA MEDIATED GENETIC INTERFERENCE IN C ELEGANS
    Craig Mello; Fiscal Year: 2002
  7. CELL POLARITY SIGNALING IN C ELEGANS EMBRYOS
    Craig Mello; Fiscal Year: 2003

Collaborators

  • H Tabara
  • Evgeny Rogaev
  • JAMES CARRINGTON
  • Phillip D Zamore
  • Alla Grishok
  • Lisa L Maduzia
  • Haruhiko Siomi
  • Kozo Kaibuchi
  • L A Berkowitz
  • Rene F Ketting
  • David Bartel
  • GARY B RUVKUN
  • Sander van den Heuvel
  • Ji Liu
  • Andrew Z Fire
  • R H Plasterk
  • LISA D TIMMONS
  • Rene Bernards
  • Darryl Conte
  • Pedro J Batista
  • Masaki Shirayama
  • Julie M Claycomb
  • Weifeng Gu
  • Yanxia Bei
  • Jessica J Vasale
  • Daniel A Chaves
  • Ka Ming Pang
  • Shohei Mitani
  • Chun Chieh G Chen
  • Kuniaki Nakamura
  • Martha C Soto
  • Thomas F Duchaine
  • Daniel R Brownell
  • Takao Ishidate
  • Martin J Simard
  • Colin C Conine
  • Elaine M Youngman
  • Tae Ho Shin
  • Shenghua Duan
  • Kristin D Kasschau
  • Noah Fahlgren
  • John R Yates
  • Peter Orn
  • Erbay Yigit
  • Goran Brajulković
  • Bridget M Kuehn
  • Ola Snøve
  • Akira Hayakawa
  • Scott Kennedy
  • Kaming Pang
  • Soyoung Kim
  • Bastiaan B J Tops
  • Duo Wang
  • Chris Trzepacz
  • Martha Soto
  • Anastasia P Grigorenko
  • Gyorgy Hutvagner
  • Kavitha Venkatesan
  • Yingdee Unhavaithaya
  • Caroline Thivierge
  • Josien C Van Wolfswinkel
  • Jennifer Keys
  • Matthew J Stoltz
  • Jessica Vasale
  • James J Moresco
  • J Graham Ruby
  • Rosaria Chiang
  • Shujun Luo
  • Gary P Schroth
  • Kevin Fogarty
  • Karl Bellve
  • Silvia Corvera
  • Leemor Joshua-Tor
  • Sandra Harding
  • Pal Saetrom
  • James A Wohlschlegel
  • Susan Hayes
  • Niraj H Tolia
  • Deborah Leonard
  • Snezana Cerovic
  • David Lambright
  • Clive Standley
  • Stephanie Murphy
  • Harrison W Gabel
  • Titia Sijen
  • Jennifer A McCollough
  • Ravi S Kamath
  • Femke Simmer
  • John K Kim
  • Charlotte M Schubert

Detail Information

Publications40

  1. ncbi request reprint Revealing the world of RNA interference
    Craig C Mello
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nature 431:338-42. 2004
    ..At the heart of RNA interference lies a remarkable RNA processing mechanism that is now known to underlie many distinct biological phenomena...
  2. pmc Return to the RNAi world: rethinking gene expression and evolution (Nobel Lecture)
    Craig C Mello
    Howard Hughes Medical Institute and Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Angew Chem Int Ed Engl 46:6985-94. 2007
  3. pmc Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline
    Weifeng Gu
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA
    Mol Cell 36:231-44. 2009
    ..These findings broaden our understanding of the biogenesis and diversity of 22G-RNAs and suggest additional regulatory functions for small RNAs...
  4. ncbi request reprint The Conserved Kinases CDK-1, GSK-3, KIN-19, and MBK-2 Promote OMA-1 Destruction to Regulate the Oocyte-to-Embryo Transition in C. elegans
    Masaki Shirayama
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, Massachusetts 01605
    Curr Biol 16:47-55. 2006
    ..The asymmetric localization of these and other determinants is regulated in early embryos through motor-dependent physical translocation as well as selective proteolysis...
  5. ncbi request reprint SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C. elegans embryos
    Yanxia Bei
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Dev Cell 3:113-25. 2002
    ..Our findings suggest that Wnt and Src signaling function in parallel to control developmental outcomes within a single responding cell...
  6. ncbi request reprint Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways
    Thomas F Duchaine
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01605, USA
    Cell 124:343-54. 2006
    ..Our findings provide a first glimpse at the complex biochemical niche of Dicer and suggest that competition exists between DCR-1-mediated small-RNA pathways...
  7. ncbi request reprint Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi
    Erbay Yigit
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 127:747-57. 2006
    ..Interestingly, these AGO proteins lack key residues required for mRNA cleavage. Our findings support a two-step model for RNAi, in which functionally and structurally distinct AGOs act sequentially to direct gene silencing...
  8. pmc NMY-2 maintains cellular asymmetry and cell boundaries, and promotes a SRC-dependent asymmetric cell division
    Ji Liu
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Biol 339:366-73. 2010
    ..Finally, during a signaling-induced asymmetric cell division, NMY-2 is required for SRC-dependent phosphotyrosine signaling and acts in parallel with WNT-signaling to specify endoderm...
  9. pmc Sequence-specific inhibition of small RNA function
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Biol 2:E98. 2004
    ....
  10. ncbi request reprint RNA interference: big applause for silencing in Stockholm
    Phillip D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 127:1083-6. 2006
    Eight years ago, Craig Mello, Andrew Fire, and their coworkers provided the first demonstration that double-stranded RNA (dsRNA) triggers the gene-silencing technique that we now call RNA interference (RNAi)...
  11. pmc The Caenorhabditis elegans IMPAS gene, imp-2, is essential for development and is functionally distinct from related presenilins
    Anastasia P Grigorenko
    Department of Psychiatry, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, 303 Belmont Street, Worcester, MA 01604, USA
    Proc Natl Acad Sci U S A 101:14955-60. 2004
    ..These data suggest that IMPs are functionally distinct from related PSs and implicate IMPs as critical regulators of development that may potentially interact with the lipid-lipoprotein receptor-mediated pathway...
  12. pmc Sequential rounds of RNA-dependent RNA transcription drive endogenous small-RNA biogenesis in the ERGO-1/Argonaute pathway
    Jessica J Vasale
    Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 107:3582-7. 2010
    ..ERGO-1 targets exhibit a nonrandom distribution in the genome and appear to include many gene duplications, suggesting that this pathway may control overexpression resulting from gene expansion...
  13. ncbi request reprint A member of the polymerase beta nucleotidyltransferase superfamily is required for RNA interference in C. elegans
    Chun Chieh G Chen
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Curr Biol 15:378-83. 2005
    ..These findings implicate a new enzymatic modality in RNAi and suggest possible models for the role of RDE-3 in the RNAi mechanism...
  14. ncbi request reprint The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegans
    Hiroaki Tabara
    Program in Molecular Medicine, University of Massachusetts Meidcal School, Worcester, MA 1605, USA
    Cell 109:861-71. 2002
    ..Our findings suggest a model in which RDE-4 and RDE-1 function together to detect and retain foreign dsRNA and to present this dsRNA to DCR-1 for processing...
  15. pmc Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos
    Kuniaki Nakamura
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 19:1749-54. 2005
    ..We show that both PRY-1/Axin and the nuclear exportin homolog IMB-4/CRM-1 antagonize signaling. These findings reveal how Wnt signals direct the asymmetric localization of beta-catenin during polarized cell division...
  16. doi request reprint RNA interference in Caenorhabditis elegans
    Darryl Conte
    University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Curr Protoc Mol Biol . 2003
    ..RNAi has become an essential tool in C. elegans research. Procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA and soaking in dsRNA solution are described in this unit...
  17. ncbi request reprint RNAi (Nematodes: Caenorhabditis elegans)
    Alla Grishok
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester 01605, USA
    Adv Genet 46:339-60. 2002
    ..We discuss RNAi-deficient mutants in C. elegans as well as characterized components of the RNAi, pathway, the molecular mechanism of RNAi, and its possible role in development and immunity...
  18. ncbi request reprint MEP-1 and a homolog of the NURD complex component Mi-2 act together to maintain germline-soma distinctions in C. elegans
    Yingdee Unhavaithaya
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 111:991-1002. 2002
    ....
  19. ncbi request reprint The minibrain kinase homolog, mbk-2, is required for spindle positioning and asymmetric cell division in early C. elegans embryos
    Ka Ming Pang
    Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Biol 265:127-39. 2004
    ..These findings in conjunction with work from Schizosaccharomyces pombe indicate a possible conserved role for Dyrk family kinases in the regulation of spindle placement during cell division...
  20. pmc PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans
    Pedro J Batista
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 31:67-78. 2008
    ..Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility...
  21. pmc The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregation
    Julie M Claycomb
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01606, USA
    Cell 139:123-34. 2009
    ..Instead, our findings support a model in which CSR-1 complexes target protein-coding domains to promote their proper organization within the holocentric chromosomes of C. elegans...
  22. pmc Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans
    Colin C Conine
    The Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01606, USA
    Proc Natl Acad Sci U S A 107:3588-93. 2010
    ..These findings add to a growing number of AGO pathways required for thermotolerant fertility in C. elegans and support a model in which AGOs and their small RNA cofactors function to promote robustness in gene-expression networks...
  23. pmc The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans
    Martha C Soto
    Program in Molecular Medicine and Cell Biology, Howard Hughes Medical Institute, University of Massachusetts Cancer Center, Worcester, Massachusetts 01605, USA
    Genes Dev 16:620-32. 2002
    ..Our findings suggest that GEX-2 and GEX-3 may function at cell boundaries to regulate cell migrations and cell shape changes required for proper morphogenesis and development...
  24. pmc The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosis
    Akira Hayakawa
    Program in Molecular Medicine and Biomedical Imaging Group, Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01615, USA
    Proc Natl Acad Sci U S A 103:11928-33. 2006
    ....
  25. doi request reprint Primal RNAs: The end of the beginning?
    Darryl Conte
    University of Massachusetts Medical School, Worcester, 01605, USA
    Cell 140:452-4. 2010
    ..But which comes first? Halic and Moazed (2010) propose that primal small RNAs initiate the amplification of small interfering RNAs that drive heterochromatin formation and chromatin silencing...
  26. pmc Functional conservation between members of an ancient duplicated transcription factor family, LSF/Grainyhead
    Kavitha Venkatesan
    Bioinformatics Program, Boston University, Boston, MA 02215, USA
    Nucleic Acids Res 31:4304-16. 2003
    ..e. to the time when the first multicellular organisms are thought to have arisen...
  27. ncbi request reprint Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants
    Duo Wang
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 436:593-7. 2005
    ..These findings suggest that mutations in Rb pathway components cause cells to revert to patterns of gene expression normally restricted to germ cells. Rb may act by a similar mechanism to transform mammalian cells...
  28. ncbi request reprint Fundamental genetic findings net Nobels
    Bridget M Kuehn
    JAMA 296:2189-90. 2006
  29. ncbi request reprint Return to the RNAi world: rethinking gene expression and evolution
    Craig C Mello
    Cell Death Differ 14:2013-20. 2007
    ..Finally, Professor Mello leaves us with a number of questions that his research has raised and that will require years of future research to be answered...
  30. ncbi request reprint [The Nobel Prize in Physiology or Medicine for 2006 for the discovery of RNA interference]
    R Bernards
    Nederlands Kanker Instituut Antoni van Leeuwenhoek Ziekenhuis, afd Moleculaire Carcinogenese, Amsterdam
    Ned Tijdschr Geneeskd 150:2849-53. 2006
    The Nobel Prize in Physiology or Medicine has been awarded to Andrew Fire and Craig Mello for their discovery of RNA interference, i.e. the suppression of gene activity by double-stranded RNA...
  31. ncbi request reprint [Discovery of mechanisms behind genetic regulation awarded with Nobel Prize]
    Peter Orn
    Lakartidningen 103:2937. 2006
  32. ncbi request reprint A conversation with Craig C Mello on the discovery of RNAi
    Craig C Mello
    Cell Death Differ 14:1981-4. 2007
  33. ncbi request reprint [Nobel Prize for RNA interference]
    Ola Snøve
    Tidsskr Nor Laegeforen 126:3252. 2006
  34. pmc Inducible systemic RNA silencing in Caenorhabditis elegans
    Lisa Timmons
    Department of Molecular Biosciences, The University of Kansas, Lawrence, Kansas 66045, USA
    Mol Biol Cell 14:2972-83. 2003
    ..elegans and can separate mechanisms underlying systemic RNA silencing into tissue-specific components. These data suggest that trafficking of RNA silencing signals in C. elegans is regulated by specific physiological and genetic factors...
  35. ncbi request reprint Chromatin regulation during C. elegans germline development
    Tae Ho Shin
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Opin Genet Dev 13:455-62. 2003
    ..The germline, in turn, may rely on an asymmetrically inherited inhibitor to prevent chromatin reorganization that would otherwise erase pluripotency...
  36. ncbi request reprint Micromanaging insulin secretion
    Craig C Mello
    Nat Med 10:1297-8. 2004
  37. pmc RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans
    Bastiaan B J Tops
    Hubrecht Laboratory, Centre for Biomedical Genetics Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nucleic Acids Res 33:347-55. 2005
    ..Together these data support a role for the MUT-7/RDE-2 complex downstream of siRNA formation, but upstream of siRNA mediated target RNA recognition, possibly indicating a role in the siRNA amplification step...
  38. ncbi request reprint [Regulation of eukaryotic gene expression and the Nobel prizes 2006]
    Goran Brajulković
    Vojnosanit Pregl 63:993-4. 2006
  39. ncbi request reprint [RNAi, Fire (see text) Mello]
    Haruhiko Siomi
    Tanpakushitsu Kakusan Koso 51:2352-5. 2006

Research Grants23

  1. Cell Polarity Signaling in C. elegans Embryos
    Craig Mello; Fiscal Year: 2007
    ..The significance of this work lies in the opportunity it provides to study important regulators of cellular polarity within a relatively simple and well characterized genetic model system. ..
  2. RNA Mediated Genetic Interference in C. elegans
    Craig Mello; Fiscal Year: 2007
    ..Moreover, the genetic mechanisms of RNAi are related to ancient gene-regulatory mechanisms and are thus likely to be relevant to many aspects of human development and disease. ..
  3. RNA Mediated Genetic Interference in C. elegans
    Craig C Mello; Fiscal Year: 2010
    ..Moreover, the genetic mechanisms of RNAi are related to ancient gene-regulatory mechanisms and are thus likely to be relevant to many aspects of human development and disease. ..
  4. RNAi and Related Pathways
    Craig Mello; Fiscal Year: 2005
    ....
  5. RNA Mediated Genetic Interference in C. elegans
    Craig Mello; Fiscal Year: 2006
    ..Moreover, because the genetic mechanisms of RNAi are related to ancient gene-regulatory mechanisms, the proposed studies will lead to new insights of potential importance to our understanding of human development and disease. ..
  6. RNA MEDIATED GENETIC INTERFERENCE IN C ELEGANS
    Craig Mello; Fiscal Year: 2002
    ..elegans genes whose projects mediate genetic interference. Insights from these studies may lead directly to the development of related RNA interference technologies for probing gene function in other organisms including humans. ..
  7. CELL POLARITY SIGNALING IN C ELEGANS EMBRYOS
    Craig Mello; Fiscal Year: 2003
    ..The significance of this work lies in the opportunity it provides to study important regulators of cellular polarity within a relatively simple and well characterized genetic model system. ..