Eugene Melamud

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. pmc Stochastic noise in splicing machinery
    Eugene Melamud
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 37:4873-86. 2009
  2. pmc Structural implication of splicing stochastics
    Eugene Melamud
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 37:4862-72. 2009
  3. ncbi request reprint Characterization, kinetics, and crystal structures of fructose-1,6-bisphosphate aldolase from the human parasite, Giardia lamblia
    Andrey Galkin
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    J Biol Chem 282:4859-67. 2007
  4. pmc Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein
    Nozomi Sakakibara
    University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 36:1309-20. 2008
  5. ncbi request reprint Crystal structures of 2-methylisocitrate lyase in complex with product and with isocitrate inhibitor provide insight into lyase substrate specificity, catalysis and evolution
    Sijiu Liu
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Biochemistry 44:2949-62. 2005
  6. pmc The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses
    Barbara A Methe
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 102:10913-8. 2005
  7. ncbi request reprint Biochemical characterization of the Methanothermobacter thermautotrophicus minichromosome maintenance (MCM) helicase N-terminal domains
    Rajesh Kasiviswanathan
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    J Biol Chem 279:28358-66. 2004
  8. ncbi request reprint Evaluation of disorder predictions in CASP5
    Eugene Melamud
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Proteins 53:561-5. 2003
  9. ncbi request reprint Assisting functional assignment for hypothetical Heamophilus influenzae gene products through structural genomics
    Gary L Gilliland
    Center for Advanced Research in Biotechnology of the University of Maryland Biotechnology Institute and the National Institute of Standards and Technology, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Curr Drug Targets Infect Disord 2:339-53. 2002
  10. pmc SNPs3D: candidate gene and SNP selection for association studies
    Peng Yue
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
    BMC Bioinformatics 7:166. 2006

Collaborators

Detail Information

Publications11

  1. pmc Stochastic noise in splicing machinery
    Eugene Melamud
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 37:4873-86. 2009
    ..Based on simulation of sampling of virtual cDNA libraries, we estimate that error rates range from 1 to 10% depending on the number of introns and the expression level of a gene...
  2. pmc Structural implication of splicing stochastics
    Eugene Melamud
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 37:4862-72. 2009
    ..Overall, the properties of alternative spliced proteins are consistent with the outcome of noisy selection of splice sites by splicing machinery...
  3. ncbi request reprint Characterization, kinetics, and crystal structures of fructose-1,6-bisphosphate aldolase from the human parasite, Giardia lamblia
    Andrey Galkin
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    J Biol Chem 282:4859-67. 2007
    ..Determinants of catalysis and substrate recognition, derived from comparison of the G. lamblia FBPA structure with Escherichia coli FBPA and with a closely related enzyme, E. coli tagatose-1,6-bisphosphate aldolase (TBPA), are described...
  4. pmc Coupling of DNA binding and helicase activity is mediated by a conserved loop in the MCM protein
    Nozomi Sakakibara
    University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 36:1309-20. 2008
    ..Since similar residues are also conserved in the eukaryotic MCM proteins, the data presented here suggest a similar coupling between the N-terminal and catalytic domain of the eukaryotic enzyme...
  5. ncbi request reprint Crystal structures of 2-methylisocitrate lyase in complex with product and with isocitrate inhibitor provide insight into lyase substrate specificity, catalysis and evolution
    Sijiu Liu
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Biochemistry 44:2949-62. 2005
    ..Structure-based sequence analysis and phylogenic tree construction reveal determinants of substrate specificity, highlight nodes of divergence of families, and predict enzyme families with new functions...
  6. pmc The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses
    Barbara A Methe
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 102:10913-8. 2005
    ..Comparative genome analyses suggest that the psychrophilic lifestyle is most likely conferred not by a unique set of genes but by a collection of synergistic changes in overall genome content and amino acid composition...
  7. ncbi request reprint Biochemical characterization of the Methanothermobacter thermautotrophicus minichromosome maintenance (MCM) helicase N-terminal domains
    Rajesh Kasiviswanathan
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    J Biol Chem 279:28358-66. 2004
    ..It is also shown that although oligomerization is not essential for single-stranded DNA binding and ATPase activity, the presence of domain C is essential for helicase activity...
  8. ncbi request reprint Evaluation of disorder predictions in CASP5
    Eugene Melamud
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Proteins 53:561-5. 2003
    ..Over-all, the methods are clearly of considerable use in identifying potential disorder...
  9. ncbi request reprint Assisting functional assignment for hypothetical Heamophilus influenzae gene products through structural genomics
    Gary L Gilliland
    Center for Advanced Research in Biotechnology of the University of Maryland Biotechnology Institute and the National Institute of Standards and Technology, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Curr Drug Targets Infect Disord 2:339-53. 2002
    ..In addition to aiding in functional assignment, this effort is identifying a number of possible new targets for drug development...
  10. pmc SNPs3D: candidate gene and SNP selection for association studies
    Peng Yue
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA
    BMC Bioinformatics 7:166. 2006
    ..We describe a web resource and database that provides and integrates as much information as possible on disease/gene relationships at the molecular level...
  11. ncbi request reprint The HI0073/HI0074 protein pair from Haemophilus influenzae is a member of a new nucleotidyltransferase family: structure, sequence analyses, and solution studies
    Christopher Lehmann
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Proteins 50:249-60. 2003
    ..Double- and single-stranded DNA binding assays showed no evidence of DNA binding to HI0074 or to HI0073/HI0074 complex despite the suggestive shape of the putative binding cleft formed by the HI0074 dimer...