J Richard McIntosh

Summary

Affiliation: University of Colorado
Country: USA

Publications

  1. doi request reprint Biophysics of mitosis
    J Richard McIntosh
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Q Rev Biophys 45:147-207. 2012
  2. ncbi request reprint Measurement of the force developed by disassembling microtubule during calcium-induced depolymerization
    M I Molodtsov
    Hematology Research Center, Russian Academy of Medical Sciences, Moscow 125167, Russia
    Dokl Biochem Biophys 412:18-21. 2007
  3. pmc Conserved and divergent features of kinetochores and spindle microtubule ends from five species
    J Richard McIntosh
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Cell Biol 200:459-74. 2013
  4. pmc Lattice structure of cytoplasmic microtubules in a cultured Mammalian cell
    J Richard McIntosh
    Laboratory for 3D Structure of Cells and Molecules, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    J Mol Biol 394:177-82. 2009
  5. ncbi request reprint New views of cells in 3D: an introduction to electron tomography
    Richard McIntosh
    Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Trends Cell Biol 15:43-51. 2005
  6. pmc Tubulin depolymerization may be an ancient biological motor
    J Richard McIntosh
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Cell Sci 123:3425-34. 2010
  7. pmc Mitosis futures: the past is prologue
    J Richard McIntosh
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Mol Biol Cell 22:3933-5. 2011
  8. ncbi request reprint Chromosome-microtubule interactions during mitosis
    J Richard McIntosh
    Department of Molecular Cellular and Developmental Biology, University of Colorado, Boulder 80309 0347, USA
    Annu Rev Cell Dev Biol 18:193-219. 2002
  9. pmc Fibrils connect microtubule tips with kinetochores: a mechanism to couple tubulin dynamics to chromosome motion
    J Richard McIntosh
    Department of M C D Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Cell 135:322-33. 2008
  10. pmc pkl1(+)and klp2(+): Two kinesins of the Kar3 subfamily in fission yeast perform different functions in both mitosis and meiosis
    C L Troxell
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Mol Biol Cell 12:3476-88. 2001

Research Grants

  1. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2000
  2. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2001
  3. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2002
  4. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2003
  5. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2004
  6. Kinetochore-Microtubule Interactions in Fission Yeast
    J McIntosh; Fiscal Year: 2005
  7. Kinetochore-Microtubule Interactions in Fission Yeast
    J McIntosh; Fiscal Year: 2006
  8. Kinetochore-Microtubule Interactions in Fission Yeast
    J McIntosh; Fiscal Year: 2007
  9. Kinetochore-Microtubule Interactions
    J McIntosh; Fiscal Year: 2009
  10. Kinetochore-Microtubule Interactions
    JOHN RICHARD MCINTOSH; Fiscal Year: 2010

Collaborators

Detail Information

Publications62

  1. doi request reprint Biophysics of mitosis
    J Richard McIntosh
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Q Rev Biophys 45:147-207. 2012
    ..Our goal is to provide a document that will expose biophysicists to the fascination of this quite amazing process and provide them with a good background from which they can pursue their own research interests in the subject...
  2. ncbi request reprint Measurement of the force developed by disassembling microtubule during calcium-induced depolymerization
    M I Molodtsov
    Hematology Research Center, Russian Academy of Medical Sciences, Moscow 125167, Russia
    Dokl Biochem Biophys 412:18-21. 2007
  3. pmc Conserved and divergent features of kinetochores and spindle microtubule ends from five species
    J Richard McIntosh
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Cell Biol 200:459-74. 2013
    ..Flaring protofilaments linked to chromatin are well placed to exert force on chromosomes, assuring stable attachment and reliable anaphase segregation...
  4. pmc Lattice structure of cytoplasmic microtubules in a cultured Mammalian cell
    J Richard McIntosh
    Laboratory for 3D Structure of Cells and Molecules, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    J Mol Biol 394:177-82. 2009
    ..It follows that cytoplasmic MTs are not cylindrically symmetric; they have two distinct faces, which may influence the binding patterns of functionally significant MT-interacting proteins...
  5. ncbi request reprint New views of cells in 3D: an introduction to electron tomography
    Richard McIntosh
    Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Trends Cell Biol 15:43-51. 2005
    ..Electron tomography can provide such information about many kinds of cells and organelles...
  6. pmc Tubulin depolymerization may be an ancient biological motor
    J Richard McIntosh
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Cell Sci 123:3425-34. 2010
    ..Mechanical similarities between these force-generation systems suggest a deep phylogenetic relationship between tubulin depolymerization in eukaryotic mitosis and FtsZ-mediated ring contraction in bacteria...
  7. pmc Mitosis futures: the past is prologue
    J Richard McIntosh
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Mol Biol Cell 22:3933-5. 2011
    ....
  8. ncbi request reprint Chromosome-microtubule interactions during mitosis
    J Richard McIntosh
    Department of Molecular Cellular and Developmental Biology, University of Colorado, Boulder 80309 0347, USA
    Annu Rev Cell Dev Biol 18:193-219. 2002
    ..We conclude that microtubule dynamics and the proteins that control them are likely to be more important for mitosis than the current enthusiasm for motor enzymes would suggest...
  9. pmc Fibrils connect microtubule tips with kinetochores: a mechanism to couple tubulin dynamics to chromosome motion
    J Richard McIntosh
    Department of M C D Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Cell 135:322-33. 2008
    ..These analyses define a ring-independent mechanism for harnessing microtubule dynamics directly to chromosome movement...
  10. pmc pkl1(+)and klp2(+): Two kinesins of the Kar3 subfamily in fission yeast perform different functions in both mitosis and meiosis
    C L Troxell
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Mol Biol Cell 12:3476-88. 2001
    ..The anaphase spindle of klp2 Delta becomes longer than the cell, leading it to curl around the cell's ends. Apparently, Klp2p promotes spindle disassembly and contributes to the behavior of mitotic chromosomes...
  11. pmc Kinesin-8 from fission yeast: a heterodimeric, plus-end-directed motor that can couple microtubule depolymerization to cargo movement
    Paula M Grissom
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Mol Biol Cell 20:963-72. 2009
    ....
  12. pmc In search of an optimal ring to couple microtubule depolymerization to processive chromosome motions
    Artem Efremov
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Proc Natl Acad Sci U S A 104:19017-22. 2007
    ..Our analysis identifies key experimental parameters whose values should determine whether the Dam1/DASH ring moves via diffusion or a forced walk...
  13. pmc Novel interactions of fission yeast kinesin 8 revealed through in vivo expression of truncation alleles
    Robert R West
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Cell Motil Cytoskeleton 65:626-40. 2008
    ..Our results indicate that the localization signals for these kinesins are not derived from simple amino acid sequences but from complex interactions among multiple domains of each motor...
  14. pmc The Dam1 ring binds microtubules strongly enough to be a processive as well as energy-efficient coupler for chromosome motion
    Ekaterina L Grishchuk
    Molecular, Cellular, and Developmental Biology Department, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 105:15423-8. 2008
    ..Comparison of the forces generated against wild-type and mutant complexes confirms the importance of tight Dam1-MT association for processive cargo movement under load...
  15. pmc Mitotic chromosome biorientation in fission yeast is enhanced by dynein and a minus-end-directed, kinesin-like protein
    Ekaterina L Grishchuk
    Molecular, Cellular, and Developmental Biology Department, University of Colorado at Boulder, Boulder, CO 80309, USA
    Mol Biol Cell 18:2216-25. 2007
    ....
  16. pmc Tea2p is a kinesin-like protein required to generate polarized growth in fission yeast
    H Browning
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    J Cell Biol 151:15-28. 2000
    ..These results suggest that this kinesin promotes microtubule growth, possibly through interactions with the microtubule end, and that it is important for establishing and maintaining polarized growth along the long axis of the cell...
  17. pmc Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans
    Eileen T O'Toole
    Boulder Laboratory for 3 D Electron Microscopy of Cells, University of Colorado, 80309, USA
    J Cell Biol 163:451-6. 2003
    ..These results have structural implications for models of MT interactions with centrosomes...
  18. ncbi request reprint The molecular architecture of axonemes revealed by cryoelectron tomography
    Daniela Nicastro
    Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, CB 347, University of Colorado, Boulder, CO 80309 0347, USA
    Science 313:944-8. 2006
    ..Periodic densities were also observed inside doublet microtubules; these may contribute to doublet stability...
  19. ncbi request reprint Kinesins klp5(+) and klp6(+) are required for normal chromosome movement in mitosis
    Robert R West
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    J Cell Sci 115:931-40. 2002
    ..These data indicate that Klp5p and Klp6p are kinetochore kinesins required for normal chromosome movement in prometaphase...
  20. pmc Microtubule depolymerization can drive poleward chromosome motion in fission yeast
    Ekaterina L Grishchuk
    MCD Biology Department, University of Colorado at Boulder, Boulder, CO 80309, USA
    EMBO J 25:4888-96. 2006
    ..However, Klp2p, which localizes to kinetochores, contributed to the effectiveness of P-movement by promoting the shortening of kinetochore fibers...
  21. pmc A molecular-mechanical model of the microtubule
    Maxim I Molodtsov
    Molecular, Cellular, and Developmental Biology Department, University of Colorado, Boulder, Colorado, USA
    Biophys J 88:3167-79. 2005
    ..Overall, this model provides a new link between the biophysical characteristics of tubulin and the physiological behavior of MTs. It will also be useful in building a more complete description of MT dynamics and mechanics...
  22. pmc Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival
    Dewight R Williams
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, USA
    Eukaryot Cell 4:166-77. 2005
    ..We take these data to mean that Mcl1p is a dynamic component of the polymerase alpha complex during replication and is important for the replication stress response in fission yeast...
  23. pmc A screen for genes involved in the anaphase proteolytic pathway identifies tsm1(+), a novel Schizosaccharomyces pombe gene important for microtubule integrity
    E L Grishchuk
    Molecular Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Genetics 149:1251-64. 1998
    ..The chromosomes, however, often fail to segregate properly. Genetic interactions between tsm1-512 and components of the anaphase proteolytic pathway suggest a functional involvement of the Tsm1 protein in this pathway...
  24. pmc Three-dimensional organization of basal bodies from wild-type and delta-tubulin deletion strains of Chlamydomonas reinhardtii
    Eileen T O'Toole
    Boulder Laboratory for 3 D Fine Structure, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Mol Biol Cell 14:2999-3012. 2003
    ..These data suggest that the presence of specific tubulin isoforms in Chlamydomonas directly affects the assembly and function of both basal bodies and basal body-associated structures...
  25. ncbi request reprint A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles
    C Nislow
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309
    Nature 359:543-7. 1992
    ....
  26. pmc Cytoplasmic dynein plays a role in mammalian mitotic spindle formation
    E A Vaisberg
    Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder 80309 0347
    J Cell Biol 123:849-58. 1993
    ..Possible mechanisms of dynein's involvement in mitosis are discussed...
  27. pmc mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation
    Dewight R Williams
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Eukaryot Cell 1:758-73. 2002
    ..It interacts genetically with replication components and causes an S-phase delay when overexpressed. We propose that Mcl1p, like Ctf4p, has a role in regulating DNA replication complexes...
  28. pmc Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei
    Johanna L Höög
    The Boulder Laboratory for 3 D Electron Microscopy of Cells, MCD Biology, University of Colorado at Boulder, Boulder, CO 80309 0347, USA
    J Struct Biol 178:189-98. 2012
    ..Sub-tomogram averaging of the PFR revealed that its distal region is organized as an orthorhombic crystal...
  29. pmc Microtubule depolymerization by the Kinesin-8 motor Kip3p: a mathematical model
    L E Hough
    Physics Department, University of Colorado at Boulder, Boulder, Colorado, USA
    Biophys J 96:3050-64. 2009
    ....
  30. pmc Direct continuities between cisternae at different levels of the Golgi complex in glucose-stimulated mouse islet beta cells
    Brad J Marsh
    Boulder Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 101:5565-70. 2004
    ....
  31. pmc 3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography
    Daniela Nicastro
    Abteilung Molekulare Strukturbiologie, Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
    Proc Natl Acad Sci U S A 102:15889-94. 2005
    ..Cryo-electron tomography has proven to be a powerful technique for helping us understand the relationships between flagellar structure and function and the design of macromolecular machines in situ...
  32. pmc Different assemblies of the DAM1 complex follow shortening microtubules by distinct mechanisms
    E L Grishchuk
    Molecular, Cellular, and Developmental Biology Department, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 105:6918-23. 2008
    ..The coupling properties of these two assemblies are, however, quite different, so each may make a distinct contribution to chromosome motility...
  33. pmc High-voltage electron tomography of spindle pole bodies and early mitotic spindles in the yeast Saccharomyces cerevisiae
    E T O'Toole
    Boulder Laboratory for Three dimensional Fine Structure, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Mol Biol Cell 10:2017-31. 1999
    ..These studies complement and extend what is known about the three-dimensional structure of the yeast mitotic spindle and further our understanding of the organization of the SPB in intact cells...
  34. pmc Two related kinesins, klp5+ and klp6+, foster microtubule disassembly and are required for meiosis in fission yeast
    R R West
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Mol Biol Cell 12:3919-32. 2001
    ..Spore viability is low in homozygous crosses of either null mutant. Heterozygous crosses of klp5Delta with klp6Delta have an intermediate viability, suggesting cooperation between these proteins in meiosis...
  35. ncbi request reprint The use of filter membranes for high-pressure freezing of cell monolayers
    M K Morphew
    The Boulder Laboratory for 3 D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Microsc 212:21-5. 2003
    ..Our protocol results in good preservation of cellular ultrastructure, it is a useful companion to studies of cell physioloy and, with some limitation, is suitable for correlative LM and EM...
  36. pmc cut11(+): A gene required for cell cycle-dependent spindle pole body anchoring in the nuclear envelope and bipolar spindle formation in Schizosaccharomyces pombe
    R R West
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Mol Biol Cell 9:2839-55. 1998
    ..These data suggest that Cut11p associates with nuclear pore complexes and mitotic SPBs as an anchor in the nuclear envelope; this role is essential for mitosis...
  37. pmc Mammalian cells express three distinct dynein heavy chains that are localized to different cytoplasmic organelles
    E A Vaisberg
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309 0347, USA
    J Cell Biol 133:831-42. 1996
    ..Apparently, specific cytoplasmic dyneins, like individual members of the kinesin superfamily, play unique roles in the traffic of cytomembranes...
  38. ncbi request reprint Vitreous cryo-sectioning of cells facilitated by a micromanipulator
    Mark S Ladinsky
    Boulder Laboratory for Three Dimensional Electron Microscopy of Cells, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Microsc 224:129-34. 2006
    ..It even allows for serial cryo-electron microscopy. As such, this approach is an advance in the cryo-microtomy of vitreous sections...
  39. pmc Probing the macromolecular organization of cells by electron tomography
    Andreas Hoenger
    Boulder Laboratory for 3 Dimensional Electron Microscopy of Cells and Molecules, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Curr Opin Cell Biol 21:89-96. 2009
    ..Here, we describe recent progress in electron microscopy of cells and the ways in which the relevant methodologies are helping to elucidate cell architecture...
  40. pmc Silver enhancement of Nanogold particles during freeze substitution for electron microscopy
    M Morphew
    Laboratory for 3D Electron Microscopy of Cells, Department of M C D Biology, University of Colorado, Boulder, CO 80309 0347, USA
    J Microsc 230:263-7. 2008
    ..We then show results from application of the method to an in vivo system, using Nanogold to track the internalization of immunoglobulin by neonatal murine intestinal epithelium, a specific example of receptor-mediated membrane traffic...
  41. ncbi request reprint Cryo-fluorescence microscopy facilitates correlations between light and cryo-electron microscopy and reduces the rate of photobleaching
    Cindi L Schwartz
    Boulder Laboratory for 3D Electron Microscopy of Cells, University of Colorado, Department of Molecular, Cellular, and Developmental Biology, Boulder, CO, USA
    J Microsc 227:98-109. 2007
    ..Once located, the sample can be placed in the EM for further study at higher resolution. An additional benefit of the cryo-LM stage is that photobleaching is slower at cryogenic temperatures (-140 degrees C) than at room temperature...
  42. ncbi request reprint Single-strand DNA aptamers as probes for protein localization in cells
    Kristi K H Stanlis
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    J Histochem Cytochem 51:797-808. 2003
    ..We found that aptamers are sufficiently soluble in cold organic solvents to encourage the view that this approach may be useful for the localization of specific proteins in context of cellular fine structure...
  43. pmc Identification of a novel light intermediate chain (D2LIC) for mammalian cytoplasmic dynein 2
    Paula M Grissom
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Mol Biol Cell 13:817-29. 2002
    ..Our results suggest that D2LIC is a bona fide subunit of cytoplasmic dynein 2 that may play a role in maintaining Golgi organization by binding cytoplasmic dynein 2 to its Golgi-associated cargo...
  44. ncbi request reprint Electron tomography of yeast cells
    Eileen T O'Toole
    Boulder Laboratory for 3 D Fine Structure, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Methods Enzymol 351:81-95. 2002
  45. pmc Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography
    B J Marsh
    Boulder Laboratory for 3-D Fine Structure, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 98:2399-406. 2001
    ..We relate the intimacy of structural associations between organelles in the Golgi region, as quantified by spatial density analyses, to biochemical mechanisms for membrane trafficking and organellar communication in mammalian cells...
  46. ncbi request reprint Effects of the myosin inhibitor 2,3-butanedione monoxime on the physiology of fission yeast
    G Steinberg
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, USA
    Eur J Cell Biol 77:284-93. 1998
    ..Our results are consistent with the hypothesis that BDM influences actin-dependent processes in fission yeast and that actomyosin-dependent motility contributes to the secretory process of tip growth...
  47. ncbi request reprint Kinesin from the plant pathogenic fungus Ustilago maydis is involved in vacuole formation and cytoplasmic migration
    G Steinberg
    Department of M C D Biology, University of Colorado, Boulder, CO 80309, USA
    J Cell Sci 111:2235-46. 1998
    ..The accumulation of these vacuoles at the basal end of the tip cell might be crucial for the formation of the empty sections and supports cytoplasmic migration during the growth of dikaryotic hyphae...
  48. pmc Structure of the Golgi and distribution of reporter molecules at 20 degrees C reveals the complexity of the exit compartments
    Mark S Ladinsky
    Boulder Laboratory for 3 D Fine Structure, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309, USA
    Mol Biol Cell 13:2810-25. 2002
    ..These data suggest that exit from the Golgi occurs directly from three trans-cisternae and that specialized ER plays a significant role in trans-Golgi function...
  49. ncbi request reprint Structural evidence for multiple transport mechanisms through the Golgi in the pancreatic beta-cell line, HIT-T15
    B J Marsh
    Boulder Laboratory for 3-D Fine Structure, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Biochem Soc Trans 29:461-7. 2001
    ....
  50. pmc Force production by depolymerizing microtubules: a theoretical study
    M I Molodtsov
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 102:4353-8. 2005
    ....
  51. pmc Fabrication and characterization of a carbon nanotube-based nanoknife
    G Singh
    Department of Mechanical Engineering, University of Colorado at Boulder, CO 80309, USA
    Nanotechnology 20:095701. 2009
    ..Initial experiments show indentation marks on the epoxy surface. Quantitative analysis is currently limited by the surface asperities, which have the same dimensions as the nanotube...
  52. pmc A freeze substitution fixation-based gold enlarging technique for EM studies of endocytosed Nanogold-labeled molecules
    Wanzhong He
    Division of Biology 114 96, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    J Struct Biol 160:103-13. 2007
    ..These methods should be broadly applicable to many endocytosis and transcytosis studies...
  53. ncbi request reprint Rings around kinetochore microtubules in yeast
    J Richard McIntosh
    Nat Struct Mol Biol 12:210-2. 2005
  54. ncbi request reprint Organization of interphase microtubules in fission yeast analyzed by electron tomography
    Johanna L Höög
    European Molecular Biology Laboratory, Cell Biology and Biophysics Program, D 69117 Heidelberg, Germany
    Dev Cell 12:349-61. 2007
    ..Our analysis shows that electron tomography of well-preserved cells is ideally suited for describing fine ultrastructural details that were not visible with previous techniques...
  55. pmc FcRn-mediated antibody transport across epithelial cells revealed by electron tomography
    Wanzhong He
    Division of Biology 114 96, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, USA
    Nature 455:542-6. 2008
    ..Markers for early, late and recycling endosomes each labelled vesicles in different and overlapping morphological classes, revealing spatial complexity in endo-lysosomal trafficking...
  56. pmc The fission yeast NIMA kinase Fin1p is required for spindle function and nuclear envelope integrity
    Michael J E Krien
    Trescowthick Research Laboratories, Peter MacCallum Cancer Institute, Locked Bag 1, A Beckett Street, Melbourne, Victoria 8006, Australia
    EMBO J 21:1713-22. 2002
    ..These data support a role for Fin1p in spindle function and nuclear envelope transactions at or after the metaphase-anaphase transition that may be generally applicable to other NIMA-family members...
  57. ncbi request reprint Unstable kinetochore-microtubule capture and chromosomal instability following deletion of CENP-E
    Frances R Putkey
    Ludwig Institute for Cancer Research, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Dev Cell 3:351-65. 2002
    ..CENP-E is thus essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores...
  58. pmc Nuclear fusion during yeast mating occurs by a three-step pathway
    Patricia Melloy
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Cell Biol 179:659-70. 2007
    ..We conclude that nuclear fusion occurs by a three-step pathway...
  59. ncbi request reprint Force production by disassembling microtubules
    Ekaterina L Grishchuk
    MCD Biology Department, University of Colorado at Boulder, Colorado 80309 0347, USA
    Nature 438:384-8. 2005
    ..Because even the simple coupler used here slows MT disassembly, physiological couplers may modulate MT dynamics in vivo...
  60. pmc Cytoplasmic dynein nomenclature
    K Kevin Pfister
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Cell Biol 171:411-3. 2005
    ..This nomenclature recognizes the two distinct cytoplasmic dynein complexes and has the flexibility to accommodate the discovery of new subunits and isoforms...
  61. pmc A standardized kinesin nomenclature
    Carolyn J Lawrence
    Department of Plant Biology, The University of Georgia, Athens, GA 30602, USA
    J Cell Biol 167:19-22. 2004
    ..The scheme unifies all previous phylogenies and nomenclature proposals, while allowing individual sequence names to remain the same, and for expansion to occur as new sequences are discovered...

Research Grants27

  1. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2000
    ..Through this combination of studies we hope to identify the mitotic roles of several MT-dependent motor enzymes and learn how the enzymes interact with one another and with MT assembly to effect orderly chromosome motion. ..
  2. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2001
    ..g., tension) modify the physiology of the kinetochore ..
  3. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2002
    ..g., tension) modify the physiology of the kinetochore ..
  4. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2003
    ..g., tension) modify the physiology of the kinetochore ..
  5. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 2004
    ..g., tension) modify the physiology of the kinetochore ..
  6. Kinetochore-Microtubule Interactions in Fission Yeast
    J McIntosh; Fiscal Year: 2005
    ..The results from our work will inform biomedical science about important drug targets for cancer chemotherapy and genes that are significant for minimizing the risk of aneuploidy in both mitosis and meiosis. ..
  7. Kinetochore-Microtubule Interactions in Fission Yeast
    J McIntosh; Fiscal Year: 2006
    ..The results from our work will inform biomedical science about important drug targets for cancer chemotherapy and genes that are significant for minimizing the risk of aneuploidy in both mitosis and meiosis. ..
  8. Kinetochore-Microtubule Interactions in Fission Yeast
    J McIntosh; Fiscal Year: 2007
    ..The results from our work will inform biomedical science about important drug targets for cancer chemotherapy and genes that are significant for minimizing the risk of aneuploidy in both mitosis and meiosis. ..
  9. Kinetochore-Microtubule Interactions
    J McIntosh; Fiscal Year: 2009
    ..We may also help to identify proteins that are ideally suited to serve as targets for the discovery of new drugs that block cell division, one of the strategies that is currently used by some of the effective cancer chemotherapeutics. ..
  10. Kinetochore-Microtubule Interactions
    JOHN RICHARD MCINTOSH; Fiscal Year: 2010
    ..We may also help to identify proteins that are ideally suited to serve as targets for the discovery of new drugs that block cell division, one of the strategies that is currently used by some of the effective cancer chemotherapeutics. ..
  11. MICROTUBULE DYNAMICS IN VIVO
    J McIntosh; Fiscal Year: 1992
    ..The role of Ca-calmodulin will be explored. Suggestive results from experiments in vivo will be used to design polymerization experiments in vitro to analyze regulatory phenomena in a more specifically defined environment...
  12. MICROTUBULE DYNAMICS IN VIVO
    J McIntosh; Fiscal Year: 1991
    ..The role of Ca-calmodulin will be explored. Suggestive results from experiments in vivo will be used to design polymerization experiments in vitro to analyze regulatory phenomena in a more specifically defined environment...
  13. MICROTUBULE DYNAMICS IN VIVO
    J McIntosh; Fiscal Year: 1990
    ..The role of Ca-calmodulin will be explored. Suggestive results from experiments in vivo will be used to design polymerization experiments in vitro to analyze regulatory phenomena in a more specifically defined environment...
  14. KINESIN LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 1999
    ..Through this combination of studies we hope to identify the mitotic roles of several MT-dependent motor enzymes and learn how the enzymes interact with one another and with MT assembly to effect orderly chromosome motion. ..
  15. KINESIN-LIKE MOTORS IN MITOTIC MOVEMENTS
    J McIntosh; Fiscal Year: 1993
    ..Through this combination of studies we hope to identify the mitotic roles of several MT-dependent motor enzymes and learn how the enzymes interact with one another and with MT assembly to effect orderly chromosome motion...
  16. Kinetochore-Microtubule Interactions
    J McIntosh; Fiscal Year: 2009
    ..We may also help to identify proteins that are ideally suited to serve as targets for the discovery of new drugs that block cell division, one of the strategies that is currently used by some of the effective cancer chemotherapeutics. ..