Intracytoplasmic sperm injection (ICSI) enables rescue of valuable mutant mouse strainsMing-Wen Li
Murine Targeted Genomics Laboratory, Mouse Biology Program, Center for Comparative Medicine, University of California, Davis, California, USA
Comp Med 53:265-9. 2003
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- Enhanced effect of glycyl-L-glutamine on mouse preimplantation embryos in vitro
John D Biggers
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Reprod Biomed Online 9:59-69. 2004
..Recently, alanyl-L-glutamine has been used to replace glutamine in media for the culture of human preimplantation embryos. The results in this paper suggest that glycyl-L-glutamine may be a better choice of dipeptide...
- Discrepancies between the effects of glutamine in cultures of preimplantation mouse embryos
John D Biggers
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Reprod Biomed Online 9:70-3. 2004
..The finding that ammonium can also cause exencephaly thus fosters undue concern about the teratological effects of glutamine...
- Mouse embryo development following IVF in media containing either L-glutamine or glycyl-L-glutamine
M C Summers
Reproductive Science Center, Lexington, MA 02421, USA
Hum Reprod 20:1364-71. 2005
..CONCLUSIONS: There is no evidence that the presence of glutamine in the medium used for IVF influences significantly the subsequent development of the zygote. Replacing glutamine with glycyl-L-glutamine may be advantageous...
- Mouse sperm desiccated and stored in trehalose medium without freezing
Lynda K McGinnis
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Biol Reprod 73:627-33. 2005
..The results show that trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing...
- One-step versus two-step culture of mouse preimplantation embryos: is there a difference?
J D Biggers
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA, 02115 Beth Israel Deaconess Hospital, Boston, MA 02115, USA
Hum Reprod 20:3376-84. 2005
..A comparison has been made of the development of mouse zygotes in either one-step or two-step culture systems...
- Long-term storage of mouse spermatozoa after evaporative drying
Ming W Li
School of Veterinary Medicine, Center for Comparative Medicine, University of California, Davis, CA 95616, USA
Reproduction 133:919-29. 2007
..The possibility that the storage temperature must be less than the glass transition temperature is discussed...
- Desiccation tolerance of spermatozoa dried at ambient temperature: production of fetal mice
Sankha Bhowmick
Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston 02115, USA
Biol Reprod 68:1779-86. 2003
..05). The results demonstrate the feasibility of using a convective drying protocol for the successful desiccation of mouse sperm and identifies some of the important parameters required for optimization of the procedure...
- Evidence that glucose is not always an inhibitor of mouse preimplantation development in vitro
J D Biggers
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Hum Reprod 16:153-163. 2001
..The slight inhibitory effects of KH(2)PO(4) appeared to be due to the inhibition of the development of a few sensitive embryos. No significant effects of glucose and KH(2)PO(4) were observed on the total cell counts...
- Cyclin D2 is an FSH-responsive gene involved in gonadal cell proliferation and oncogenesis
P Sicinski
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge 02142, USA
Nature 384:470-4. 1996
..The hypoplasia seen in cyclin D2(-/-) ovaries and testes prompted us to examine human cancers deriving from corresponding tissues. We find that some human ovarian and testicular tumours contain high levels of cyclin D2 messenger RNA...
- Coordinate action of Wt1 and a modifier gene supports embryonic survival in the oviduct
J A Kreidberg
Department of Medicine, Children s Hospital, Boston, Massachusetts 02115, USA
Mol Reprod Dev 52:366-75. 1999
..Wt1 is therefore among a small subset of genes required for survival of the pre-implantation embryo, and appears to function non-autonomously...
Targeted disruption of mouse Yin Yang 1 transcription factor results in peri-implantation lethalityM E Donohoe
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell Biol 19:7237-44. 1999
..Our studies demonstrate an essential function for YY1 in the development of the mouse embryo...
- Amino acids and preimplantation development of the mouse in protein-free potassium simplex optimized medium
J D Biggers
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Biol Reprod 63:281-93. 2000
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- Mammalian oocytes are targets for prostaglandin E2 (PGE2) action
Diane M Duffy
Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA 23507, USA
Reprod Biol Endocrinol 8:131. 2010
..PGE2 actions on granulosa cells are essential for successful ovulation. The aim of the present study is to determine if PGE2 also acts directly at the oocyte to regulate periovulatory events...
Role of Fyn kinase in oocyte developmental potentialJinping Luo
Center for Reproductive Sciences, University of Kansas Medical Center, Kansas City, KS 66160, USA
Reprod Fertil Dev 22:966-76. 2010
..These defects cause developmental arrest during oocyte maturation and pronuclear congression...
- Localized activation of Src-family protein kinases in the mouse egg
Lynda K McGinnis
Department of Anatomy and Cell Biology, Center for Reproductive Sciences, University of Kansas Medical Center, 3901 Rainbow Blvd, Kansas City, KS 66160, USA
Dev Biol 306:241-54. 2007
..Furthermore, activation of these kinases in the mouse egg is limited to distinct regions and occurs at specific times after fertilization...
- Fyn kinase activity is required for normal organization and functional polarity of the mouse oocyte cortex
Jinping Luo
Center for Reproductive Sciences, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
Mol Reprod Dev 76:819-31. 2009
..This was associated with a delay in completion of meiosis II. Pronuclei, however, eventually formed and subsequent mitotic cleavages and blastocyst formation occurred normally...
- Multiple mechanisms of germ cell loss in the perinatal mouse ovary
Patricia Rodrigues
Department of Molecular and Integrative Physiology, Center for Reproductive Sciences, 3088 Kansas Life Science Innovations Center, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
Reproduction 137:709-20. 2009
..From these findings we suggest that multiple perinatal mechanisms establish the primordial follicle reserve in mice...
- Dicer1 is essential for female fertility and normal development of the female reproductive system
Xiaoman Hong
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA
Endocrinology 149:6207-12. 2008
..These studies implicate Dicer1/miRNA mediated posttranscriptional gene regulation in reproductive somatic tissues as critical for the normal development and function of these tissues and for female fertility...
- Dynamics of protein phosphorylation during meiotic maturation
Lynda K McGinnis
Department of Anatomy and Cell Biology, University of Kansas Medical Center, 3901 Rainbow Blvd, Mail Stop 3038, Kansas City, KS 66160, USA
J Assist Reprod Genet 27:169-82. 2010
..To ask whether distinct kinase signaling pathways mediate cytoplasmic or nuclear maturation of mouse oocytes and if in vitro maturation influences the distribution and timing of these phosphorylation events...
- Functions of Fyn kinase in the completion of meiosis in mouse oocytes
Lynda K McGinnis
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 3901 Rainbow Blvd, Mail Stop 3043, Kansas City, KS 66160, USA
Dev Biol 327:280-7. 2009
..Commonalities in meiotic spindle and chromosome alignment defects under these experimental conditions demonstrate a significant role for Fyn kinase activity in meiotic maturation...
- Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in mouse oocytes
Lynda K McGinnis
Department of Anatomy and Cell Biology, University of Kansas Medical School, Kansas City, Kansas 66160, USA
Mol Reprod Dev 78:33-47. 2011
..The results demonstrate that FER kinase plays a critical role in oocyte meiotic spindle microtubule dynamics and may have an additional function in GVBD...
- Protein tyrosine kinase signaling during oocyte maturation and fertilization
Lynda K McGinnis
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
Mol Reprod Dev 78:831-45. 2011
..Future studies will hopefully reveal the extent to which these factors impact clinical assisted reproductive techniques in domestic animals and humans...
