David J McGee

Summary

Affiliation: University of South Alabama
Country: USA

Publications

  1. ncbi request reprint The Helicobacter pylori flbA flagellar biosynthesis and regulatory gene is required for motility and virulence and modulates urease of H. pylori and Proteus mirabilis
    David J McGee
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA
    J Med Microbiol 51:958-70. 2002
  2. ncbi request reprint Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily
    David J McGee
    Department of Microbiology and Immunology, University of South Alabama, College of Medicine, Mobile, AL 36688, USA
    Eur J Biochem 271:1952-62. 2004
  3. pmc Colonization and inflammation deficiencies in Mongolian gerbils infected by Helicobacter pylori chemotaxis mutants
    David J McGee
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, 307 N University Blvd, Mobile, AL 36688, USA
    Infect Immun 73:1820-7. 2005
  4. pmc Genetic microheterogeneity and phenotypic variation of Helicobacter pylori arginase in clinical isolates
    Justin G Hovey
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, AL, USA
    BMC Microbiol 7:26. 2007
  5. pmc Characterization of Bacillus anthracis arginase: effects of pH, temperature, and cell viability on metal preference
    Ryan J Viator
    Department of Biology, University of South Alabama, Mobile, AL 36688, USA
    BMC Biochem 9:15. 2008
  6. pmc In vitro and in vivo complementation of the Helicobacter pylori arginase mutant using an intergenic chromosomal site
    Melanie L Langford
    Biology Department, University of South Alabama, Mobile, AL 36688, USA
    Helicobacter 11:477-93. 2006
  7. pmc Helicobacter pylori AlpA and AlpB bind host laminin and influence gastric inflammation in gerbils
    Olga A Senkovich
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, 1501 Kings Highway, Shreveport, LA 71130, USA
    Infect Immun 79:3106-16. 2011
  8. ncbi request reprint Helicobacter pylori thioredoxin is an arginase chaperone and guardian against oxidative and nitrosative stresses
    David J McGee
    Department of Microbiology and Immunology and Biological Sciences, University of South Alabama, Mobile, AL, USA
    J Biol Chem 281:3290-6. 2006
  9. pmc Transcriptional profiling of gastric epithelial cells infected with wild type or arginase-deficient Helicobacter pylori
    Songhee H Kim
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, Shreveport, LA 71130, USA
    BMC Microbiol 12:175. 2012
  10. pmc Helicobacter pylori arginase mutant colonizes arginase II knockout mice
    Songhee H Kim
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, Shreveport, LA 71130, USA
    World J Gastroenterol 17:3300-9. 2011

Collaborators

Detail Information

Publications18

  1. ncbi request reprint The Helicobacter pylori flbA flagellar biosynthesis and regulatory gene is required for motility and virulence and modulates urease of H. pylori and Proteus mirabilis
    David J McGee
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA
    J Med Microbiol 51:958-70. 2002
    ..pylori and P. mirabilis and possibly also in other motile, ureolytic bacteria...
  2. ncbi request reprint Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily
    David J McGee
    Department of Microbiology and Immunology, University of South Alabama, College of Medicine, Mobile, AL 36688, USA
    Eur J Biochem 271:1952-62. 2004
    ..The results indicate that H. pylori RocF is necessary and sufficient for arginase activity and has unparalleled features among the arginase superfamily, which may reflect the unique gastric ecological niche of this organism...
  3. pmc Colonization and inflammation deficiencies in Mongolian gerbils infected by Helicobacter pylori chemotaxis mutants
    David J McGee
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, 307 N University Blvd, Mobile, AL 36688, USA
    Infect Immun 73:1820-7. 2005
    ..Chemotaxis mutants may provide new insights into the steps involved in H. pylori pathogenesis...
  4. pmc Genetic microheterogeneity and phenotypic variation of Helicobacter pylori arginase in clinical isolates
    Justin G Hovey
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, AL, USA
    BMC Microbiol 7:26. 2007
    ..Arginase (RocF) hydrolyzes L-arginine to L-ornithine and urea, and urease hydrolyzes urea to carbon dioxide and ammonium, which can neutralize acid...
  5. pmc Characterization of Bacillus anthracis arginase: effects of pH, temperature, and cell viability on metal preference
    Ryan J Viator
    Department of Biology, University of South Alabama, Mobile, AL 36688, USA
    BMC Biochem 9:15. 2008
    ..The arginase from Bacillus anthracis is not well characterized; therefore, this arginase was investigated by a variety of strategies and the enzyme was purified...
  6. pmc In vitro and in vivo complementation of the Helicobacter pylori arginase mutant using an intergenic chromosomal site
    Melanie L Langford
    Biology Department, University of South Alabama, Mobile, AL 36688, USA
    Helicobacter 11:477-93. 2006
    ..pylori at relatively low frequencies, and chromosomally based approaches. Chromosomal complementation strategies are susceptible to polar effects and disruption of other H. pylori genes, leading to unwanted pleiotropic effects...
  7. pmc Helicobacter pylori AlpA and AlpB bind host laminin and influence gastric inflammation in gerbils
    Olga A Senkovich
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, 1501 Kings Highway, Shreveport, LA 71130, USA
    Infect Immun 79:3106-16. 2011
    ..Identification of laminin as the target of AlpA and AlpB will facilitate future investigations of host-pathogen interactions occurring during H. pylori infection...
  8. ncbi request reprint Helicobacter pylori thioredoxin is an arginase chaperone and guardian against oxidative and nitrosative stresses
    David J McGee
    Department of Microbiology and Immunology and Biological Sciences, University of South Alabama, Mobile, AL, USA
    J Biol Chem 281:3290-6. 2006
    ..Trx1 and arginase equip H. pylori with a "renox guardian" to overcome abundant nitrosative and oxidative stresses encountered during the persistence of the bacterium in the hostile gastric environment...
  9. pmc Transcriptional profiling of gastric epithelial cells infected with wild type or arginase-deficient Helicobacter pylori
    Songhee H Kim
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, Shreveport, LA 71130, USA
    BMC Microbiol 12:175. 2012
    ....
  10. pmc Helicobacter pylori arginase mutant colonizes arginase II knockout mice
    Songhee H Kim
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, Shreveport, LA 71130, USA
    World J Gastroenterol 17:3300-9. 2011
    ..To investigate the role of host and bacterial arginases in the colonization of mice by Helicobacter pylori (H. pylori)...
  11. pmc Helicobacter pylori lipopolysaccharide modification, Lewis antigen expression, and gastric colonization are cholesterol-dependent
    Ellen Hildebrandt
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, 1501 Kings Highway, Shreveport, LA 71130, USA
    BMC Microbiol 9:258. 2009
    ..We compared phenotypes and in vivo colonization ability of H. pylori grown in a defined, serum-free growth medium, F12 with 1 mg/ml albumin containing 0 to 50 mug/ml cholesterol...
  12. pmc Unique host iron utilization mechanisms of Helicobacter pylori revealed with iron-deficient chemically defined media
    Olga Senkovich
    Louisiana State University Health Sciences Center Shreveport, Department of Microbiology and Immunology, 1501 Kings Highway, Shreveport, LA 71130, USA
    Infect Immun 78:1841-9. 2010
    ..This novel strategy may have evolved to permit limited growth in host tissue during persistent colonization while excessive injury or iron depletion is prevented...
  13. pmc Role of the HefC efflux pump in Helicobacter pylori cholesterol-dependent resistance to ceragenins and bile salts
    Elizabeth A Trainor
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, MSB 2 225, 1501 Kings Highway, Shreveport, LA 71130, USA
    Infect Immun 79:88-97. 2011
    ..Thus, both cholesterol and the putative bile salt efflux pump HefC play important roles in H. pylori resistance to bile salts and ceragenins...
  14. pmc Nutritional requirements and antibiotic resistance patterns of Helicobacter species in chemically defined media
    Traci L Testerman
    Department of Microbiology and Immunology, Louisiana State University Health Sciences Center Shreveport, Room 2 221, Shreveport, LA 71130, USA
    J Clin Microbiol 44:1650-8. 2006
    ..The data also suggest that chemically defined media described herein could yield the growth of a wide range of Helicobacter spp., allowing a more detailed characterization of Helicobacter physiology and interactions with host cells...
  15. pmc Differential expression and transcriptional analysis of the alpha-2,3-sialyltransferase gene in pathogenic Neisseria spp
    Mathanraj Packiam
    Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
    Infect Immun 74:2637-50. 2006
    ..We conclude that differential Stase expression between N. gonorrhoeae and N. meningitidis is due at least in part to differential lst gene transcription...
  16. pmc Helicobacter pylori chemotaxis modulates inflammation and bacterium-gastric epithelium interactions in infected mice
    Susan M Williams
    Department of Environmental Toxicology ETOX, University of California at Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA
    Infect Immun 75:3747-57. 2007
    ..Because others have shown that in vivo adherence promotes inflammation, we propose a model in which H. pylori uses chemotaxis to guide it to a productive interaction with the stomach epithelium...
  17. ncbi request reprint Helicobacter pylori arginase inhibits T cell proliferation and reduces the expression of the TCR zeta-chain (CD3zeta)
    Jovanny Zabaleta
    Department of Pathology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    J Immunol 173:586-93. 2004
    ..Furthermore, arginase inhibitors reversed these events. These results suggest that H. pylori arginase is not only important for urea production, but may also impair T cell function during infection...
  18. pmc In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity
    Kylie J Nolan
    School of Microbiology and Immunology, The University of New South Wales, Australia
    Infect Immun 70:685-91. 2002
    ..Since nixA is required for full urease activity, the decreased fitness of the nixA mutant is likely due to reduced urease activity; however, pleiotropic effects of the mutation cannot be completely ruled out...