Research Topics
Species | Paul McCraySummaryAffiliation: University of Iowa Country: USA Publications
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Publications
Differential effects of cytokines and corticosteroids on toll-like receptor 2 expression and activity in human airway epitheliaAudra A Winder
Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Respir Res 10:96. 2009..The recognition of microbial molecular patterns via toll-like receptors (TLRs) is critical for mucosal defenses...
Efficient killing of inhaled bacteria in DeltaF508 mice: role of airway surface liquid compositionP B McCray
Departments of Pediatrics, Internal Medicine, and Occupational and Environmental Health, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Am J Physiol 277:L183-90. 1999..8 +/- 2.6 vs. 17.8 +/- 5.6 meq/l). Because ASL [Cl(-)] is low in normal and mutant mice, salt-sensitive antimicrobial factors, including MBD-1, may be normally active...- Altering α-dystroglycan receptor affinity of LCMV pseudotyped lentivirus yields unique cell and tissue tropismDouglas E Dylla
Genetics Ph, d, Program, Program in Gene Therapy, 240 EMRB, The University of Iowa Roy J, and Lucille A, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242 USA
Genet Vaccines Ther 9:8. 2011..We pseudotyped non-primate feline immunodeficiency virus (FIV) vectors using LCMV derived glycoproteins with high or low affinity to α-DG and evaluated their properties in vitro and in vivo... - Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirusPaul B McCray
Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
J Virol 81:813-21. 2007..This model of lethal infection with SARS-CoV should be useful for studies of pathogenesis and for the development of antiviral therapies... - Infection of human airway epithelia by SARS coronavirus is associated with ACE2 expression and localizationHong Peng Jia
University of Iowa, Iowa City 52242, USA
Adv Exp Med Biol 581:479-84. 2006 - Persistent expression of factor VIII in vivo following nonprimate lentiviral gene transferYubin Kang
240G EMRB, Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
Blood 106:1552-8. 2005..These findings demonstrate the utility of GP64-pseudotyped FIV lentiviral vectors for targeting hepatocytes to correct disorders associated with deficiencies of secreted proteins... - Disruption of the CFTR gene produces a model of cystic fibrosis in newborn pigsChristopher S Rogers
Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Science 321:1837-41. 2008..The pig model may provide opportunities to address persistent questions about CF pathogenesis and accelerate discovery of strategies for prevention and treatment... - In vivo gene transfer using a nonprimate lentiviral vector pseudotyped with Ross River Virus glycoproteinsYubin Kang
Program in Gene Therapy, Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
J Virol 76:9378-88. 2002..Our data support the utility of RRV glycoprotein-pseudotyped FIV lentiviral vectors for hepatocyte- and neuroglia-related disease applications... - Large-scale gene discovery in human airway epithelia reveals novel transcriptsTodd E Scheetz
Department of Pediatrics, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Physiol Genomics 17:69-77. 2004..This UniGene set should be useful for studies of pulmonary diseases involving the airway epithelium including cystic fibrosis, respiratory infections and asthma. It also provides a reagent for large-scale expression profiling... - Loss of cystic fibrosis transmembrane conductance regulator function produces abnormalities in tracheal development in neonatal pigs and young childrenDavid K Meyerholz
Department of Pathology, University of Iowa, Iowa City, 52242, USA
Am J Respir Crit Care Med 182:1251-61. 2010..Although airway abnormalities are common in patients with cystic fibrosis (CF), it is unknown whether they are all secondary to postnatal infection and inflammation, which characterize the disease... - Haemophilus influenzae forms biofilms on airway epithelia: implications in cystic fibrosisTimothy D Starner
The University of Iowa, Department of Pediatrics, 200 Hawkins Drive, Iowa City, IA 52242, USA
Am J Respir Crit Care Med 174:213-20. 2006..Bacteria biofilms are increasingly recognized as contributing to bacterial persistence and disease pathogenesis in CF... - ACE2 receptor expression and severe acute respiratory syndrome coronavirus infection depend on differentiation of human airway epitheliaHong Peng Jia
Department of Pediatrics, 240-G EMRB, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
J Virol 79:14614-21. 2005..These findings have implications for understanding disease pathogenesis associated with SARS-CoV and NL63 infections... - Expression and activity of beta-defensins and LL-37 in the developing human lungTimothy D Starner
Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
J Immunol 174:1608-15. 2005..Lower levels of HBD-2 may be one factor contributing to the increased susceptibility of premature infants to pulmonary infections... - CFTR DeltaF508 mutation has minimal effect on the gene expression profile of differentiated human airway epitheliaJoseph Zabner
Department of Internal Medicine, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, IA 52242, USA
Am J Physiol Lung Cell Mol Physiol 289:L545-53. 2005..Overall, the results indicate that CFTR dysfunction has little direct impact on airway epithelial gene expression in samples grown under these conditions... - Correlation between beta-defensin expression and induction profiles in gingival keratinocytesSophie Joly
Dows Institute for Dental Research, University of Iowa, Iowa City, IA 52242 1001, USA
Mol Immunol 42:1073-84. 2005.... - Gene transfer to respiratory epithelia with lentivirus pseudotyped with Jaagsiekte sheep retrovirus envelope glycoproteinPatrick L Sinn
Program in Gene Therapy, Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
Hum Gene Ther 16:479-88. 2005.... - Novispirin G10-induced lung toxicity in a Klebsiella pneumoniae infection modelKaren H Bartlett
Department of Occupational and Environmental Health, The University of Iowa, Iowa City, Iowa 52242, USA
Antimicrob Agents Chemother 47:3901-6. 2003..This finding establishes the importance of investigating candidate antimicrobial agents in an in vivo infection model... - Cystic fibrosis pigs develop lung disease and exhibit defective bacterial eradication at birthDavid A Stoltz
Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Sci Transl Med 2:29ra31. 2010.... - Rhesus theta-defensin prevents death in a mouse model of severe acute respiratory syndrome coronavirus pulmonary diseaseChristine L Wohlford-Lenane
Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
J Virol 83:11385-90. 2009..RTD-1-treated, SARS-CoV-infected mice displayed altered lung tissue cytokine responses 2 and 4 days postinfection compared to those of untreated animals, suggesting that one possible mechanism of action for RTD-1 is immunomodulatory... - Lentivirus vector can be readministered to nasal epithelia without blocking immune responsesPatrick L Sinn
Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
J Virol 82:10684-92. 2008..Prolonged gene expression has been observed in vivo following a single dose of virus vector; however, depending on the application, repeated administration of vector may be necessary to achieve stable, therapeutic gene expression... 
Microarray mRNA expression profiling to study cystic fibrosisShyam Ramachandran
Interdisciplinary Program in Genetics, Department of Pediatrics, University of Iowa, Iowa City, IA, USA
Methods Mol Biol 742:193-212. 2011..We also outline novel strategies for experimental design and data analysis, and describe some powerful gene and pathway discovery tools...- Ectodomain shedding of angiotensin converting enzyme 2 in human airway epitheliaHong Peng Jia
Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Am J Physiol Lung Cell Mol Physiol 297:L84-96. 2009..quot; Thus ACE2 must be cell associated to serve as a CoV receptor and soluble ACE2 might play a role in modifying inflammatory processes at the airway mucosal surface... - Enhanced gene expression conferred by stepwise modification of a nonprimate lentiviral vectorPatrick L Sinn
Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Hum Gene Ther 18:1244-52. 2007..These sequential modifications provide an improved FIV lentivirus platform for gene therapy applications and may be applied to other retroviral vectors... - MD-2-dependent pulmonary immune responses to inhaled lipooligosaccharides: effect of acylation stateSuzana Hadina
Department of Occupational and Environmental Health, The University of Iowa, Iowa City, IA 52242 5000, USA
Am J Respir Cell Mol Biol 38:647-54. 2008..Hyporesponsiveness in MD-2-null mice after nasal aspiration of wild-type LOS indicate its essential role in airway responsiveness to endotoxin... - The porcine lung as a potential model for cystic fibrosisChristopher S Rogers
Department of Internal Medicine, Roy J Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Am J Physiol Lung Cell Mol Physiol 295:L240-63. 2008..Thus pigs with a targeted CFTR gene might provide a good model for CF. Here, we review aspects of porcine airways and lung that are relevant to CF... - Integration site choice of a feline immunodeficiency virus vectorYubin Kang
Program in Gene Therapy, Department of Pediatrics, University of Iowa, Iowa City, 52242, 52242, USA
J Virol 80:8820-3. 2006..FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus... - Persistent gene expression in mouse nasal epithelia following feline immunodeficiency virus-based vector gene transferPatrick L Sinn
Program in Gene Therapy, Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, 52242, USA
J Virol 79:12818-27. 2005..These data suggest that AcGP64-FIV efficiently transduces and persistently expresses a transgene in nasal epithelia in the absence of agents that disrupt the cellular tight junction integrity... - Differential gene expression in human conducting airway surface epithelia and submucosal glandsAnthony J Fischer
Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
Am J Respir Cell Mol Biol 40:189-99. 2009..This study illustrates the power of LCM in defining the roles of specific anatomic features in airway biology and may be useful in examining how disease states alter transcriptional programs in the conducting airways... - Viral vector-mediated and cell-based therapies for treatment of cystic fibrosisTerence R Flotte
Department of Pediatrics, University of Florida, Gainesville, Florida, USA
Mol Ther 15:229-41. 2007..This article will review the advancements made and challenges remaining in the development of viral vector-mediated and cell-based approaches to treat patients with CF... - Lentivirus vectors pseudotyped with filoviral envelope glycoproteins transduce airway epithelia from the apical surface independently of folate receptor alphaPatrick L Sinn
Program in Gene Therapy, Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
J Virol 77:5902-10. 2003..Understanding viral vector binding and entry pathways is fundamental for developing cystic fibrosis gene therapy applications... - Human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganismsSophie Joly
Dows Institute for Dental Research, University of Iowa, Iowa City, Iowa 52242, USA
J Clin Microbiol 42:1024-9. 2004..Finally, the significant variability between strains underlies the importance of testing multiple strains when evaluating activities of antimicrobial peptides... - Innate immune functions of the airway epitheliumJennifer A Bartlett
Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Contrib Microbiol 15:147-63. 2008..We also provide examples of human disease states caused by impaired epithelial defenses in the lung... - PLUNC is a novel airway surfactant protein with anti-biofilm activityLokesh Gakhar
Department of Biochemistry and Protein Crystallography Facility, University of Iowa, Iowa City, Iowa, United States of America
PLoS ONE 5:e9098. 2010.... - Gene transfer to airway epithelia using feline immunodeficiency virus-based lentivirus vectorsGuoshun Wang
Departments of Pediatrics and Internal Medicine, Program in Gene Therapy, College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Methods Enzymol 346:500-14. 2002 - Susceptibility of nontypeable Haemophilus influenzae to human beta-defensins is influenced by lipooligosaccharide acylationTimothy D Starner
Department of Pediatrics, The University of Iowa, Iowa City, Iowa 52242, USA
Infect Immun 70:5287-9. 2002..In contrast, NTHI was more susceptible to HBD-3 and showed no changes in sensitivity as a result of lipooligosaccharide mutations in oligosaccharide and lipid A biosynthesis genes... - CCL20 is an inducible product of human airway epithelia with innate immune propertiesTimothy D Starner
Department of Pediatrics, University of Iowa Hospitals and Clinics, 200 Hawkins Drive, Iowa City, IA 52242, USA
Am J Respir Cell Mol Biol 29:627-33. 2003..Thus, CCL20 is a bi-functional peptide with both innate and adaptive immune properties that is regulated by inflammatory mediators, expressed by airway epithelia, and increased in cystic fibrosis airway secretions... - Cells of respiratory epitheliumPatrick L Sinn
Department of Pediatrics, University of Iowa, College of Medicine, Iowa City, USA
Methods Mol Biol 229:287-98. 2003 - A novel host defense system of airways is defective in cystic fibrosisPatryk Moskwa
Department of Anatomy, University of Iowa, Coralville, IA 52241, USA
Am J Respir Crit Care Med 175:174-83. 2007..Defective airway immunity characterizes cystic fibrosis (CF), a disease caused by mutations in the CF transmembrane conductance regulator, a chloride channel. The pathophysiology of defective immunity in CF remains to be elucidated... - Inclusion of Jaagsiekte sheep retrovirus proviral elements markedly increases lentivirus vector pseudotyping efficiencyPatrick L Sinn
Department of Pediatrics, Program in Gene Therapy, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
Mol Ther 11:460-9. 2005..These novel data demonstrate that optimization of the envelope expression construct can profoundly influence titers for lentivirus vectors... - Endotoxin responsiveness of human airway epithelia is limited by low expression of MD-2Hong Peng Jia
Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Am J Physiol Lung Cell Mol Physiol 287:L428-37. 2004..The regulation of MD-2 expression in airway epithelia and pulmonary macrophages may serve as a means to modify endotoxin responsiveness in the airway... - Viscoelastic gel formulations enhance airway epithelial gene transfer with viral vectorsPatrick L Sinn
Department of Pediatrics, 240G EMRB, The University of Iowa, Iowa City, IA 52242, USA
Am J Respir Cell Mol Biol 32:404-10. 2005..Importantly, viscoelastic gel formulations enhanced vector transduction to the conducting airways, the desired treatment target for diseases such as cystic fibrosis... - Measles virus preferentially transduces the basolateral surface of well-differentiated human airway epitheliaPatrick L Sinn
Department of Pediatrics, Program in Gene Therapy, The University of Iowa College of Medicine, Iowa City, IA 52242, USA
J Virol 76:2403-9. 2002..These data suggest that the progression of MV infection through the respiratory epithelium may involve pathways other than direct binding and entry through the apical surface of airway epithelia... - JunD protects the liver from ischemia/reperfusion injury by dampening AP-1 transcriptional activationJennifer J Marden
Molecular and Cellular Biology Interdisciplinary Graduate Program, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
J Biol Chem 283:6687-95. 2008..These findings suggest that JunD counterbalances JNK1 activation and the downstream redox-dependent hepatic injury that results from I/R, and may do so by regulating NADPH oxidases... - [beta]-defensins in lung host defenseBrian C Schutte
Department of Pediatrics, Genetics Ph.D. Program, University of Iowa College of Medicine, Iowa City, Iowa, USA
Annu Rev Physiol 64:709-48. 2002..Here we review much of the biology of the beta-defensins, including gene discovery, genomic organization, molecular structure, regulation of expression, and function... - Enhancement of respiratory mucosal antiviral defenses by the oxidation of iodideAnthony J Fischer
Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Am J Respir Cell Mol Biol 45:874-81. 2011..These results suggest that the LPO/I(-)/H(2)O(2) system can contribute to airway antiviral defenses. Furthermore, the delivery of I(-) to the airway mucosa may augment innate antiviral immunity... - Discovery of five conserved beta -defensin gene clusters using a computational search strategyBrian C Schutte
Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
Proc Natl Acad Sci U S A 99:2129-33. 2002..These results demonstrate the value of a genomewide search strategy to identify genes with conserved structural motifs. Discovery of these genes represents a new starting point for exploring the role of beta-defensins in innate immunity... - Reduction in the bactericidal activity of selected cathelicidin peptides by bovine calf serum or exogenous endotoxinKaren H Bartlett
Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, 100 Oakdale Campus, IREH, Iowa City, IA 52242, USA
Int J Antimicrob Agents 23:606-12. 2004..LPS-binding efficiency was not correlated with antimicrobial activity in complex media. Additionally, LPS- and serum-binding may interfere with the antimicrobial activity of peptides in complex media... - Cytokine-mediated regulation of antimicrobial proteinsJay K Kolls
Department of Pediatrics, Children s Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
Nat Rev Immunol 8:829-35. 2008..This suggests that this T-cell lineage and its cytokines have important roles in skin and mucosal immunity... - Impact of single-residue mutations on the structure and function of ovispirin/novispirin antimicrobial peptidesMonali V Sawai
Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242, USA
Protein Eng 15:225-32. 2002..These findings show that simple structural modifications can 'fine-tune' an antimicrobial peptide to minimize unwanted cytotoxicity while retaining its desired activity... - Pathogenesis of early lung disease in cystic fibrosis: a window of opportunity to eradicate bacteriaTimothy D Starner
University of Iowa, Iowa City, Iowa 52242, USA
Ann Intern Med 143:816-22. 2005 - Genomics-based approaches to gene discovery in innate immunityToddE Scheetz
Department of Pediatrics, The University of Iowa, Iowa City, IA 52242, USA
Immunol Rev 190:137-45. 2002..Such an approach may be readily adopted to address other questions of relevance to immunology... - PLUNC is a secreted product of neutrophil granulesJennifer A Bartlett
Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
J Leukoc Biol 83:1201-6. 2008..These studies suggest that PLUNC expression is less restricted than previously believed, and highlight new avenues of research for the study of PLUNC function... - Basolateral entry and release of New and Old World arenaviruses from human airway epitheliaDouglas E Dylla
The Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
J Virol 82:6034-8. 2008..Viral egress from infected epithelia exhibited basolateral polarity. This study demonstrates that respiratory entry of arenaviruses occurs via basolateral receptors... - Expression of beta-defensins in gingival health and in periodontal diseaseJohn Bissell
Dows Institute for Dental Research, Department of Periodontics, College of Dentistry, University of Iowa, IA 52242, USA
J Oral Pathol Med 33:278-85. 2004..001). CONCLUSIONS: High levels of HBD-3 mRNA expression in healthy tissues suggest a potentially important protective role for defensins in the host immune response to infection by periodontal pathogens... - Inactivation of human beta-defensins 2 and 3 by elastolytic cathepsinsClifford C Taggart
Pulmonary Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Center, Beaumont Hospital, Dublin, Ireland
J Immunol 171:931-7. 2003..In chronic lung diseases associated with infection, overexpression of cathepsins may lead to increased degradation of HBD-2 and -3, thereby favoring bacterial infection and colonization... - Antimicrobial peptides in animals and their role in host defencesKim A Brogden
Respiratory Diseases of Livestock Research Unit, US Department of Agriculture, Agricultural Research Service, National Animal Disease Center, PO Box 70, Ames, Iowa 50010, USA
Int J Antimicrob Agents 22:465-78. 2003..g. myeloid antimicrobial peptides, alpha-, beta-defensins, and protegrins) or are specific to one of these groups (e.g. porcine cecropin P1, Bac5, Bac7, PR-39 and prophenin)... - In vivo imaging of gene transfer to the respiratory tractUta Griesenbach
Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW3 6LR, UK
Biomaterials 29:1533-40. 2008..45, p<0.05). To our knowledge these studies show for the first time that this non-invasive method of assessing pulmonary gene transfer is viable for evaluating non-viral gene transfer agents... - Measles virus blind to its epithelial cell receptor remains virulent in rhesus monkeys but cannot cross the airway epithelium and is not shedVincent H J Leonard
Department of Molecular Medicine, Mayo Clinic, Rochester, Minnesota, USA
J Clin Invest 118:2448-58. 2008..Thus, EpR is probably a basolateral protein, and infection of the airway epithelium is not essential for systemic spread and virulence of MV... - Endogenous and exogenous glucocorticoid regulation of ENaC mRNA expression in developing kidney and lungKenzo Nakamura
Department of Internal Medicine, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City 52242, USA
Am J Physiol Cell Physiol 283:C762-72. 2002.... - The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureusDavid J Schibli
Structural Biology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada
J Biol Chem 277:8279-89. 2002.... - Developmental expression and distribution of sheep beta-defensin-2David K Meyerholz
Department of Veterinary Pathology, 2740 Veterinary Medicine, Iowa State University, Ames, IA 50011 1250, USA
Dev Comp Immunol 28:171-8. 2004..Interestingly, ileal follicle-associated epithelium lacked detectable SBD-2 immunoreactivity. SBD-2 mRNA and peptide expression are greatest in the intestinal tract and tissue distribution progressively decreases with maturity...
Research Grants
- Filovirus enveloped FIV:virus-epithelia interactions-CFPaul McCray; Fiscal Year: 2007..Completing these three aims will address key questions regarding host-vector interactions and improve our ability to apply lentiviral vectors for diseases involving airway epithelia. ..
- Enhancing hepatocyte gene transfer for Hemophilia APaul McCray; Fiscal Year: 2007..Completion of these aims will move us closer to clinical gene transfer application of FIV for the treatment of hemophilia A. ..
- RETROVIRUS MEDIATED GENE TRANSFER TO AIRWAY EPITHELIAPaul McCray; Fiscal Year: 2002..It is our goal to take advantage of this vector for lasting correction of CFTR deficiency. ..